IndraLab

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USP10 binds Flag. 3 / 3
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"To determine whether ubiquitinated NP is specifically deubiquitinated by USP11, we co-transfected plasmids that can express NP-HA, Myc-Ub and Flag-USP10 or USP11, and then subjected HA-tagged immunoprecipitates to immunoblot analysis using the indicated antibodies ( Figure 5D )."
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"Moreover, ectopic expression of Flag-USP10 resulted in dose-dependent elevation of HA-HDAC7 in HEK-293T cells (Fig.  xref b), whereas USP10 knockdown markedly increased HDAC7 polyubiquitination and decreased the HDAC7 protein expression without influencing the HDAC7 mRNA levels in EC109 cells (Fig.  xref c, d)."
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"The NP-HA plasmid was replaced by PA-HA or PB2-HA, and visualized by immunoblot with anti-HA and anti-myc antibodies. (D) 293T cells were co-transfected with plasmids that can express NP-HA, Flag-USP10, Flag-USP11 and Myc-Ub as indicated."
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