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EGF activates phosphorylated ERK. 77 / 78
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"Furthermore, the study revealed that EGF upregulated the ratio of p-ERK/ERK in A549 cells, which was weakened by activin A, and A549 cell migration regulated by activin A was not related to calcium signaling."
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"The identification of 18 Affimers for 6 SH2 domains that reduced EGF-induced pERK translocation indicating they inhibited MAPK signalling demonstrates a hit rate of 22%."
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"Administration of EGF, however, markedly increased the up-regulation of phospho-ERK, ERCC1 and XRCC1 in both cell lines."
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"Unlike U937 cells, SKOV3 cells have low basal levels of pAkt and pERK, and pAkt and pERK can be stimulated by epidermal growth factor (EGF) treatment."
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"Comparing the EGF stimulated p-ERK bands without and with 50 nM siRNA concentrations clearly shows inhibition of phosphorylation with decreased IP 3 KB expression (comparing lanes 1, 2, 3 with 4, 5, 6)."
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"However, we found that SphK1 blockade with 1a did inhibit EGF stimulated accumulation of pAkt and pERK in SKOV3 cells."
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"We observed that, as it was expected XREF_BIBR, hSprouty2 wt or hSpry2 P59AP304A double mutant were unable to shut down the ERK and Elk1 signaling pathway after EGFR activation (XREF_FIG), whereas hSpred1 or hSpred2 reduced phospho-ERK levels stimulated by EGF (XREF_FIG)."
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"That effort (AID 1454) used AlphaScreen technology to assess levels of phosphorylated ERK in lysates of cells treated with EGF."
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"EGF increased p-ERK1/2, c-Fos, c-Jun, and p-c-Jun levels, which were inhibited by U0126."
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"EGF also upregulated p-ERK, increased Ki-67 positive cells, and decreased TUNEL positive cells."
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"We analyzed the EGF induced distribution of p-ERK in young HDFs transfected with siRNA1."
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"As results, p-EGFR, p-AKT, and p-ERK were significantly increased by treatment of EGF compared with those of untreated cells."
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"ERK1/2 agonist EGF was found to antagonize F 2 inhibition of calcium-free-H and R-induced p-ERK1/2 upregulation in cardiomyocytes."
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"We next visualized diphosphorylated ERK (dpERK), which is activated by EGF signaling, in kek1 and wildtype embryos."
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"The ERK1/2 activator EGF antagonized F 2 inhibition of calcium-free-H and R-induced p-ERK1/2 upregulation and inhibited F 2 protection of cardiomyocytes."
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"U0126, a MEK inhibitor, inhibited the EGF-induced elevation of p-ERK1/2 ( Fig. 2 A)."
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"In contrast, LY-294002, a PI3-K inhibitor, did not affect the EGF-induced elevation of p-ERK1/2."
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"Expression of either Spry4 proteins failed to influence the pERK profile mediated by EGF (XREF_FIG)."
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"EGF induced significant activation of pEGFR (Tyr 1068) and pERK, along with significant downregulation of EGFR to undetectable levels after 3 h of treatment."
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"In agreement with these observations, EGF enhanced p-ERK1/2 in wt cells, suggesting that EGF transduced cell proliferative signals via ERK activation [53,55,56]."
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"OTP-3 exerted no effect on activation of pEGFR (Tyr 1068), pERK and pAKT by EGF but specifically augmented ligand-induced downregulation of total EGFR protein."
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"Technical replicates of the same two lysates showed good reproducibility in the observed EGF stimulated induction of p-ERK1/2, and loading controls were consistent across the membrane (XREF_FIG)."
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"In contrast, siYAP and TAZ did not inhibit increases in pERK induced by EGF."
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"It appears that the extent of pERK activation by EGF in CapZ KO cells is greater than in control cells (Author response image 4B)."
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"Thus, EGF (and other growth factors applied in a similar manner) induced pERK in proportion to the degree of BRAF inhibition."
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"These data suggest that EGF-mediated p-ERK induction in NF1-null cells is not dependent on SOS1 activity.To determine the relative sensitivity of these different melanoma genotypes to MAPK pathway inhibition, we used three allosteric MEK inhibitors with distinct biochemical properties.12 13 The first, trametinib, promotes the dissociation of RAF-MEK1/2 complexes, antagonizing MEK1 and MEK2 phosphorylation at serine 218."
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"For example, EGF stimulation induces the entry of p-ERK into the nucleus, which results in the activation of the transcription factor AP1, triggering cell cycle progression."
