IndraLab

Statements

CYLD binds MIB2. 21 / 21
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"Here, we report a novel interaction between CYLD and MIB2 and explore the effect of these proteins on Notch signalling."
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No evidence text available
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"Indeed, MIB2 constitutively bound RIPK1 and CYLD before and after TNF stimulation."
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"Characterisation of the CYLD and MIB2 interaction."
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"Here, we report a novel interaction between CYLD and MIB2 and explore the effect of these proteins on Notch signalling."
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No evidence text available
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"The interaction of CYLD-MIB2 was seen both in unstimulated and TNF-alpha stimulated cells, and subsequent experiments were performed without TNF-alpha stimulation ( xref )."
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"CYLD interacts with MIB2."
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"Therefore, the CYLD and MIB2 interaction was investigated in more detail."
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"CYLD interacts with MIB2."
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"The presence of aberrant Notch signalling in skin tumours from patients with BSS further supports the hypothesis that the CYLD and MIB2 interaction might play a pathogenic role in human cancer."
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No evidence text available
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"The potential for a functional interaction between CYLD and MIB2 was investigated further."
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"Given the specificity of CYLD for K63-ubiquitin linked chains [ xref ], our data would suggest that (1) auto-ubiquitylation of MIB2 is most likely a K48-ubiquitin event leading to its proteasomal degradation (in agreement with [ xref ]); (2) the CYLD-MIB2 protein-protein interaction physically prevents efficient auto-ubiquitylation and degradation of MIB2; and (3) MIB2 regulates this process by targeting CYLD for degradation."
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"We assessed the potential role of the CYLD and MIB2 interaction with respect to Notch signalling."
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"The interaction between endogenous CYLD and MIB2 was validated by co-immunoprecipitation of CYLD with MIB2."
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"The potential for a functional interaction between CYLD and MIB2 was investigated further."
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"Here we report a novel interaction between an ubiquitin hydrolase, CYLD, and an ubiquitin ligase, MIB2, and its consequences on Notch signalling."
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"Our data suggests that the CYLD and MIB2 interaction may attenuate Notch signalling by reducing levels of JAG2."
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"To further dissect the nature of the CYLD and MIB2 interaction, we performed ubiquitylation assays, which allowed us to quantify the expression of both proteins and respective ubiquitylated species when catalytically active and inactive CYLD and MIB2 were expressed."
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"The interaction between endogenous CYLD and MIB2 was validated by co-immunoprecipitation of CYLD with MIB2 (Figure xref )."
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