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AKT phosphorylates NOS3 on S1179. 98 / 99
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"Activated SHIP-1 attenuates signaling downstream of PI3 kinase, blunting Akt activation and eNOS Ser1179 phosphorylation by Akt, and thereby decreasing eNOS enzyme activation. xref , xref However, how CRP activates FcγRIIB is not known."
21940940
sparser
"Studies showed that Ser 1179 of eNOS is phosphorylated by protein kinase Akt, which increases electron flux through the reductase domain and NO production."
21559738
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"Akt is downstream from PI 3-kinase and is capable of directly phosphorylating eNOS at Ser 1179 , resulting in its activation [38] ."
21439968
rlimsp
"Extensive literature indicates that phosphorylation by Akt on Ser1179 of eNOS leads to augmentation of NO production [5, 17]."
31781259
rlimsp
"Besides acting as an allosteric enhancer, Hsp90 was shown to serve as a module recruiting Akt to phosphorylate the serine 1179/1177 (bovine/human) residue of eNOS."
15737995
reach
"Both vascular endothelial growth factor and FSS activate endothelial protein kinase B (PKB) by way of incompletely understood pathway (s), and, in turn, PKB phosphorylates eNOS at Ser 1179, causing its activation."
11287317
reach
"Moreover, administration of endothelial cells with VEGF induced AKT that can phosphorylate eNOS on Ser 1179."
36731150
reach
"Along this line, the increase in Akt in response to S1P induced the phosphorylation of eNOS at serine 1179, which first hydroxylated L-arginine to Nω-hydroxy-L-arginine and then oxidized Nω-hydroxy-L-arginine to L-citrulline and NO [184,185]."
36671005
reach
"HDL stimulation of eNOS entails sequential activation of Src kinase (s), PI3 kinase, Akt kinase and Erk1/2 MAPK, with Akt phosphorylation of Ser1179 of eNOS causing enzyme activation 6."
23023567
reach
"Akt can phosphorylate eNOS on Ser 1179, and that phosphorylation enhances the enzyme 's ability to generate NO."
18078008
reach
"Because AMPK and Akt can phosphorylate and activate eNOS at Ser1179 in endothelial cells, XREF_BIBR - XREF_BIBR eNOS phosphorylation was examined in these cultures."
24052499
reach
"During ischemia, 15 min prior to reperfusion, mice were given geldanamycin, an inhibitor of hsp90 recruitment to eNOS, or LY-294002 an inhibitor of phosphatidylinositol 3-kinase (PI3K), an enzyme that initiates Akt catalysed phosphorylation of eNOS on Ser1179."
17450173
reach
"Akt inhibition prevented eNOS Ser1179 phosphorylation, whereas inhibition of PKA did not influence Akt and eNOS Ser1179 phosphorylation."
14645258
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"Phosphorylation of eNOS serine 1179 mediated by Akt has been shown to boost its activity ( Fulton et al., 1999; Wu, 2002 ) and, hypoxia-related stimuli, such as VEGF and shear stress, phosphorylate an[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17976938
reach
"For example, eNOS phosphorylation at Ser 1179 by Akt increases NO production [7], while basal phosphorylation of eNOS at Thr 495 reduced its catalytic activity [21]."
24769202
sparser
"Akt-dependent phosphorylation of eNOS at Ser1179 is critical for endothelium-dependent relaxation [ xref ], while phosphorylation at Thr497 is considered inhibitory [ xref ]."
24720460
sparser
"At certain conditions, such as VEGF stimulation, AKT binds with Hsp90 (327-340aa) and phosphorylates eNOS Serine 1179[ xref , xref ]."
28654706
sparser
"Akt kinase phosphorylates eNOS at Ser1179, and is likely to be an important in vivo regulator of eNOS activity [33,34] ."
15261488
reach
"The molecular mechanism of eNOS activation by apigenin involves continuous eNOS Ser1179 phosphorylation by the PI3K and Akt pathways [XREF_BIBR]."
32326376
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"The serine/threonine kinase Akt (protein kinase B) directly phosphorylates eNOS at Ser1177 (human eNOS) and Ser1179 (bovine eNOS), which increases eNOS activity several-fold [12,13,31]."
12559774
reach
"Protein kinase Akt can directly activate eNOS by phosphorylating eNOS on serine 1179 (1177), which leads to NO generation [35,36] ."
