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"Importantly, this work provides proof of concept that potent and selective DUB inhibitors can be achieved and that structural characterization of chemical leads with their cognate DUB target could accelerate probe and drug discovery programs.Detailed methods are provided in the online version of this paper and include the following: Zhang, X.Y., Varthi, M., Sykes, S.M., Phillips, C., Warzecha, C., Zhu, W., Wyce, A., Thorne, A.W., Berger, S.L., and McMahon, S.B."