IndraLab

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FHIT is methylated. 433 / 433
| 433
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"Overall, our study demonstrated that a very high percentage of ATL patient samples (74%) presented with FHIT methylation."
34092254
sparser
"FHIT methylation was also observed in 13% cases in the adjacent control region."
23573237
sparser
"In NSE, there was no promoter methylation of FHIT gene, while in LSCC the rate of it was 24.4% (10/41), and it was related to the tumor pathological grade and lymph node metastasis (P < 0.01)."
16013507
sparser
"DNA methylation of RARB, PGR, RASSF1, CDH1, FHIT, ESR1, C1QTNF6 , PTGS2, SOCS3 , MGMT , and LINE1 was analyzed by pyrosequencing and compared with results from the cell lines RT4, T24, HeLa, BEAS-2B, and HepG2."
23691160
sparser
"The tumor suppressor Fhit protein is defective or absent in many tumor cells due to methylation, mutation or deletion of the FHIT gene."
23993961
sparser
"To further explore the molecular mechanisms between altered TSGs promoter methylation and overexpression of DNMTs protein, we performed a tissue chromatin-immunoprecipitation polymerase chain reaction assay for lung tumors and showed that the methylated FHIT, p16(INK4a) and RARbeta promoters were bound by both DNMT protein and methyl-CpG-binding protein 2."
17140695
sparser
"While no FHIT gene promoter methylation was observed in the matched non-cancerous epithelium (NCE) specimens, 24.6% of DTC samples demonstrated methylation in the FHIT promoter region."
20606729
sparser
"Thus, Fhit is inactivated at an early stage in the development of ESCC ( xref ); FHIT gene promoter methylation has been linked to cigarette smoking ( xref , xref ), and ESCC may be associated with loss of the Fhit protein expression along with alcohol consumption ( xref , xref )."
28693209
sparser
"While methylation of DAP-Kinase and RAR B was equally distributed between tumors with or without schistosomal association, methylation of FHIT, RASSF1 A, E-cadherin, P16, and MGMT tended to be more common in the parasite-associated tumors."
25685453
sparser
"FHIT gene methylation may be one of the molecular events involved in the development and transformation of FMSCs into F6 tumor cells."
26137124
sparser
"Recently a report examining the methylation of CDH13, CDKN2A/p16, FHIT, RARB, RASSF1A and ZMYND10 (BLU) in which methylation of any 2 loci in plasma was considered cancer positive showed 73% sensitivity and 82% specificity [ xref ]."
18947422
sparser
"Our data suggested that FHIT methylation could be a diagnostic biomarker of BC carcinogenesis."
26491255
sparser
"FHIT methylation was significantly associated with p16 methylation in current- and ex-smokers (P = 0.02 and P = 0.01, respectively) with squamous cell carcinoma and in patients with pathologic stage I squamous cell carcinoma (P = 0.001)."
15231689
sparser
"Methylation of CDH1, FHIT, LAMC2, RASSF1A, TIMP3, SFRP1, SOX9, PMF1 and RUNX3 genes is associated with poor survival in muscle-invasive bladder cancer.[ xref xref ] Methylation of RASSF1A and HOXB2 were associated with bladder tumor stage, grade and tumor progression.[ xref xref ] Several studies have examined methylation markers in the urine of patients with bladder cancer."
26604437
sparser
"No relationship was found between FHIT gene methylation and sex, hematologic parameters, chromosomal abnormalities of MDS patients."
18367246
sparser
"FHIT promoter methylation was observed in 37% cases."
23573237
sparser
"Our results indicate that different molecular mechanisms interplay to inactivate FHIT expression and support the proposition that FHIT methylation in normal lung tissue could represent a prognostic marker for progressive disease."
19096006
sparser
"RASSF1A is deleted or methylated in 30–40% of NSCLC and 70–100% of SCLC; FHIT is deleted or methylated in 40–70% of NSCLC and 50–80% of SCLC [ xref ]."
27927793
sparser
"FHIT methylation as a diagnostic tool for the development of ATL."
35663592
sparser
"In addition, aberrant methylation of FHIT can also be induced by nicotine xref , indicating its role in smoking-related ESCC tumorigenesis."
22572016
sparser
"In cellular models of squamous cell carcinoma (SCC), promoter methylation of tumor suppressor p16 and FHIT induces the development of squamous cell carcinoma of the esophagus ( xref - xref )."
22701817
sparser
"The CGI methylation of the FHIT gene was higher at late passage than at early passage in CHA-15, CHA-31, CHA-32, and iPS (FS)-1 cell lines but decreased at the late passage in CHA-20 and H1 cell lines."
34552632
sparser
"In conclusion, FHIT was partially methylated in the HepG2 and Hep3B hepatoma cells."
24396396
sparser
"If physicians could analyze the surrounding tissue for FHIT methylation, it will allow them to know if greater margins need to be excised to prevent future tumor outgrowth."
35663592
sparser
"Therefore, we investigated the association of the status of FHIT promoter methylation and FHIT protein expression with the clinicopathological progression of DTC, using PCR-based methylation assay and immunohistochemical technique."
20606729
sparser
"Strikingly, FHIT methylation was not only found in tumorigenic T-cells, but also in cells and tissue of non-tumor origin."
35663592
sparser
"Further in vivo experiments confirmed that inhibition of DNA methylation of FHIT leads to significant resensitization of the radioresistant oral tumors."
29439529
sparser
"DNA was collected during the asymptomatic carrier stage of these patients and FHIT methylation analyzed and compared to samples from patients with smoldering ATL (Fig.  xref a)."
34092254
sparser
"The levels of p16, RASSF1A and FHIT promoter methylation and the RTL of white blood cells in peripheral blood DNA of 200 healthy individuals and 200 lung cancer patients were analyzed by SYBR Green-based quantitative methylation-specific PCR and quantitative PCR."
27716889
sparser
"The levels of FHIT, RASSF1A and p16 promoter methylation were 3.33 (1.86-6.40) and 2.85 (1.39-5.44) (p = 0.002); 27.62 (9.09-52.86) and 17.17 (3.86-50.87) (p = 0.038); and 0.59 (0.16-4.50) and 0.36 (0.06-4.00) (p = 0.008) in cases and controls, respectively."
27716889
sparser
"According to the authors, because the methylation of the FHIT gene was more common than the presence of HPV infection, which occurred in less than 5% of patients, this gene might play an important role in the pathogenesis of penile squamous cell carcinoma."
23702847
sparser
"Spread to regional lymph nodes correlated significantly with increasing methylation of EFEMP1 (p = 0.027) and FHIT (p = 0.028) with a linear dependence, whereas an inverse correlation was found for CDKN2A methylation (p = 0.05)."
29851970
sparser
"Promoter methylation of p16, TIMP3, RASSF1, RARB, FHIT and APC was a rare event."
18211945
sparser
"Meta-analysis studies of methylation in NSCLC patients and normal patients have shown similar, albeit highly inconsistent findings on FHIT methylation in normal samples [ xref , xref ]."
35663592
sparser
"In conclusion, HPV 16 infection, FHIT methylation and folate deficiency might promote cervical cancer progression."
25422218
sparser
"Finally, 14 articles with 1,345 controls and 945 cases for FHIT expression were involved, while 3 articles with 75 controls and 124 cases for FHIT methylation were included.. xref – xref According to the extracted information of included studies, the frozen liver cancer tissues and normal or adjacent liver tissues were obtained through surgical operation to extract the DNA and detect the expression of FHIT and methylation of FHIT gene."
28790842
sparser
"They studied promoter methylation of p16 and FHIT genes in oral and bronchial brush specimens from smokers enrolled in a randomized placebo-controlled chemoprevention trial."
21292635
sparser
"We identified that the sensitivity value of methylated FAM19A4 and PLR value of methylated RASSF1A , FHIT , MGMT , and p16 are relatively high, suggesting that they are comprehensive parameters for the screening test [ xref ]."
28644424
sparser
"The recent publications on frequencies of FHIT methylation in human cancer have been tabulated [see xref ] as references for those readers particularly interested in studying FHIT."
16928264
sparser
"In hematological malignancies, FHIT methylation has been seen in some cases of myelodysplastic syndrome (MDS), acute myeloid leukemia (AML), and acute lymphoblastic leukemia (ALL), and chronic myelogenous leukemia (CML) [ xref – xref ]."
34092254
sparser
"From logistic regression analysis results, methylation of p16 and FHIT genes was an independent risk factor for hepatocellular carcinoma with odds ratio (OR) values of 10.550 (2.313~48.116) and 8.239 (2.386~28.455), respectively."
30773529
sparser
"Methylation of FHIT gene was frequently found than HPV infection, therefore methylation of the FHIT gene is suggested to play an important role in the pathogenesis of penile squamous cell carcinoma."
18299890
sparser
"Our study identifies germinal FHIT methylation as a signature of ATL disease development that is present in more than 80% of HTLV-I-infected patients with acute or chronic ATL."
34092254
sparser
"Also, other studies have shown that methylated FHIT is correlated with poor prognosis in early ESCC [ xref ]."
30886542
sparser
"In these samples, up to 25% of healthy age-matched whole blood samples from children (5.6 +/−2.9 years) with ALL carried methylated FHIT [ xref ]."
35663592
sparser
"The survival after recurrence in stage 1-2 cancers was also poorer for patients with FHIT methylation than in those without (HR = 2.31; 95% CI = 1.18-7.92; P = 0.03)."
16564166
sparser
"FHIT was methylated in 69% of ESCCs but not in the matched normal tissues, and this methylation was responsible for decreased FHIT protein level xref ."
22572016
sparser
"A combination of AGTR1 and FHIT methylation demonstrated a suitable detection marker of OPSCCs derived from the HPV-infected field, familiar with p16-positive and RB-negative phenotypes."
36344942
sparser
"A longitudinal approach investigating asymptomatic carriers that progress to ATL is the best approach to establish FHIT methylation as a diagnostic marker for ATL disease."
34092254
sparser
"The combination of FHIT and p16INK4a promoter methylation has also been associated with recurrence in Stage I NSCLC [ xref ]."
24686037
sparser
"Recently, Mielcarek-Kuchta et al. demonstrated that, by using methylation specific PCR (MSP), promoter methylation of FHIT was only observed in 1.8% of 53 cases of oral and oropharyngeal cancer [ xref ]."
25460508
sparser
"Given that FHIT methylation was only seen in ATL patient samples, and not asymptomatic or TSP/HAM patient samples, again confirms its specific role in tumor initiation and/or maintenance."
34092254
sparser
"The result about Caucasian population from datasets is consistent with the result from meta-analysis, so the relationship between FHIT methylation and NSCLC in Caucasian population is robust."
