IndraLab

Statements

NPM1 is phosphorylated on T237. 11 / 11
2 | 5 4
hprd
No evidence text available
20068231
hprd
No evidence text available
17287340
rlimsp
"Upon analysis, we found phosphorylated level of nucleophosmin (NPM) at Threonine 234/237 (p-NPM-Thr234/237) had remarkably high level in metastatic HCC cells (PLC-LM) than the corresponding primary HCC cell line (PLC-PT)."
26536659
sparser
"Accompanied with the reduction in CDK1 expression, p-NPM-Thr 234/237 was consistently suppressed in two shCDK1 clones when compared to NTC control, which suggesting that CDK1 is upstream kinase leading to NPM phosphorylation at Thr234 and Thr237 (Figure xref )."
26536659
sparser
"Since NPM is phosphorylated on Thr234 and Thr237 by cyclin-dependent kinase (CDK) 1/cyclinB [ xref ], we tested whether knockdown of CDK1 had a role in the regulation of p-NPM-Thr 234/237 mediated HCC metastasis."
26536659
sparser
"The only exception was NPM1, which was exclusively phosphorylated at Thr237 but not Ser70 using recombinant protein, in contrast to the results from crude extracts."
35291876
rlimsp
"To determine the functional significance of p-NPM-Thr234/237 in HCC, we first performed proliferation assay to compare the growth properties of NPM and its phosphorylation site mutant."
26536659
sparser
"CDK-1 mediated phosphorylation of NPM1 at T199, T219, T234 and T237 has been shown to play a critical role in mitosis [42] ."
29157973
rlimsp
"By monitoring the phosphorylation of endogenous NPM immunoprecipitated from cells, we found that purified recombinant PP1 is capable of removing phosphate groups on the Thr199 and Thr234/237 residues of NPM (Figure 1C and Supplemental Figure S2C)."
20962268
rlimsp
"Total NPM1 expression did not appear to change upon N6L treatment neither with time of treatment nor with the concentration used whereas a substantial decrease in NPM1 phosphorylation was observed with 20 μmol/L N6L (63% for Thr199 and 86% for Thr234/237 of reduction at 48 hours) (Figure 2A and 2B)."
26993766
sparser
"Biochemical and immunocytochemistry analyses verified that the phospho-MKK1/MKK2 antibodies cross-reacted with NPM that was phosphorylated at Thr234 and Thr237 during G2/M-phase, which are the same sites that are targeted by Cdc2 (cell division cycle protein-2) during mitosis."
14670079