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"Moreover, EGF treatment of Panc-1 CBL-low cells also resulted in increased activation of pERK and pAKT (XREF_FIG, columns 2 vs. 4); these downstream mediators were not activated in Panc-1 CBL-low cells in the absence of exogenous ligand."
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"However, it raises the question: why is pERK only transiently activated by EGF in drug adapted cells if feedback is suppressed?"
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"However, it raises the question: why is pERK only transiently activated by EGF in drug adapted cells if feedback is suppressed?"
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"EGF induced significant activation of pEGFR (Tyr 1068) and pERK, along with significant downregulation of EGFR to undetectable levels after 3 h of treatment."
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"Upon EGF, MSI2 overexpression promoted EGF induced decrease of E-cad, ZO-1 and beta-catenin and enhanced EGF induced increase of c-Myc, pERK and Vimentin, all which were reversed by MSI2siRNA."
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"In parental cells, EGF stimulation caused robust induction of pERK within 5 min and was downregulated by 60 min, presumably due to the induction of negative feedback loops ( Figures 6 A–6C )."
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"In BxPC-3, ERL appropriately reduced EGF stimulated pEGFR and pERK at 10 ng/ml, whereas BYL reduced IGF stimulated pAkt signals at 50 ng/ml."
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"The strong activation of p-ERK by EGF at both 10 and 30 min post-stimulation results in the p-ERK coefficients projecting towards the EGF scores (compare in Fig. 1C and 1D), while the strong activation of the p38 pathway by IL-1α and TNFα results in the p-p38 coefficients projecting towards the IL-1α and TNFα scores (compare in Fig. 1C and 1D)."
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"Earlier studies have reported that non covalent nucleotide exchange inhibitors can reduce EGF stimulated pERK signalling through wild-type Ras."
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"Even within a single ligand family, substantial differences in relative responsiveness were observed : HRG and EGF induced pERK to the same degree in BT-20 and T47D cells, but HRG was much more potent than EGF in CAMA-1 and MCF-7 cells."
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"Differences were also observed with respect to which downstream kinases were activated : BT-20 and T47D cells were highly responsive to EGF at the level of pERK and pAKT, whereas EGF induced only pERK in CAMA-1 and MCF-7 cells."
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"We found that whereas EGF elicited symmetric pERK or pAKT induction, the FGF-1 response was heavily biased towards pERK, and the response of IGF-1 was biased to pAKT (solid lines in Figure XREF_FIG A and distributions in Figure XREF_FIG B)."
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"This suggests that, for fold-change induction of pERK by EGF, EGFR is a positive regulator, pErbB2 a negative regulator and ERK level does not matter."
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"Mg 2+ deprivation decreased p-ERK1/2 levels in the FCS-containing medium and inhibited the sustained phase of p-ERK1/2 caused by EGF ( Figs."
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"Activation of RAS, p-MEK and p-ERK by EGF was more transient in LIM1215 cells lacking ARAF than in controls, and ARAF overexpression prolonged activation ( xref and xref )."
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"To test this, we quantified phospho-ERK induced by EGF stimulation in the presence or absence of rapamycin or iRap."
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"For example, sensitive parameters for pERK activation by EGF or HRG stimulation were largely shared (XREF_FIG)."
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"The set of sensitive parameters common to both EGF mediated pERK and pAkt induction was limited to GAP and ErbB1 initial concentrations (XREF_FIG)."
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"On the other hand, EGF induced a strong and comparable pAKT(S473) response in A549 , A549 , and A549 (Figure 5B), and a weaker but reproducible pERK response (Supplemental Figure S5D)."
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"On the other hand, the pan-siRNA treatment significantly reduced the EGF-induced pERK response (Supplemental Figure S6B)."
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"NGF, PACAP, EGF, and anisomycin induced distinct temporal patterns and combinations of pERK, pJNK, pp38, and pCREB and the IEGs (XREF_FIG)."
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"Epidermal growth factor (15 nM) potentiated pERK in the presence of 7.5 mM glucose in control and RyR2 cells, but this potentiation was abolished in IRBIT cells (Fig. 5d)."
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"It is well known that epidermal growth factor (EGF) stimulation causes a significant increase in pERK, and this effect is observed even following EGF withdrawal."
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"Immunoblot analysis revealed that 2 nM EPR stimulated a six-fold increase in p-ERK1/2, whereas 10 nM EGF or TGFalpha stimulated only a 3- and 2.5-fold increase in p-ERK1/2."
