IndraLab

Statements

Pfo binds KCNMA1. 12 / 12
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sparser
"PFO and SLO toxins both bind to cholesterol, and their oligomerisation can be modulated by cholesterol concentration in the target membrane [ xref ]."
35333704
sparser
"SLO and PFO bind to cholesterol on the host membrane, oligomerize, and form pores in host lipid membranes ( xref )."
35294243
reach
"We conclude that sphingolipids comprise one key component of the non-permissive membrane environment in Leishmania that reduces CDC pore-formation.We used differences in SLO and PFO binding to mechanistically probe the non-permissive environment."
37094699
sparser
"Some toxins showed highly specific binding; for example, LLY bound a single structure, while other CDCs such as SLO and PFO bound to a broad range of glycans."
32494740
sparser
"Furthermore, cholesterol depletion of erythrocytes did not greatly affect binding of PFO, SLO and ILY to membranes, but rather locked these CDCs in the prepore complex [33] ."
17720603
sparser
"Surprisingly, the sphingolipid-null spt2 — ( xref ) showed no increase in SLO or PFO ML binding ( xref , A and B )."
37094699
sparser
"We used differences in SLO and PFO binding to mechanistically probe the non-permissive environment."
37094699
reach
"Furthermore, cholesterol depletion of erythrocytes did not greatly affect binding of PFO, SLO and ILY to membranes, but rather locked these CDCs in the prepore complex [33] ."
17720603
reach
"To test if the differences in membrane binding between PFO and SLO change the rate at which these PFTs kill nucleated cells, we challenged cells with equivalent hemolytic doses of PFO or SLO for 5 to 30 min and measured cytotoxicity by flow cytometry using propidium iodide (PI) uptake (XREF_FIG and Supplemental Figure S1)."
30577571
sparser
"PFO and SLO both bind to cholesterol-rich membranes and the α-carbons of the membrane binding loops in the crystal structures of PFO and SLO overlap [ xref , xref ]."
30577571
sparser
"Both PFO and SLO form variably sized pores of 20–30 nm [ xref , xref , xref ], suggesting that pore size does not account for changes in cytotoxicity."
30577571
reach
"A threonine leucine pair conserved in all CDCs and located in L1, mediates direct binding of PFO, SLO (streptolysin O, secreted by Streptococcus pyogenes), and PLY (pneumolysin, produced by Streptococcus pneumoniae) to cholesterol [XREF_BIBR]."
24798012