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PTEN decreases the amount of PTEN. 85 / 108
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"Bioinformatics analysis using the STITCH database predicted that ETO could interact with WWP2 and PTEN by upregulating the protein expression of WWP2 and downregulating the protein expression of PTEN."
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"Notably, suppressing FAPalpha in oral squamous cancer cells significantly decreased the expression of phosphorylated phosphatidylinositol-3-kinase (PI3K), protein kinase B (AKT), mitogen activated protein kinase kinase 1/2 and extracellular signal regulated kinase (ERK) 1/2, and upregulated the expression of phosphatase and tensin homolog (PTEN)."
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"Decreased PTEN expression did not reduce RAD51 expression or clonogenic survival following PARPi among prostate cancer cells that vary in TP53 and PTEN."
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"Together, these data illustrate that the Pten m3m4 mutation results in decreased Pten nuclear localization and decreased Pten overall expression and leads to dysregulation in downstream canonical signaling in NSCs."
32487265
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"These results demonstrate that PTEN negatively regulates HBV replication, implying that HBV reduces PTEN expression to upregulate proviral activity."
32394474
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"Moreover, SNHG20 was confirmed to interact with PTEN, which negatively regulated PTEN protein level."
30690477
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"On the other hand, lnc-DILC recruited deubiquitinase USP11 to PTEN, thus reducing the ubiquitination level of PTEN."
31592114
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"Specifically, phosphatase and tensin homolog small interfering RNA (ExoPTEN)-loaded exosomes reduced the expression of PTEN, thereby improving structural and electrophysiological function in spinal cord injury."
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"The latter is in line with the rate of up to 50% PTEN " reduced expression " previously reported in conventional urothelial carcinoma [XREF_BIBR, XREF_BIBR] and our prior study finding of low H score of PTEN expression in urothelial carcinoma with divergent histology [XREF_BIBR]."
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"By western blot analysis, we observed that PTEN inhibited basal AKT phosphorylation, but not ERK1/2 phosphorylation in PTEN expressing cells."
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"As shown in Fig 3 D, transfection of PTEN shRNA could significantly suppress the expression of PTEN."
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"In particular, in AML and CML PTEN-tail phosphorylation was associated with PTEN inactivation and in APL, NPC+ AML and CML, PTEN nuclear exclusion promotes loss of the essential nuclear tumor suppressive functions of PTEN."
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"In addition HOTAIR represses the expression of phosphatase and tensin homolog (PTEN), an inhibitor of EMT [XREF_BIBR, XREF_BIBR]."
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"PTEN and/or PTENP1 depletion in DU145 cells decreased PTEN protein expression, which was similar to the original study."
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"PTEN inhibits PI3K, whereas a mutated PTEN gene, the presence of a pseudogene or other complex downregulation mechanisms, leads to decreased or inactive PTEN protein expression, thus PI3K overexpression and as such favors the proto-oncogene MYC 's action on cell cycle and survival."
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"PTEN shRNA significantly reduced the expression of PTEN protein in bAMs (XREF_FIG)."
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"In hNSCs, the delivery of four PTEN shRNAs reduced PTEN mRNA levels relative to empty vector control, but not significantly (0.65-fold, p = 0.34)."
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"Downregulation of PTEN phosphatase or constitutive PI3K activation are demonstrated resistance mechanisms for anti-HER2 therapies and decreased PTEN expression and activating mutations of PI3K are present in between 13-52% and 13-30% of her2 positive breast cancer, respectively [XREF_BIBR]."
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"In the present study, the findings from the Western blotting and methylation specific polymerase chain reaction (PCR) (MSP) assay, showed that DNMT1 expression promoted PTEN methylation, which might lead to reduced expression of PTEN."
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"This analysis of prospectively treated patients has several limitations, including the small sample size, the heterogeneity of prior and current therapy regimens including monotherapy and combination immunotherapy regimens, and the lack of functional validation that the observed PTEN alterations, which included single copy deletions, led to decreased PTEN expression in tumors."
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"Unlike p53 and Egr-1, two positive regulators of PTEN, SLUG negatively regulates PTEN expression by binding directly to the two E-boxes in its promoter region."
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"In Eca109 cells, cells treated with miR-301 mimic upregulated miR-301, whereas downregulated PTEN; cells treated with si-PTEN decreased PTEN expression, and pcDNA-PTEN (oe-PTEN) reversed the inhibitive role of miR-301 mimic in PTEN expression."