28036263
sparser
"It is interesting that the frequency of FHIT methylation (54.5%) shown here correlates with the previously reported frequency of complete loss of FHIT expression (51%) in clear cell renal carcinom[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18378390
sparser
"The status of FHIT methylation was not associated with clinical stage and differentiation grade, there was no significant difference between the malignant and borderline tumors."
16181544
sparser
"FHIT methylation was found in 49 (51.58%) cases, and 40 of 49 (81.63%) were demonstrated deletion of the FHIT protein."
18365557
sparser
"FHIT was methylated in the early stages of ESCC, and its methylation was associated with poor prognosis [ xref ]."
27110300
sparser
"We performed a comprehensive analysis on FHIT gene methylation and expression in uncultured peripheral blood mononuclear cells (PBMC) isolated from healthy volunteer donors (HD), HTLV-I infected asymptomatic carriers (AC), and HTLV-I infected individuals diagnosed with tropical spastic paraparesis/HTLV- I-associated myelopathy patients (TSP/HAM) and adult T-cell leukemia (ATL)."
34092254
sparser
"We studied FHIT methylation in 254 NSCLCs in order to further understand the clinicopathological and prognostic significance of FHIT methylation in NSCLC."
15231689
sparser
"FHIT methylation in normal lung was associated with an increased risk of progressive disease (OR, 2.27; P = 0.0415)."
19096006
sparser
"While we did not measure methylation of ERa; we did find that active smoking at different points in the life course was associated with increased odds of methylation of GSTP1, FHIT, and CDKN2A ."
30617699
sparser
"To test the diagnostic ability of FHIT methylation status on NSCLC, thirteen studies, including 2,119 samples were included in our meta-analysis."
28036263
sparser
"While further longitudinal studies are required, it does open the possibility that FHIT methylation could also predict ATL development not only in ACs, but in other HTLV-I associated diseases."
34092254
sparser
"Our results suggest that methylated FHIT may play a major role in the pathogenesis of ATL and for the first time, clearly represents an epigenetic signature that differentiates TSP/HAM (7.35%) from acute and chronic ATL diseases."
34092254
sparser
"The methylation analysis of FHIT was carried out in a large sample size of patients with CD, and we analyzed the clinical relevance of FHIT methylation in relation to CD clinical parameters (disease duration and location at diagnosis)."
32375395
sparser
"Our data provide information on the biological and clinical implications of DNA hypermethylated genes in CD, identifying FHIT methylation as a promising new biomarker for CD."
32375395
sparser
"Both MBDcap-PCR (Fig. xref ) and bisulphite pyrosequcening (Fig. xref ) confirmed higher FHIT promoter methylation in OML1-R than in OML1-P cells."
25460508
sparser
"Recently, detection of FHIT methylation identified by meta-analyses was shown to be useful for the early diagnosis of breast carcinoma [ xref ] and NSCLC [ xref ]."
32375395
sparser
"We wanted to determine whether FHIT methylation occurs in ATL cells only because of ATL disease progression or if FHIT methylation is a preexisting germinal trait that may predispose some infected individuals to develop ATL diseases."
34092254
sparser
"A total of 17 papers that evaluated the associations of FHIT expression (14 articles) and FHIT gene methylation (3 articles) with liver cancer pathogenesis were included in this meta-analysis."
28790842
sparser
"However, early indicators suggest that in those HTLV-I carriers that possess FHIT methylation, these patients will develop ATL disease in the next 20-40 years."
35663592
sparser
"Two sets of primers were used as reported earlier [32] , one was specific for DNA methylated at the promoter region of FHIT gene and the other specific for unmethylated DNA."
21095196
sparser
"These results suggested that promoter methylation of FHIT is partially controlled by EZH2 in the promoter region of FHIT ."
25460508
sparser
"Consistent with these results, we found approximately 20% of ATL lymphoma patients had methylated FHIT."
34092254
sparser
"In this study, DNA methylation of FHIT, serum folate level and HPV16 status were measured using methylation-specific polymerase chain reaction (MSP), radioimmunoassay (RIA) and polymerase chain reaction (PCR), respectively, in 310 women with a diagnosis of normal cervix (NC, n=109), cervical intraepithelial neoplasia (CIN, n=101) and squamous cell carcinoma of the cervix (SCC, n=101)."
25422218
sparser
"Twenty out of the 22 cases negative for FHIT protein expression showed FHIT methylation."
23702847
sparser
"An interesting finding from our study was that a small percentage (over 5%) of PBMCs from normal, otherwise healthy donors, presented with methylated FHIT."
35663592
sparser
"MSP confirmed methylation of FHIT detected by MS-MLPA for UT-SCV-2 and UT-SCV-4."
19047905
sparser
"Furthermore, the extent of FHIT methylation in ATL patients was quantitatively comparable in virus-infected and virus non-infected cells."
34092254
sparser
"Methylated FHIT gene was observed in 37% (23/62) cases of CRC."
23573237
sparser
"FHIT methylation may play a role in lung cancer later metastatic stages while GSTP1 methylation may rather play a role in the early pathogenesis."
24935385
sparser
"The frequency of DNA methylation reported for FHIT using MSP was 39% (Range 8%-80%) [ xref , xref – xref ]; however similar to our findings, Wisman et al . reported an absence of FHIT methylation in SCC using QMSP[ xref ]."
25826459
sparser
"Yanagawa et al. also compared the relationship between methylation of 10 cancer-related genes and mutations of EGFR, KRAS, and TP53 in 62 lung adenocarcinoma cases from smokers and never-smokers and f[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
30089603
sparser
"The results of the current study suggest that methylation of FHIT is a useful biomarker of biologically aggressive disease in patients with NSCLC."
15042681
sparser
"No association between FHIT gene methylation and clinical variables such as age, gender and clinical stage was found."
16313263
sparser
"Inactivation of FHIT by promoter hypermethylation plays an important role in tumorigenesis in several types of tumors including NSCLC. xref , xref – xref To date, there have been some studies describing the methylation status of FHIT in NSCLC; however, the roles of methylation of FHIT in NSCLC and clinical significance have not been thoroughly investigated."
26929601
sparser
"Besides, methylation of FHIT is another important mechanism for loss of Fhit expression ( xref )."
15150628
sparser
"Sub-classification of ATL disease into acute, chronic, and smoldering subtypes demonstrated strong FHIT gene methylation in acute and chronic ATL patient samples, and moderate FHIT gene methylation in smoldering ATL patient samples (Fig. xref b)."
34092254
sparser
"A consideration for future studies is that the level of FHIT methylation in the public is likely to be low."
35663592
sparser
"Overall, this suggests that FHIT methylation is likely present in patients, prior to HTLV-I infection, and predisposes HTLV-I carriers to ATL development."
35663592
sparser
"Promoter aberrant methylation of FHIT is an important mechanism for inactivation of this tumor suppressor gene in mammary tumorigenesis."
21095196
sparser
"Yet another group reported differential methylation of CDH1 , DAPK1 , CDKN2A , MGMT , ICDKN2B , FHIT , APC , RASSF1 , GSTP1 , RARB , and TP73 in bladder cancer specimens, with schistosomiasis-associated specimens featuring more differentially methylated genes than those not associated with this infection [ xref ]."
26042665
sparser
"Of the remaining 28 articles, 3 did not have the frequency of FHIT expression in case group and control group, 2 contained duplicate data, the samples of 4 were inappropriate, and 2 publications were about FHIT gene methylation and did not have sufficient frequency of FHIT gene methylation."
28790842
sparser
"In addition, LOH of Rb has been found only in the anaplastic component. xref xref Aberrant promoter methylation in the fragile histidine triad (FHIT) gene is seen only in highgrade dedifferentiated components."
24932031
sparser
"To confirm that our MSPCR results represented the methylation density of these genes, we also performed BSG and found strong FHIT gene methylation in ATL patients compared to CDKN1A, CDKN2A, and SHP1 (Fig. xref c)."
34092254
sparser
"The FHIT methylation is associated with the p16 methylation and smoking in squamous cell carcinoma, suggesting that FHIT may cooperate with p16 for the development of squamous cell carcinoma of lung in individuals exposed to tobacco smoke."
15231689
sparser
"The PCR product with specific primers for methylated FHIT was detected only with DNA from tumour samples but not with DNA from normal renal tissues ( Fig. 1 )."
18378390
sparser
"It is also possible, though doubtful, that virus related factors produced by HTLV-I-infected cells are responsible for FHIT methylation."
34092254
sparser
"Before treatment of 5-Aza-dC, FHIT gene methylation was detected in HepG2 cells, while no FHIT mRNA and protein expression was detected."
19728916
sparser
"Taken together, our data on the frequent hypermethylation of the FHIT 5′CpG island and the significant decrease of FHIT expression in samples with a methylated FHIT 5′CpG island indicate that hy[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18378390
sparser
"Though FHIT methylation was significantly higher in primary NSCLC samples, the level of FHIT methylation in control samples varied widely."
35663592
sparser
"All samples were found to harbor FHIT methylation."
35663592
sparser
"Our results indicate that: (1) aberrant, widespread methylation of CpG islands increases with the histological progression of serrated adenomas; (2) methylation of SLC5A8 is an early event; and (3) additional methylation of the p16, p14, MGMT, TIMP3, and FHIT genes are important tumorigenic steps in the serrated neoplasia pathway."
15389252
sparser
"Methylation of the FHIT, RASSF1A and RAR-β genes in DNA from all samples and the corresponding gene methylation status were assessed using the methylation-specific polymerase chain reaction (PCR, MSP)."
25040980
sparser
"In this study, we confirmed methylation of the FHIT gene in human esophageal squamous epithelial cells (HEECs) and examined whether nicotine induced alteration of FHIT."
16570269
sparser
"The MDS patients with FHIT gene methylation had significantly shorter survival time than those without FHIT methylation (20.0 months vs. 40.0 months, P=0.025)."
18367246
sparser
"FHIT methylation in 125 squamous cell carcinomas (of 254 studied cases) was associated with exposure to tobacco smoke and p16 methylation [ xref ]."
18363846
sparser
"Interaction analysis showed that the ORs only with FHIT methylation (OR=11.47) or only with HPV 16 positive (OR=4.63) or with serum folate level lower than 3.19ng/ml (OR=1.68) in SCC group were all higher than the control status of HPV 16 negative and FHIT unmethylation and serum folate level more than 3.19ng/ml (OR=1)."
25422218
sparser
"In this study, high promoter methylation of FHIT (>10%) can be observed in 55% (22/40) of oral cancer patient samples."
