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EGF activates phosphorylated ERK. 57 / 58
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"EGF also upregulated p-ERK, increased Ki-67 positive cells, and decreased TUNEL positive cells."
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"We analyzed the EGF induced distribution of p-ERK in young HDFs transfected with siRNA1."
20833136
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"To test this, we quantified phospho-ERK induced by EGF stimulation in the presence or absence of rapamycin or iRap."
20154678
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"ERK1/2 agonist EGF was found to antagonize F 2 inhibition of calcium-free-H and R-induced p-ERK1/2 upregulation in cardiomyocytes."
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"Unlike U937 cells, SKOV3 cells have low basal levels of pAkt and pERK, and pAkt and pERK can be stimulated by epidermal growth factor (EGF) treatment."
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"EGF treatment rapidly and robustly increased p-ERK in 14-day-old differentiated cells."
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"The EGF induced pERK was blocked (gefitinib and vehicle : 16-93%) by gefitinib, with two cell lines (778 : p = 0.0007 and 449B : p = 0.0028) having statistical significance."
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"For the EGFRn-side binder, the dose-dependent reduction in pERK signalling elicited by 1 nM EGF in HUVECs with increasing EGFRn-side minibinder (EGFRn_mb) concentration is shown."
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"That effort (AID 1454) used AlphaScreen technology to assess levels of phosphorylated ERK in lysates of cells treated with EGF."
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"Technical replicates of the same two lysates showed good reproducibility in the observed EGF stimulated induction of p-ERK1/2, and loading controls were consistent across the membrane (XREF_FIG)."
25852189
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"For example, sensitive parameters for pERK activation by EGF or HRG stimulation were largely shared (XREF_FIG)."
19156131
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"Differences were also observed with respect to which downstream kinases were activated : BT-20 and T47D cells were highly responsive to EGF at the level of pERK and pAKT, whereas EGF induced only pERK in CAMA-1 and MCF-7 cells."
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"EGF induced intense activation of pEGFR (tyr1173, tyr 1045) and pERK."
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"It appears that the extent of pERK activation by EGF in CapZ KO cells is greater than in control cells (Author response image 4B)."
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"We found that whereas EGF elicited symmetric pERK or pAKT induction, the FGF-1 response was heavily biased towards pERK, and the response of IGF-1 was biased to pAKT (solid lines in Figure XREF_FIG A and distributions in Figure XREF_FIG B)."
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"The set of sensitive parameters common to both EGF mediated pERK and pAkt induction was limited to GAP and ErbB1 initial concentrations (XREF_FIG)."
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"In contrast, siYAP and TAZ did not inhibit increases in pERK induced by EGF."
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"Our results demonstrate that treatment of LacZ+, GFAP+ and PCNA+ 12 V-Ras Tg transformed astrocytes with SF1126 and LY294002 blocked the activation of AKT as well as EGF induced phospho-ERK."
25425962
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"The activation of human epidermal growth factor receptor-2 (HER2) results in the activation of the mitogen activated protein kinase (MAPK) cascade that may lead to the resistance to anti-estrogen therapy in estrogen receptor (ERalpha) expressing breast cancer by means of phosphorylation of ERalpha in the N-terminal region by phosphorylated extracellular signal regulated kinase 1/2 (pERK1/2) and by means of decreasing ERalpha expression."
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"In addition, it was observed that Tan IIA decreased the expression levels of phosphorylated (p)-Akt and p-ERK1/2 induced by EGF and TGF-beta1."
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"The strong activation of p-ERK by EGF at both 10 and 30 min post-stimulation results in the p-ERK coefficients projecting towards the EGF scores (compare in Fig. 1C and 1D), while the strong activation of the p38 pathway by IL-1α and TNFα results in the p-p38 coefficients projecting towards the IL-1α and TNFα scores (compare in Fig. 1C and 1D)."
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"NGF, PACAP, EGF, and anisomycin induced distinct temporal patterns and combinations of pERK, pJNK, pp38, and pCREB and the IEGs (XREF_FIG)."
23469182
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"Phosphorylated ERK1/2, which was activated by EGF, promoted LF proliferation by accelerating cell-cycle progression from the G1 to S phase."
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"EGF additionally enhanced pERK in both cell lines."
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"EGF was then used to rescue the p-ERK deficiency in per1b -/- zebrafish [41]."
29958979
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"Immunoblot analysis revealed that 2 nM EPR stimulated a six-fold increase in p-ERK1/2, whereas 10 nM EGF or TGFalpha stimulated only a 3- and 2.5-fold increase in p-ERK1/2."
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"This suggests that, for fold-change induction of pERK by EGF, EGFR is a positive regulator, pErbB2 a negative regulator and ERK level does not matter."
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"However, HIP1R knockdown suppressed the enhancement of ERK phosphorylation and the p-ERK/ERK ratio induced by EGF, with or without BIBX (Figures 6A,I,J)."
