IndraLab

Statements

OTUB1 inhibits UBE2E1. 11 / 11
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eidos
"Although it has been demonstrated that UBE2E1 can stimulate OTUB1 DUB activity ( 28 ) , it has not been shown whether OTUB1 inhibits UBE2E1 in the manner observed for UBE2N / UBC13 , UBE2D2 , and UBE2D3 ( 17 , 18 , 20 ) ."
30282802
eidos
"To test the hypothesis that OTUB1 may directly inhibit UBE2E1 , we assayed the autoubiquitinating activity of UBE2E1 in the presence and absence of OTUB1 in vitro ."
30282802
reach
"These results confirm that OTUB1 can noncatalytically inhibit UBE2E1 as has been observed for UBE2N and UBCH5 isoforms (17–20)."
30282802
sparser
"These results confirm that OTUB1 can noncatalytically inhibit UBE2E1 as has been observed for UBE2N and UBCH5 isoforms ( xref xref xref )."
30282802
eidos
"These results confirm that OTUB1 can noncatalytically inhibit UBE2E1 as has been observed for UBE2N and UBCH5 isoforms ( 17-20 ) ."
30282802
reach
"Although it has been demonstrated that UBE2E1 can stimulate OTUB1 DUB activity (28), it has not been shown whether OTUB1 inhibits UBE2E1 in the manner observed for UBE2N/UBC13, UBE2D2, and UBE2D3 (17, 18, 20)."
30282802
reach
"Recently, UBE2E1 was shown to autoubiquitinate itself in vitro in the presence or absence of the E3 ligase, RNF4 (30, 31), raising the possibility that OTUB1 may stabilize UBE2E1 by preventing autoubiquitination.To test the hypothesis that OTUB1 may directly inhibit UBE2E1, we assayed the autoubiquitinating activity of UBE2E1 in the presence and absence of OTUB1 in vitro."
30282802
eidos
"We show that OTUB1 suppresses UBE2E1 autoubiquitination in vitro and in cells , thereby preventing UBE2E1 from being targeted to the proteasome for degradation ."
30282802
sparser
"Although it has been demonstrated that UBE2E1 can stimulate OTUB1 DUB activity ( xref ), it has not been shown whether OTUB1 inhibits UBE2E1 in the manner observed for UBE2N/UBC13, UBE2D2, and UBE2D3 ( xref , xref , xref )."
30282802
sparser
"To test the hypothesis that OTUB1 may directly inhibit UBE2E1, we assayed the autoubiquitinating activity of UBE2E1 in the presence and absence of OTUB1 in vitro ."
30282802
reach
"We show that OTUB1 suppresses UBE2E1 autoubiquitination in vitro and in cells, thereby preventing UBE2E1 from being targeted to the proteasome for degradation."
30282802