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Statements

Magnesium atom activates KCNMA1. 20 / 20
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"Mg 2+ i activates BK channels by binding to low-affinity sites and can also modulate BK channel activity through inhibitory action on the high-affinity sites."
17001085
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"Our results show that intracellular Mg 2+ activates Slo1 BK channels through a fundamentally different mechanism from Ca 2+."
18166624
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"Intracellular Mg 2+ Enhances BK Channel Function at Physiological Conditions."
11696614
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"BK (Slo1) potassium channels are activated by millimolar intracellular Mg 2+ as well as micromolar Ca 2+ and membrane depolarization."
18166624
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"Fig. 5 A shows the comparison of Mg 2+ -dependent activation of the WT mSlo1 channels with that of mutant channels Q397R, Q397K, Q397D, Q397E, Q397C, and Q397W."
16877509
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"Moreover, in absence of Ca 2+ , BK channel-activating concentrations of Mg 2+ (10 mM) did not support CLR action."
34969832
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"Our results illustrate that Mg 2+ is coordinated at the interface between the VSD and the cytoplasmic domain to activate Slo1 BK channels."
18931675
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"The mechanism of Mg 2+ activation of the BK channel was thought to be due to a modulation of the voltage dependent BK channel activation XREF_BIBR, XREF_BIBR, XREF_BIBR."
24489780
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"Thus, for most alcoholics the low levels of internal Mg 2+ would contribute to tolerance of the EtOH induced potentiation of BK channel activity."
26331878
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"It has often been reported that Ca 2+ and Mg 2+ not only activate MaxiK channels but also block at millimolar concentrations."
17591987
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"In addition to Ca 2+, millimolar intracellular Mg 2+ can activate Slo1 channels."
29025867
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"The bound Mg 2+ at this narrow interdomain interface activates the BK channel through an electrostatic interaction with a positively charged residue in the VSD."
23359284
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"Thus, high concentrations of Ca 2+ and Mg 2+ may be acting at the same site (s) on the channel protein to modulate BK channel gating, independent of the action of Ca 2+ at the higher affinity, Ca 2+ -specific sites."
11696615
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"If we assume that high concentrations of Ca 2+ and Mg 2+ bind to the same or similar sites and are able to promote activation of Slo1 currents in a similar fashion, then the results in XREF_FIG A can be used to " subtract " the effect of Ca 2+ at the putative low affinity site from the V 0.5 -pCa relationship shown in XREF_FIG E."
11696615
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"The ability of Mg 2+ and other divalent cations to potentiate BK channel activation by Ca 2+ is associated with an increase in the Hill coefficient for activation by Ca 2+ (Golowasch et al. 1986; Oberhauser et al. 1988)."
11696615
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"In 2001, two independent studies of the cloned BK channel illustrated that millimolar Mg 2+ activates the BK channel via a low-affinity metal binding site that is independent from Ca 2+ dependent activation [XREF_BIBR, XREF_BIBR]."
19099186
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"Remarkably, this requirement was divalent-specific: in the absence of Ca , BK channel-activating concentrations of Mg (10 mM) did not support cholesterol-induced inhibition of BK current [69]."
36988883
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"Mg 2+, which enhances BK channel activation through the E399 low affinity site, only contributes to a slowing of deactivation of Slo1, with little effect on the activation kinetics of channel gating (XREF_FIG A)."
15738049
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"Additional support for a secondary site are the observations of Solaro et al. 1995 that cytoplasmic Mg 2+ modulates Ca 2+ -dependent activation of mSlo1 by binding to a low affinity site on the channel core, and the observations of Shi and Cui 2001 that Mg 2+ can increase BK channel activity under experimental conditions (1 mM Ca 2+ i), where the primary Ca 2+ binding sites would be saturated with Ca 2+ (Rothberg and Magleby 1999)."
11723163
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"Quantitative computation of these effects reveals that the overall effect of Mg 2+ under physiological conditions is to enhance BK channel function."
11696614