IndraLab

Statements

KCNQ1 binds KCNQ1OT1. 9 / 9
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"Collectively, our data from these three distinct models demonstrate an allele-specific interaction between the Kcnq1ot1 lncRNA and KvDMR1– Kcnq1 promoter DNA regions."
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"We detected the direct interaction of Kcnq1ot1 noncoding RNA (Ra and Rb sites) with both the Kcnq1 promoter (D1 and D2 sites; xref , left, lanes 1 and 2) and the Kcnq1 ICR (KvDMR1; D5 and D6 sites; xref , right, lanes 5 and 6)."
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"Using this SNP to separate the allelic R3C products, we found that Kcnq1ot1 lncRNA interacted exclusively with the paternal Kcnq1 promoter ( xref , bottom, lanes 1 and 2)."
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"Using R3C, we show that in MBW2 control cells, Kcnq1ot1 directly interacted with the Kcnq1 promoter ( xref , left, lanes 1 and 2)."
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"In both the wild-type cells and in cells in which the deletion was maternally inherited ( xref , −(M)/+), we detected interactions of Kcnq1ot1 noncoding RNA with the Kcnq1 promoter DNA ( xref , D2/Rb) and with KvDMR1 ( xref , left, lanes 1–3, D5/Rb)."
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"Using R3C, we show that in both the wild-type and control shRNA cells (shK1, shH19, and shNespas), Kcnq1ot1 directly interacted with the Kcnq1 promoter ( xref , D2/Rb) and KvDMR1 ( xref , D5/Rb)."
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"Because Kcnq1ot1 lncRNA interacts with both the Kcnq1 promoter and the KvDMR1, which are 200 kb apart, we hypothesized that the Kcnq1ot1 lncRNA scaffolds these long-distance DNA regions to form an intrachromosomal structure, which is critical for the maintenance of allelic expression of genes in this imprinted region."
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"Using two complementary approaches, we demonstrate that Kcnq1ot1 lncRNA interacts directly with chromatin to form a 200-kb intrachromosomal loop between the downstream Kcnq1 promoter and the KvDMR1where the Kcnq1ot1 promoter is located."
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"All eight imprinted domains captured in this study, namely Peg10 - Sgce , Mest - Copg2 , Peg3 - Usp29 , H19 - Igf2 , Kcnq1-Kcnq1ot1 , Snrpn - Peg12 , Meg3 - Dio3 and Peg13 - Kcnk9 , exhibited pronounced allele-specific chromatin structures ( xref – xref )."
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