IndraLab
Statements
isi
"Furthermore, knockdown of CIgG upregulated PTP-BAS mRNA and protein expression, whereas cisplatin downregulated PTP-BAS protein, but not mRNA expression; knockdown of PTP-BAS upregulated phosphorylation of Src, PDK1, AKT, and blocked CIgG knockdown-mediated enhancement of cisplatin-induced inhibition of cell proliferation."
reach
"As shown in Figure 2B‐E, individual treatments of erlotinib, AG490, LY294002 and PD98059 all led to a substantial decrease in YD‐38 cell survival.3.3
Knockdown of Src causes a strong inhibition of the phosphorylation of EGFR, STAT-5, PKB and ERK-1/2 in YD-38 human oral cancer cells."
sparser
"Further, FAK and CDCP1 tyrosine phosphorylation were recently shown to be reciprocally related, xref , xref and CDCP1 cleavage-dependent increases in CDCP1 tyrosine phosphorylation protect cells from anoikis through mechanisms involving enhanced Src and PKCδ tyrosine phosphorylation and activation of the pro-survival kinase Akt. xref This latter study demonstrated that plasmin is an important regulator of CDCP1 cleavage in vivo ."
reach
"In human tracheal smooth muscle cells, TNF-alpha induced MMP-9 expression and Akt phosphorylation in a time dependent manner, which was attenuated by the inhibitors of Src (PP1), epidermal growth factor receptor (AG1478), PDGFR (AG1296), and PI3K (LY294002), respectively, revealed by reporter gene assay, RT-PCR, zymographic, and Western blot analyses."
reach
"Because of the importance of receptor and nonreceptor tyrosine kinases in G protein coupled receptor signaling, we studied the role of epidermal growth factor receptor (EGFR), c-Src, and phosphoinositide 3-kinase (PI3K) on OT induced PGF (2 alpha) production in association with cyclooxygenase 2 (COX2) expression and ERK1/2 and Akt phosphorylation."
sparser
"The recruitment of β-arrestin to the AT1R possibly engages a pathway where Src phosphorylates and activates Akt, which in turn phosphorylates endothelial nitric oxide synthase (eNOS) ( xref ; xref ), which could further provide a connection between AT1R-arrestin function and cardiovascular tone via nitric oxide regulation ( xref )."
sparser
"The starting point is the
G i/o -protein dependent PI3K activation that leads to the membrane
recruitment of Akt, which becomes tyrosine phosphorylated by c-Src with the
subsequent phosphorylation in both the activation loop within the kinase domain
[A-loop (T308)] and the hydrophobic motif in the C-terminal region [HM (S473)] by
PDK1 and mTORC2, respectively."
sparser
"Although we cannot exclude the possibility that eHsp90 influences LRP1 endocytic function, our data support a model whereby eHsp90-LRP1 functions as a signal transduction complex to regulate src dependent AKT phosphorylation, resulting in P-EphA2 S897 directed cell motility and invasion."
"Caov-3 cells treated with 17 beta-estradiol (E2) when treated with anti-phospho-Akt antibody revealed no change in Akt expression, but induced transient phosphorylation of Akt. Addition of PP2, a Src inhibitor resulted in attenuation of E2 induced Akt phosphorylation, suggesting that E2 induced Akt phosphorylation through ER and Src."
reach
"One possibility is that Src may regulate this pathway through activation of AKT since Src can directly induce AKT phosphorylation and AKT can induce phosphorylation of ubiquitin specific protease 4 (USP4), a deubiquitinating enzyme that prevents TGF-beta receptor I degradation [XREF_BIBR]."
reach
"We found that the levels of phosphorylated (p-) AKT, mTOR, Bad, Src, FAK, and paxillin were higher in the stable CHIP knockdown Panc-1 and BxPC-3 cells, and the levels of p-AKT, p-mTOR, p-Bad, p-Src, p-FAK, and p-paxillin significantly decreased in CHIP OE cells, while the total protein did not change in the CHIP knockdown and CHIP OE cells."
reach
"Together, these data indicate that CDCP1 mediated survival signaling in vivo involves Src dependent phosphorylation of 70 kDa CDCP1, Akt activation, and suppression of PARP1 cleavage, all of which are linked directly to the proteolysis induced cleavage of CDCP1 expressed on the surface of bona fide carcinoma cells or transformed cells."
reach
"The dramatic phosphorylation of Akt and to a lesser extent MAPK and Src, in response to SDF-1alpha in duct cells of the IFNgammaNOD transgenic mice, indicate a potential involvement of these signaling proteins in the regulation of migration, proliferation, and survival of the pancreatic progenitor population."
rlimsp
"We found that (i) ammonia increases EGF receptor phosphorylation at EGFR(845) and EGFR(1068); (ii) ammonia-induced ERK1/2 and AKT phosphorylation depends on the activity of EGF receptor and Src, but not on metalloproteinase; (iii) AKT phosphorylation occurs upstream of ERK1/2 phosphorylation; (iv) ammonia stimulates association between the α1 Na,K-ATPase isoform, Src, EGF receptor, ERK1/2, AKT and caveolin-1; (v) ammonia-induced ROS production might occur later than EGFR transactivation; (vi) both ammonia induced ERK phosphorylation and ROS production can be abolished by canrenone, an inhibitor of ouabain, and (vii) ammonia-induced cell swelling depends on signaling via the Na,K-ATPase/ouabain/Src/EGF receptor/PI3K-AKT/ERK1/2, but in response to 3mM ammonia it does not appear until after 12h."
reach
"We thus took advantage of specific Src kinase activity inhibitor PP1 and SU6656 to investigate the effects of Src kinase on EGFR transactivation and subsequent Akt activation, and found that Src kinase inhibition blocked PTHrP (1–34)-induced EGFR and Akt phosphorylation (Figure 2D)."