25460508
sparser
"In contrast, only 7.35% for TSP, 5.6% for asymptomatic carriers, and 5.1% of healthy donor samples displayed FHIT methylation (Fig. xref c)."
34092254
sparser
"The FHIT gene methylation was observed in 21 of the 48 patients (44%)."
16313263
sparser
"The purpose of this study was to investigate aberrant promoter methylation of the p16 and FHIT genes in tissues and plasma and loss of protein expression in esophageal precancerous conditions (EPC) and esophageal squamous cell carcinoma (ESCC) of high-risk area."
18365557
sparser
"Only 15% (9/62) patients did not show the presence of Kras mutation or RASSF1A, FHIT and MGMT methylation."
23573237
sparser
"The promoter methylation of DAPK1, FHIT, MGMT, and CDKN2A was investigated by using a methylation-specific polymerase chain reaction in 53 specimens of cervical cancer (42 squamous cell carcinoma, 11 adenocarcinoma), 22 specimens of intraepithelial neoplasia tissues, and 24 control normal cervical tissue specimens."
23494221
sparser
"Longitudinal studies following FHIT methylation are the next step in validating these results."
35663592
sparser
"Also, the frequency of promoter methylation of p16INK4a , MGMT , RASSF1A , MTHFR , and FHIT is higher in the NSCLC tumors of smokers relative to non-smokers [ xref , xref , xref – xref ]."
24686037
sparser
"Whether FHIT methylation is the ultimate factor predisposing these individuals to carcinomas is unknown."
35663592
sparser
"Nicotine administration in human bronchial epithelial cells significantly upregulates several genes particularly associated with activation of mitogen activated protein kinase (MAPK).[ xref ] Nicotine increases methylation of the fragile histidine triad (FHIT) gene resulting in decreased FHIT and increased deoxyribonucleic acid (DNA) methyltransferase (DNMT) 3a expression.[ xref ] Notably, removal of nicotine restores normal methylation patterns and expression suggesting the effect of nicotine is reversible."
23599683
sparser
"Since the percentage of circulating ATL cells is very low in the lymphoma type (less than 5%), these results strongly suggest that non-infected PBMC from lymphoma-type ATL carry a methylated FHIT gene."
34092254
sparser
"The importance of FHIT methylation in solid cancers has been documented."
35663592
sparser
"Both tissue and plasma groups showed significant association between FHIT methylation and NSCLC (OR = 3.68 and 3.89, respectively) (Figure xref ), which suggested that FHIT methylation test is a promising biomarker for NSCLC diagnosis with either tissue or plasma samples."
28036263
sparser
"In the literature, methylation of FHIT, GSTP1, and MGMT frequently occurs in NSCLC ( xref , xref , xref , xref , xref ) but in the current study, methylation of these genes was rarely detected."
19945765
sparser
"Importantly, patients with a negative staining of FHIT demonstrated a significantly higher promoter methylation of FHIT than that of patients with positive staining of FHIT ( P = 0.0395, Fig. xref )."
25460508
sparser
"The present study demonstrated 46.4% FHIT methylation in the ALL cases."
24137446
sparser
"The fact that FHIT is methylated in ATL patients across all geographical regions demonstrates its usefulness as a broad prognostic marker for the development of ATL disease."
34092254
sparser
"This study explored the relationship between promoter methylation and inactivation of the DAPK1, FHIT, MGMT, and CDKN2A genes in cervical cancer."
23494221
sparser
"Along with our data, there is existing evidence that FHIT methylation occurs in normal adult cells and tissue."
35663592
sparser
"Strikingly, we also discovered that nearly 6% of HTLV-I carriers had a methylated FHIT gene."
35663592
sparser
"Additionally, RASSF1A is deleted or methylated in 30–40% of NSCLC and 70–100% of SCLC [ xref ], FHIT is deleted or methylated in 40–70% of NSCLC and 50–80% of SCLC [ xref ], and TSLC1 is methylated in an estimated 85% of NSCLC [ xref ]."
24686037
sparser
"The patients with FHIT methylation and folate deficiency or with FHIT methylation and HPV 16 positive were SCC or CIN, and the patients with HPV 16 positive and FHIT methylation and folate deficiency were all SCC."
25422218
sparser
"The pooled odds ratio of FHIT promoter methylation in cancer samples was 3.43 (95% CI: 1.85 to 6.36) compared with that in controls."
28036263
sparser
"Active smoking between age 41–50 was associated with increased odds of methylation of FHIT (OR= 4.64, 95%CI: 1.60; 13.51)."
30617699
sparser
"Univariate analysis revealed adverse prognostic factors: FHIT gene methylation (P = 0.028), poor performance status (I to IV, P = 0.002), anemia (< or =8.5 g/dL, P = 0.007), hypoalbuminemia (< or =3.5 g/dL, P < 0.002), high serum C-reactive protein levels (>0.5 mg/dL, P = 0.002), elevated beta-2-microglobulin serum levels (>6.5 mg/L, P < 0.001), and treatments not including autologous peripheral blood stem cell transplantation (auto-PBSCT) (P = 0.007)."
16313263
sparser
"The abnormal methylation of the FHIT gene was found in 26 of 55 (47.2%) MDS cases, but it was not in normal control."
18367246
sparser
"RARβ and FHIT promoter methylation may be associated with the carcinogenesis of cervical cancer."
28639889
sparser
"Methylation of the FHIT gene corresponded to loss of FHIT expression in ATL patient samples."
34092254
sparser
"A relatively high frequency of CpG promoter methylation (52%) was found only in hTERT, with low frequency of methylation (<15%) in p16INK4a , TIMP3 , RASFF1 , and RARB , and no methylation in FHIT and APC .[ xref ] Given the presence of promoter methylation of APC , RASSF1 , and RARB in cutaneous melanoma, these findings suggest that cutaneous and UM likely undergo different epigenetic changes."
29578129
sparser
"In addition, the effects of FHIT and p16 gene methylation on the diagnostic accuracy of liver cancer were calculated."
30773529
sparser
"Methylation of FHIT, RASSF1A and RARβ genes may be related to progression of lung oncogenesis."
25040980
sparser
"FHIT methylation was only found in all three stages of the tumors but was significantly more pronounced in IC (P = 0.041 vs IB)."
15574200
sparser
"In family A, the father died from ATL disease; however, we were able to obtain samples from a sibling, a wife, and three children that were all asymptomatic carriers for HTLV-I. Examination of FHIT methylation in all asymptomatic carriers of the family showed strong FHIT methylation in the sibling and son, with lower methylation in the 2nd son and daughter."
34092254
sparser
"However, in a study of FHIT methylation in cervical cancer, the methylation pattern identified by bisulfite genomic sequencing did not correlate with the MSP [ xref ]."
16928264
sparser
"Differential patterns of WWOX and FHIT methylation were observed in neoplastic vs adjacent non-neoplastic tissues, suggesting that targeted MSP amplification could be useful in following treatment or prevention protocols."
15674328
sparser
"We investigated the aberrant promoter methylation of FHIT in lung cancer in the Han population of southern-central China."
20814237
sparser
"However, significant odds ratios between FHIT promoter methylation and NSCLC were still retained in most of the subgroups, which is in accordance with the overall meta-analysis results ( xref )."
28036263
sparser
"Given the role of FHIT in early cancer initiation, it will be exciting to see if diagnostic tools for FHIT methylation could play a larger role in determining initial cancer development."
35663592
sparser
"Significant reduction of FHIT expression was detected for all tested tumours with methylated FHIT 5′CpG island compared to tumours with unmethylated FHIT 5′CpG island and non-malignant tissues."
18378390
sparser
"FHIT promoter methylation may play a crucial role in cervical cancer progression."
28639889
sparser
"The aim of the present study was to evaluate the influences of folate on FHIT gene methylation and expression in the progression of cervical cancerization."
29434897
sparser
"Cell proliferation and apoptosis were determined by viable cell counting and flow cytometry while FHIT gene DNA methylation was used with methylation specific PCR (MSP)."
25059370
sparser
"We report and discuss the results of p16, DAPK, MGMT, FHIT and APC methylation analysis in the 126 first patients: 77 at high risk of cancer and 49 followed up after primary cancer resection."
16043258
sparser
"It has already been suggested that some environmental factors, such as smoking can influence FHIT methylation [ xref ]."
35663592
sparser
"The methylation-specific PCR results demonstrated that the promoter methylation of FHIT genes existed in the F6 cell line."
26137124
sparser
"It may be inferred that WWOX methylation does not affect the methylation of FHIT and p73."
24137446
sparser
"With increasing degrees of cervical lesions, the HPV16 infection rate (χ(2)=47.623, P<0.001), FHIT methylation rate (χ(2)=40.147, P<0.001) and the rate of FHIT protein low expression (χ(2)=65.098, P<0.001) were all gradually increasing."
27346116
sparser
"These results suggested that aberrant methylation of the FHIT gene might be one of molecular events involved in the disease progression of MDS and be an adverse prognostic factor in MDS."
18367246
sparser
"Tumor stage, metastasis and presence of lymphatic invasion were significantly linked with the presence of methylated FHIT (p-values 0.001, 0.047 and 0.004, respectively)."
23573237
sparser
"Therefore, we advocate that methylated SOX17 , CDO1 , ZFP42 , TAC1 , FAM19A4 , FHIT , MGMT , p16 , and RASSF1A are useful in the screening and auxiliary detection of lung cancer."
28644424
sparser
"Why some patients escape FHIT methylation is currently unknown."
34092254
sparser
"For this purpose, we studied the expression of the FHIT gene by semi-quantitative RT-PCR in three tumour samples with methylated FHIT 5′CpG island, three tumour samples with unmethylated FHIT 5′[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18378390
sparser
"In addition, the much more significant difference of FHIT promoter methylation between NSCLC and normal controls was observed in our meta-analysis and in Wu's as well as in Yan's study [ xref ]."
28036263
sparser
"FHIT methylation and protein expression levels were detected by methylation specific PCR (MSP) and Western Blot, respectively."
27346116
sparser
"Frequent methylation of the both FHIT and p15 genes has been reported by other investigators ( xref , xref , xref , xref , xref )."
21592569
sparser
"We investigated whether FHIT methylation is associated with aberrant expression of Fhit protein and acetylated histone, and whether aberrant expression of Fhit protein and acetylated histone are related to prognosis after resection for esophageal squamous cell cancer."
16863736
sparser
"However, when normal breast tissue was analyzed, no FHIT methylation was seen."
35663592
sparser
"Our own result indicates that methylated alleles were found in all tumor and normal DNA (Figure xref ) i.e. FHIT methylation was not tumor-specific."
