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USP46 binds AS1. 25 / 25
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"Additionally, five adaptive lncRNAs, LINC01138, LINC00942, AL031985.3, AC015908.3 and USP46-AS1, were selected according to the performance of multivariate Cox regression analysis to construct the prognostic signature."
33764367
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"We found 24 overlaps between the two lists of ncRNAs, and three ncRNA signatures, namely USP46‐AS1 , PON2 and LOC441179 , as shown in Table  xref , remained after subjecting the 24 overlaps to the random survival forest model and were used for the establishment of a prognostic signature."
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"Survival rate is an important indicator of prognosis after treatment, and the significant association of survival rate with the expression levels of USP46‐AS1 , PON2 and LOC441179 suggests that those three ncRNAs should be important indicators for prognosis prediction for glioma patients."
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"Results of the log-rank test showed that the high expression groups of AC112220.2, AL391121.1, USP46-AS1, AL450326.1, SUCLG2-AS1, RAP2C-AS1, FGD5-AS1, AC018647.2 and AC015922.2 had significantly longer OS than their respective low expression groups (Figure xref )."
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"Meanwhile high AC112220.2, USP46-AS1, AL450326.1, SUCLG2-AS1, FGD5-AS1, AC018647.2 and AC015922.2 expression groups had remarkably longer DFS (Disease Free Survival) than their respective low expression groups."
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"Then the expression data of these 2697 lncRNAs and VHL was performed to WGCNA, results showed that 12 lncRNAs (FGD5-AS1, RAP2C-AS1, SUCLG2-AS1, USP46-AS1, AL391121.1, RP11-10C24.1, AC112220.2, AC018647.2, AC015922.2, MID1IP1-AS1, AL731577.2 and AL450326.1) were co-expressed with VHL (Table xref )."
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"We established the prognostic signature as a risk score formula by subjecting the three ncRNA expression values to multivariate Cox regression analysis in the training set as follows: risk score = −3.1563 × expression level of USP46‐AS1  + 0.3846 × expression level of PON2  + 0.4144 × expression level of LOC441179 ."
30984542
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"Meanwhile, results of the log-rank test also showed that the high AC112220.2, USP46-AS1, AL450326.1, SUCLG2-AS1, FGD5-AS1, AC018647.2 and AC015922.2 expression groups had remarkably longer DFS than their respective low expression groups (Figure xref )."
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"However, the relative expression of USP46‐AS1 in glioma cells was markedly decreased compared to the control cells ( P  <   0.05)."
30984542
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"Besides, the analysis results of catRAPID showed that AC112220.2, AL391121.1, USP46-AS1, AL450326.1, MID1IP1-AS1, SUCLG2-AS1, RAP2C-AS1, FGD5-AS1, AC018647.2 and AC015922.2 have a certain binding potential with VHL protein."
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"Besides this, random survival forest analysis identified a prognostic signature that is the weighted composition of expression values of three ncRNAs, namely LOC441179 , PON2 and USP46‐AS1 , that could accurately separate glioma samples with longer OS from those with shorter OS."
30984542
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"Furthermore, we used catRAPID to estimate the binding propensity of VHL protein with these 12 lncRNAs, and our results showed that AC112220.2, AL391121.1, USP46-AS1, AL450326.1, MID1IP1-AS1, SUCLG2-AS1, RAP2C-AS1, FGD5-AS1, AC018647.2 and AC015922.2 have a certain binding potential with VHL protein (Figure xref )."
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"qRT‐PCR was performed to investigate the expression of LOC441179 , PON2 and USP46‐AS1 in glioma cells compared to control cells."
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"Moreover, results of the log-rank test showed that high expression groups of AC112220.2, AL391121.1, USP46-AS1, AL450326.1, SUCLG2-AS1, RAP2C-AS1, FGD5-AS1, AC018647.2 and AC015922.2 had significantly longer OS than their respective low expression groups, and high AC112220.2, USP46-AS1, AL450326.1, SUCLG2-AS1, FGD5-AS1, AC018647.2 and AC015922.2 expression groups had remarkably longer DFS than their respective low expression groups."
