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EGFR affects EGF
16 2 12 6 | 11 138 1055 847
16 2 12 6 | 11 138 1055 847
sparser
"We present a model for how high-affinity EGF binding to the EGF receptor may remove the dimerization arm from its intramolecular binding site, thus promoting receptor dimerization and activation."
sparser
"The current data show that GCN4-ICM forms multimers, because the cross-correlation value for this construct,f c = 0.23, is significantly higher than for the dimer control,f c = 0.13, and slightly higher than for EGF-bound EGFR, = 0.19."
sparser
"The EGF binds to high and low affinity sites in EGFR-expressing cells."
reach
"EGF specifically binds to EGFR, promoting formation of either EGFR-EGFR homodimers or EGFR-ErbB-2 heterodimers and allowing the intracellular tyrosine kinase domains to approximate and undergo transautophosphorylation [XREF_BIBR - XREF_BIBR]."
sparser
"EGF binds to extracellular domain of EGFR, whereas gefitinib, a well-known EGFR inhibitor xref , binds to Tyr kinase domain."
reach
"We propose that this dynamic quenching takes place because EGF bound probes interact with tryptophan residues in the extracellular domain of the EGF and EGFR complex."
sparser
"Next in Fig. xref we compared the MC and ODE concentration profiles of EGF bound EGFR monomer in the presence of ligand (160 nM), with a receptor number density of 125 receptors per μm 2 and a low diffusivity of 2 × 10 -15 m 2 s -1 ."
reach
"A requirement for negative cooperativity is that EGFR dimers bind one EGF."
sparser
"Binding of EGF to its receptor, the epidermal growth factor receptor (EGFR), induces dimerization and activation of EGFR ( xref )."
reach
"EGF and EGFR complex translocates to the nucleus."
reach
"Neuregulin binding to these receptors is thought to promote an extension of the molecule (and exposure of the dimerization arm) similar to that seen when EGF or TGFalpha bind to EGFR, and this has been confirmed by X-ray scattering studies of the ErbB3 extracellular region in solution [XREF_BIBR]."
reach
"The EGF like domain of the Her3 ligand heregulin-alpha (HRG) was then introduced into the Her3 binding site between domains I and III, in accordance with the pose of EGFR bound EGF."
sparser
"Binding of the epidermal growth factor (EGF) ligand to EGFR induces homo- or hetrodimerization of the receptor and activation of the kinase domain, ultimately leading to intracellular signaling events, including activation of protein kinase B (AKT), extracellular signal-regulated kinase (ERK), and p38 mitogen-activated protein kinase (MAPK)."
reach
"The three-dimensional structural prediction for EGF, ATTO655, and EGFR complex showed that the interaction between EGFR and the EGF core is retained after ATTO655 conjugation, and the ATTO655 conjugated C-terminal region with high structural plasticity does not seem to interrupt either the binding of EGF to EGFR or the structure of EGFR."
reach
"These data suggest that SP-D reduces EGF binding to EGFR through the interaction between the carbohydrate recognition domain of SP-D and N-glycans of EGFR, and downregulates EGF signaling."
sparser
"It is well known that the binding of EGF to EGFR promotes the internalization of the receptor through the endocytic pathway, and the internalized EGFR is strongly associated with an early endosome marker Early Endosome Antigen 1(EEA1) that is enriched in endosomes xref ."
reach
"Furthermore, the kinase activity can be significantly stabilized by reconstituting purified EGF bound EGFR dimers in phospholipid nanodiscs or vesicles, suggesting that the environment around the hydrophobic transmembrane and amphipathic juxtamembrane domains is important for stabilizing the tyrosine kinase activity in vitro."
sparser
"Both the CellMap and NCI-PID databases correctly show EGF binding to its canonical receptor EGFR in the shortest paths, but they also suggest that the ligand can interact directly with ITGB4 (integrin-β4), PXN (Paxillin), SMAD3, or CAV1 (caveolin-1)."
sparser
"The affinity of the FabC225:sEGFR interaction is 3 orders of magnitude higher than that for interaction of EGF with isolated domain III, and 50-fold greater than for the interaction between EGF and fu[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
reach
"Interference with the EGF and EGF-R interaction through dietary or pharmacological manipulations of the urine, or via targeting strategies employing intravesical administration of conjugated toxins or isotopes is already being employed in experimental and clinical studies."
sparser
"All of the ligands are synthesized as transmembrane proteins that are inserted into the plasma membrane and are then cleaved by cell surface proteases to release the mature growth factors characterized by a consensus EGF motif that binds to EGFR."
sparser
"Conventionally, the binding of a ligand such as EGF to EGFR promotes receptor dimerization and phosphorlyation."
reach
"The solution structure of hEGF presented in this paper is different from the bound form of hEGF present in the crystal structure of the 2:2 EGF and EGFR complex because its C-tail contains a hydrophobic core."
sparser
"Functional assays measuring EGF-stimulated thymidine incorporation in the presence of TGF-beta show that the resulting long-term stimulation of EGF receptor binding is associated with an increased sensitivity to EGF."
sparser
"Binding of EGF to the EGFR at the cell surface triggers rapid internalization of the EGF-EGFR complex and subsequent sorting at the early endosome for lysosomal degradation. xref ; xref ; xref The role of Hrs in regulating endosomal trafficking of EGFR has been well established; both overexpression and knockdown of Hrs inhibit ligand-induced EGFR degradation. xref ; xref The observed interaction and colocalization of Trak1 with Hrs thus raise the possibility that Trak1 may participate in regulation of EGF-induced endocytic trafficking of EGFR to the lysosome for degradation."
reach
"XREF_BIBR, XREF_BIBR This llama nanobody was nonimmunogenic in mice and was proven to block binding of EGF to EGFR, thereby inhibiting EGFR signaling and showing the specific tumor targeting."
sparser
"For example, we described that EGF-bound EGFR is ubiquitinated by CBL and then endocytosed."
reach
"53 The EGF and EGFR complexes initially enter into the early endosomes where the EGFR kinase is still active XREF_BIBR, XREF_BIBR and traffic to the late endosome before entering the lysosome where EGFR undergoes degradation."
reach
"We thereby conclude that there is a feed-forward signaling pathway in BLBC where EGF binds to the EGFR, which in turn leads to activation of the MAP kinase and RSK pathway resulting in phosphorylation of YB-1 at S102."
sparser
"The energy transfer observed was either the result of heterodimerization of EGFRs that have bound EGF containing Alexa 488 and TMR or the exchange of differently tagged ligands on dimerized receptors ( xref )."
sparser
"After the association of EGFR with EGF, the receptor states ultimately converge to the immobile cluster, perhaps as a consequence of phosphorylation-induced changes in the conformation and electrostat[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"At 100 ng/mL EGF the EGFR forms clusters, which are subsequently internalized, whereas outside the clusters diffusivity increases and the organization of the receptor remains unchanged."
reach
"EGF binding to EGFR activates multiple signaling pathways in PCCs, such as the Ras/Raf/MAPK and Rac/JNK/MAPK -38, and EGF then undergoes rapid endocytosis."
sparser
"EGF induces dimerization of EGFR by binding to the extracellular region of the receptor to form a 2:2 EGF:EGFR complex [ xref , xref ]."
sparser
"Its ligand, epidermal growth factor (EGF), binds EGFR which then dimerizes and initiates signal transduction pathways that affect cellular proliferation, motility and invasion, apoptosis, and angiogenesis."
sparser
"In the study, we report AGN-PC-0MXVWT to be an efficient and high efficacy inhibitor of EGFR-EGF interactions identified through computational approaches."
reach
"Recent studies have shown that an EGF like repeat from the matricellular protein, tenascin, binds to and redistributes EGFR to lamellipodia."
biogrid
No evidence text available
reach
"These results indicate that extracellular MIF directly binds to the extracellular domain of EGFR and blocks the binding of EGF to EGFR, thereby inhibiting EGF induced EGFR activation."
reach
"Co-immunoprecipitation studies indicated that CDRI-85/287 like AG1478 significantly reduced the formation of EGF and EGFR complex both per se (p < 0.01) and in presence of EGF (p < 0.01) (XREF_FIG)."
sparser
"It was also worth to note that NAC pretreatment could also reverse oridonin induced EGF-EGFR binding probability decrease in KYSE-150 cells, which further proved that ROS played the key role in the effects of oridonin against EGF-EGFR interactions."
reach
"Internalization of the EGF and EGFR complex in flow cytometry."
reach
"Radio iodinated EGF bound to EGFR was displaced with either low concentrations of cetuximab or high concentrations of panitumumab."
sparser
"Yanagida and colleagues performed smFRET to study the interactions between EGF and EGF receptor (EGFR) in living cells using TIRF microscopy ( xref ). xref Since EGF binding to EGFR leads to receptor dimerization, a mixture of Cy3-labled EGF and Cy5-labled EGF were added to EGFR expressing cells."
reach
"In addition, the binding of soluble EGF to EGFR under physiological conditions is accompanied by the internalization of the EGFR and EGF complex."
sparser
"Binding of EGF to the extracellular domain of the EGF receptor induces receptor dimerization and autophosphorylation of intracellular domains."
reach
"The binding of EGF to EGFR activates downstream signals that stimulate cancer cell invasion and metastasis."
sparser
"However, based on our results, which show an association of EGF and EGF-R with infant size, and of EGF-R with infant growth rate, we suggest that EGF-R in milk may be acting in a similar manner to other soluble receptors that have been identified in milk."
bel
"Pulse-chase experiments indicated that EGF stimulated the degradation of the wild type EGF receptor but had no effect on the rate of degradation of the EGFR/RET receptor."