16928264
sparser
"Interestingly, patients with CD and FHIT methylation showed poor outcomes among patients with CD who had been diagnosed over a 5-year period ( n = 88), but the difference was not statistically significant ( xref )."
32375395
sparser
"The present meta-analysis demonstrates that methylated SOX17 , CDO1 , ZFP42 , TAC1 , FAM19A4 , FHIT , MGMT , p16 , and RASSF1A are potential superior biomarkers for the screening and auxiliary detection of lung cancer."
28644424
sparser
"FHIT methylation was associated with high WBC counts at diagnosis, a known prognostic indicator."
15026336
sparser
"These findings suggest FHIT methylation is associated with a higher susceptibility and has a prognostic significance in early stage lung cancer in the Han population of southern-central China and may represent a marker for progressive disease."
20814237
sparser
"However, when we randomly selected ATL patients for sequence analysis, we found that FHIT gene methylation displayed three peaks with very strong CpG island methylation that corresponded to regions within and just outside exon1 (Fig. xref f)."
34092254
sparser
"In our study, only 5% of PBMCs had methylated FHIT."
35663592
sparser
"In all, 17 eligible articles on RARβ and FHIT promoter methylation were identified in the study."
28639889
sparser
"The promoter methylation of FHIT was lower in patients with LUAD and LUSC."
35254116
sparser
"Methylation of the ATM, HIC1, CDKN1B, PTEN and FHIT genes was not detected in any tumor sample, whereas low frequencies were observed for the DAPK1 (3 samples), CHFR, IGSF4, GSTP1 (2 samples), CDKN2A, RARβ, CASP8, VHL and TP73 (1 sample) genes."
23226780
sparser
"Methylation of the FHIT gene is associated with transcriptional inactivation in esophageal squamous cell carcinoma, and FHIT inactivation has been linked to smoking-related carcinogenesis."
16570269
sparser
"Importantly, EZH2-depleted cells also showed a 10%-decrease of FHIT promoter methylation as demonstrated by bisulphite pyrosequencing (Fig xref )."
25460508
sparser
"Proliferation and apoptosis of cells, methylation of FHIT gene and the levels of FHIT protein expression were measured in each group."
25975556
sparser
"Moreover, RARβ , p16INK4a , FHIT , and RASSF1A promoter methylation increases with increasing smoking intensity [ xref , xref – xref ]."
24686037
sparser
"In 5/10 ccRCCs clinical tumors, FHIT was found to be hypermethylated in ccRCC, however due to the limited number of samples the authors were not able to establish a correlation of FHIT methylation to any clinical parameters [ xref ]."
23867515
sparser
"The correlation of FHIT methylation and clinicopathological features was analyzed."
16784654
sparser
"Interestingly, difference was found between Asian (3.50, 95% CI: 1.50 - 8.14, P = 0.005) and Caucasian population (2.55, 95% CI: 0.86 - 7.57, P = 0.09) subgroup (Figure xref ), and the differential methylation in Caucasian population is not significant, indicating that diagnostic ability of FHIT methylation might be limited in Caucasian population."
28036263
sparser
"The concordances between methylation of Cyclin D2 and the methylation of RARbeta, GSTP1, CDH13, RASSF1A, and APC were statistically significant, whereas methylation of P16, DAPK, FHIT, and CDH1 were not significant."
14581343
sparser
"To confirm that methylation-specific PCR accurately reflected the methylation status of the FHIT 5′CpG island, we performed bisulfite sequencing of the FHIT 5′CpG island for 3 tumour samples that [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18378390
sparser
"Next, we examined CD25−/+ fractions for FHIT gene methylation by BSG."
34092254
sparser
"The adjusted pooled OR after the trim and fill analysis was 2.09 (95% CI: 1.10 - 3.96, P = 0.024) in the random effects model indicating a significantly positive association between FHIT methylation and NSCLC ( xref )."
28036263
sparser
"Conversely, the level of FHIT expression was significantly decreased in all tumours with methylated FHIT 5′CpG island."
18378390
sparser
"Active smoking at certain ages was associated with increased methylation of GSTP1, FHIT, and CDKN2A and decreased methylation of SCGB3A1 and BRCA1 among both pre- and postmenopausal women."
30617699
sparser
"Aberrant methylation of FHIT was found in 85 (33%) of 257 esophageal cancer patients."
16564166
sparser
"Subgroup analysis showed that FHIT methylation is significantly relevant to NSCLC in Asians (OR = 3.50, 95% CI: 1.50 - 8.14) but not in Caucasian population (OR = 2.55, 95% CI: 0.86 - 7.57), indicating that aberrant methylation of FHIT can be a diagnostic biomarker for NSCLC in Asian population."
28036263
sparser
"In contrast, FHIT methylation in infant and non-infant ALL patients carrying germ line MLL was found in only approximately 60% (P< or =0.004)."
16357833
sparser
"It is also interesting to note that in our study less than 3% of healthy donors carry a methylated FHIT gene."
34092254
sparser
"The frequencies of gene methylation in genes p16, APC1A, FHIT, hMLH1 and CDH1 were 29.2%, 34%, 60.4%, 10.9% and 79.8%, respectively."
19268989
sparser
"The random effects models were applied for methylated RASSF1A , APC , RARβ , CDH13 , and FHIT (I 2 > 50%, P < 0.1)."
28404957
sparser
"Methylation of the FHIT gene has been observed in several solid tumors and an abridged or complete loss of expression of FHIT protein has been shown to be due to gene methylation leading to its transcriptional inactivation and disease progression xref ."
23573237
sparser
"FHIT methylation was predominantly detected in tumors with vascular invasion (21 of 44, 48%, P = 0.0703) or lymphatic permeation (28 of 59, 47%, P = 0.0115)."
15541815
sparser
"Majority of the cases with methylated FHIT gene were tumor stage 3 (70%) and moderately differentiated histological grade (65%)."
23573237
sparser
"These included the study of Haroun et al. ( xref ) that identified FHIT methylation and that of Hetta et al. ( xref ) which reported circulating microRNA-17 and microRNA-22 as potential biomarkers for early detection of lung cancer."
33659210
sparser
"AGTR1 and FHIT were frequently methylated in HPV-positive OPSCC samples with a good area under the curve (AUC over 0.70)."
36344942
sparser
"The SVM and DT models for diagnosing lung cancer were successfully developed through the combined detection of p16, RASSF1A and FHIT promoter methylation and RTL, which provided useful tools for screening lung cancer."
27716889
sparser
"Therefore, in order to get more precise conclusions, we aimed to conduct a systematic meta-analysis to clarify the role of FHIT expression and FHIT gene methylation in the pathogenesis of liver cancer."
28790842
sparser
"These data clearly call for the development of ATL screening tests based off FHIT methylation."
35663592
sparser
"Methylation-specific PCR (MSP) was used to detect the methylation of FHIT and p16 genes in the 2 groups."
30773529
sparser
"Moreover, HTLV-I infected carriers, and those that presented with TSP/HAM, a neurological disease, rarely carried methylated FHIT."
35663592
sparser
"FHIT is frequently methylated in the early stages of ESCC, and aberrant methylation of FHIT is associated with poor prognosis and tobacco exposure [ xref ]."
27110300
sparser
"While studying the role of epigenetic factors in ATL, we discovered that the fragile histidine triad gene, FHIT, was highly and statistically methylated in acute (78%) and chronic (89%) ATL disease [ xref ]."
35663592
sparser
"Aberrant methylation of p16INK4a, FHIT, APC, MLH1, RASSF1, CDKN2A, and DAPK has been associated with lung cancer stage, metastasis, and an increased risk of recurrence after therapy [ xref ]."
18834524
sparser
"Indeed, several studies have revealed that methylated genes--including CDH1, FHIT, LAMC2, RASSF1A, TIMP3, SFRP1, SOX9, PMF1 and RUNX3--are associated with poor survival in patients with MIBC."
23628807
sparser
"If this is the case, FHIT methylation may be found in family members, exposing children of ATL affected family members to development of ATL."
34092254
sparser
"Thus, the methylation of FHIT and CDH13 had a relationship with liver cancer incidence."
32474857
sparser
"FHIT gene methylation strongly correlated with lower FHIT mRNA expression compared to TSP patient and HD samples (Fig. xref h); and ATL patient samples with an unmethylated FHIT gene expressed lower levels compared to methylated ATL patient samples (Fig. xref i)."
34092254
sparser
"Promoter methylation of APC and FHIT has been associated with reduced survival in ESCC patients after esophagectomy."
27286455
sparser
"Therefore, we innovatively integrated the high-throughput data and published articles to assess and validate the diagnostic ability of FHIT methylation test in NSCLC."
28036263
sparser
"Recent evidence indicates that inactivation of FHIT by promoter hypermethylation plays an important role in tumorigenesis in several types of tumors including BC. xref – xref However, the reported correlation between FHIT methylation and clinicopathological features in BC was inconsistent."
26491255
sparser
"In a study of OSCCs associated with betel and/or tobacco use ( xref ), 28% of samples exhibited FHIT promoter methylation, and 36% of cancers and 50% of premalignant lesions showed aberrant RT–PCR products, suggesting that FHIT aberration could be an early event in oral carcinogenesis."
12771912
sparser
"None of the other nine candidate genes were methylated in seminomas, but MGMT (44%), APC (29%) and FHIT (29%) were frequently methylated in NSTGCTs."
12545168
sparser
"Also, a low frequency of methylation of the APC , RARB , and FHIT genes was detected in NSGCTs."
12495446
sparser
"FHIT methylation has been identified in various types of cancer, including colon cancer; to the best of our knowledge, this is the first report implicating aberrant DNA hypermethylation of FHIT in IBD pathogenesis."
32375395
sparser
"Furthermore, the WWOX, FHIT and p73 methylation frequency was examined in the ALL patients classified by their diagnosis status of NDP, CRP and RP."
24137446
sparser
"In the present study, 14 studies were included to investigate the correlation between FHIT expression and liver cancer risk and 3 studies were for FHIT gene methylation."
28790842
sparser
"FHIT methylation in squamous cell carcinoma occurred at a 4.62 times (95% CI = 1.26 - 34.97; P = 0.02) higher prevalence in current smokers than in never-smokers."
15231689
sparser
"Methylation-specific polymerase chain reaction, messenger RNA (mRNA) expression analysis, immunohistochemistry, and Western blot analysis were performed to study the methylation of p16 and FHIT genes in 351 pairs of malignant/normal breast tissues."
19956388
sparser
"They observed significantly decreased expression of FHIT in tumor samples with methylated FHIT gene using semi-quantitative RT-PCR whereas the unmethylated samples showed FHIT expression at high levels."
