IndraLab

Statements

OTUD5 is phosphorylated. 14 / 14
| 13 1
sparser
"Consistent with previous NMR studies on the isoform 1 of DUBA ( xref ), the chemical shifts of only a few residues near S177, including G174, Y175 and E178, are perturbed in the phosphorylated DUBA."
30232733
sparser
"With genetic rescue experiments we demonstrate that DUBA phosphorylation and catalytic activity are crucial in vivo for DUBA function in spermatogenesis."
27518434
sparser
"The crystal structure of the catalytic domain of the phosphorylated DUBA (p-DUBA) conjugated to ubiquitin-aldehyde revealed that the interactions between the phosphate group and the two positively charged residues, R272 and K273, in the α6 helix lead to the enclosure of the C-terminal tail of ubiquitin ( xref ). xref NMR studies show that phosphorylation induces minimal structural changes in the apo state of DUBA, xref raising the question of whether modulation of conformational dynamics plays a role in DUBA activation."
33417762
sparser
"The phosphorylated DUBA displays essentially the same predicted secondary structure, which is in good agreement with the crystal structures of both non-phosphorylated DUBA in free form (PDB ID: 3PFY) and phosphorylated DUBA covalently linked to ubiquitin aldehyde (PDB ID: 3TMP)."
30232733
sparser
"The crystal structure of the Ub-aldehyde complex with phosphorylated OTUD5 reveals a remarkable interaction between the phosphorylated Ser177 of OTUD5 and the guanidinium moiety of Arg74 of the bound ubiquitin showing that the phosphorylation is essential for the DUB activity [ xref ]."
32512887
sparser
"Therefore, modulating OTUD5 phosphorylation may be a promising strategy for tumor treatment."
32826889
sparser
"Moreover, phosphorylated OTUD5 was more efficient than wild-type OTUD5 in removing the ubiquitin chain of TRIM25 (Fig.  xref , lane 2 versus lane 3)."
32826889
sparser
"This is somewhat reminiscent of the activation mechanism of DUBA/OTUD5 by phosphorylation, which also depends on a UIM, yet in the case of DUBA phosphorylation activates a relatively less chain-specific DUB ( xref )."
29395066
sparser
"Consistently, our work indicated that phosphorylated OTUD5 is particularly efficient at cleaving the ubiquitin chains of TRIM25."
32826889
sparser
"A recent study using NMR and enzymatic kinetics of different forms of OTUD5 confirmed that only the phosphorylated OTUD5 is important for the activity [ xref ]."
32512887
rlimsp
"Huang and colleagues and Velázquez-Delgado and Hardy (this issue of Structure) describe how phosphorylation activates the protease activity of the deubiquitinating enzyme DUBA and how it inhibits caspase-6, respectively."
22483104
sparser
"By comparing NMR and enzyme kinetics data among different forms of DUBA with low and high activities, we concluded that a two-state equilibrium that was present only in phosphorylated DUBA is important for DUBA activity."
32071088
sparser
"Here we show that phosphorylation of the human deubiquitinase DUBA (OTUD5) at a single residue, Ser177, is both necessary and sufficient to activate the enzyme."
22245969
sparser
"xref shows the overlaid 1 H- 15 N TROSY spectra of phosphorylated and non-phosphorylated DUBA, which display only minor differences."
30232733