IndraLab

Statements

USP11 binds SPRTN. 9 / 9
| 2 7
sparser
"We next confirmed SPRTN-USP11 interaction by immunoprecipitation in HEK 293T cells expressing either SFB-USP7, SAMHD1, or SPRTN."
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sparser
"Moreover, we observe SPRTN interaction with USP11 in the absence and upon DNA damage."
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sparser
"Based on our observation that SPRTN and USP11 interact ( xref and xref ) and because USP11 is a deubiquitinase ( xref , xref , xref ), we investigated whether SPRTN is a substrate for USP11 deubiquitinase."
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sparser
"We observed a slight increase in SPRTN-USP11 interaction following treatment with etoposide (VP16), a TOP2 crosslinking agent ( xref D , xref ), camptothecin (CPT), TOP1 cross-linking agent, formaldehyde (nonspecific cross-linking agent), and hydroxyurea (non-cross-linking agent) ( xref )."
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sparser
"To gain insight into the functional relevance of SPRTN-USP11 interaction, we first examined whether USP11 functions in response to DNA damage caused by DPC-inducing agents."
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sparser
"Co-immunoprecipitation (Co-IP) of Myc-USP11 with SFB-SPRTN full-length (FL) or ΔSprT, ΔSH, ΔPIP, ΔUBZ, E112A catalytic inactive, and Y117C (SPRTN mutation identified in RJALS patients) mutant constructs showed that SPRTN-USP11 interaction was lost with deletion of the N-terminal SprT domain of SPRTN ( xref A )."
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reach
"VCPIP1 and USP11 interact weakly with SPRTN in co-immunoprecipitating experiments, but show no (VCPIP1) or only weak (USP11) preference for SPRTN-Ub (XREF_SUPPLEMENTARY)."
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reach
"USP11 interacts with SPRTN and cleaves monoubiquitinated SPRTN in cells and invitro."
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sparser
"USP11 interacts with SPRTN and cleaves monoubiquitinated SPRTN in cells and in vitro ."
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