reach
"EGF binding to the EGF receptor stimulates the activity of intracellular kinase cascades, producing signals that consequently alter gene regulation, which leads to cell proliferation and anti-apoptogenic survival [XREF_BIBR, XREF_BIBR]."
reach
"Based on the> 20-fold reduced potencies of lapatinib for the L858R and Delta746-750 tEGFRs compared to EGF bound WT tEGFR, we hypothesize now that the kinase active conformation of EGF bound WT EGFR may be nearly isoenergetic with the kinase inactive state of EGF bound WT EGFR and largely present in the donor kinase."
reach
"For example, the egfr_481_500 sequence belongs to extracellular domain III, where anti-EGFR antibodies, such as cetuximab, nimotuzumab, and matuzumab, are known to bind and block the binding of EGF to EGFR XREF_BIBR - XREF_BIBR."
reach
"For instance, since the auto-phosphorylation reaction (v3) of the EGF and EGFR complex is rate limiting for the pathway between EGF to (EGF-EGFR) 2 *, its rate is used for the single reaction that transforms EGF to (EGF-EGFR) 2 * in the smaller model."
sparser
"Efficiently blocking the interaction between EGF and EGFR could inhibit cell proliferation and tumor growth."
sparser
"This study demonstrates that the extended configuration of EGFR does not account for the apparent high-affinity EGF-binding to EGFR on intact cells."
reach
"It has been previously reported that the binding of EGF to EGFR dimers is more than ten times stronger than to EGFR monomers."
sparser
"However, we did not find any significant associations between polymorphisms of the EGF and EGFR genes and IPSS."
sparser
"These downstream pathways such as the mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) pathway [ xref ], the phosphoinositide 3-kinase (PI3K) pathway [ xref ], and the phospholipase C (PLC) pathway [ xref ], are activated when epidermal growth factor (EGF) binds to the extracellular domain of EGFR."
reach
"In primary hepatocyte cultures, EGF or TGFalpha binding to EGF receptor activates Erk1/2 and PI3K pathways, induces cyclin D1 and thus initiates DNA synthesis."
sparser
"For the subsequent detection of bead-bound EGFR, an anti-EGFR antibody was chosen that did not interfere with the EGF-EGFR interaction, according to the data sheet."
sparser
"The EGF receptor, or fragment of the receptor, which may be associated with EGF within the vesicles may elicit the biological signal of EGF."
sparser
"In RTCs, binding of EGF to EGFR stimulates activities of ERK, phospholipase C (PLC), and SphK, resulting in increased intracellular levels of S1P, while the EGF/EGFR-mediated Ca 2+ mobilization, which may regulate apoptosis, requires activation of PLC and SphK in HEK293 cells [ xref ]."
sparser
"We observed an average of 3–5 frames per EGFEGFR binding event for all time-lapse interval measurements up through intervals of 25 s."
sparser
"Association of a second EGF molecule to a preformed EGF-EGFR complex was seen as a sudden doubling of the fluorescence intensity."
sparser
"The binding of EGF to the extracellular domain of EGFR promotes the activation of EGFR through autophosphorylation of their intracellular domains, which subsequently triggers the PI3K/Akt signaling pathway [ xref ]."
reach
"AFM-SMFS results demonstrated that oridonin could inhibit the binding between EGF and EGFR in KYSE-150 cells by decreasing the unbinding force and binding probability for EGF-EGFR complexes, which was further proved to be closely associated with the intracellular ROS level."
reach
"XREF_BIBR, XREF_BIBR In a normal tissue, the ligand EGF binds to the EGF receptor (EGFR), inducing a dimerization of one or several members of the EGF receptor family (ErbB 1-4), activating several tyrosine kinases and other downstream signal molecules promoting transcription in the cell nucleus."
sparser
"Available data indicate that retinoid-mediated mam- S.H. Flicker et al. / Cancer Letters 115 11997) 63 72 71 mary carcinoma cell growth arrest, differentiation and apoptosis is associated with d[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"The EGF binds to epidermal growth factor receptor (EGFR) to induce the expression of VEGF-A by triggering downstream Ras-MAPK, PI3K-Akt, and STAT signaling pathways, which play important roles in tumor growth and dissemination [ xref – xref ]."
reach
"The EGF family ligands exhibit a complex pattern of interactions with the four ErbB family receptors; for example, EGFR can bind eight different EGF family members (XREF_FIG) and Neuregulin 2beta (NRG2beta) binds EGFR, ErbB3, and ErbB4 (XREF_FIG)."
reach
"Levkowitz et al. reported that binding of EGF to EGFR causes EGFR degradation through binding with c-Cbl at the pY1045-EGFR XREF_BIBR."
reach
"Meanwhile, the distances from the energy minimum to the transition states along the separation paths of the EGF and EGFR complexes and TGF-alpha and EGFR complexes were deduced."
reach
"EGF binding to EGFR triggers the activation of the receptor 's intrinsic kinase activity, in turn eliciting the recruitment of many secondary signaling proteins and activation of downstream signals, such as the activation of phosphatidylinositol-3-kinase (PI3K) and Akt, a process requiring the phosphorylation of Gab1."
sparser
"The binding of EGF to EGFR activates the kinase and results in internalization of the receptor into the endosomal compartment [ xref ]."
reach
"Thus, despite the secretion of EGF into the intestinal lumen, the growth and maturational effects of EGF probably result from a specific interaction between EGF and EGFR solely at the laterobasal surface of the enterocyte."
reach
"A few years later, EGF binding to the EGF receptor was shown to transactivate PAFR, leading to cPLA2 activation and PAF production in ovarian cancer cells XREF_BIBR."
reach
"JAKi treatment of NSCLC cells led to an increase in the surface staining of EGF bound EGFR compared to control (XREF_FIG)."
reach
"TGF-alpha, amphiregulin, and EGF specifically bind EGFR."
sparser
"The EGFR inhibitors have a weaker effect in the presence of EGF that binds EGFR."
sparser
"Therefore we determined EGF expression for each of the four systems, and then treated with an inhibitor in order to block interactions between the macrophage-derived EGF and the EGF receptor (EGFR) known to be upregulated on carcinoma cells, including MDA-MB 231 [ xref , xref ]."
sparser
"In contrast, EGF binds very tightly to EGFR and is not released in the acidic environment of the endosome."
sparser
"Dimerization of EGF-bound sEGFR wild-type is essentially complete in our ITC experiments ([EGF/sEGFR] reaches 10 μM) and is negligible in FA ([EGF/sEGFR] ≤ 60 nM), yet K D for EGF binding remains the same."
reach
"EGFR binds EGF and TGF-alpha with high affinity, and both ligands are overexpressed in pancreatic cancer [XREF_BIBR]."
sparser
"For example, lower concentrations of EGF sufficient for induction of cell proliferation were associated with internalization of EGFR but not its degradation while higher concentrations of EGF promoted EGFR degradation [ xref ]."
sparser
"After the interaction of EGF with the EGFR, the receptors undergo homo- or heterodimerization that causes auto phosphorylation of certain tyrosine residues in the cytoplasmic end."
trips
"Results with (125)I-EGF indicated that SP-A interferes with EGF binding to EGFR, and a ligand blot analysis suggested that SP-A binds EGFR in A549 cells."
sparser
"It was assumed that upon binding to EGF, EGFR forms homodimers or heterodimers which result in the activation of their intrinsic kinases and autophosphorylation of specific tyrosine residues within their cytoplasmic domains [ xref ]."
sparser
"The EGF concentration is 8-fold higher than the measured IC-50 value of 1.9 nM for EGF binding to EGFR in a competition assay ( xref )."
reach
"Binding of EGF to EGFR or over-expression of receptors on the cell surface induces conformational change from closed to open conformation [XREF_BIBR, XREF_BIBR]."
sparser
"Once internalized, EGF receptor still bound to EGF gets degraded while binding to TGF α and subsequent dissociation results in recycling of EGFR [ xref ]."
sparser
"For example, EGF binds to the EGF receptor, but not to ErbB2, ErbB3, or ErbB4, and neuregulin-1 binds to ErbB3 or ErbB4, but not to the EGF receptor [ xref *, xref *]."
reach
"On the other hand, Nb VEGFR1 binds to a unique epitope and has no effect on the EGF binding to EGFR."
sparser
"It has been reported that host EGF binds to EGFR on Trypanosoma cruzi amastigotes to induce signal transduction events and cell proliferation ( xref )."
sparser
"In contrast to other RTKs, which are activated in the presence of a bivalent ligand that induces dimerization by interacting with two receptors (in a 1:2 ratio of ligand to receptor), one EGF molecule interacts with a single EGF receptor; thus, an activated receptor is constituted by a 2:2 ratio of ligand to receptor ( xref ; xref )."
reach
"This chapter provides a detailed protocol to purify and characterize full-length EGFR bound with EGF."
reach
"Upon stimulation of cells with physiological doses of EGF, EGF binds to EGFRs, whereupon the EGF and EGFR complex is internalized via clathrin mediated endocytosis, then trafficked via early and late endosomes to the lysosomal compartment, where it is degraded [XREF_BIBR]."
reach
"EGF binding to EGFR has been proposed to induce a conformational change exposing the dimerization arm in the receptor extracellular domain."
sparser
"In both male and female infants, EGF and EGF-R were positively associated with mass after controlling for infant age (Model adj."
reach
"EGF stimulates proliferation of MSCs by binding to EGF receptor (EGFR) [XREF_BIBR]."