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"PTEN siRNA abrogated the expression of PTEN and P27, and increased the expression of P-AKT (Ser473), cyclinE1, cyclinA and CDK2 in both cell lines."
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"Furthermore, PTEN siRNA reduced the microinfarct volume, improved cardiac function (LVEF), reduced the release of cTnI, and suppressed PTEN and TNF-alpha protein expression."
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"While PTEN siRNA treatment reduced PTEN protein levels to a lesser degree in A375-ODAM cells, AKT phosphorylation was increased."
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"PTEN siRNAs (Si A and Si B) successfully suppressed basal PTEN expression by 43.9% and 33.9%, respectively (XREF_FIG)."
24284390
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"The increased miR-23a-3p in cells specially targeted the 3 ' UTR of PTEN by RNA induced silencing complex (RISC) and suppressed the level of PTEN."
32939233
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"Since PTEN levels are consistently increased in TMEPAI deficient cells and decreased in TMEPAI overexpressing cells, we tested if TMEPAI promotes PTEN turnover to reduce PTEN levels."
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"These results suggest that DNMT1 mediated PTEN hypermethylation caused the loss of PTEN expression, followed by the activation of the PI3K and AKT and ERK pathways, resulting in HSC activation."
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"The expression of PTEN protein in MCF-7 cells was effectively blocked by PTEN antisense oligonucleotide."
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"Consistent with previous report (25), OA reduced the expression of phosphatase and tensin homolog (PTEN), a vital tumor suppressor gene in hepatocellular carcinoma."
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"20 In tumor xenografts in athymic nude mice, inhibition of PTEN expression by injection of PTEN anti-sense oligonucleotides conferred resistance to trastuzumab."
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"We constructed a PTENα expressing oncolytic virus, HSV-P10, using a modified PTENα gene sequence, whereby we mutated the PTENα CUG start codon to AUG to enhance translation of the full-length N-terminally extended protein, and the internal canonical PTEN AUG start codon to AUA to abrogate canonical PTEN expression from the construct (Fig. 1a) ."
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"RESULTS Reporter assay indicated miR-152 targets the 3 ' UTR of PTEN mRNA to inhibit PTEN expression."
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"As expected, silencing of PTEN with RNAi in the PRL-3 low expression group restored the expression of PTEN and the Akt expression (XREF_FIG)."
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"XREF_BIBR, XREF_BIBR PTEN deficient melanocytes were found to be larger in mouse models, XREF_BIBR and reduced nuclear PTEN expression in human acquired melanocytic nevi has been established XREF_BIBR; we speculate that PTEN mutation may have played a role in iris mammillations development in our patients."
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"Deletion of TEP1 leads to greatly reduced vRNA levels and a loss of TEP1 and vRNA from the vault caps but no perturbation in telomerase activity or telomere length [182,183]."
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"None of our samples investigated showed intragenic LOH of the PTEN gene.Complete loss of PTEN expression was observed in only 1 out of 19 different tumors, epithelial and blastemal structures reacting[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"In addition HOTAIR represses the expression of phosphatase and tensin homolog (PTEN), an inhibitor of EMT, in laryngeal squamous cell carcinoma cells [XREF_BIBR]."
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"Moreover, the induction of PTEN attenuated the Akt phosphorylation levels, pretreatment of PI3K inhibitor LY294002 in NB4 cells, significantly augmented the cell differentiation and increased the expression of PTEN."
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"SMYD2-mediated methylation of PTEN at Lys313 was shown to downregulate the expression of PTEN and to suppress PTEN activity through inducing phosphorylation at serine 380 of PTEN[27,30]."
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"PTEN siRNA interference strongly downregulates expression of PTEN protein."
31500630
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"For the melanoma model, two-day-old Braf CA/+ Pten fl/fl Tyr Cre +/0 mice (designated BPT) were painted with 4-hydroxytamoxifen (H6278, Sigma-Aldrich, Darmstadt, Germany) on the right flank skin to activate BRAF 600E expression and inactivate PTEN expression in melanocytes, as described [XREF_BIBR]."
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"Overexpression of XIAP induces polyubiquitination of PTEN and proteasome dependent decrease of PTEN protein levels."
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"MiR-214 induces cell survival and cisplatin resistance through targeting the 3 '-UTR of the PTEN gene, which leads to reduced expression of PTEN and activation of the Akt pathway 92."
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"Although activation of PTEN drives Treg cell differentiation, diminished expression of PTEN is also detrimental for Th17 cell differentiation because it increases IL-2 production [XREF_BIBR]; this suggests that extreme PTEN levels (low or high) disrupt the Th17 and Treg balance."