28870504
rlimsp
"Akt can phosphorylate eNOS on Ser 1179, and that phosphorylation enhances the enzyme’s ability to generate NO (Fulton et al 1999)."
18078008
reach
"In addition, Akt-mediated expression and phosphorylation of eNOS at Ser1179 is reduced thereby, impairing endothelial cell-driven NO-related effects on vascular tone and homeostasis [20] ."
36306821
reach
"Akt also phosphorylates eNOS at Ser-1179, enhancing the production of NO as another mechanism to inhibit apoptosis as described in Section 2.1.4 (Cirino et al., 2003) ."
15031001
rlimsp
"Furthermore, it has been reported that eNOS is activated by direct phosphorylation of serine 1179 (Ser1179) by Akt, a downstream target of phosphatidylinositol 3-kinase [7]."
27610041
reach
"Phosphorylation of eNOS at Ser 1179 is stimulated by Akt kinase or protein kinase A and results in increased enzyme activity leading to an enhanced NO production, thus allowing to couple extracellular[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16270749
sparser
"The activity of eNOS is reportedly increased not only by translocation of eNOS from cellular membrane to cytosol, but also by Akt-dependent phosphorylation of eNOS at Ser 1179 ( Dimmeler et al., 1999;[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
22728282
reach
"Inhibition of PI3K/Akt and MEK/ERK1/2 prevented phosphorylation of eNOS at Ser1179 by the inhibitors wortmannin and PD98059 ( Fig. 3A ) or by transfection of the dominant-negative Akt cDNA ( Fig. 4 ),[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15358561
rlimsp
"Because AMPK and Akt can phosphorylate and activate eNOS at Ser1179 in endothelial cells, eNOS phosphorylation was examined in these cultures."
24052499
reach
"Akt kinase phosphorylates the eNOS at serine 1179 while increasing NO production [61]."
30030121
sparser
"ENOS is reported to be activated by Akt-dependent phosphorylation of eNOS at Ser 1179 [95] to exert the properties of antiatherosclerosis, including inhibition of smooth muscle cell proliferation, pla[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
25956424
reach
"Insulin promotes the protective cardiovascular endothelial functions by activating Akt, which then phosphorylates endothelial NO synthase (eNOS) in Ser1179, stimulating the production of cardioprotective NO [162]."
35563860
reach
"Moreover, phosphorylation of human eNOS by Akt at Ser 1179 and subsequent release of NO occur at sub-physiological levels of calcium in endothelial cells [28,29] , suggesting that phosphorylation of e[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
24407281
phosphoelm
No evidence text available
12501214
reach
"The serine/threonine protein kinase Akt can directly phosphorylate eNOS on serine 1179 and activate the enzyme, leading to NO production."
24518819
reach
"The phosphorylation of eNOS at Ser 1179, and not Thr 495, is mediated by Ca 2+ and the PI3K and Akt pathway [XREF_BIBR]."
20423726
rlimsp
"Insulin-stimulated activation of eNOS is independent of Ca2+ but requires phosphorylation by Akt at Ser(1179). Vasodilator actions of insulin are mediated by activation of endothelial nitric-oxide synthase (eNOS) and subsequent production of NO. Phosphatidylinositol 3-kinase and Akt play important roles in insulin-signaling pathways leading to production of NO in vascular endothelium. Here we dissected mechanisms whereby insulin activates eNOS by using the fluorescent dye DAF-2 to directly measure NO production in single cells. Insulin caused a rapid increase in intracellular NO in NIH-3T3(IR) cells transiently transfected with eNOS. The stimulation of NO production by lysophosphatidic acid (LPA) was abrogated by pretreatment of cells with the calcium chelator 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid. Remarkably, in the same cells, insulin-stimulated production of NO was unaffected. However, cells expressing the eNOS-S1179A mutant (disrupted Akt phosphorylation site) did not produce detectable NO in response to insulin, whereas the response to LPA was similar to that observed in cells expressing wild-type eNOS. Moreover, production of NO in response to insulin was blocked by coexpression of an inhibitory mutant of Akt, whereas the response to LPA was unaffected. Phosphorylation of eNOS at Ser(1179) was observed only in response to treatment with insulin, but not with LPA."
11402048
reach
"Here we show that the serine/threonine protein kinase Akt (protein kinase B) can directly phosphorylate eNOS on serine 1179 and activate the enzyme, leading to NO production, whereas mutant eNOS (S1179A) is resistant to phosphorylation and activation by Akt."