sparser
"Furthermore, the pH sensitivity of the EGF-EGFR interaction influences the fraction of internalized receptor that is recycled back to the cell surface for continued signaling [ xref , xref ]."
reach
"The mouse monoclonal antibody (MAb) 225 was identified in 1983 as a potent inhibitor of EGF binding to EGFR, resulting in a significant decrease in proliferation of A431 epidermoid carcinoma cells displaying EGFR amplification [XREF_BIBR]."
sparser
"One difference in how these studies were conducted is temperature: those which reported AP-2 siRNA to have an effect on EGFR entry were performed at 37° C; ( xref ), while in the two which claimed no role for AP-2 in EGFR endocytosis EGF binding was performed at 4° C ( xref ; xref )."
reach
"It is now well accepted that binding of EGF (or other agonists) to EGFR shifts a monomer < => dimer equilibrium to favor the dimeric state [XREF_BIBR]."
sparser
"EGF binding to EGFR activates its intrinsic tyrosine kinase, leading to receptor autophosphorylation which triggers signaling cascades, such as MAPK and PI3K-Akt pathways."
sparser
"The present observations are supportive of the concept of paracrine or juxtacrine interactions between EGF-related growth factor ligands of luminal epithelial origin and blastocyst EGF-R in the process of implantation."
reach
"XREF_BIBR, XREF_BIBR We discovered that the binding of epidermal growth factor (EGF) to the WT EGFR extracellular domain promotes formation of a distinct antiparallel coiled coil 15 within the intracellular JM, whereas the binding of transforming growth factor-alpha (TGF-alpha) is communicated through the formation of a coiled coil - a rotational isomer - whose helical interface is ' inside-out ' compared with the JM interface formed in the presence of EGF (XREF_FIG)."
sparser
"This peptide displayed micromolar affinity towards EGF, blocked the interaction between EGF and EGFR with an IC 50 value of 60.6 μM using a cell-based EGF-EGFR inhibition assay."
reach
"The EGF and EGFR complexes initially enter into the early endosomes where the EGFR kinase is still active, and traffic to the late endosome prior to entering the lysosome where EGFR undergoes degradation."
sparser
"However, further biochemical analyses are needed to confirm that this putative EGF ligand actually binds the EGFRs present in the ctenophore and the sponge, and thus the existence of a truly functional EGFR signaling pathway in these early branching animal lineages."
sparser
"The key role of the EGFR in negative prognosis in various malignancies resulted in the development of numerous anti-EGFR agents, and several of them have been advanced to the clinic. xref Moreover, several synthetic small molecules and monoclonal antibodies targeting the EGFR family tyrosine kinases in breast cancer are in different phases of trials. xref Cetuximab is an immunoglobulin G1 chimeric mouse–human monoclonal antibody, which targets the extracellular domain of the EGFR and has been approved for combinational therapy with irinotecan in the treatment of EGFR-expressing metastatic colorectal cancer (CRC) refractory patients. xref Similarly, Panitumumab, a humanized immunoglobulin G2 monoclonal antibody, acts by blocking the interaction between the EGF and EGFR and has been approved for the treatment of EGFR-expressing metastatic CRC patients. xref Moreover, several heterocyclic small molecules have been approved for the treatment of various cancers including breast cancer."
sparser
"We used ensemble-averaged FLIM-based FRET measurement because the concentration of DiI needed to ensure colocalization of the membrane probe with EGFR-bound EGF precluded single-molecule measurement."
reach
"EGF binds to its receptor EGFR on the outer surface of the cell membrane, resulting in its activation by means of autophosphorylation and dimerization [XREF_BIBR]."
bel
"The ErbB family of four receptor tyrosine kinases occupies a central role in a wide variety of biological processes from neuronal development to breast cancer. New information continues to expand their biologic significance and to unravel the molecular mechanisms that underlie the signaling capacity of these receptors. Here, we review several aspects of ErbB receptor physiology for which new and significant information is available."
sparser
"However, the relative contribution by domains I and III to ligand binding differs between receptors, with domain III being the main contributor for EGF binding to EGFR [ xref ] whereas domain I is the dominant site of interaction for NRG binding to HER3 [ xref , xref ] and HER4 [ xref ]."
sparser
"The dimer levels were found to be associated with the apparent affinity of the EGF-EGFR interaction, with EGF binding stronger to EGFR dimers compared to monomers."
reach
"So far, it is known that all the anti-EGFR monoclonal antibodies with therapeutic activity share the property of blocking the binding of EGF to EGFR, subsequently inhibiting EGFR related downstream pathways [XREF_BIBR, XREF_BIBR, XREF_BIBR]."
sparser
"The crystal structure of the extracellular domain of EGFR bound to its ligand EGF reveals the specific key residues that are important in binding ( xref )."
sparser
"In summary, EGFR ligand concentrations in tumors predicted in the present study are within the range of K D values measured for high-affinity EGF binding to EGFR (20–200 pM) ( xref ; xref ; xref )."
sparser
"To test model predictions, we measured EGF-EGFR binding and internalization in cells expressing different levels of green fluorescent protein-EGFR."
reach
"Epidermal growth factor (EGF) binds to the EGF receptor on external cell membrane and stimulates cell signalling, modulating the activity of enzymes involved in specific pathways including transcriptional regulators, small GTPases and kinases by stimulating phosphorylation events XREF_BIBR - XREF_BIBR."
sparser
"In fact, binding of EGF to EGFR markedly enhanced entry of HCV pseudoparticles into several different cell lines and primary human hepatocytes ( xref )."
sparser
"This result is consistent with the previous SPR results that the anti-EGFR Goldbody can inhibit the binding of EGF to EGFR ( xref )."
sparser
"The interaction of EGF or any other specific ligand (e.g., TNF α , HB-EGF, and betacellulin) with EGFR (ErbB1) induces receptor dimerization, which activates an intrinsic tyrosine-specific kinase [ xref ]."
sparser
"However, this level of phosphatase activity rapidly suppresses phosphorylation of ligand-bound EGF-EGFR ( xref , upper graph)."
sparser
"Measured K D values were in the range of those reported previously for EGF-EGFR binding on other cell types xref , xref ."
sparser
"EGF binding to the extracellular region of EGFR induces receptor dimerization, which is supposed to bring the two cytoplasmic tyrosine kinase domains of the receptors close enough for autophosphorylat[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
reach
"Overall, the model shows how the efficiency of chimeric ligands is achieved : the formation of the IFN complex is enhanced by the presence of EGFR, which binds to the EGF subunit of the chimera while maintaining the IFN subunit close to the cell surface."
reach
"The interaction between EGF and EGFR promotes the dimerization and phosphorylation of receptors, thus activating a downstream signaling cascade involving MAPK, AKT and JAK-STAT."
sparser
"These reaction classes capture several aspects of EGF-EGFR and EGFR-EGFR interactions."
sparser
"Thus, tethering EGF on peptide gels likely permits sustained EGF signaling since EGFR binding to tethered EGF inhibits endocytosis and degradation."
sparser
"Interestingly, although MNNG did not affect the binding of EGF to EGFR, MNNG can interfere with EGF function."
reach
"Studies have reported that heparin binding EGF (HB-EGF) can bind to EGFR when mediated by sulfated HSPG, thereby promoting sustained phosphorylation of EGFR on Tyr1068 and Tyr992 residues and activating the downstream AKT and ERK signaling pathways to promote cancer cell proliferation and migration [XREF_BIBR]."
reach
"The binding of EGF to EGFR induces receptor activation and downstream signaling cascades important to cell proliferation, differentiation and migration XREF_BIBR - XREF_BIBR XREF_BIBR."
sparser
"With the addition of EGF, which binds EGFR and activates the Raf/MEK/ERK signaling pathway, the inhibition of phosphorylation by Raf265 was still significant."
reach
"It is well known that EGF binding to EGFR results in activation of PLCgamma1."
sparser
"In addition, cellular reactions against EGF signaling, including calcium response, start much faster than the binding of EGF to EGFR reaches the equilibrium state."
sparser
"EGF binds to the EGF receptor to initiate signal transduction through multiple downstream signal mediators."
reach
"TGFA forms a protein and protein complex with EGFR in a manner similar to EGFR and EGF complex [XREF_BIBR]."
sparser
"Instead, binding of EGF to the EGFR was confined to other membrane populations, and correlation of ligand interaction with the laterobasal membranes (LBM) was nearly perfect (p less than 0.001) across a special equilibrium gradient enriched in brush border and LBM but devoid of intracellular membranes."
sparser
"The vaccine is intended to induce antibodies against self EGFs that block EGF-EGFR interaction."
reach
"Binding of EGF to EGFR stimulates the dimerization of EGFR and the activation of its kinase, which leads to the trans-autophosphorylation of EGFR [XREF_BIBR]."
sparser
"In contrast, upon bFGF stimulation, the increased EGFR expression which was observed in our experiments, may be induced by a cross-talk between EGFR and FGFR signaling pathways which often overlap. xref FGFR activation upon bFGF binding may stimulate similar signaling transducers as binding of EGF to EGFR."
trips
"A binding study using (125)I-EGF demonstrated that SP-D downregulated the binding of EGF to EGFR."
sparser
"We conjugated EGF to Alexa 488 (donor) and Erythrosin (acceptor) (EGF and EGF) and exposed A431 cells to saturating concentrations of mAb 2E9 to block EGF binding to the LA population followed by a 1[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"The results demonstrated that EGF bound to EGFR more stably in the cells co-expressing EGFR and HER2, and the binding enhancement in the presence of HER2 was inhibited by either Trastuzumab or Pertuzumab."
