IndraLab

"The central region of <span class="match term1">UVSSA</span>, which contains the tumor necrosis factor receptor-associated factor (TRAF)-binding motif, is required for the interaction with the N-terminal TRAF domain of <span class="match term0">USP7</span>. Here, we showed that <span class="match term1">UVSSA</span> is mono-ubiquitinated in vitro and identified a lysine residue (Lys414 ) in <span class="match term1">UVSSA</span> as the target of ubiquitination. The deubiquitination activity of <span class="match term0">USP7</span> was inhibited by the ubiquitin-conjugating enzyme UbcH6. Lys414 was also modified by poly-ubiquitin chains in vivo. "

"In contrast to Pol II and UVSSA, CSB is deubiquitinated by USP7 to reverse its ubiquitination state in TC-NER.The physiological significancxe of Pol II degradation may rely on two aspects: for one aspect, the ubiquitination and degradation of RPB1 activate TC-NER and, in parallel, prevents prolonged transcription arrest after sending the TC-NER initiation signal (Nakazawa et al., 2020)."