10376602
sparser
"Akt kinase phosphorylates the eNOS at serine-1179 while increasing NO production [ 61 ]."
30030121
reach
"Several groups independently reported the important findings that the serine/threonine protein kinase Akt (protein kinase B) directly phosphorylates eNOS on Ser1179 and activates the enzyme, leading t[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
11551775
reach
"Full activation of eNOS by S1P requires a pathway comprising G-protein-coupled S1P receptors; pertussis toxin-sensitive G-proteins, especially G-protein beta-gamma subunits that modulate the beta-isof[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18638569
reach
"At certain conditions, such as VEGF stimulation, AKT binds with Hsp90 (327-340aa) and phosphorylates eNOS Serine 1179 [XREF_BIBR, XREF_BIBR]."
28654706
reach
"In fact, recent evidence suggests that the phosphorylation of eNOS at Ser 1179 (in the bovine eNOS sequence; equivalent to human eNOS-S 1177 ) by a sequential activation of the phosphoinositide-3-kina[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15186944
reach
"Phosphorylation of eNOS at both Ser1177 and Ser633 in human endothelial cells (Ser1179 and Ser635 in bovine endothelial cells) by Akt, calmodulin-dependent protein kinase II and AMP-activated protein kinase increases eNOS activity [34,42]."
31972507
sparser
"Several groups independently reported the important findings that the serine/threonine protein kinase Akt (protein kinase B) directly phosphorylates eNOS on Ser1179 and activates the enzyme, leading t[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
11551775
reach
"Ser1177 (human eNOS) and Ser1179 (bovine eNOS) are phosphorylated by the serine/threonine kinase Akt (protein kinase B) [6,22,23] ."
20363286
reach
"The resulting increased Akt activation leads to enhanced eNOS phosphorylation at Ser 1177 and/or Ser 1179, thereby increasing the NO synthesizing activity."
30050658
rlimsp
"Of note, a simultaneous decrease was also observed in the active (phosphorylated) form of Akt (Thr308/Ser473), which has been demonstrated to phosphorylate eNOS at Ser1179."
24085225
reach
"Ser1179 phosphorylation of eNOS by the phosphatidylinositol 3-kinase-dependent Akt stimulates its catalytic activity."
17210830
sparser
"Insulin promotes the cardiovascular protective functions of the endothelium including NO production by endothelial NO synthase (eNOS), which it stimulates via Akt kinase which phosphorylates eNOS Ser1179."
19423845
sparser
"Ser1179 phosphorylation of eNOS by the phosphatidylinositol 3-kinase-dependent Akt stimulates its catalytic activity."
17210830
rlimsp
"Moreover, ghrelin stimulated increased phosphorylation of Akt (Ser473) and eNOS (Akt phosphorylation site Ser1179) that was inhibitable by knockdown of GHSR-1a using siRNA or by pretreatment of cells with wortmannin but not with PD-98059."
17106060
reach
"It was reported previously that VEGF stimulated phosphorylation of eNOS Ser 1179 by Akt activation, which was required by the formation of the eNOS and Hsp90 heterocomplex [15,16]."
22001744
sparser
"Both vascular endothelial growth factor and FSS activate endothelial protein kinase B (PKB) by way of incompletely understood pathway(s), and, in turn, PKB phosphorylates eNOS at Ser-1179, causing its activation."
11287317
sparser
"The serine/threonine kinase Akt (protein kinase B) directly phosphorylates eNOS at Ser1177 (human eNOS) and Ser1179 (bovine eNOS), which increases eNOS activity several-fold [12,13,31] ."
12559774
reach
"Akt dependent phosphorylation of eNOS at Ser1179 is critical for endothelium dependent relaxation [XREF_BIBR], while phosphorylation at Thr497 is considered inhibitory [XREF_BIBR]."
24720460
reach
"XREF_BIBR, XREF_BIBR Akt dependent phosphorylation of eNOS at Ser1179 is essential for endothelium dependent relaxation, 36 whereas phosphorylation at Thr497 is considered inhibitory."
22785174
sparser
"HDL stimulation of eNOS entails sequential activation of Src kinase(s), PI3 kinase, Akt kinase and Erk1/2 MAPK, with Akt phosphorylation of Ser1179 of eNOS causing enzyme activation xref ."