reach
"Results with 125 I-EGF indicated that SP-A interferes with EGF binding to EGFR, and a ligand blot analysis suggested that SP-A binds EGFR in A549 cells."
reach
"Similarly, Lidke and colleagues demonstrated that Qdot conjugated EGF binds and activates the EGF receptor erbB1, being rapidly internalized into endosomes that exhibit active trafficking and extensive fusion in A431 cells (epidermal carcinoma) and CHO cells (chinese hamster ovary) 46."
sparser
"The inhibition BY PMA of EGF-promoted Na+/H+ exchange is not due to inhibition of EGF-binding to the EGF receptor."
sparser
"We examined the possibility that TNFα can modulate HB-EGF function and that of other EGF family ligands that bind EGFR (EGF, TGFα, and BTC) in endometrial stromal cells ( xref )."
sparser
"The EGF peptide binds to the EGF receptor, a biomarker overexpressed in 30–50% of high-grade gliomas."
sparser
"However, in aged cells Shc was unable to form stable complexes with EGFR after EGF stimulation."
sparser
"rAR84 competed poorly for binding of radiolabeled EGF to the EGF receptor and had little ability to stimulate growth of Balb/c/3T3 cells."
reach
"The binding of EGF receptor to EGF causes receptor dimerization and induces phosphorylation of tyrosine residues in tyrosine kinase domain located in the intracellular tail of receptor, which recruits many signaling proteins, such as PI3-K, PLC-gamma, PLD, Shc, and Grb2 proteins, phosphotyrosine phosphatase 1, and others, and transduces hormonal signal to different intracellular effector proteins and systems [XREF_BIBR]."
sparser
"One of the goals of this paper was to establish the biological processes behind the effect of the tyrosine kinase inhibitor gefitinib on the EGFEGFR interaction."
reach
"These data suggest that inhibition of p38 pathway delays EGF and EGFR complex internalization and degradation, decreases EGF consumption, and attenuates EGFR turnover."
reach
"Previous studies have shown that binding of EGF to the EGFR at the plasma membrane causes rapid internalization of the EGF and EGFR complex and subsequent sorting at the early endosome for delivery to the lysosome for degradation [XREF_BIBR - XREF_BIBR]."
sparser
"As shown in Figure 3 , high-affinity EGF binding by sEGFR is substantially compromised at pH values below 7.5, and cannot be detected (at this concentration) in BIAcore stud[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"Limited uptake occurred for control cells without EGF, and uptake was blocked by siRNA knockdown of EGFR in cancer cells, revealing the importance of EGF-EGFR binding."
sparser
"In contrast, none of the neuregulins bind to EGFR which instead binds unrelated ligands such as EGF, amphiregulin and others (see Fig. xref )."
sparser
"Binding of epidermal growth factor (EGF) to the EGFR activates the tyrosine kinase activity of the cytoplasmic domain of the receptor."
reach
"Subdomains of the extracellular ligand binding region of the chicken EGF receptor, which binds EGF with low affinity, were replaced by corresponding regions of the human EGF receptor, which binds EGF with high affinity."
reach
"For example, the EGF and EGFR complex undergoes the degradation pathway, whereas the TGF, alpha, and receptor complex follows the recycling pathway [XREF_BIBR]."
reach
"Furthermore, when EGF-R binds to EGF, it can move out of the caveolae with the modulation of Src kinase (Mineo et al, 1999)."
reach
"EGF was found to facilitate DNA synthesis, regeneration, tumour growth and progression of HCC cells, and bind with EGFR as the potential connection between inflammation and HCC and one of therapeutic opportunities XREF_BIBR, XREF_BIBR."
reach
"Binding of EGF to the EGF receptor (EGFR) at the cell surface induces receptor dimerization and tyrosine phosphorylation of its carboxyl-terminal tail (reviewed in 1)."
sparser
"The EGF binding to EGFR is relatively stable at the pH of endosomes, so upon EGF binding EGFR is continuously ubiquitinated by the Cbl ubiquitin ligases ( xref ) and transported to lysosomes ( xref , xref )."
sparser
"As a mitogen, hEGF stimulates cell proliferation and differentiation by binding to the extracellular domain of the EGF receptor (EGFR) dimer to form a 2:2 EGFEGFR complex."
reach
"Although the structure of EGF is very similar to that of TGF-alpha, the residues that are surface exposed when EGF is bound to EGFR are not conserved in TGF-alpha."
sparser
"Although biophysical studies suggest that monomeric EGF binds to its receptor which then forms a dimer with another monomeric EGF:EGFR complex , there is still controversy as to how the ligand–recepto[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"By contrast, the binding of EGF to the 2C-EGF receptor was markedly altered."
sparser
"Binding of EGF to the EGFR stimulates its tyrosine kinase activity via an allosteric mechanism involving the formation of asymmetric intracellular kinase domains."
reach
"As reported, EGF binding to EGFR leads to the phosphorylation of specific cytoplasmic tyrosine residues of EGFR, including positions 992, 1068, 1086, 1148 or 1173 [XREF_BIBR]."
sparser
"Previously we have shown that the anti-TK activity of the parent aptamer sequence was not due to impairment of EGF ligand–receptor binding or interference with EGFR receptor dimerization [8] ."
sparser
"For the more crucial 41 position, only the wild-type EGF was able to bind the EGFR."
reach
"These pathways are activated through two ligands alongside the two axes : 1- through the EGF binding to EGFR, and 2- via tamoxifen binding to GPR30."
sparser
"The interaction between EGF and EGFR promotes the dimerization and phosphorylation of receptors, thus activating a downstream signaling cascade involving MAPK and JAK-STAT signaling pathway ( Al Moust[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"Both proteins, however, bind the EGF receptor in an EGF-dependent manner."
sparser
"In an initial report on Y845 phosphorylation of EGFR by Src, it was noted that the phosphorylation requires not only the catalytic activity of Src but also the binding of EGF to EGFR [ xref ]."
reach
"Since both cetuximab and panitumumab have negatively charged surfaces and cetuximab lacks a central cavity it is harder to predict which single point mutations could allow binding of cetuximab but prevent panitumumab binding while maintaining a functional EGFR that can bind to EGF."
sparser
"We also show that activation of EGFR signaling, either through EGF:EGFR binding or mutations in the EGFR kinase domain, enhance Fn14 protein expression, and that pharmacological inhibition of EGFR sig[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"We hypothesized that hypoxia results in epidermal growth factor (EGF) production and that EGF binds to EGFR to activate the signaling cascade leading to arginase II induction and proliferation in hPMVEC."
reach
"To analyze whether the EGFR is involved in the migration and invasion of BGC823 and SGC7901 cells, these cells were treated with EGF, which binds to the EGFR and activates the downstream signaling pathways."
sparser
"Upon EGF binding to EGFR, dynamin GTPase is activated, which promotes the receptor's internalization xref ."
reach
"The binding of EGF to EGFR can induce nuclear translocation of EGFR [XREF_BIBR]."
reach
"Scatchard analysis of the binding of EGFR and IR with EGF and insulin, respectively, yields concave-up or curvilinear plots that indicate the presence of two classes of binding sites."
reach
"EGF and EGFR complex activates a number of biological functions through downstream PI3K and AKT, Ras/Raf/MAPK, and JAK and STAT."
reach
"High-affinity binding of epidermal growth factor (EGF) to EGF receptor is disrupted by overexpression of mutant dynamin (K44A)."
reach
"The binding affinity between EGF and EGFR was estimated by flowing the ligand (EGF) over the EGFR immobilized Sensor CM5 chip."
reach
"The naturally secreted and O GlcNAcylated MIF binds to EGFR, thereby inhibiting the binding of EGF to EGFR and EGF induced EGFR activation, phosphorylation of ERK and c-Jun, cell invasion, proliferation and brain tumour formation."
reach
"EGFR monomers on the cell surface bind a single molecule of EGF."
reach
"EGF binding to EGFR induces formation of receptor homo- and heterodimers and initiates multiple cellular signal cascades such as the Ras-Raf-MAPK pathway, which affect cellular growth."
bel
"HB-EGF and TGFA, although immunologically distinct from EGF, reportedly inhibit the binding of EGF to its own receptor"
reach
"Confocal microscopy was performed to confirm the nuclear localization of the EGF and EGFR complex in SKHep-1 cells."
sparser
"Binding of EGF by EGFR activates a sequence of receptor tyrosine kinases and regulates nuclear transcription."
sparser
"EGF binding to the EGF receptor stimulates the activity of intracellular kinase cascades, producing signals that consequently alter gene regulation, which leads to cell proliferation and anti-apoptogenic survival [ xref , xref ]."
hprd
No evidence text available
reach
"EGFR signaling is maintained by the endocytic pathway, which is crucial for the spatial localization of the EGF and EGFR complex needed to activate downstream effectors [XREF_BIBR]."
sparser
"In HT29, a human colon carcinoma cell line, flow cytometry, immunoprecipitation, and Western blot analysis, confocal spectral microscopy have demonstrated that FR18 is active at concentrations as low as 10 nM, inhibits EGF binding to EGFR while leaving unperturbed the receptor kinase activity."
sparser
"Dimers composed of one ligand-bound erbB1 and one unoccupied receptor were also relatively unstable, with a 2-fold larger off rate than dimers composed of two EGF-bound erbB1."
reach
"The binding of EGF to EGFR stimulates internalization and trafficking of the receptor and ligand complex, primarily to the lysosomes for degradation XREF_BIBR."
reach
"EGF stimulation also triggers the internalization of the EGF and EGFR complex via the canonical clathrin mediated endocytic pathway, through early and late endosomes for degradation in lysosomes."