26198328
sparser
"Overall, these results clearly demonstrate that the FHIT gene is methylated in the majority of ATL patient samples, which correlates with decreased FHIT expression in ATL patient samples."
34092254
sparser
"Methylation of FHIT was not detected in the cfDNA of the control group, while methylation of the APC and RASSF1 genes was found in 1 out of 15 (6.7%) healthy donors."
27725787
sparser
"No significant association involving 290 patients with NSCLC and 186 controls was found between FHIT methylation and NSCLC ( P = 0.073) (Table xref )."
28404957
sparser
"The FHIT methylation was observed in 33(55%) specimens, and the aberrant expression of Fhit and acetylated H4 was found in 42 (70%) and 40 (67%) specimens, respectively."
16863736
sparser
"However, we failed to observe any association between tumor grade and methylated FHIT ( xref )."
23573237
sparser
"FHIT methylation is associated with poor prognosis in ESCC."
27110300
sparser
"Moreover, patients with a higher FHIT methylation (>10%, n=22/40) showed a lower locoregional control rate than that of patients with lower methylation ( P = 0.038; Fig. xref )."
25460508
sparser
"Previous data related to cervical cancer showed that DAPK1, FHIT, MGMT, CDKN2A, CADM1 and MAL were frequently methylated genes in cervical carcinogenesis ( xref , xref )."
25845528
sparser
"Patients with methylated FHIT gene had a higher incidence of non-vegetarian and fatty diet as compared to the unmethylated group (61% and 52% vs. 41% and 51%, respectively)."
23573237
sparser
"Due to the previous limitations, we strongly recommend to use more advanced methylation detection methods, like WGBS (whole genome bisulfite sequencing) and RRBS (restricted region bisulfite sequencing), to explore the association between FHIT promoter methylation and NSCLC with larger sample sizes."
28036263
sparser
"In fact, silencing of the MMACHC ( xref ) and CPEB1 ( xref ) genes due to DNA methylation, and gene deletion and DNA methylation of FHIT ( xref ), have been reported in human cancers."
25230976
sparser
"In this study, we performed a meta-analysis to evaluate the ability to use FHIT methylation level for early lung cancer diagnosis."
28036263
sparser
"The present study was undertaken to determine whether methylation and expression of p16 and FHIT genes would correlate with the estrogen receptor (ER) and progesterone receptor (PR) status."
19956388
sparser
"As expected, a significant association was found between FHIT methylation and NSCLC in meta-analysis (OR = 3.43), indicating the existence of a strong association between FHIT promoter methylation and lung cancer."
28036263
sparser
"Moreover, methylation of MTHFR , CBS , MGMT , P16 , FHIT , and RASSF1A is closely related to esophageal cancer tumorigenesis."
30123355
sparser
"FHIT methylation was significantly correlated with loss of FHIT mRNA expression by Northern blot analysis in lung cancer cell lines and with loss of Fhit expression in NSCLC and breast tumors by immunostaining."
11325823
sparser
"No prognostic effect of FHIT methylation was observed in stage I and stage II NSCLCs."
15231689
sparser
"We analyzed ATL samples with unmethylated or weak methylated FHIT and found only 1 patient with a deletion of exon 5, and none with deletions in exon 8, demonstrating that the majority of unmethylated ATL patients’ sample have an intact FHIT gene, with no loss of FHIT integrity in these regions (Suppl xref )."
34092254
sparser
"There was also a relationship between smoking and drinking and the methylation of FHIT and CDH13 (P<0.05)."
32474857
sparser
"In 100% of the infant MLL cases tested, methylation of the FHIT 5'CpG region was observed, resulting in strongly reduced mRNA and protein expression."
16357833
sparser
"Furthermore, BLU methylation was associated with an increased level of alpha-fetoprotein, and FHIT methylation was inversely correlated with HCC recurrence."
22766745
sparser
"However, our data suggests that they are at a higher risk of developing ATL in their lifetime due to the strong correlation between ATL disease and FHIT methylation presented in Fig. xref ."
34092254
sparser
"FHIT methylation was detected in 37% of primary NSCLCs, 31% of primary breast cancers, and 65% of lung and 86% of breast cancer cell lines."
11325823
sparser
"Next, we investigated if FHIT methylation was associated or not with ATL disease progression."
34092254
sparser
"In turn, one may suppose that increased methylation of FHIT may simply reflect the fact of higher tumor cells in the samples collected."
34092254
sparser
"In conclusion, despite these limitations, our meta-analysis advocates that methylated SOX17 , CDO1 , ZFP42 , TAC1 , FAM19A4 , FHIT , MGMT , p16 , and RASSF1A are useful biomarkers in the screening and auxiliary detection of lung cancer."
28644424
sparser
"Smoking and alcohol ingestion may promote the methylation of FHIT and CDH13."
32474857
sparser
"Also, FHIT methylation could be developed into a rapid test, which would be extremely beneficial in screening asymptomatic carriers."
34092254
sparser
"Loss of FHIT mRNA expression correlated to FHIT promoter methylation but not to loss of heterozygosity at the 3p14 locus."
16513840
sparser
"Previous report showed that methylation of FHIT was absent in CIN but was frequent in invasive tumor."
28414756
sparser
"Unconditional logistic regression analysis was carried out to evaluate the capacity of MLH1 , ATM and FHIT gene methylation to predict recurrence."
25091577
sparser
"Results demonstrated FHIT methylation only in ATL patients’ nail samples (Fig. xref b)."
34092254
sparser
"C-myc suppresses miR-29 expression contributing to FHIT promoter methylation and loss in lung cancer cells and patient tumors with low miR-29 expression had a poor prognosis[ xref ]."
25910758
sparser
"Altogether, our results suggest that FHIT gene methylation observed in ATL samples is not the consequence of CIMP and support the possibility of a preexisting germinal epimutation."
34092254
sparser
"Among those 22, 20 showed methylation of the FHIT gene."
18299890
sparser
"FHIT methylation also was detected in 31% (16 of 51) of the primary BLs examined, including 2 samples whose derived cell lines also manifested FHIT hypermethylation."
15384174
sparser
"According to the results of the clinical features in this meta-analysis, we could also analyze the FHIT expression and FHIT gene methylation of liver tumor tissues in different stages."
28790842
sparser
"However, FHIT methylation has not been shown to be dependent upon patient age or gender in any other cancers [ xref – xref ] suggesting this is also the case in ATL."
34092254
sparser
"At present, FHIT and WWOX promoter methylation associated with the loss of gene expression has been reported for various types of cancers, including lung, breast, esophageal and bladder cancer ( xref )."
24137446
sparser
"Our study suggests that tumor-specific methylation of the p16, RASSF1A, H-cadherin, and RARbeta genes may be a valuable biomarker for the early detection of NSCLC in bronchial lavage, and that the age-related methylation of FHIT gene in the normal bronchial epithelium is related to the exposure to tobacco smoke."
15197197
sparser
"CONCLUSIONS The methylation of p16 and FHIT genes was an independent risk factor for hepatocellular carcinoma."
30773529
sparser
"Taken together, our results demonstrate that promoter aberrant methylation of FHIT is an important mechanism for inactivation of this tumor suppressor gene in many malignancies."
15574200
sparser
"Methylation of FHIT in HepG2 cells was detected by methylation-specific polymerase chain reaction (MSP)."
19728916
sparser
"It is also interesting to point out that there is a group of patients with a relatively low methylation of FHIT (<10%) but also negative staining of FHIT (Fig xref )."
25460508
sparser
"However, a more global question, is whether FHIT methylation inclines any individual to a wide array of cancers, not just ATL?"
35663592
sparser
"Significant differences were observed for the WWOX, FHIT and p73 methylation frequency between the ALL patients and controls (P<0.05)."
24137446
sparser
"The data clearly advocates that early testing of FHIT methylation status can lead to the determination of ATL leukemia risk assessment perhaps years or decades before the disease is initiated."
35663592
sparser
"The ORs only with HPV 16 positive (OR=2.58) or with serum folate level lower than 3.19ng/ ml (OR=1.28) in CIN group were all higher than the control status, but the OR only with FHIT methylation (OR=0.53) in CIN group was lower than the control status."
25422218
sparser
"The results suggest a potential role of FHIT methylation analysis in diagnosis and prognosis of lung cancer in clinical settings."
26796853
sparser
"Given that FHIT is found methylated in a small percentage of cells derived from PBMCs, there is a possibility that prior FHIT methylation may predispose individuals to leukemia/lymphomas."
35663592
sparser
"A study on age-related progression of FHIT methylation will need to be conducted in the future."
34092254
sparser
"Recently, in paired HNSCC primary A) and recurrent or metastatic (B) UMSCC-11A/11B, UMSCC-17A/17B, and UMSCC-81A/81B cell lines, using MS-MLPA, we identified nine genes, TIMP3, APC, KLK10, TP73, CDH13, IGSF4, FHIT, ESR1 , and DAPK1 that were aberrantly methylated in paired HNSCC primary A) and recurrent or metastatic (B) UMSCC-11A/11B, UMSCC-17A/17B, and UMSCC-81A/81B cell lines[ xref ]."
22388100
sparser
"These results suggest that aberrant methylation of the FHIT gene might be a key mechanism for silenced FHIT gene, which could be reactivated and whose tumor suppressing function could be restored by demethylating agent treatment."
16865265
sparser
"This strongly supports the idea that FHIT gene methylation can be used as a predictive biomarker for ATL disease progression."
35663592
sparser
"Our results extend the association between tobacco exposure and increased methylation of FHIT to breast tumor tissue."
30617699
sparser
"We then examined FHIT methylation status in corresponding family members (Fig. xref d)."
34092254
sparser
"There was a statistical statistically correlated with gender, and the methylation of FHIT and p16 genes (P&lt;0.05)."
30773529
sparser
"Among premenopausal women, compared with Stage 0 cases, cases of invasive cancer were more likely to have increased methylation of RARB (Stage I OR = 4.7, 95% CI: 1.1-19.0; Stage IIA/IIB OR = 9.7, 95% CI: 2.4-39.9; Stage III/IV OR = 5.6, 95% CI: 1.1-29.4) and lower methylation of FHIT (Stage I OR = 0.2, 95% CI: 0.1-0.9; Stage IIA/IIB OR = 0.2, 95% CI: 0.1-0.8; Stage III/IV OR = 0.6, 95% CI: 0.1-3.4)."
27245195
sparser
"The results indicated that folate was able to enhance apoptosis and inhibit the cervical cell proliferation while regulating FHIT gene methylation and expression."
29434897
sparser
"Folate deficiency and aberrant DNA methylation and expression of FHIT gene were associated with cervical pathogenesis."