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"Protein blot analysis confirmed that Pten hy/+ MMECs had decreased levels of Pten and increased levels of pAkt and cyclin D1 (XREF_FIG)."
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"The homozygous Pten (m3m4) mice have decreased total Pten levels including a specific drop in nuclear Pten and exhibit region specific increases in brain weight."
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"PTEN is mutated in a subset of late-stage ovarian cancer patient samples and decreased PTEN expression accompanies the progression of ovarian cancer [6]."
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"We therefore conclude that at least a subset of PHTS related PTEN nonsense or missense germline mutations have diminished PTEN protein levels and proteasome hyperactivity."
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"We therefore compared the extent of the magnitude of changes in expression of the PTEN signature genes induced by the PTEN deletion with the control diet to that when the diet was supplemented with SF."
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"PTEN was present in Xenopus spinal neurons, and we interfered with its activity in three separate ways : by applying the drug bpV, by using a PTEN specific MO to lower neuronal PTEN expression, and by expressing catalytically dead PTEN in neurons."
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"RD cells have PTEN promoter hypermethylation and display decreased protein expression of PTEN, compared to other RMS cells (36)."
36322002
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"The alpha isoform acts on the PTEN promoter inhibiting PTEN transcription by epigenetic mechanisms."
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"The PTEN protein negatively regulates the PI3K pathway XREF_BIBR and loss of PTEN protein expression has been linked to poor survival in patients with tongue cancer, and with more advanced tumor in esophageal and oral squamous cell cancers, respectively XREF_BIBR, XREF_BIBR."
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"For Dicer HCT116 cells, we found depletion of the putative PTEN ceRNAs resulted in higher PTEN protein levels (SERINC1: 290%; VAPA: 144%; CNOT6L: 256%) relative to control siRNA (average PTEN expression = 100%), while targeting PTEN reduced PTEN protein levels to an average of 2.6%."
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"Three PTEN siRNAs (siPTEN # 1, 2 and 3) and three SPRY1 siRNAs (siSPRY1 # 1, 2 and 3) were used to downregulate the expression of PTEN and SPRY1 in HSFs, respectively."
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"PTEN inhibited the BMP2-induced osteoblast differentiation of MSCs at the osteoblast lineage commitment level and at the early and late stages of osteogenic differentiation, while BMP2 repressed PTEN expression, leading to the activation of PI3K/AKT and to osteogenesis."
| PMC
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"Studies of the aberrant expression of several miRNAs in the B cells of SLE patients have reported that miR-7, miR-155, miR-30a, and miR-15a were up-regulated, while miR-1246 were down-regulated (Figure 3), and researches have preliminarily demonstrated their role in SLE.A recent study reported that he up-regulation of miR-7 in the B cells of SLE patients can negatively regulate the expression of phosphatase and tensin homolog (PTEN), which results in up-regulated activation of PI3K/AKT signaling (35, 36)."
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"We previously demonstrated that transcription of PTEN is negatively regulated by the PTEN pseudogene antisense RNA, PTENP1-AS, and here we investigated the impact of this transcript on clinical outcome and BRAFi resistance in melanoma."
34040017
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"Reporter assay indicated miR-152 targets the 3 ' UTR of PTEN mRNA to inhibit PTEN expression."
27840403
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"These data suggest that SA directly binds and inhibits the activity and that attenuated DNMT1 mediated PTEN hypermethylation caused the loss of PTEN expression, followed by the inhibition of the AKT and ERK pathways and prevented the development of liver fibrosis."
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"Myocyte specific Pten deficiency in mice decreased PTEN protein levels and increased Akt phosphorylation in the heart, suggesting inactivation of PTEN in cardiac muscle (XREF_FIG)."
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"Similarly, a hypoxic tumor microenvironment can induce oral squamous cell carcinoma cells (OSCCs) to generate more miR-21-containing exosomes that are then transferred to normoxic OSCCs to enhance their migration and invasion by inhibiting expression of phosphatase and tensin homolog (PTEN) and PDCD4."
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"Two animal genotypes were selected for injection : Pten hy /+ (" Pten hyper ") mice with 25% reduced Pten levels, to test if Myc cooperates with activation of Akt in driving metastasis, and WT mice, to test if Myc can drive metastasis on its own."
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"To elucidate the effect of PTEN on trastuzumab 's efficacy, SKBR3/WT, 4R and 8R cells were transfected with PTEN antisense oligonucleotides, which effectively reduced endogenous PTEN expression compared with the cells transfected with control mismatched (MIS) oligonucleotides (XREF_FIG)."