sparser
"At the same time, receptor binding stimulates endocytosis of EGF-EGFR, resulting in the formation of cytosolic vesicles and tubules containing EGF-EGFR within their lumen."
reach
"Similarly, Lidke and colleagues demonstrated that Qdot conjugated EGF binds and activates the EGF receptor erbB1, being rapidly internalized into endosomes that exhibit active trafficking and extensive fusion in A431 cells (epidermal carcinoma) and CHO cells (chinese hamster ovary) 46."
reach
"The binding of EGF to the EGFR induces conformational changes within the receptor that increases the catalytic activity of its intrinsic tyrosine kinase and also promotes the dimerisation of receptors."
reach
"We demonstrated unequivocally that EGF and TGF-alpha interaction with EGFR is not an obligatory event in mediating estrogen stimulated cell proliferation."
sparser
"Since monoclonal antibody specific for the EGF receptor abolished both EGF binding to its receptor and tyrosine phosphorylation of the c-erbB-2 gene product, we have concluded that activation of EGF receptor tyrosine kinase activity upon EGF binding leads to the phosphorylation of the c-erbB-2 gene product on its tyrosine residues."
sparser
"Our data show that sEGFR dimers bound EGF with 30-40-fold higher affinity [KD = (2-3) x 10(-8) M] than did sEGFR monomers."
reach
"We conclude that these AONs do not significantly interfere with EGF binding to EGFR and that simultaneous acute incubation with EGF and AONs does not directly block EGF uptake."
sparser
"As a low molecular weight (Mr  = 6045) polypeptide, EGF can bind to its receptor EGFR and then trigger its autophosphorylation in several C-terminal tyrosine residues."
reach
"We have recently synthesized a peptide called Disruptin, which comprised the SVDNPHVC segment of the epidermal growth factor receptor (EGFR) that inhibits binding of heat shock protein 90 (Hsp90) to the EGFR and EGF dependent EGFR dimerization to cause EGFR degradation."
reach
"EGF then binds to the EGF-R located on crypt epithelial cells, where it transactivates the ErbB receptor, stimulating cell proliferative responses when local inflammatory cytokine patterns are altered."
reach
"After protein production and -purification, the IC50 for EGF binding to EGFR for both nanobodies was measured in ELISA and found to be 6-7 nM for the 9G8 nanobody and 10 nM for the 38G7 nanobody (XREF_FIG)."
reach
"EGF, transforming growth factor-alpha (TGF-alpha), and amphiregulin only bind to the EGF receptor."
reach
"Use of phospho specific antibodies against pY-1068 or pY-1173 of EGFR (rapidly phosphorylated after EGF binds the EGFR) on western blots of cell lysates from the three MTLn3 lines at various times after EGF stimulation (XREF_FIG) revealed no statistically significant differences in the kinetics of EGFR auto-phosphorylation (XREF_FIG)."
sparser
"Additionally, AFM was also applied to investigate the effect of Trastuzumab, as well as Pertuzumab, on HER2-modulated EGF-EGFR interactions, which demonstrated that EGF bound to EGFR more stably in the cells co-expressing EGFR and HER2, and the binding enhancement in the presence of HER2 was inhibited by either Trastuzumab or Pertuzumab [ xref ]."
reach
"Though the active EGF bound EGFR protein has not been crystallized in its entirety, the structure of the extracellular (ecto) domain of EGFR has been solved 18."
sparser
"The EGF ligands released from the oocyte may bind to EGFR on the follicle cells, resulting in an increased expression of activins, which in turn act on the oocyte to promote oocyte maturation ( Pang a[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"EGF binds to the EGF receptor (EGFR) to activate cell growth and proliferation [ xref ]."
sparser
"Hence, EGFR bound to EGF is not de-ubiquitinated and therefore continues down the endocytic path to final degradation in the lysosome."
sparser
"The recent publications indicate the EGF-bound wild-type EGFR to trans -phosphorylate the EGFRvIII initiating aberrant signaling cascade."
sparser
"A recent study also indicated that the activation of the epidermal growth factor (EGF)-EGF receptor signaling pathway is associated with the development of CK19 (+) HCC, and this pathway may account for the poor prognosis of patients [ xref ]."
sparser
"However, topical treatment in vivo with either TPA or chrysarobin significantly increases EGF binding to EGFR in the long term [ xref ]."
sparser
"A plausible explanation for decreased EGF-induced receptor dimerisation and tyrosine phosphorylation could be that addition of AnxA2 (D1/274.5) antibody blocked AnxA2 function, resulting in the inhibition of EGF binding to EGFR and/or recruitment of other proteins which are critical for EGFR function."
reach
"It is widely accepted that EGF binding to EGFR evokes a rapid enhancement ofCa 2+ influx through the membrane ion channels, inducing several Ca 2+ -related effects on cytoskeletal functions XREF_BIBR - XREF_BIBR."
sparser
"Binding of [(125)I]EGF to the wild-type EGF receptor showed a curvilinear Scatchard plot with both high- and low-affinity forms of the receptor."
sparser
"Another factor could be the presence of related EGFR family members (i.e., erbB2, erbB3, and erbB4) that form EGF-dependent heterodimers with the EGFR [43,44] ."
sparser
"Interestingly, both C225 and 528 block EGF binding to EGF receptor ( xref ) and it is clear from structural data that C225 directly contacts the ligand binding site ( xref )."
sparser
"Here, we demonstrate that MUC1 promotes the retention of EGF-bound EGFR in Early Endosome Antigen1 (EEA1)-positive vesicles while preventing its trafficking to the lysosome."
sparser
"Through the present study it is reported that at pH 6.6 the EGFR binds to EGF with decreased free energy of binding as compared to pH 7.2."
reach
"Although the EGF bound EGFR dimer is a stable structural unit in dodecyl maltoside and is functionally active, the kinase activity is markedly less stable than in Triton X-100."
sparser
"EGF binding to EGFR leads to receptor autophosphorylation, followed by clathrin-coated pit mediated endocytosis [ xref ]."
reach
"EGF binds to EGFR at the cell surface, is internalized, and remains bound to its receptor as it traffics from SEs through LEs to lysosomes [XREF_BIBR]."
sparser
"CPI was shown to inhibit EGF binding to the EGF receptor, EGF-stimulated receptor tyrosine phosphorylation and cellular proliferation, and was also demonstrated to inhibit the growth of pancreatic tumour cells in culture and in nude mice."
reach
"These ubiquitous inhibitory effects of EGCG on a series of RTKs, combined with the fact that the inhibitory effect of EGCG on EGF and EGFR binding was found only in a subcellular system [XREF_BIBR], raised a question that EGCG might inhibit RTKs by a more general mechanism."
sparser
"To investigate the FRET efficiencies that can be found in the EGFR-EGF complexes that form in A431 cells with physiological EGF concentrations, we used 12 samples of A431 cells."
reach
"The quantitative study of EGF and EGFR complex translocation to the nucleus may help to unravel its roles in health and pathological conditions, such as cancer."
sparser
"In Reactome, the activation of RAS is represented as a chain of reactions that starts with the binding of the EGF ligand to the EGFR and ends with the nucleotide exchange of RAS catalysed by SOS1 (Figure xref )."
reach
"Our results thus far demonstrate that the binding of EGF to full-length EGFR leads to formation of an antiparallel coiled coil within the intracellular JM."
sparser
"This result indicates that the binding of EGFR and EGF in MIIP-HA−overexpressing H1299 cells was not impaired but increased, partially because of the cells' feedback regulation to maintain growth."
reach
"There are, at present, only two crystal structures for intact extracellular modules of EGFR family members bound to ligands, EGFR bound to EGF, and HER4 bound to neuregulin-1beta."
sparser
"This binding induces receptor phosphorylation similar to those observed upon the binding of EGF to EGFR in mammalian cells."
reach
"Taken together, these findings support our hypothesis that hypoxia results in enhanced secretion of EGF from hPMVEC and that hPMVEC secreted EGF binds to EGFR to activate the signaling cascade ultimately leading to arginase II induction and cell proliferation."
reach
"Binding of the epidermal growth factor (EGF) to EGFR leads to receptor dimerization, autophosphorylation, and activation of downstream signaling cascades that regulate cell proliferation, motility, and survival [XREF_BIBR]."
sparser
"EGF interacts with EGFR, leading to receptor dimerization, activation of kinase activity, and autophosphorylation of EGFR [2] ."
sparser
"The binding of EGF to EGFR could activate MAPK pathway which can regulate fundamental cellular processes such as growth, proliferation, differentiation, migration and apoptosis. xref , xref , xref ."
sparser
"Once epidermal growth factor (EGF) ligand binds to the extracellular domain of the EGFR, this induces the formation of homo- or heterodimers, which subsequently activate cytoplasmic tyrosine kinase activity [ xref , xref ]."
reach
"It has previously been reported that the interaction between EGF and EGFR activates Ras and MAPK signaling pathway."
reach
"This time frame for the internalization of EGF and EGFR complexes is in accordance with results from other groups XREF_BIBR."
sparser
"Cetuximab competitively blocks the binding of the EGF and other ligands to the EGFR thus preventing the activation of the downstream TK resulting in growth arrest and apoptosis ( xref ; xref ; xref )."
reach
"This can be explained in terms of a few EGF that did make it EGF and EGFR complex, even in the presence of Anti-Apt."
sparser
"This indicated that EGF binding to the EGFR mutants was still preserved and receptor function intact."
trips
"No evidence was found for binding of the EGF-like repeats to EGFR."
