IndraLab

Statements

RIPK1 binds CYLD. 39 / 42
4 | 16 19
sparser
"Close proximity of binding sites of both CYLD and RIP on optineurin provides support for the adaptor function of optineurin in facilitating interaction of RIP and CYLD."
21408173
reach
"The interaction of Flag-CYLD and Myc-RIP1 was taken as positive control."
33654169
sparser
"These results show that optineurin is essential for interaction of CYLD with ubiquitinated RIP."
21408173
sparser
"Moreover, siCLIPR-59 treatment disrupted the formation of protein complex of CYLD with RIP1 ( xref )."
22297296
reach
"In contrast, under TNFalpha treatment, binding between CYLD and RIP1 or TRAF2 was reduced, if the cells also expressed PrP (XREF_FIG B)."
28900035
reach
"The interaction of CYLD and RIP1 (Receptor Interaction protein 1) was taken as positive control."
33654169
sparser
"In contrast, in siCLIPR-59-treated cells, the association of CYLD with RIP1 was reduced ( xref )."
22297296
sparser
"Optineurin is essential for interaction of CYLD with ubiquitinated RIP."
21408173
reach
"Mediating the interaction between CYLD and polyUb RIP, optineurin may act as an adaptor protein bringing CYLD and the CYLD substrate RIP together to facilitate deubiquitination of ubiquitinated RIP by CYLD."
22364875
sparser
"The results presented in this manuscript suggest that optineurin mediates interaction of deubiquitinase CYLD with polyubiquitinated RIP and this interaction is essential for deubiquitination of RIP by CYLD."
21408173
sparser
"CYLD binds to RIP1 and inhibits its ubiquitination and signaling function."
17981138
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"32 Last but not least, CYLD interacts with and deubiquitinates RIP1."
27383048
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"Because Lys-63-ubiquitylation of receptor interacting protein 1 (RIP1) is a vital element in NF-kappaB signaling, CYLD interacts withand deubiquitylates RIP1 in wild-type germ cells and blocks the abe[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
20347313
sparser
"These co-IP assays provided evidence that CLIPR-59 is needed for the formation of protein complex containing CYLD and RIP1."
22297296
reach
"Interactions between CYLD and RIP1 or TRAF2 were specific as isotype controls did not pull down any CYLD (XREF_FIG, A and B)."
28900035
reach
"CLIPR-59, an adaptor protein, facilitates the interaction between CYLD and RIPK1."
25459879
reach
"We find that CYLD can interact with RIP1 in HEK 293 cells upon co-transfection (XREF_SUPPLEMENTARY) and that CYLD is an efficient deubiquitinase for RIP1 (XREF_SUPPLEMENTARY)."
22848449
reach
"Close proximity of binding sites of both CYLD and RIP on optineurin provides support for the adaptor function of optineurin in facilitating interaction of RIP and CYLD."
21408173
sparser
"CLIPR-59, an adaptor protein, facilitates the interaction between CYLD and RIPK1 ( xref )."
25459879
sparser
"The interaction of CYLD and RIP1 (Receptor Interaction protein 1) was taken as positive control."
33654169
sparser
"The main feature of this model is that optineurin mediates binding of CYLD to ubiquitinated RIP."
21408173
sparser
"This interaction mediates a subsequent interaction between CYLD and RIP ( xref ), the latter acting as an adaptor upon its ubiquitination of NEMO, which senses the polyubiquitination of RIP and activates downstream NF-κB signalling via IκB kinase complex ( xref , xref )."
29867991
reach
"However, this interaction is likely to be indirect, as expression of CYLD in 293T cells or in baculovirus did not reveal a strong interaction between CYLD and RIP1 (data not shown)."
24098568
reach
"Thus, in the presence of PrP, TNFalpha treatment increased the interaction between PrP and CYLD but alleviated binding between CYLD and RIP1 or TRAF2."
28900035
reach
"TNFalpha treatment increases the binding between PrP and the deubiquitinase tumor suppressor cylindromatosis (CYLD), in these treated cells, binding of CYLD to RIP1 and TRAF2 is reduced."
28900035
reach
"Optineurin may be a ubiquitin binding adaptor for CYLD in the TNFR pathway, since knockdown of optineurin impaired CYLD binding to RIP1 and NF-kappaB inhibition in TNF stimulated cells."
22435550
sparser
"Adaptors such as the recently reported CLIPR-59 may mediate the interaction between RIP1 and CYLD [ xref ]."
24098568
biogrid
No evidence text available
22848449
sparser
"We find that CYLD can interact with RIP1 in HEK 293 cells upon co-transfection ( xref ) and that CYLD is an efficient deubiquitinase for RIP1 ( xref )."
22848449
reach
"This early checkpoint revolves around an interaction between the deubiquitinase CYLD and its target RIPK1."
32024820
sparser
"However, this interaction is likely to be indirect, as expression of CYLD in 293T cells or in baculovirus did not reveal a strong interaction between CYLD and RIP1 (data not shown)."
24098568
biogrid
No evidence text available
17981138
biogrid
No evidence text available
21408173
sparser
"These assumptions are strengthened by our observations which show that both inhibition of NF-κB activation by CYLD and association of CYLD with RIP are dependent on optineurin."
21408173
reach
"CYLD binds to RIP1 and inhibits its ubiquitination and signaling function."
17981138
biogrid
No evidence text available
28900035
sparser
"To clarify the role of CLIPR-59 in the formation of RIP1 and CYLD-containing protein complex, we assessed the effect of siCLIPR-59 on the association of CYLD with RIP1 in TNF- α -stimulated cells by co-IP assays using anti-RIP1-specific Ab."
22297296
sparser
"Since CYLD failed to deubiquitinate RIP in the absence of optineurin, it is likely that optineurin acts as an adaptor to facilitate interaction of CYLD with RIP."
21408173
sparser
"This early checkpoint revolves around an interaction between the deubiquitinase CYLD and its target RIPK1."
32024820