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"After two hours of RAF inhibition, however, EGF significantly stimulated pMEK and pERK (lane 6)."
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"These results suggest that in VSMCs, Rho-kinase is a downstream target of EGF receptor through MAPK/Erk1/2 signaling.To confirm that stimulation of EGF receptor triggers activation of Rho-kinase throu[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"The strong activation of p-ERK by EGF at both 10 and 30 min post-stimulation results in the p-ERK coefficients projecting towards the EGF scores (compare in xref ), while the strong activation of the p38 pathway by IL-1α and TNFα results in the p-p38 coefficients projecting towards the IL-1α and TNFα scores (compare in xref )."
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"In BxPC-3, ERL appropriately reduced EGF stimulated pEGFR and pERK at 10 ng/ml, whereas BYL reduced IGF stimulated pAkt signals at 50 ng/ml."
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"In agreement with these observations, EGF enhanced p-ERK1/2 in wt cells, suggesting that EGF transduced cell proliferative signals via ERK activation [53,55,56]."
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"d, Titration curve for EGF mediated pERK/pAKT signaling."
35332283
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"We next visualized diphosphorylated ERK (dpERK), which is activated by EGF signaling, in kek1 and wildtype embryos."
27379389
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"Expression of either Spry4 proteins failed to influence the pERK profile mediated by EGF (XREF_FIG)."
33670044
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"Upon EGF, MSI2 overexpression promoted EGF induced decrease of E-cad, ZO-1 and beta-catenin and enhanced EGF induced increase of c-Myc, pERK and Vimentin, all which were reversed by MSI2siRNA."
31952541
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"It is well known that epidermal growth factor (EGF) stimulation causes a significant increase in pERK, and this effect is observed even following EGF withdrawal."
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"It is known that EGF stimulation causes a significant increase in pERK and that this effect is observed even following EGF withdrawal."
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"Moreover, EGF treatment of Panc-1 CBL-low cells also resulted in increased activation of pERK and pAKT (XREF_FIG, columns 2 vs. 4); these downstream mediators were not activated in Panc-1 CBL-low cells in the absence of exogenous ligand."
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"EGF increased p-ERK1/2, c-Fos, c-Jun, and p-c-Jun levels, which were inhibited by U0126."
19937979
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"EGF induced higher phosphor-EGFR expression and upregulated p-ERK in HCECs."
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"Earlier studies have reported that non covalent nucleotide exchange inhibitors can reduce EGF stimulated pERK signalling through wild-type Ras."
28706291
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"In contrast, no change in EGF induced ERK cascade activation was detected at the 2 hr time point, whereas at the 18 hr time point, the levels of EGF induced pMEK, pERK, and Raf dimerization were dramatically reduced."
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"However, we found that SphK1 blockade with 1a did inhibit EGF stimulated accumulation of pAkt and pERK in SKOV3 cells."
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"We observed that, as it was expected XREF_BIBR, hSprouty2 wt or hSpry2 P59AP304A double mutant were unable to shut down the ERK and Elk1 signaling pathway after EGFR activation (XREF_FIG), whereas hSpred1 or hSpred2 reduced phospho-ERK levels stimulated by EGF (XREF_FIG)."
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"Administration of EGF, however, markedly increased the up-regulation of phospho-ERK, ERCC1 and XRCC1 in both cell lines."
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"As results, p-EGFR, p-AKT, and p-ERK were significantly increased by treatment of EGF compared with those of untreated cells."
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"The ERK1/2 activator EGF antagonized F 2 inhibition of calcium-free-H and R-induced p-ERK1/2 upregulation and inhibited F 2 protection of cardiomyocytes."
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"Even within a single ligand family, substantial differences in relative responsiveness were observed : HRG and EGF induced pERK to the same degree in BT-20 and T47D cells, but HRG was much more potent than EGF in CAMA-1 and MCF-7 cells."
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"EGF increased p-ERK1/2 2.9-fold (P < 0.01) in pLE cells and that this effect of EGF was completely inhibited by pre-treatment of U0126 at all concentrations tested."
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"We also found that over-expression of BRD7 can inactivate the c-jun and p-ERK1/2 after being treated with EGF in HNE1 cells."
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"Comparing the EGF stimulated p-ERK bands without and with 50 nM siRNA concentrations clearly shows inhibition of phosphorylation with decreased IP 3 KB expression (comparing lanes 1, 2, 3 with 4, 5, 6)."
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"In control cells, HRG induced p-Akt, whereas EGF induced p-ERK1/2."
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"In contrast, no change in EGF induced ERK cascade activation was detected at the 2 hr time point, whereas at the 18 hr time point, the levels of EGF induced pMEK, pERK, and Raf dimerization were drama[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Incubation with SkQ1 suppressed EGF induced p-ERK1/2 upregulation in SiHa, but not in Ca-Ski cells."
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"For example, EGF stimulation induces the entry of p-ERK into the nucleus, which results in the activation of the transcription factor AP1, triggering cell cycle progression."
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