sparser
"The two to three visible peaks in each of the Interaction Maps in xref demonstrate a heterogeneous nature of the binding of EGF to EGFR and provide a detailed description of both the contribution and kinetic properties of the simultaneously occurring interactions."
reach
"Epidermal growth factor (EGF) binds EGFR, while heregulin (HRG) binds HER3 and HER4, and all three receptors dimerize with HER2 as the preferred co-receptor in HER2+ breast cancer cells, thus increasing HER2 activity [XREF_BIBR]."
sparser
"Indeed, point mutations in key residues or deletion of the dimerization loop prevents EGF-induced signaling via EGFR (Ogiso et al., 2002; Garrett et al., 2002) Dimerization of EGF[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
reach
"EGF binding to its receptor not only activates diverse signaling pathways, but formation of the EGF and EGFR complex also triggers clathrin dependent endocytosis."
sparser
"Oxidation of Met111 might therefore interfere with the interaction between EGFR and EGF."
sparser
"Binding of the EGF ligand to the extracellular domain of EGFR activates the receptor and its downstream signaling pathways, ultimately activating or modulating various cellular processes [ xref ]."
sparser
"In contrast, cells that were plated on the non-mobile EGF ligand substrates were strongly adhered to the surface through EGF-EGFR binding interactions, but did not translocate ligand-receptor complexes ( xref )."
reach
"Epidermal growth factor binds to EGFR (HER1, erB1), inducing receptor dimerisation and activation of receptor tyrosine kinase activity, thereby influencing a range of key cellular functions such as cell growth, proliferation and apoptosis."
sparser
"The concomitant presence of EGFR and its ligand, EGF, is associated with enhanced tumor aggressiveness and shorter survival time xref ."
sparser
"First, a single EGF ligand binds EGFR forming a stable intermediate 1:1 EGF-EGFR ligand-receptor structure."
reach
"As showed in Figure XREF_FIG, the binding of the fusion protein to A431 cells can be efficiently blocked by the addition of EGF, which binds exclusively to EGFR."
sparser
"When epithelial cells suffered from stress, upon supplementation with EGF they bind to EGFR, leading to autophosphorylation of RTK; the interaction between EGFR and SHC/Grb2 results in the recruitment of SOS to the plasma membrane to activate Ras."
reach
"Aptamer E07 blocks EGF binding to EGFR and inhibits EGF stimulated EGFR phosphorylation."
sparser
"As the mature form of AR is truncated at the C terminus and lacks a conserved leucine residue known to be essential for high affinity binding of EGF to the EGF receptor, wild-type AR (AR1.84), a C-te[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"Work is currently underway to develop models that can explain how these interfaces might participate in the formation of EGFR-EGF complexes."
biogrid
No evidence text available
reach
"Increased EGF binding and EGFR mRNA expression in rat aorta with chronic administration of pressor angiotensin II."
reach
"Binding of EGF to the EGF receptor produces changes in intracellular calcium levels and phospholipase activity which indirectly lead to activation of protein kinase C."
sparser
"Tumour response to gefitinib is associated with EGF- and gefitinib- but not radiation-modulated EGFR expression."
reach
"EGF binds with EGFR to induce cell growth, migration, and other biological activities [XREF_BIBR]."
sparser
"These findings indicate that NLP-associated EGFR was able to bind to its natural ligand, EGF, forms dimers and multimers, and together with the kinase data strongly suggests that the full-length EGFR is functional and correctly folded."
sparser
"We conclude that these AONs do not significantly interfere with EGF binding to EGFR and that simultaneous acute incubation with EGF and AONs does not directly block EGF uptake."
reach
"Thus, EGFR can potentially be bound and activated by 7 ligands, EGF, TGF-alpha, AREG, EPGN, HB-EGF, EREG, and BTC."
reach
"Remarkably, both agents caused EGF bound ERBB1 to separate from ERBB2 (XREF_FIG H)."
sparser
"In the present study, we have investigated endosomal sorting of EGF-bound EGFR in more detail, and in agreement with previous studies [53] , we now report that the activated EGFR is concentrated with[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
reach
"As mentioned in the preceding sections, NPs are targeted to malignant cells specifically via passive targeting via the EPR and/or active targeting, frequently based upon specific molecular recognition events such as EGF and EGFR interactions."
reach
"Introduction Cetuximab, a chimeric monoclonal antibody, is a commonly used anticancer drug that prevents binding of epidermal growth factor to epidermal growth factor receptor."
reach
"Furthermore, although in flox and flox MEFs (like in HeLa cells) UVC exposed EGFR shows greater co-localization with OA1 and fibrillar PMEL than EGF bound EGFR, in WASHOUT MEFs UVC exposed and EGF bound EGFR co-stain equally well with these markers (XREF_FIG, quantified in XREF_FIG)."
reach
"Epidermal growth factor (EGF) binds to and activates its receptor ErbB1 (also known as EGF receptor (EGFR))."
reach
"EGF binds EGFR tightly and dissociates slowly."
reach
"These experiments demonstrate that the binding of EGF to EGFR is not affected by the replacement of JM domain by a (GGS) 10 linker, over the studied range of ligand concentrations."
reach
"Binding of EGF to EGFR at the plasma membrane leads to dimerization of EGFR, stimulation of its tyrosine kinase activity and initiation of downstream signaling cascades."
reach
"CT expresses epidermal growth factor receptor (EGF-R) which binds to EGF secreted by the decidua."
sparser
"By using a functional selection strategy, Nanobodies that specifically competed for EGF binding to the EGFR were isolated from "immune" phage Nanobody repertoires."
reach
"In a paracrine loop intra-tumoral macrophages secrete EGF, which binds to the EGFR on tumor cells, promoting their invasion."
sparser
"EGF binds to EGFR with high affinity."
sparser
"These findings indicate that the EGF-EGFR interaction from a single complex can be directly observed and quantitated from cell lysate and that mMAPS can be applied to protein-protein interaction for fluorescent-conjugated molecules in cells."
reach
"Epidermal growth factor (EGF), derived from the cleavage of prepro-EGF and generated primarily in kidney in humans, can bind with EGFR as well, which promotes embryonic development and stem cell regeneration and regulates ion transport."
reach
"The fact that lactacystin inhibits degradation of EGFR bound EGF at high ligand concentration suggests that proteasomal activity is involved in efficient delivery to an environment where EGF is degraded."
reach
"It seems that EGF initiates cell proliferation by binding to EGFR and rapid proliferation of remaining urothelial cells leads to hyperplastic urothelium formation [XREF_BIBR]."
sparser
"Both EGF, which binds ErbB1, and the ErbB3/ErbB4-binding ligand NRG displayed, at the molecular level, higher ligand affinity towards ErbB2-containing heterodimers owing to a decelerated off-rate [ xref ]."
reach
"It was unclear what factor mediates the interaction between EGFR and FAK and EGF induced FAK phosphorylation."
sparser
"23 Core fucosylation is required for the binding of EGF to EGFR; the EGF-induced phosphorylation of EGFR was substantially blocked in Fut8 knockout cells, and downstream signaling such as Erk and Jnk [MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"Finally, we found that when EGF‐binding to EGFR was blocked the hypoxia‐induced increase in viable cell numbers was prevented."
sparser
"Biophysical analysis of the isolated extracellular region of human EGFR suggested a positive cooperative interaction between EGF and EGFR [ xref ]."
reach
"XREF_BIBR The EGFR signaling pathway is triggered by interaction between EGFR and epidermal growth factor (EGF) known as a tumor promoter, and the intracellular signaling produced by EGF and EGFR interaction is mainly mediated through the Ras-Raf-MEK-MAPK and PI3K-PTEN-AKT signaling pathways."
reach
"Epidermal growth factor (EGF) and transforming growth factor-alpha (TGF alpha) bind with similar high affinity to the human EGF receptor."
reach
"Receptor downregulation can be avoided by presenting EGF in tethered rather than soluble form, such that it binds to the EGFR but the EGF and EGFR complex is physically restrained from internalization and downregulation by the tether that links EGF to the culture substrate."
sparser
"Although well studied, many questions still remain unanswered about the interaction of EGF with EGFR, the resulting signaling and its involvement in cancer."
sparser
"Upon addition of EGF, EGFR formed dimers without any monomerization occurring (D EGFR = 0.07 ± 0.037 μm 2 /s, xref )."
reach
"Interestingly, the negative cooperativity that is normally observed for a second EGF binding to EGFR dimers appears to depend on the juxtamembrane segment."
sparser
"Aptamer E07 blocks EGF binding to EGFR and inhibits EGF-stimulated EGFR phosphorylation."
sparser
"Compared with 059-152-Fv, the epitope of cetuximab is located near the C-terminal part of domain III and similarly prevents hydrogen bond formation of EGF with Gln384 EGFR , H409 EGFR , and Lys465 EGFR ."
reach
"Cortactin and ACK1 localize to internalized vesicles containing EGF bound to EGFR visualized by confocal microscopy."
reach
"Our results demonstrated that anti-EGFR mAb A13 interfered with EGFR proliferation signaling by blocking EGF binding to EGFR with different epitopes from those of cetuximab, suggesting that combination therapies of mAb A13 with cetuximab may prove beneficial for anti-tumor therapy."
reach
"A single EGFR on the plasma membrane binds EGF to form a pre-signalling state after which a second EGFR binds forming a homo-dimer with the first one."
sparser
"Binding of EGF to EGFR activates PI3K, whose activity is required for production of PI3P present in endosomes."