29434897
sparser
"Methylation-specific polymerase (MSP) chain reaction was performed to detect the aberrant promoter methylation of FHIT gene in 55 patients with MDS."
18367246
sparser
"Methylation of FHIT was coordinated with methylation of SHP1 ( p =0.015)."
21592569
sparser
"These findings indicate that aberrant methylation of the FHIT gene is an independent adverse prognostic factor in MM."
16313263
sparser
"In the plasma of total 105 samples, 2 of 23 high grade intraepithelial neoplasia (HGIN) and 12 of 37 ESCC were detected p16 methylation, and 2 of 23 HGIN and 14 of 37 ESCC were detected FHIT methylation."
18365557
sparser
"We analyzed FHIT methylation using methylation-specific polymerase chain reaction and Fhit protein and acetylated histone H4 using immunohistochemistry in 60 resected tumor specimens."
16863736
sparser
"The incidence of FHIT and p16 methylation in the non-hepatoma group was 16.7% and 25.0%."
30773529
sparser
"We found a strong positive correlation between FHIT methylation in the plasma and the clinical stage and histological grade of the tumor."
16963412
sparser
"Patients with p16 methylation were 3.74 times [95% confidence interval (CI) = 1.62 - 7.95; P = 0.001] more likely to have FHIT methylation in squamous cell carcinoma."
15231689
sparser
"It remains highly unlikely due to several observations: 1- FHIT methylation was independent of proviral load, 2- non-infected cells (CD25- and CD19-) harbored methylated FHIT to the same extend as infected cells (arguing against the dilution effect), 3- gDNA extracted from nails was positive for FHIT methylation, and 4- the low number of FHIT methylated samples in asymptomatic carriers and TSP/HAM patients, who are infected with HTLV-I. We found approximately, 22% of acute and 11% of chronic ATL patient samples had unmethylated FHIT."
34092254
sparser
"In our case, FHIT promotor methylation was found in the anaplastic compo- nent of the dedifferentiated chondrosarcoma; there was no methylation in the cartilaginous tumor component."
12924447
sparser
"In addition, FHIT promoter methylation was correlated with clinical stage (advanced stage vs early stage: odds ratio = 2.69, p = 0.056) and tumor grade (high grade vs low grade: odds ratio = 4.11, p < 0.001)."
28639889
sparser
"The promoter region of FHIT gene is frequently methylated in non-small lung cancer cells and breast cancers, which correlates with the loss of expression of Fhit, and DNA methylation inhibitor treat[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23993961
sparser
"An inverse correlation has been shown between the methylation of RASSF1A , and FHIT and runt-related transcription factor 3 ( RUNX3 ) versus EGFR mutation ( xref )."
22989067
sparser
"5-Aza-dC can reverse the abnormal methylation of FHIT gene, activate the silenced gene and induce FHIT mRNA and protein expression in HepG2 cells."
19728916
sparser
"This is the first study indicating a correlation between the promoter methylation of FHIT and RASSF1A genes and the clinical stage and histological grading of cervical carcinoma in Indian women."
18608185
sparser
"According to our literature search, most studies have focused on the FHIT expression and FHIT gene methylation in Chinese population."
28790842
sparser
"Methylation of FHIT is also associated with poor survival ()."
23618899
sparser
"To determine if in rat tissues: (1) Fhit methylation status is related to expression levels and (2) Fhit methylation patterns were tissue- or carcinogen-specific, we examined Fhit methylation status and expression levels in DMBA- and MNU-induced benign and malignant mammary tumors."
15937960
sparser
"Therefore, studies would need to sample a significantly large cohort of normal tissue or cells to see if FHIT methylation is truly found in correspondingly healthy cells/tissue."
35663592
sparser
"Simultaneous methylation of the RASSF1 , APC, and FHIT genes was identified only in 3 patients with metastases."
27725787
sparser
"In support of this conclusion, the study demonstrated that in asymptomatic carriers, FHIT methylation was already present in patients that would develop ATL in less than 2 years’ time."
35663592
sparser
"These results suggest that in ATL families, 1- FHIT methylation is higher in descendants with methylated FHIT (FHIT methylation = 100%) than in normal asymptomatic carriers (FHIT methylation = 5.62%), 2- children of ATL parents have strongly methylated FHIT regardless of whether they are asymptomatic carriers or have ATL, 3- FHIT methylation may be passed down from parents to children through additive or non-additive genetic effects."
34092254
sparser
"No other gene was methylated in the benign tumors, whereas FHIT was methylated in 22%, RARB in 14.8%, BLU in 13.6%, p16INK4a in 11.1%, TNFRSF10C in 9.5%, and DAPK in 7.4% of ependymomas."
16616114
sparser
"For instance, increased methylation of FHIT was detected in bronchial lavage samples of cancer-free men and women who reported smoking for more than 40 pack-years ( xref )."
30617699
sparser
"In order to demonstrate the relationship between methylation of fragile histidine triad (FHIT) and T-cadherin/H-cadherin (CDH13) genes and liver cancer, the methylation status of FHIT and CDH13 was detected in healthy individuals and in Mongolian and Han patients with liver cancer."
32474857
sparser
"In contrast, FHIT was strongly methylated in the majority of samples isolated from HTLV-I ATL patients (Fig. xref a)."
34092254
sparser
"Detection of promoter methylation of FHIT has been identified by meta-analyses and demonstrated to be useful for the early diagnosis of breast and NSCLC carcinomas [ xref , xref ]."
33946400
sparser
"To test whether aberrant methylation of the FHIT gene may play a role in pediatric leukemia, we assessed the FHIT methylation status of 10 leukemia cell lines and 190 incident population-based cases of childhood acute lymphocytic and myeloid leukemias using methylation-specific PCR."
15026336
sparser
"Conclusions: The present meta-analysis demonstrates that methylated SOX17 , CDO1 , ZFP42 , TAC1 , FAM19A4 , FHIT , MGMT , p16 , and RASSF1A are potential superior biomarkers for the screening and auxiliary detection of lung cancer."
28644424
sparser
"Moreover, in the study we reported that direct descendants of ATL patients were found to possess a methylated FHIT gene, regardless of disease status."
35663592
sparser
"If so, even though this tissue presents as normal, FHIT methylation may already be present, suggesting that FHIT methylation may be one of the earliest markers for tumor development."
35663592
sparser
"The proliferation inhibition rate, apoptosis rate, and FHIT protein and mRNA expression levels increased along with rising concentrations of folate, whereas the degree of FHIT gene methylation gradually weakened in CaSki or C33A cell lines."
29434897
sparser
"The majority of acute and chronic ATL patient’s cells carry methylated FHIT, which is present in tumorigenic and non-tumorigenic cells, and leads to loss of FHIT expression."
34092254
sparser
"When compared to other viral lymphomas associated with infection by KSHV, HCV or EBV, ATL lymphoma patients presented with a higher proportion of methylated FHIT (Suppl xref ); however, the limited number of samples precludes this from being statistically significant and will need to be expanded upon in future studies."
34092254
sparser
"This data alone demonstrates that FHIT methylation represents a clear distinction between acute/chronic ATL disease and non-ATL/HTLV-I infected people."
35663592
sparser
"FHIT methylation was an early event, observed in one-third of the BLs from patients with or without lesions as well as in tumours."
16043258
sparser
"As expected, similar to OML1-R cells, FHIT expression (Fig. xref ) demonstrated a tight inverse correlation with promoter methylation of FHIT (Fig. xref ) in those cells."
25460508
sparser
"Methylated FHIT, MGMT, p16, and RASSF1A are underlying superior biomarkers, which can be used for lung cancer screening and auxiliary detection [ xref ]."
34257703
sparser
"Recently, using a multi-candidate gene approach, the Methylation Specific Multiplex Ligation-dependent Probe Amplification (MS-MLPA) assay (MS-MLPA, xref ) ( xref , xref ), we identified nine genes, TIMP3, APC, KLK10, TP73, CDH13, IGSF4, FHIT , ESR1 , and DAPK1 that were aberrantly methylated in paired HNSCC primary A) and recurrent or metastatic (B) UMSCC-11A/11B, UMSCC-17A/17B, and UMSCC-81A/81B cell lines ( xref – xref )( xref )."
22359294
sparser
"All these results further support the notion that in ATL patients, methylation of FHIT is not restricted to HTLV-I virus infected transformed ATL cells but rather methylated FHIT is present as a germinal alteration."
34092254
sparser
"Although FHIT and p15(INK4B) methylations were not correlated in MDS and AML, increased FHIT methylation at the relapse in AML was associated with p15(INK4B) methylation."
15902282
sparser
"By searching Pubmed, CNKI and Wanfang, the open published articles related to FHIT gene promoter methylation and lung carcinoma risk were collected."
24667261
sparser
"In contrast to a previous meta-analysis conducted by Yan et al . [ xref ], this study found that FHIT methylation was correlated with NSCLC in tissue samples, but this meta-analysis did not analyze whether FHIT methylation was linked to NSCLC in blood samples."
28404957
sparser
"For 93 adenocarcinomas there was no statistically significant association between FHIT methylation and age, gender, smoking history, pathologic stage and p16 methylation."
15231689
sparser
"Another group identified that promoter methylation of the fragile histidine triad ( FHIT ) gene in ESCC was significantly associated with exposure to tobacco smoke [ xref ]."
27110300
sparser
"FHIT, mutated in four out of five liver metastases, is believed to be a tumor suppressor involved in purine metabolism and lower FHIT expression/promoter methylation is associated with poor outcome in non-small cell lung and breast cancer and metastasis in colon cancer xref , xref , xref ."
28892047
sparser
"The level of aberrant methylation of FHIT , obtained in our study on cfDNA (55.6%), was close to the results obtained in the study of Kvasha et al. in samples of RCC tumors (54.6%)."
27725787
sparser
"Graphical representation and statistical analyses established that FHIT gene methylation in acute, chronic, and smoldering ATL subtypes, was statistically significant when compared to HD or asymptomatic samples (Fig. xref d)."
34092254
sparser
"Inverse correlation was found between EGFR mutation and FHIT, RASSF1A and RUNX3 methylation."
21508367
sparser
"Promoter methylation of p16, FHIT, RAR, CDH1, DAPK1, hTERT, RASSF1A, MGMT, BRCA1 and PALB2 genes was assessed by methylation-sensitive high-resolution melting (MS-HRM)."
25813726
sparser
"Aberrant promoter methylation of the FHIT gene was 34.15% (42/123) in cancer patients, but in none of the 105 controls."
20814237
sparser
"Maruyama et al. reported a significantly higher rate of FHIT gene methylation in prostate cancer tissues (15%) compared to nonmalignant tissues ()."
