IndraLab

Statements

OTUB1 leads to the deubiquitination of UBE2E1. 9 / 9
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"We show that OTUB1 suppresses UBE2E1 autoubiquitination in vitro and in cells, thereby preventing UBE2E1 from being targeted to the proteasome for degradation."
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"We show here that OTUB1 noncatalytically inhibits UBE2E1 autoubiquitination in vitro in either the presence or absence of E3 ligase (Fig. 4)."
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"OTUB1 non-catalytically stabilizes the E2 ubiquitin-conjugating enzyme UBE2E1 by preventing its autoubiquitination"
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"Although it is not known whether RNF4 stimulates UBE2E1 in vivo, our in vitro results raised the interesting possibility that OTUB1 could inhibit UBE2E1 autoubiquitination in cells, thereby accounting for the ability of OTUB1 to prevent UBE2E1 from being targeted to the proteasome."
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"Catalytically inactive OTUB1 completely inhibits UBE2E1 ubiquitination activity in both the presence and absence of E3 (Fig. 4, top panel)."
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"A recent study showed that OTUB1 also prevents autoubiquitylation of UBE2E1, thereby rescuing the E2 from proteasomal degradation 35."
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"We find that OTUB1 depletion dramatically destabilizes the E2-conjugating enzyme UBE2E1 (UBCH6) in both mouse and human OTUB1 knockout cell lines."
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"OTUB1 inhibits UBE2E1 autoubiquitination."
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"OTUB1 inhibits auto-monoubiquitylation of UBE2E1 and UBE2E2 but not UBE2E3."
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