IndraLab

"Among them, G3BP1/2 is regulated by the phosphorylation site of S149 and may bind to caprin1 or USP10; binding to caprin1 may promote the formation of SGs, while binding to USP10 may inhibit the formation of SGs (23) ."

"This FGDF motif is also found in SFV and USP10, which can also bind to G3BP1/2 to block the assembly of SGs (82)."

"We now show that (a) deletion of G3BP1/2 ablates SGs triggered by p-eIF2alpha or eIF4A inhibition, but not those induced by hyperosmolarity or severe heat shock, (b) the phosphomimetic G3BP1-S149E fails to rescue SG competence, (c) binding of G3BP to Caprin1 or USP10 is not required for SG competence, but may alter G3BP and USP10 protein levels, (d) Caprin1 and USP10 bind G3BP1/2 in a mutually exclusive manner with antagonistic effects on SGs, (e) G3BP1 interacts with 40S ribosomal subunits through its RGG motif, which is required for SG competence, (f) overexpression of USP10 inhibits SG formation downstream of polysome disassembly, and (g) USP10 and FGDF binding to G3BP specifically alters the B-isox precipitation of G3BP1/2 and Caprin1, but not that of other SG associated proteins."