sparser
"Targeting EGFR-EGF interactions by treatment of MCF-7 cells with anti-EGFR monoclonal antibodies prior to culture on EGF prevented appreciable upregulation of MMP-2 and MMP-9 expression and activity."
reach
"Interactive with Cdc42 and aPKC, overexpressed Par6 promotes the MAPK signaling pathway and phosphorylates ERK, even in absence of EGF and EGFR binding or ligand independent EGFR phosphorylation [XREF_BIBR]."
sparser
"A requirement for negative cooperativity is that EGFR dimers bind one EGF."
sparser
"In parallel, we found that dynamin inhibition using Dynasore did not affect either HD ( xref ), ITGA6 uptake ( xref ), or ITGB4 turnover, as measured by FRAP ( xref ), although it prevented the uptake of epidermal growth factor–bound (EGF-bound) epidermal growth factor receptor (EGFR) (Supplemental Figure S7), showing that HaCaT cells are sensitive to it."
reach
"EGFR is a transmembrane receptor that has intrinsic tyrosine kinase activity and has several specific ligands, such as the epidermal growth factor that binds to EGFR and initiates multiple signaling pathways essential for different cell functions."
sparser
"Effects of oridonin on EGF-EGFR interactions in KYSE-150 cells."
sparser
"Cetuximab also efficiently inhibits binding of sEGFR to immobilized EGF ( Figure 2 C)."
reach
"Lrig1 has been implicated in inhibiting EGF binding to EGFR."
sparser
"This transient corresponds to a change in the distance between the fluorescent moieties of EGF molecules bound to high-affinity EGFR from 8.3 to 8.7 nm ( Fig. 6 B , triangl[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
reach
"However, conflicting structural models have been proposed for the architecture and stoichiometry of EGF bound EGF receptor multimers : one model involves self association of ligand bound dimers, while another model involves association via unoccupied ligand binding sites, resulting in oligomers that can bind two EGF molecules at most."
reach
"EGF exerts the regulatory effect by binding to its receptor EGFR, which is located in the cell membrane [XREF_BIBR]."
sparser
"The repair of airway epithelium is achieved primarily through the interaction of epidermal growth factor (EGF) with its epidermal growth factor receptor (EGFR) to activate the mitogen-activated protein kinase (MAPK)/extracellular regulated kinase (ERK) signaling pathway."
reach
"Subsequently, more EGF and h-R 3-dendriplexes could be uptake with binding of h-R3 and EGF to the EGFR receptors on the HepG2 tumor cell surfaces."
sparser
"EGF binds with EGFR and stimulates receptor-based dimerization, which is a prerequisite for triggering downstream signaling pathways."
sparser
"Both TGFi and EGF bind to EGF receptor and induce receptor phosphorylation."
sparser
"Preclinical experiments indicate that concentrations of ∼0.9 nM block the interaction of EGF and EGFR to 50%, and that single agent treatment with necitumumab at doses of 4–6 mg/kg every second week, corresponding to trough serum levels of ∼40 μg/mL, showed significant antitumor activity in non‐small cell lung (NSCLC), pancreatic, colon, and squamous cervical cancer xenograft tumor models. xref , xref "
sparser
"Furthermore, introduction of additional dimerization-disrupting mutants, the receiver-impairing P699G mutant and activator-impairing M952R mutant, into the Ex20Ins background ( xref ) did not significantly reduce colony formation by Ex20Ins mutants ( xref ) but did abolish colony formation by wild-type EGFR with exogenous EGF ( xref )."
sparser
"Our results demonstrated that anti-EGFR mAb A13 interfered with EGFR proliferation signaling by blocking EGF binding to EGFR with different epitopes from those of cetuximab, suggesting that combination therapies of mAb A13 with cetuximab may prove beneficial for anti-tumor therapy."
sparser
"It has been previously reported that the binding of EGF to EGFR dimers is more than ten times stronger than to EGFR monomers."
reach
"For example, EGF binds to the EGF receptor, but not to ErbB2, ErbB3, or ErbB4, and neuregulin-1 binds to ErbB3 or ErbB4, but not to the EGF receptor [XREF_BIBR *, XREF_BIBR *]."
sparser
"Cetuximab, a chimerical monoclonal EGFR IgG1 antibody, can block the binding of EGF or other ligands to EGFR and thus inhibit ligands-induced receptor phosphorylation."
sparser
"Here, to focus on the early event of EGF-induced calcium signaling, that is from the binding of EGF and EGFR to the beginning of calcium elevation throughout cells, properties of the calcium response [MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"It has been observed in our study that the antibody anti-EGFR competitively inhibits the binding of free EGF to EGFR, but not for EGF-conjugated to HAOA-coated gold nanoparticles."
sparser
"EGF binding to EGFR results as well in the activation of the anti-apoptotic STAT3 protein xref , xref , xref ."
sparser
"The most well-studied ligand–receptor interaction for EGFR across cell types is likely EGFEGFR and observations, resulting from the study of EGFEGFR initiated interaction serves as a general model o[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"Therefore we chose to model the fourth domain DIV using the inactivated structure of EGFR bound to EGF (PDB code 1NQL) as a reference xref ."
sparser
"Roovers et al have characterized a Nb against epidermal growth factor receptor (EGFR) and showed in vitro inhibition of EGF binding to EGFR and in vivo therapeutic effect on tumor in a mouse model ( xref )."
sparser
"EGF binds to the EGFR on the surface of the cells, leading to phosphorylation and activation of ERα independently from estrogen ligands [ xref ]."
sparser
"EGF binds and activates the epidermal growth factor receptor resulting in cellular proliferation, differentiation, and survival [ xref ]."
reach
"This report describes analysis of factors which regulate the binding of EGF to EGF receptor, receptor internalization, and receptor recycling."
sparser
"Compared to the binding of EGF with EGFR in the absence of HER2, the interaction between EGF and the receptor complex EGFR/HER2 increased obviously to 74.8±5.9pN (, ), while the binding probability wa[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
reach
"EGF binding to EGFR results as well in the activation of the anti-apoptotic STAT3 protein XREF_BIBR, XREF_BIBR, XREF_BIBR."
sparser
"The reported results suggest that TGFs that compete for binding to the cellular EGF receptor and EGF may have evolved from a common progenitor."
sparser
"EGF stimulation thus induces transient Tyr-phosphorylation of endogenous Tom1L1 and interaction with Tyr-phosphorylated EGFR."
sparser
"Using fluorescence resonance energy transfer, we showed that EGF interacts with EGFR in the cytoplasm and nucleus after internalization of the ligand-receptor complex in MDA-MB-468 cells."
reach
"We conclude in this study and in the accompanying article that the active extended EGFR configuration binds EGF 5- to 20-fold more strongly than the autoinhibited monomeric receptor configuration."
reach
"Binding of EGF to the EGFR (ErbB1) results in homodimerization or heterodimerization of the EGFR with any of three EGFR related receptors ErbB2-4."
reach
"EGF stimulates cell proliferation by binding to its receptor EGFR, whereas VEGF and its cognate receptor play a central role in angiogenesis."
sparser
"The binding of EGF to its receptor, EGFR , induces receptor dimerization and reorientation of the intracellular kinase domain from an inactive conformation to an active form (auto phosphorylation), w[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
reach
"ErbB1 bound to EGF has an ' extended ' conformation, in which a loop in the domain 2 is released, and serves as a coupling site for interaction with another ErbB1 molecule bound to EGF."
reach
"The epidermal growth factor receptor (EGFR) is regarded as a prototypical cargo of the MVB and ESCRT pathway and following stimulation by epidermal growth factor (EGF) EGFR and EGF complexes are internalized, sorted into MVBs, and degraded by lysosomes or recycled back to the cell membrane."
reach
"Binding of epidermal growth factor (EGF) to EGFR triggers the dimerization of EGFR followed by autophosphorylation of its tyrosine residues, thereby activating its downstream signaling-pathway involving ERK and MAPK and PI3K-AKT-mTOR [XREF_BIBR, XREF_BIBR]."
bel
"In response to stimulation with epidermal growth factor (EGF), the guanine nucleotide exchange factor human SOS1 (hSOS1) promotes the activation of Ras by forming a complex with Grb2 and the human EGF receptor (hEGFR)"
sparser
"In numerous cell types, binding of EGF to the EGFR stimulates the tyrosine kinase activity of the receptor eventually leading to cell proliferation."
sparser
"The antibodies elicited by vaccination provoke an immune-castration of EGF, which hampers EGF-EGFR interaction."
sparser
"Recent studies also demonstrate that the ErbB3 receptor functions as a substrate for the EGFR and becomes phosphorylated when EGF binds to the EGFR [36] ."
reach
"EGF binding to EGFR increases the ERK and AKT phosphorylation level in these cancer cells."
sparser
"Epiregulin (EREG) belongs to the epidermal growth factor (EGF) family, which binds to the epidermal growth factor receptor (EGFR) to regulate the immune response of the host during infections."
reach
"Heparin binding EGF (HB-EGF), a newly discovered member of the EGF family of mitogens, binds to the EGF receptor (EGFR) and to heparan sulfate proteoglycans on the cell surface."
reach
"EGF binds with high affinity to the EGF-R and activates a variety of second messenger pathways that affect cellular proliferation."
sparser
"Epidermal growth factor (EGF), a polypeptide growth factor, binds to the EGF receptor (EGFR), a receptor tyrosine kinase."
sparser
"EGF binding to EGFR increases the ERK and AKT phosphorylation level in these cancer cells."
sparser
"EGFR binds to its cognate ligand EGF (Epidermal Growth Factor) which induces receptor tyrosine phosphorylation and promotes cross talk between EGFR family members and other signaling pathways."
