IndraLab

Statements

KCNMA1 binds pfo. 7 / 7
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"A threonine leucine pair conserved in all CDCs and located in L1, mediates direct binding of PFO, SLO (streptolysin O, secreted by Streptococcus pyogenes), and PLY (pneumolysin, produced by Streptococcus pneumoniae) to cholesterol [XREF_BIBR]."
24798012
sparser
"PFO and SLO both bind to cholesterol-rich membranes and the α-carbons of the membrane binding loops in the crystal structures of PFO and SLO overlap [ xref , xref ]."
30577571
reach
"To test if the differences in membrane binding between PFO and SLO change the rate at which these PFTs kill nucleated cells, we challenged cells with equivalent hemolytic doses of PFO or SLO for 5 to 30 min and measured cytotoxicity by flow cytometry using propidium iodide (PI) uptake (XREF_FIG and Supplemental Figure S1)."
30577571
sparser
"SLO and PFO bind to cholesterol on the host membrane, oligomerize, and form pores in host lipid membranes ( xref )."
35294243
sparser
"Some toxins showed highly specific binding; for example, LLY bound a single structure, while other CDCs such as SLO and PFO bound to a broad range of glycans."
32494740
sparser
"Both PFO and SLO form variably sized pores of 20–30 nm [ xref , xref , xref ], suggesting that pore size does not account for changes in cytotoxicity."
30577571
sparser
"PFO and SLO toxins both bind to cholesterol, and their oligomerisation can be modulated by cholesterol concentration in the target membrane [ xref ]."
35333704