23023567
sparser
"In endothelial cells, eNOS can also be activated by Akt-dependent phosphorylation of eNOS at Ser 1179 ( Dimmeler et al., 1999; Fleming et al., 2001 )."
22728282
rlimsp
"Moreover, Akt-dependent phosphorylation of eNOS at Ser1179 has been proved to be essential for endothelium-dependent relaxation [40]."
31805910
rlimsp
"The serine/ threonine protein kinase Akt has been shown to increase human eNOS activity by Akt phosphorylation at Ser1179 and then lead to increase NO production, whereas mutant eNOS (Ser 1179A and Ser 1177) is resistant to phosphorylation and Akt-dependent activation [37, 38]. Reduced phosphorylation of eNOS at Ser1179 has previously been reported in diabetic rats [39]."
31805910
reach
"Taken together, these findings raise the possibility that ceramide may activate eNOS not only by stimulating its dissociation from caveolin-1, but also by increasing eNOS phosphorylation on serine 117[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15642349
sparser
"Full activation of eNOS by S1P requires a pathway comprising G-protein-coupled S1P receptors; pertussis toxin-sensitive G-proteins, especially G-protein beta-gamma subunits that modulate the beta-isof[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18638569
rlimsp
"The wild-type eNOS underwent agonist-induced phosphorylation at serine 1179 (a putative site for phosphorylation by kinase Akt), but phosphorylation of the myr(-) eNOS at this residue was nearly abrogated; the palm(-) eNOS exhibited an intermediate phenotype."
12189156
sparser
"Here we show that the serine/threonine protein kinase Akt (protein kinase B) can directly phosphorylate eNOS on serine 1179 and activate the enzyme, leading to NO production, whereas mutant eNOS (S1179A) is resistant to phosphorylation and activation by Akt."
10376602
sparser
"Akt also phosphorylates eNOS at Ser-1179, enhancing the production of NO as another mechanism to inhibit apoptosis as described in Section 2.1.4 (Cirino et al., 2003) ."
15031001
sparser
"The serine/threonine protein kinase Akt can directly phosphorylate eNOS on serine 1179 and activate the enzyme, leading to NO production ( xref )."
24518819
rlimsp
"Akt can directly phosphorylate eNOS on serine 1179 (based on the bovine eNOS sequence and equivalent to human eNOS-serine 1177) and activate the enzyme, leading to NO production. [38] Studies have shown that a crosstalk between RhoA–ROCK and Akt regulates eNOS phosphorylation independent of the RhoA/ROCK actions on the downregulation of eNOS expression."
21264179
rlimsp
"Akt can directly phosphorylate eNOS on serine 1179 (based on the bovine eNOS sequence and equivalent to human eNOS-serine 1177) and activate the enzyme, leading to NO production."
21264179
reach
"We now report that LPA treatment of bovine aortic endothelial cells (BAEC) activates eNOS enzyme activity in a pathway that involves phosphorylation of eNOS on serine 1179 by protein kinase Akt."
11939794
rlimsp
"LY294002, an inhibitor of phosphatidylinositol 3-kinase that initiates Akt-catalysed phosphorylation of eNOS on Ser1179, was administered 1 h before the induction of SCI; 24 h after SCI sections were taken for histological examination and for biochemical studies."
21496122
reach
"In endothelial cells, one possible mechanism for the enhanced NO production is an PI3 kinase and PKB/Akt-mediated stimulation of eNOS possibly by synergizing calcium-dependent eNOS phosphorylation at [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
19549592
sparser
"Moreover, Akt-dependent phosphorylation of eNOS at Ser1179 has been proved to be essential for endothelium-dependent relaxation [ xref ]."
31805910
rlimsp
"During ischemia, 15 min prior to reperfusion, mice were given geldanamycin, an inhibitor of hsp90 recruitment to eNOS, or LY-294002 an inhibitor of phosphatidylinositol 3-kinase (PI3K), an enzyme that initiates Akt-catalysed phosphorylation of eNOS on Ser1179."
17450173
rlimsp
"S1P treatment of BAEC leads to eNOS phosphorylation at Ser(1179), a residue phosphorylated by Akt; an eNOS mutant in which this Akt phosphorylation site is inactivated shows attenuated S1P-induced eNOS activation."
11278407
rlimsp
"However, ONOO(-) strongly inhibited the phosphorylation and activity of Akt, which is known to phosphorylate eNOS-Ser(1179)."