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"The strong activation of p-ERK by EGF at both 10 and 30 min post-stimulation results in the p-ERK coefficients projecting towards the EGF scores (compare in xref ), while the strong activation of the p38 pathway by IL-1α and TNFα results in the p-p38 coefficients projecting towards the IL-1α and TNFα scores (compare in xref )."
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"Incubation with SkQ1 suppressed EGF induced p-ERK1/2 upregulation in SiHa, but not in Ca-Ski cells."
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"EGF induced higher phosphor-EGFR expression and upregulated p-ERK in HCECs."
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"It is known that EGF stimulation causes a significant increase in pERK and that this effect is observed even following EGF withdrawal."
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"EGF additionally enhanced pERK in both cell lines."
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"EGF treatment rapidly and robustly increased p-ERK in 14-day-old differentiated cells."
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"Activation of RAS, p-MEK and p-ERK by EGF was more transient in LIM1215 cells lacking ARAF than in controls, and ARAF overexpression prolonged activation (Figures S4B and S4C)."
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"EGF was then used to rescue the p-ERK deficiency in per1b -/- zebrafish [41]."
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"The EGF induced pERK was blocked (gefitinib and vehicle : 16-93%) by gefitinib, with two cell lines (778 : p = 0.0007 and 449B : p = 0.0028) having statistical significance."
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"In control cells, HRG induced p-Akt, whereas EGF induced p-ERK1/2."
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"The resilience of the FHL124 cells is shown by the fact that, in the absence of Tg, the second pulse of EGF induces a pERK signal that is of the same magnitude as the first pulse after 24 hr.The overa[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"EGF induced intense activation of pEGFR (tyr1173, tyr 1045) and pERK."
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"EGF increased p-ERK1/2 2.9-fold (P < 0.01) in pLE cells and that this effect of EGF was completely inhibited by pre-treatment of U0126 at all concentrations tested."
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"We also found that over-expression of BRD7 can inactivate the c-jun and p-ERK1/2 after being treated with EGF in HNE1 cells."
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"Furthermore, combined application of U0126 and actinomycin D did not produce an additive effect, suggesting that EGF-mediated stimulation of pERK underlies channel synthesis.We next investigated how i[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"After two hours of RAF inhibition, however, EGF significantly stimulated pMEK and pERK (lane 6)."
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"The ability of EGF treatment to increase p-ERK1/2 in PLX4720-treated ES2ΔVe cells indicates that VEPH1 may inhibit a BRAF-independent EGF-induced MEK activation pathway."
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"Another common approach is to perform a F92A/V94A substitution, which has been used for a range of studies including insulin-mediated Cav-1 phosphorylation ( Kimura et al., 2002 ), negative regulation[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Phosphorylated ERK1/2, which was activated by EGF, promoted LF proliferation by accelerating cell-cycle progression from the G1 to S phase."
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"In TSC2-knockout mice that were treated with rapamycin, pERK was456 Biotechnic & Histochemistry 2017, 92 (6) : 450-458 unaffected by various stimulants, but pAKT was increased by EGF (Zhang et al. 2007)."
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"Most of the compounds in this series displayed double digit nanomolar IC 50 values in the MEK-1 enzymatic assay and double digit nanomolar EC 50 values against EGF-stimulated pERK MDA-MB-231 cancer ce[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"The activation of human epidermal growth factor receptor-2 (HER2) results in the activation of the mitogen activated protein kinase (MAPK) cascade that may lead to the resistance to anti-estrogen therapy in estrogen receptor (ERalpha) expressing breast cancer by means of phosphorylation of ERalpha in the N-terminal region by phosphorylated extracellular signal regulated kinase 1/2 (pERK1/2) and by means of decreasing ERalpha expression."
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"These results suggest that in VSMCs, Rho-kinase is a downstream target of EGF receptor through MAPK/Erk1/2 signaling.To confirm that stimulation of EGF receptor triggers activation of Rho-kinase throu[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Our results demonstrate that treatment of LacZ+, GFAP+ and PCNA+ 12 V-Ras Tg transformed astrocytes with SF1126 and LY294002 blocked the activation of AKT as well as EGF induced phospho-ERK."
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"In contrast, no change in EGF induced ERK cascade activation was detected at the 2 hr time point, whereas at the 18 hr time point, the levels of EGF induced pMEK, pERK, and Raf dimerization were dramatically reduced."
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"However, HIP1R knockdown suppressed the enhancement of ERK phosphorylation and the p-ERK/ERK ratio induced by EGF, with or without BIBX (Figures 6A,I,J)."
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