23618899
sparser
"At the same time, almost all cytosine nucleotides in the CpG dinucleotides were methylated (remained as cytosine after bisulfite modification) or partially methylated for all tumour samples with methy[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18378390
sparser
"As expected, the FHIT gene was methylated in all ATL patient samples, whereas CDKN1A and CDKN2A displayed much lower gene methylation (Fig.  xref a and b)."
34092254
sparser
"Multivariate analysis revealed that FHIT methylation, elevated beta-2-microglobulin serum levels and absence of auto-PBSCT from treatment were significant and independent prognostic factors in MM (Takada et al., 2005[ xref ])."
26417314
sparser
"Our data suggests that asymptomatic carriers that develop smoldering ATL have levels of FHIT methylation comparable to patients with smoldering ATL disease and confirm predictive power of methylated FHIT for disease progression."
34092254
sparser
"The purpose of this study is to conduct a meta-analysis and literature review to investigate the clinicopathological significance of FHIT methylation in BC."
26491255
sparser
"In this study, we investigated the possible association of promoter methylation of PCDH17, NEFH, RASSF1A, and FHIT, genes with the prognosis of nonmetastatic RCC patients."
35838992
sparser
"Wu et al [ xref ] reported that FHIT was highly methylated in Hela, C33A and Siha cervical cancer cells, and that aberrant methylation of the FHIT gene might be a key mechanism for cervical tumorigenesis, which could be reactivated and whose tumor suppressing function could be restored by treatment of demethylating agent."
21999220
sparser
"Importantly, patients with CD with FHIT methylation diagnosed over a 5-year period exhibited poor outcomes suggestive of severe disease behavior and an increased risk of disease."
32375395
sparser
"Our main findings are as follows: (a) methylation was completely absent in control tissues; (b) the frequencies of methylation for all of the genes except hMLH1 were >20% in cervical cancers; (c) aberrant methylation commenced early during multistage pathogenesis and methylation of at least one gene was noted in 30% of the nondysplasia/low-grade CIN group; (d) an increasing trend for methylation was seen with increasing pathological change; (e) methylation of RARbeta and GSTP1 were early events, p16 and MGMT methylation were intermediate events, and FHIT methylation was a late, tumor-associated event; and (f) methylation occurred independently of other risk factors including papillomavirus infection, smoking history, or hormone use."
11297252
sparser
"Methylation of the FHIT gene was more frequent in children with t ( xref ; xref ), and with good response to prednisolone."
21592569
sparser
"Analysis of FHIT Promoter Methylation in CRC."
23573237
sparser
"The promoter methylation of FHIT and ESR1 showed an increase from about 34% to 53% and 32% to 45%, respectively."
23691160
sparser
"RESULTS The incidence of FHIT and p16 methylation in serum from the liver cancer group was 51.8% and 67.9%, respectively."
30773529
sparser
"Increased methylation in DAPK1, RARB, TIMP3, CCNA, and FHIT is associated with cervical cancer and low or no methylation is observed in LSHL lesion and normal cytology [ xref – xref ]."
27933097
sparser
"The above consistencies and inconsistencies between the three studies implied the need to test the association between FHIT methylation and NSCLC with larger sample sizes and more advanced technology."
28036263
sparser
"The promoter methylation of DAPK1, FHIT, MGMT, and CDKN2A in cervical cancer vs. intraepithelial neoplasia vs. normal cervical tissue was 75.5 vs. 31.8 vs. 4.2 % (p < 0.0001), 66.0 vs. 59.1 vs. 25.0 % (p = 0.0033), 34.0 vs. 27.3 vs. 20.8 % (p = 0.76), and 17.0 vs. 31.8 vs. 8.3 % (p = 0.11), respectively."
23494221
sparser
"FHIT gene methylation was also reported in serum of sporadic breast cancer in a CpG island methylator phenotype (CIMP) screening [ xref ]."
22295218
sparser
"Haroun et al observed that the methylation frequencies of the genes tested in non-small cell lung carcinoma specimens were 53.6% for FHIT . xref Jeong et al evaluated the methylation of FHIT in 60 BC samples; FHIT methylation was detected in 96.7% and the positive expression rate of FHIT in 87.3% of the patients. xref Our data and previous studies indicate that FHIT promoter hypermethylation is a useful diagnostic biomarker of BC."
26491255
sparser
"If this is the case, FHIT methylation could help determine if surrounding tissue (which macroscopically appears normal) may be susceptible to transformation and the spreading of the tumor."
35663592
sparser
"Hypermethylation of FHIT gene promoter region was found more frequent in cancer tissue than that of NLT which may demonstrated association between lung cancer risk and FHIT gene promoter methylation."
24667261
sparser
"WWOX and FHIT promoter methylation is detected in tissue adjacent to breast cancer and WWOX exon 1 MSP distinguishes breast cancer DNA from DNA of adjacent and normal tissue."
15674328
sparser
"Since genetic alterations were not frequently observed in DNA corresponding to the FHIT gene in renal tumours, to elucidate the mechanism of FHIT gene silencing we examined 22 paired samples of clear cell renal carcinoma and non-malignant renal tissue for the methylation of the FHIT 5'CpG island by methylation-specific PCR."
18378390
sparser
"In thyroid cancer, the inactivation of FHIT was suggested to be associated with the pathogenesis of thyroid neoplasm xref and the homozygous deletion and promoter methylation of FHIT was reported to be associated with DTC xref xref ."
28703219
sparser
"For example, in patients with acute leukemia, which compared with the dual vacuity of Qi and Yin syndrome, ID4 gene methylation is more likely to occur in patients with toxic hot flaming syndrome and static blood and binding phlegm syndrome.[ xref ]Differences in methylation of HLA-DRB1 and ADAMTS9, NUDT1 and YES1, and APOA5 and PRKCZ genes might be related to hepatitis B and cirrhosis with damp-heat brewing internally syndrome, spleen deficiency, and exuberant phlegm and liver-kidney yin depletion.[ xref ]The methylation of the promoter regions of FHIT, MAP2K6, WNT5B, CSNK1D, and FRAT2 genes on CpG island was associated with kidney yang vacuity.[ xref ]At present, the research of molecular mechanism of DNA methylation is still at the initial stage."
30278525
sparser
"These results suggested that FHIT methylation was accumulated through the disease progression of MDS and AML, and the role of the FHIT gene as a tumor suppressor seemed different in AML and MDS."
15902282
sparser
"Methylation of 5'-CpG islands in 10 normal cervical squamous epithelial tissues, and 40 cervical cancer tissues was detected with methylation specific polymerase chain reaction (MSP), protein expression of FHIT was detected with immunohistochemistry, and their correlations with clinicopathologic features of cervical cancer were statistically analyzed. (1) The 5'-CpG islands methylation rate of FHIT gene in cervical cancer tissues was 40.0% (16/40), while no methylation of FHIT gene was found in normal cervical tissues. (2) The methylation rates of FHIT gene in cervical cancer of stage I was 14.3% (2/14), significantly lower than that in cervical cancer of stage II (56.5%, 13/23) (P<0.05). (3) Expression of FHIT protein in cervical cancer was 33.0% (12/40), significantly lower than that in normal cervical tissue (100%, 10/10) (P<0.05)."
15642192
sparser
"Another possibility is that perhaps the “normal” tissue, outside the surgical margins, is truly FHIT methylation positive, and not due to contamination from the surrounding lesion."
35663592
sparser
"Currently, no clinical test for FHIT methylation exists."
35663592
sparser
"The hazard of failure after esophagectomy for stage 1-2 cancers with FHIT methylation was about 5.81 (95% CI = 1.15-14.07; P = 0.009) times higher than in those without."
16564166
sparser
"Methylation of FHIT commences during lung cancer pathogenesis and may represent a marker for risk assessment."
11325823
sparser
"Additional studies with larger subjects are necessary to further assess the role of FHIT methylation in blood samples of patients with NSCLC."
28404957
sparser
"P16 INK4 , FHIT, and E-cadherin were methylated in the highly malignant osteosarcomatous compartment of the tumor."
12924447
sparser
"Aberrant promoter methylation of the FHIT gene was found in 28.3% (17/60) of cases and of the RASSF1A gene in 35.0% (21/60) of cases; promoter methylation of both the genes was found in 13.3% (8/60) of cervical cancer cases."
18608185
sparser
"Our study suggests that FHIT promoter methylation may be an independent prognostic biomarker in early stage esophageal squamous cell carcinoma."
16564166
sparser
"The gel images of WWOX, FHIT and p73 methylation are shown in xref – xref ."
24137446
sparser
"We found a relationship between APC methylation and good histological differentiation (p =0.03); CDH1 methylation with diffuse type by Lauren and 3 or more metastasic lymph nodes (p <0.05); FHIT, CDKN2A and CDH1 methylation and female condition (p <0.04)."
16163424
sparser
"We also found that longitudinal HTLV-I carriers that progressed to smoldering ATL and descendants of ATL patients harbor FHIT methylation."
34092254
sparser
"Strikingly, the FHIT gene was methylated in the vast majority (71%, 147 out of 207) of patients with CD samples ( xref b, right panel; xref )."
32375395
sparser
"Similar reports have been reported for the FHIT methylation in highly malignant osteosarcomatous [ xref ]."
15574200
sparser
"SHS exposure after age 61 and active smoking between ages 41 to 50 was associated with increased methylation of FHIT ."
30617699
sparser
"Kras gene mutation at codon 12 & 13 and methylated RASSF1A, FHIT and MGMT gene was observed in 47%, 19%, 47%, 37% and 47% cases, respectively."
23573237
sparser
"There appeared positive additive interaction between FHIT methylation, FHIT protein low expression and infection of HPV16."
27346116
sparser
"There was more FHIT promoter methylation in cervical cancer than in cervical intraepithelial neoplasia lesions and normal controls (odds ratio = 8.0, p = 0.055; odds ratio = 10.75, p < 0.001, respectively)."
28639889
sparser
"Comprehensive methylation profiling identified relationships between methylation of FHIT and ANKRD11, which have been previously linked to neurodevelopment and behavioral outcomes in genetic association studies."
27366929
sparser
"However, due to the small sample size, we only observed a trend that patients with higher FHIT methylation tend to have a 2-fold lower locoregional control rate than that of patients with lower methylation (survival%: high vs low methylation, 33.9% vs 66.7%, P = 0.0998)."
25460508
sparser
"These results indicate that p16 and FHIT methylation may be one of the earliest events and an important mechanism for gene silencing in esophageal squamous cell carcinogenesis."