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"This suggests that posttranscriptional regulation of PTEN may decrease PTEN protein expression and be associated with adverse outcomes in patients with no allelic loss of PTEN."
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"In this study, we have identified a novel mechanism in which RBM38 enhanced the PTEN transcript by binding directly the AREs in the 3 '-UTR of PTEN mRNA to suppress PTEN protein expression."
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"Garofalo et al. [XREF_BIBR] showed that miR-221 and miR-222, which are upregulated in NSCLC cells and hepatocellular carcinomas, target a site in the PTEN 3 '-UTR that causes reduced PTEN expression and increased resistance to the TNF related apoptosis inducing ligand (TRAIL)."
26501795
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"Additionally, BC cells can take up miR19a-enriched exosomes derived from astrocytes to reduce the expression of phosphatase and tensin homolog (PTEN), a major tumor suppressor."
34638283
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"Our data suggest that stimulation through CD28 is required in order to up-regulate miR-214 which in turn targets the 3 ' UTR of Pten thereby reducing PTEN levels and promoting T cell activation."
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"In vitro, the loss of PTEN in epithelial cells induces senescence [XREF_BIBR] and overexpression of PTEN reverses this effect [XREF_BIBR]."
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"In these cells, a pool of inhibitors of endogenously expressed PTEN targeting microRNAs (XREF_SUPPLEMENTARY) de-repressed both PTEN and PTENP1 transcript levels (XREF_FIG)."
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"Repression of PTEN expression by PTEN siRNAs or SF1670 (PTEN inhibitor) rescued the tumor inhibiting effect of DOK7 overexpression, suggesting that DOK7 inhibits proliferation, migration, and invasion of breast cancer cells though the PI3K/PTEN/AKT pathway."
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"Prostatic Pten expression was indeed about half on the Pten +/- background, and was repressed by ETV1 (or castration) in prostates of Pten +/+ but not Pten +/- mice (XREF_FIG)."
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"Transfection of PTEN shRNA into OV2008 cells remarkably down-regulated expression of PTEN and up-regulated expression of phospho-AKT protein, with transfected cells being resistant to cisplatin."
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"As expected, infection of Pten loxP and loxP MEFs with Adeno-Cre reduces PTEN expression, and elevates both Akt S473 and Akt T308 phosphorylation in serum deprived cells [XREF_FIG, lanes 1 & 3] and in insulin stimulated cells [XREF_FIG, lanes 2 & 4]."
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"XREF_BIBR PTEN promoter methylation may have decreased the expression of PTEN, XREF_BIBR since expression of the PTEN gene was associated with low methylation levels."
26730203
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"Based on our observations that the Pten m3m4 and m3m4 mutant mice have diminished Pten protein levels in the brain [XREF_BIBR], we hypothesized that ASD associated PTEN mutations decrease the stability of PTEN protein."
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"50 In addition, two groups of authors demonstrated that the loss of PTEN resulted in the activation of the PI3K/AKT/mTOR pathway.31 32 To clarify the underlying mechanism mediated by circRAB31/miR-885-5p/PTEN axis, we performed western blot and found that PTEN induced by circRAB31 overexpression decreased the protein level of p-AKT, while silencing of circRAB31 inhibited the PTEN protein level and promoted the p-AKT protein level."
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"Under these conditions, two of four PTEN shRNAs reduced PTEN protein levels in the transformed MEF cells (XREF_FIG) while simultaneously enhancing cell survival (XREF_FIG) and proliferation as assessed by BrdU incorporation (XREF_SUPPLEMENTARY) without affecting apoptosis (XREF_SUPPLEMENTARY)."
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"PTEN antisense delivered SKBR3/WT cells, which had reduced PTEN expression, showed significantly less growth inhibition with trastuzumab than MIS control delivered cells with a normal level of PTEN expression (XREF_FIG)."
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"In contrast, knockdown of PTEN expression by stable transfection of PTEN small interfering RNA greatly decreased the apoptosis rate."
20089040
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"PTEN suppressed the expression of PTEN not only in GCL but also in INL, which was in accord with the transduction of RGCs and Muller cells."
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"On the contrary, depletion of PTEN reduced the expression of both lnc-2 and lnc-6,and the reduction of PTEN expression by overexpressing known PTEN-regulating miRNAs could be rescued by overexpressing lnc-2 sub-sequences [126]."
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