IndraLab
Statements
reach
"To substantiate the in vivo stabilization of HIF-1alpha by HAUSP, a transient co-transfection experiment showed that overexpression of HAUSP increased HIF-1alpha at the protein levels but not the mRNA levels (XREF_FIG and XREF_SUPPLEMENTARY), and further activated a Twist1 promoter driven reporter construct (XREF_FIG) XREF_BIBR."
reach
"Finally, co-immunoprecipitation experiments using anti-HIF-1alpha antibodies to pull down VHL and components of the E3 ubiquitin ligase complex showed that HAUSP knockdown did not affect the interaction between HIF-1alpha and VHL or the components of the E3 ubiquitin complex (XREF_SUPPLEMENTARY), indicating that the ability of HAUSP to increase HIF-1alpha stability was independent of its interaction with VHL or the components of the E3 ubiquitin ligase complex."