12107173
reach
"It has been reported that VEGFA activated PI3K and AKT directly phosphorylates eNOS Ser1179, leading to stimulating NO production, and endothelial migration, proliferation, and capillary like structure formation."
22106098
sparser
"Extensive literature indicates that phosphorylation by Akt on Ser1179 of eNOS leads to augmentation of NO production [ xref , xref ]."
31781259
sparser
"Another mechanism could implicate an inactivation of Akt by HNE [ xref , xref ], the phosphorylation of eNOS by Akt on serine-1179 being required for its activation and NO production [ xref ]."
38397830
reach
"To confirm that CORM-2-induced NO production was dependent on eNOS phosphorylation, the pharmacological inhibition of Akt was applied to prevent the phosphorylation of eNOS at Ser 1179 ."
27720892
reach
"Insulin promotes the cardiovascular protective functions of the endothelium including NO production by endothelial NO synthase (eNOS), which it stimulates via Akt kinase which phosphorylates eNOS Ser1179."
19423845
sparser
"The appropriate intracellular localization and distribution of eNOS in PM and Golgi apparatus is necessary for Akt (and agonist)-dependent eNOS phosphorylation on Ser-1179 and is impaired in the cytosolic G2A eNOS (Fulton et al., xref )."
24379783
sparser
"Fulton et al. ( xref ) have demonstrated that the serine/threonine protein kinase Akt (protein kinase B) can directly phosphorylate eNOS on Ser1179 in bovine cells and activate the enzyme."
35137850
sparser
"Moreover, phosphorylation of human eNOS by Akt at Ser 1179 and subsequent release of NO occur at sub-physiological levels of calcium in endothelial cells [28,29] , suggesting that phosphorylation of e[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
24407281
reach
"LY294002, an inhibitor of phosphatidylinositol 3-kinase that initiates Akt catalysed phosphorylation of eNOS on Ser1179, was administered 1 h before the induction of SCI; 24 h after SCI sections were taken for histological examination and for biochemical studies."
21496122
reach
"Phosphorylation of bovine eNOS by AKT and PKB at Ser1179 (Ser1177 in human eNOS) has been shown to upregulate its activity (Fulton et al., 1999; Dimmeler et al., 1999)."
12086862
reach
"30 It has been reported that the serine/threonine protein kinase Akt can directly phosphorylate eNOS on serine 1179 and activate the enzyme, leading to NO production."
28619445
reach
"ENOS is reported to be activated by Akt-dependent phosphorylation of eNOS at Ser 1179 [95] to exert the properties of antiatherosclerosis, including inhibition of smooth muscle cell proliferation, pla[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
25956424
reach
"Akt kinase phosphorylates eNOS at Ser1179, and is likely to be an important in vivo regulator of eNOS activity [33,34] ."
15261488
reach
"Studies showed that Ser 1179 of eNOS is phosphorylated by protein kinase Akt, which increases electron flux through the reductase domain and NO production."
21559738
sparser
"Moreover, administration of endothelial cells with VEGF induced AKT that can phosphorylate eNOS on Ser 1179."
36731150
sparser
"Akt can phosphorylate eNOS on Ser 1179, and that phosphorylation enhances the enzyme’s ability to generate NO ( xref )."
18078008
reach
"A higher Akt activation enhances eNOS phosphorylation at S1177 and/or S1179, thus resulting in an increase in NO-synthesizing activity."
37569855
sparser
"We now report that LPA treatment of bovine aortic endothelial cells (BAEC) activates eNOS enzyme activity in a pathway that involves phosphorylation of eNOS on serine 1179 by protein kinase Akt."
11939794
reach
"Akt can directly phosphorylate eNOS on serine 1179 (based on the bovine eNOS sequence and equivalent to human eNOS-serine 1177) and activate the enzyme, leading to NO production."
21264179
sparser
"In particular, phosphorylation by Akt on Ser1179 of eNOS leads to enhanced activity of the enzyme and, thus, augments production of nitric oxide [31] ."
21906675
rlimsp
"Both vascular endothelial growth factor and FSS activate endothelial protein kinase B (PKB) by way of incompletely understood pathway(s), and, in turn, PKB phosphorylates eNOS at Ser-1179, causing its activation."
11287317
sparser
"30 It has been reported that the serine/threonine protein kinase Akt can directly phosphorylate eNOS on serine 1179 and activate the enzyme, leading to NO production."
28619445