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"In this study, we analyzed ncRNA expression profiles of 80 glioma samples from GEO and identified a ncRNA‐based prognostic signature composed of the weighted expressions of USP46‐AS1 , PON2 and LOC441179 ; based on this, glioma samples could be divided into high‐ and low‐risk groups with significantly different OS."
30984542
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"Results showed that 10 lncRNAs (AC112220.2, AL391121.1, USP46-AS1, AL450326.1, MID1IP1-AS1, SUCLG2-AS1, RAP2C-AS1, FGD5-AS1, AC018647.2 and AC015922.2) were identified as VHL-related lncRNAs, and they were down-regulated in ccRCC tissues."
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"Subsequently, five angiogenesis-associated lncRNAs, LINC01138, LINC00942, AL031985.3, AC015908.3 and USP46-AS1, were analyzed in the training set using univariate Cox regression analysis, Kaplan–Meier (KM) inspection and multivariate Cox regression analysis ( xref )."
33764367
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"In this study, we found that several lncRNAs, including FGD5-AS1, RAP2C-AS1, SUCLG2-AS1, USP46-AS1, AL391121.1, ENSG00000271020, AC112220.2, AC018647.2, AC015922.2, MID1IP1-AS1, AL731577.2 and AL450326.1 were co-expressed with VHL, and their expression were positively correlated with VHL expression."
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"Expression of LOC441179 , PON2 and USP46‐AS1 in glioma cells."
30984542
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"Expression values of both PON2 and LOC441179 were higher in patients with high risk score than in those with low risk score, while patients with high risk score tended to express a low USP46‐AS1 level (Fig.  xref C)."
30984542
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"This analysis has identified a prognostic signature of three lncRNAs, namely LOC441179, PON2, and USP46-AS1, which could separate GBM samples with longer overall survival from those with shorter survival [ xref ]."
32977537
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"Survival analysis results indicated that high expression groups of AC112220.2, AL391121.1, USP46-AS1, AL450326.1, SUCLG2-AS1, RAP2C-AS1, FGD5-AS1, AC018647.2 and AC015922.2 had significantly longer OS (Overall Survival) than their respective low expression groups."
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"Lower expression of FGD5-AS1, RAP2C-AS1, SUCLG2-AS1, AL391121.1, AC112220.2, AC018647.2, AC015922.2 and AL450326.1 were associated with advanced tumor stage (Figure xref A); lower expression of RAP2C-AS1 was related to lymphatic invasion (Figure xref B); lower expression of RAP2C-AS1, USP46-AS1, AC112220.2 and AC015922.2 tended to promote tumor metastasis (Figure xref C); lower FGD5-AS1, RAP2C-AS1, SUCLG2-AS1, USP46-AS1, AL391121.1, AC112220.2, AC018647.2, AC015922.2 and AL450326.1 expression levels were correlated with higher pathological stage (Figure xref D); lower FGD5-AS1, RAP2C-AS1, SUCLG2-AS1, USP46-AS1, AL391121.1, AC112220.2, AC018647.2 and AC015922.2 expression levels were also correlated with higher histological grade (Figure xref E)."
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"Kaplan–Meier analysis indicated that the patients with lower expression of LOC441179 and PON2 had a higher survival rate than patients with higher expression of LOC441179 and PON2 ; on the contrary, the down‐regulated expression of USP46‐AS1 was significantly associated with a higher survival rate."
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"Of those three ncRNAs, a higher expression value of LOC441179 (Fig.  xref A) and PON2 (Fig.  xref B) indicated shorter glioma OS, which corresponded to their positive estimated coefficients, while patients with a higher USP46‐AS1 (Fig.  xref C) expression value tended to have longer OS, which was consistent with its negative estimated coefficient."
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