IndraLab

"The enrichment of phosphorylated peptides by TiO2 was performed with tryptic USP14 peptides. The enriched phosphorylated peptides were analyzed on Orbitrap Fusion mass spectrometer (Thermo Scientific). The activation type of HCD was performed for MS2. Protein identification was performed by Thermo Proteome discoverer (v1.4) with Sequest HT. The precursor mass tolerance was set at 10 ppm, and the fragment mass tolerance was set at 0.1 Da. The cysteine carboxyamido methylation was set as a static modification, and phosphorylated serine, threonine, and tyrosine residues were set as variable modifications."