18365557
sparser
"While loss of FHIT expression can occur due to chromosome breaks, FHIT is also highly methylated in solid tumors, such as lung, breast, bladder, prostate, cervical, esophageal, and hepatocellular cancers [ xref ]."
34092254
sparser
"However, because matched DNA could not be obtained for all HD and ATL patient samples, an analysis against FHIT methylation could not be made."
34092254
sparser
"We then compared the mean methylation levels of gene promoters in R and NR patients, confirming that MLH1, ATM and FHIT were significantly differentially methylated in adenomas on the basis of the presence or not of lesion recurrence (Figure  xref )."
25091577
sparser
"This is remarkable, given that ACs have low FHIT methylation (5.62%)."
34092254
sparser
"Overall, the present findings demonstrated that the FHIT gene was methylated in F6 cells and demethylation treatment lead to changes in the biological characteristics, thereby promoting the apoptosis of F6 cells."
26137124
sparser
"Recently, using a multi-candidate gene assay, xref we identified nine genes, TIMP3, APC, KLK10, TP73, CDH13, IGSF4, FHIT , ESR1 , and DAPK1 that were aberrantly methylated in paired HNSCC primary and recurrent or metastatic cell lines xref The most frequently hypermethylated genes were APC and IGSF4 observed in 3/6 cell lines, and TP73 and DAPK1 observed in 2/6."
20620629
sparser
"In the absence of nicotine, methylation of the FHIT gene and attenuation of Fhit protein were not detected in HEECs."
16570269
sparser
"RESULTS of the present study suggest that methylation of FHIT is a useful biomarker of biologically aggressive disease in patients with NSCLC."
24935385
sparser
"A study found FHIT methylation in non-neoplastic adjacent tissue in 8.3% of lung and 14% of breast tissue [ xref ]."
35663592
sparser
"Again, all family members demonstrated strong FHIT methylation, including the AC son."
34092254
sparser
"The inconsistent readings between studies and the high levels of FHIT methylation in normal tissues deriving from these solid tumors could be due to contamination with surrounding, tumorigenic tissue."
35663592
sparser
"In this commentary we discuss the importance of developing diagnostic tools for the early detection of FHIT methylation and the possibility that prior FHIT methylation may predispose any individual to the development of cancer."
35663592
sparser
"Concordance rates of 75% and 81.7% were present between promoter methylation of FHIT and expression of Fhit (p = 0.035) and acetylated H4 (p = 0.02)."
16863736
sparser
"In contrast, it has been demonstrated that aberrant methylation of FHIT occurred specifically in lung and breast cancers [ xref ]."
16928264
sparser
"FHIT methylation also did not correlate with the viral load of the patient, meaning that even in acute and chronic ATL patients with low HTLV-I proviral loads, FHIT methylation was still present."
35663592
sparser
"FHIT gene DNA methylation showed all positive at the folate concentration levels of 1 µg/ml and 10 µg/ml, partially positive at 100 µg/ml and 250 µg/ml, but negative at 500 µg/ml and 1 000 µg/ml in both C33A and CaSki cells."
25059370
sparser
"In addition, we used a large number of patients with CD ( n = 207) to validate the methylation of the fragile histidine triad ( FHIT ) gene."
32375395
sparser
"We conclude that methylation of FHIT is a frequent event in NSCLC and breast cancers and is an important mechanism for loss of expression of this gene."
11325823
sparser
"We used methylation-specific PCR to analyze promoter methylation of the p16 and FHIT genes at baseline and 3 months after intervention in 1,774 oral and bronchial brush specimens from 127 smokers enrolled in a randomized placebo-controlled chemoprevention trial."
19138934
sparser
"IGSF4 , DAPK1 and FHIT were aberrantly methylated in 3 of 13 cell lines with concordant loss of expression for IGSF4 in UT-SCV-4, which supported promoter hypermethylation of both copies."
19047905
sparser
"The FHIT methylation was found in 13 of 94 (13.8%) AML and 22 of 40 (55.0%) MDS cases, but not in normal mononuclear cells (MNCs)."
15902282
sparser
"Our results show that nicotine induces methylation of the FHIT gene followed by loss of Fhit protein expression in HEECs."
16570269
sparser
"The difference is possibly due to the diagnosis time for ALL, which leads to the alteration of FHIT methylation frequency, as numerous studies have demonstrated that FHIT methylation is an accumulated process and different diagnosis times exhibit different methylation frequencies ( xref – xref )."
24137446
sparser
"Koga et al. reported that EGFR mutation and CHFR hypermethylation in lung adenocarcinoma are mutually exclusive [ xref ], Similarly, Suzuki et al. reported that lung tumors with wildtype EGFR show more prevalent hypermethylation of p16 (34% vs. 16%) and SPARC (60% vs. 37%) genes compared to EGFR mutant tumors [ xref ], Yanagawa et al. also compared the relationship between methylation of 10 cancer-related genes and mutations of EGFR, KRAS, and TP53 in 62 lung adenocarcinoma cases from smokers and never-smokers and found that the prevalence for methylation of FHIT, RASSF1A, and RUNX3 genes in EGFR mutant tumors was significantly lower compared to EGFR wild-type tumors , In contrast, the significantly higher rate of TOX3 methylation in EGFR mutant than wildtype tumors demonstrates that aberrant methylation is not always inversely associated to EGFR mutation."
30089603
sparser
"Does pre-existing FHIT methylation contribute to tumorigenesis?."
35663592
sparser
"The odd ratio (OR) for FHIT methylation in cancer group was 3.43 (95% CI: 1.85 - 6.36) in random effects model, and 2.03 (95% CI: 1.60 - 2.57) in fixed effects model, indicating a slight increase of methylation in lung cancer tissues (Figure xref )."
28036263
sparser
"However, FHIT methylation in 125 squamous cell carcinomas was associated with exposure to tobacco smoke and p16 methylation, but not with age, gender and pathologic stage."
15231689
sparser
"Furthermore, we found that aberrant promoter methylation of the FHIT gene showed a highly significant association with clinical stage I and not with clinical stage IV in lung cancer (p < 0.05)."
20814237
sparser
"Compared with those who had smoked less than 20 pack-years, those who had smoked greater than 40 pack-years were at a 13.10-fold increased odds of having methylation of FHIT (95% CI: 4.94; 63.96)( xref )."
30617699
sparser
"Once again, the comparable amount of CpG islands methylated in CD19- and CD19+ cell fractions support a germinal origin of methylated FHIT."
34092254
sparser
"P16 and FHIT methylation was reversely correlated with protein expression in EBVaGC."
25720522
sparser
"FHIT methylation may serve as the initial event that triggers cellular transformation."
35663592
sparser
"Promoter methylation of the LRRC3B gene was detected in 20 out of 27 samples (74%); methylation of the RASSF1, APC , and FHIT genes was found in 17 (63%), 14 (52%), and 15 (55.6%) patients, respectively (see xref for detailed methylation frequencies)."
27725787
sparser
"Concordance analysis was performed between FHIT methylation and expression of Fhit as well as H4."
16863736
sparser
"Multivariate analysis identified FHIT gene methylation [hazard ratio (HR) 1.722, 95% confidence interval (CI) 1.150-2.603, P = 0.009], elevated beta-2-microglobulin serum levels (>6.5 mg/L, HR 2.005, 95% CI 1.035-3.937, P = 0.004), and treatments not including auto-PBSCT are independent predictive variables."
16313263
sparser
"As described in our study, pre-existing FHIT methylation predisposes HTLV-I carriers to the development of ATL ( xref )."
35663592
sparser
"FHIT methylation serves as a robust biomarker signature for the progression of acute and chronic ATL disease."
35663592
sparser
"FHIT methylation in 257 primary esophageal squamous cell carcinomas was retrospectively analyzed by methylation-specific polymerase chain reaction."
16564166
sparser
"The FHIT methylation was found to be significantly associated with exposure to tobacco smoke (P = 0.007) and with a poor prognosis in cases of stage 1-2 cancer irrespective of recurrence."
16564166
sparser
"Nicotine induced the methylation of FHIT gene and attenuated Fhit protein in association with increased expression of DNMT3a."
16570269
sparser
"Using bisulfite treatment followed by MS-PCR we detected methylation of the LRRC3B, APC, FHIT, and RASSF1 genes in the cfDNA of cancer patients."
27725787
sparser
"For instance, Lin et al found that the frequency of FHIT gene hypermethylation in myelodysplastic syndrome patients was higher than in normal persons. xref Moreover, it has been reported that the loss of FHIT was associated with the risk of gastric cancer, breast cancer, and lung cancer. xref – xref And the aberrant methylation of FHIT gene had a significant association with the clinical stage and other clinical features."
28790842
sparser
"The presence of very low level of FHIT expression in tumours with methylated FHIT 5′CpG island might be due to residual background contamination of tumour samples with normal stromal cells."
18378390
sparser
"To date, there have been some studies describing the methylation status of FHIT in NSCLC; however, the roles of methylation of FHIT in NSCLC and clinical significance have not been thoroughly investigated."
26796853
sparser
"However, our study provides clinically relevant evidence that the methylation of FHIT might be correlated with CD clinical parameters."
32375395
sparser
"Promoter methylation of the CDH1 , RARB , FHIT , and MGMT genes was noted only in SCC, whereas the AC exhibited significantly higher frequency of promoter methylation of HIC1 (60% in AC vs. 15.6% in SCC), APC (80% in AC vs. 6.5% in SCC), and BRCA1 (40% in AC vs. 3.9% in SCC) (Table xref )."
12773202
sparser
"We have also used an epigenome-wide scan of DNAm in the placenta to demonstrate relationships between methylation of the FHIT and ANDKR11 genes, which had been previously linked to neurodevelopmental and behavioral outcomes, and performance on the NNNS attention scale in a cohort of term newborns xref ."
31004082
sparser
"Although no association between FHIT gene methylation and clinical variables was found, the estimated median survival of the methylated group was significantly shorter than that of the unmethylated group."
26417314
sparser
"Methylation-specific PCR (MSP) was used to detect FHIT promoter methylation in bone marrow samples from 54 MDS cases."
18247301
sparser
"FHIT methylation is known to cooperate with the p16 methylation in the progression of LC [ xref ]."
24238736
sparser
"Paquette and colleagues found that DNA methylation of FHIT and ANKRD11 , associated with brain and neurobehavioral development and placental physiology, were related to infant attention."
30068429
sparser
"Among patients with ATL disease acute (74.2%) and chronic (88.6%) ATL patient samples were overwhelmingly represented by FHIT gene methylation, whereas 45 and 20% of smoldering and lymphoma-type ATL patient samples were found to have methylated FHIT."
34092254