IndraLab

Statements

NPM1 is phosphorylated on T237. 16 / 16
2 | 6 8
rlimsp
"Additionally, mutations in the NPM1 gene were introduced which made the protein phosphorylation-insensitive at serine-4, serine-125, theonine-199, or threonine-234/237, respectively. Contrary to our expectations, we could not see any relocation of NPM1 from the nucleoli into other parts of the cell when the individual phosphorylation sites were “turned off.."
30453269
rlimsp
"NPM1 is dephosphorylated stepwise within 1 hour after irradiation at two of its major phosphorylation sites: threonine-199 and threonine-234/237."
30453269
sparser
"The only exception was NPM1, which was exclusively phosphorylated at Thr237 but not Ser70 using recombinant protein, in contrast to the results from crude extracts."
35291876
sparser
"Biochemical and immunocytochemistry analyses verified that the phospho-MKK1/MKK2 antibodies cross-reacted with NPM that was phosphorylated at Thr234 and Thr237 during G2/M-phase, which are the same sites that are targeted by Cdc2 (cell division cycle protein-2) during mitosis."
14670079
rlimsp
"Phosphorylation Pattern of NPM1 at Serine-4, Serine-125, Threonine-199, and Threonine-234/237 within 1 Hour after Irradiation."
30453269
rlimsp
"Total NPM1 expression did not appear to change upon N6L treatment neither with time of treatment nor with the concentration used whereas a substantial decrease in NPM1 phosphorylation was observed with 20 μmol/L N6L (63% for Thr199 and 86% for Thr234/237 of reduction at 48 hours) (Figure 2A and 2B)."
26993766
sparser
"Since NPM is phosphorylated on Thr234 and Thr237 by cyclin-dependent kinase (CDK) 1/cyclinB [ xref ], we tested whether knockdown of CDK1 had a role in the regulation of p-NPM-Thr 234/237 mediated HCC metastasis."
26536659
sparser
"Accompanied with the reduction in CDK1 expression, p-NPM-Thr 234/237 was consistently suppressed in two shCDK1 clones when compared to NTC control, which suggesting that CDK1 is upstream kinase leading to NPM phosphorylation at Thr234 and Thr237 (Figure xref )."
26536659
sparser
"In particular, there were marked decreases in the phosphorylation of nucleophosmin at Thr-199, Thr-234 and Thr-237 ( Tarapore et al., 2006 ), which are catalyzed by cyclin-dependent kinase (CDK)1 and [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17166628
rlimsp
"This revealed that NPM1 phosphorylation at threonine-199 and threonine-234/237 was more prominent in the cytoplasm than in the nucleus in both cell lines."
30453269
rlimsp
"Upon analysis, we found phosphorylated level of nucleophosmin (NPM) at Threonine 234/237 (p-NPM-Thr234/237) had remarkably high level in metastatic HCC cells (PLC-LM) than the corresponding primary HCC cell line (PLC-PT)."
26536659
rlimsp
"Thus, we analyzed the relative phosphorylation of NPM1 in the nucleus and in the cytoplasm at serine-4, serine-125, threonine-199, and threonine-234/237 in HeLa and in A549 cells (Figure 6)."
30453269
sparser
"CDK-1 mediated phosphorylation of NPM1 at T199, T219, T234 and T237 has been shown to play a critical role in mitosis [42] ."
29157973
rlimsp
"By monitoring the phosphorylation of endogenous NPM immunoprecipitated from cells, we found that purified recombinant PP1 is capable of removing phosphate groups on the Thr199 and Thr234/237 residues of NPM (Figure 1C and Supplemental Figure S2C)."
20962268
hprd
No evidence text available
20068231
hprd
No evidence text available
17287340