reach
"In supporting of this notion, the EGF and EGFR complex was indeed detected in the nucleus using the cross linking experiment between EGF and EGFR [XREF_BIBR]."
reach
"In ovarian cancer cells, EGF binds to the EGF receptor (EGFR), thus inducing EMT activation through phosphorylation of the Janus kinase and signal transducer and activator of transcription (JAK and STAT3) pathway."
reach
"In the studies described herein we demonstrate that EGF binding to EGFR is necessary for arginase II induction and cellular proliferation."
reach
"Recent studies by Nishimura et al [XREF_BIBR] indicated a role of LIMK1 in regulating endocytic trafficking of EGFR wherein LIMK1 delays internalization of EGFR bound EGF, thereby maintaining sustained activation of EGF and EGFR axis in invasive tumor cells."
sparser
"Asbestos fibers, but not the nonfibrous analog riebeckite, abolished binding of EGF to the EGFR."
reach
"The expression of total and phosphorylated EGFR was analyzed on Western blots and EGF binding to EGFR was evaluated by fluorescence microscopy of Alexa488 labelled EGF."
sparser
"Binding of epidermal growth factor (EGF) to EGFR leads to activation of EGFR kinase and subsequent autophosphorylation and activation of downstream signaling cascades [ xref ]."
sparser
"Inhibiting EGFR with tryphostin AG1478 prevents ERK activation upon ATP addition showing that ERK activation depends on secreted EGF binding to EGFR ( xref )."
reach
"Betacellulin, heparin binding EGF and epiregulin bind to both EGFR and HER4."
reach
"Humoral variations of EGF levels are sensed by the EGFR system though the EGF and EGFR binding."
reach
"Binding of EGF to EGFR increases activation of Erk which leads to phosphorylation of alpha-catenin and transactivation of beta-catenin [XREF_BIBR]."
reach
"Apparently, ErbB2 inhibits internalization of EGF bound EGFR by constitutively driving EGFR-ErbB2 hetero and oligomerization."
sparser
"Moreover, the data suggest that AONs did not interfere with the binding of EGF to EGFR in a cell-free system."
sparser
"When cells were exposed to EGF and PS NPs, EGFR and PS NPs co-localized in the cytoplasm ( xref C), indicating that cellular uptake of PS NPs involves the binding of aggregated EGFEGFR complexes and PS NPs."
sparser
"EGFR , a cell surface protein, binds to the epidermal growth factor."
reach
"Binding of EGF to EGFR leads to the downregulation of FAK kinase activity in human carcinoma cells overexpressing EGFR."
reach
"In the context of the ligand induced dimerization model, these results suggest that the species eluting from gel filtration with apparent Stokes radii of 5.9 and 7.6 nm correspond to the inactive Delta998-EGFR monomer and EGF bound Delta998 and EGFR dimer, respectively."
sparser
"This conformational change may disrupt the C-terminal hydrophobic core of free hEGF and expose its C-terminal receptor binding site so that hEGF can bind to hEGFR and initiate signal transduction."
sparser
"The binding of EGF to EGFR shows a degree of sensitivity to pH, and disengagement of the ligand leads to recycling of internalized receptors to the plasma membrane [ xref ]."
sparser
"By contrast, mutations in the region near the hydrophobic pocket that accommodates the leucine side chains of the growth factor ligand and the tyrosine 102 of the FabC225 disrupt binding of sEGFR to b[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
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"These include : EGF, TGFalpha and amphiregulin (AREG), which bind specifically to EGFR; heparin binding EGF like growth factor, betacellulin and epiregulin which bind to both EGFR and ErbB4 [XREF_BIBR]; and the neuregulins and heregulins (HRGs), which are specific for ErbB3 and ErbB4 [XREF_BIBR]."
sparser
"So, for example, it is commonly assumed that the functional form of the hGH/hGH-R complex on cells involves a termolecular complex in which a single hGH molecule is bound to two molecules of hGH-R, and similarly that the functional complex for EGF-R is a 2:2 complex in which two EGF-bound EGF-R molecules are associated back-to-back."
sparser
"Together, EGF and EGFR are associated with brain injury paradigms and their expression is elevated in brain lesions in several brain injury conditions, but by contrast EGF levels are reduced in both rabbits and infants with IVH."
sparser
"These data suggest that the direct interaction of EGF with the EGFR ligand-binding domain modulates HSV-1 entry."
sparser
"Consistent with the ability of this method to reveal the dynamics of signaling protein interactions, we observed that cells treated with EGF induced the interaction of EGF with EGFR and the autophosphorylation of EGFR, but this interaction decreased with longer treatment time."
reach
"First, 7D12, has a reported dissociation constant for EGFR binding of 200 nM, 18 which is substantially higher than the dissociation constant for EGF binding to EGFR, 5 nM, 41 indicating weaker binding."
sparser
"In contrast, MIF, which is O -GlcNAcylated at its C-terminal Ser 112/Thr 113 on a β-strand xref , binds to EGFR directly and competitively inhibits the binding of EGF to EGFR."
sparser
"While binding of EGF to EGFR induces autophosphorylation at tyrosine sites in the C-terminal non-catalytic domain of the receptor that act as docking sites for various signaling molecules, Src transactivation of EGFR is characterized by phosphorylation at a unique Y845 site located in the kinase domain of EGFR that leads to activation of alternate signaling molecules [ xref – xref ]."
reach
"For example, EGF and EGFR complex was detected in the nucleus in MDA-MB-468 cells [XREF_BIBR]."
sparser
"One EGF molecule binds to one ErbB1 molecule, leading to the formation of a homo-dimer."
sparser
"Binding of these sites to the same type of recep- tor molecule, as seen in the hGH-hGHR and EGF-EGFR interactions, will lead to receptor homodimerization."
sparser
"The binding of EGF to an EGF receptor (EGFR) can promote cell survival and proliferation (Cohen et al ., xref ; Prenzel et al ., xref )."
reach
"Autophagy inhibition was confirmed biochemically by western blotting and immunostaining (XREF_FIG) and also by functional assays that demonstrated autophagosome accumulation, failed fusion with lysosomes and inhibition of EGF and EGFR complex degradation (XREF_FIG)."
sparser
"Single-molecule tracking reveals that the predominant mechanism of dimerization involves the formation of a cell-surface complex of one EGF molecule and an EGFR dimer, followed by the direct arrest of a second EGF molecule, indicating that the EGFR dimers were probably preformed before the binding of the second EGF molecule."
sparser
"The decreased rupture force for EGF-EGFR complex in KYSE-150 cells upon oridonin treatment demonstrated that oridonin treatment could decrease the stability of EGF-EGFR complex in KYSE-150 cells, which resulted in smaller forces to break EGF-EGFR binding."
reach
"Upon EGF stimulation, EGF bound EGFR on the cell surface is internalized by endocytosis."
reach
"Efficiently blocking the interaction between EGF and EGFR could inhibit cell proliferation and tumor growth."
sparser
"Subdomains of the extracellular ligand-binding region of the chicken EGF receptor, which binds EGF with low affinity, were replaced by corresponding regions of the human EGF receptor, which binds EGF with high affinity."
reach
"Although p38 inhibition led to prolonged surface expression of EGF and EGFR complex, the EGFR downstream signaling activity was decreased."
reach
"It has been shown that the binding of EGF to EGFR rapidly induces clustering and internalization of the ligand-receptor complexes, ultimately resulting in lysosomal degradation of both EGF and its receptor XREF_BIBR."
reach
"These results suggest that ionizing radiation may modulate the function of EGF and EGF-R interaction through the direct activation of protein kinase C."
reach
"After incubation in HEPES buffer (pH 7.0) for 1h to facilitate an efficient interaction between the EGFR and EGF, the fractions of cytoplasm and cell wall of yeast cells were prepared, and then analyzed with anti-EGFR phosphotyrosine (pY) antibodies against pY-1068, pY-1148 and pY-1173 (XREF_FIG)."
reach
"The group found that the binding of EGF to EGFR stimulates EGFR to undertake a " receiver " kinase position, resulting in the EGFR kinase domain being activated prior to the Her2 kinase domain - a novel finding which had not previously been appreciated."
sparser
"To determine the ability of monoclonal antibodies to block EGFR-NLPs binding to EGF, we again used the BLI system depicted in xref ."
reach
"The epidermal growth factor (EGF), which binds to EGFR and ErbB1, was one of the first growth factors to be described [XREF_BIBR]."
reach
"Necitumumab inhibited the interaction of EGF and EGFR with a concentration that inhibits binding by 50% of approximately 0.9 nM (0.13 mg/L) and demonstrated antitumor activity during in vivo experiments associated with trough plasma concentrations of approximately 40mg/L."
reach
"However, the transfection levels were significantly lower upon systemic application of EGF-free complexes, indicating that specific delivery is mediated by the interaction between EGF and EGFR, which corroborated the conclusion of the in vitro competition study using free mEGF."
sparser
"In human primary MFs, TNFα induces epidermal growth factor receptor (EGFR) expression and binding of EGF-EGFR induces ERK signaling activation, which results in increased Cox2 expression ( xref , xref )."
reach
"We discovered that binding of the growth factor EGF to the EGFR extracellular domain induced the formation of a discrete anti-parallel coiled coil within the juxtamembrane-A (JM-A) segment, whereas binding of the alternative growth factor TGF-alpha induced an alternative, helical interface whose structure was not established (XREF_FIG)."
sparser
"The group found that the binding of EGF to EGFR stimulates EGFR to undertake a “receiver” kinase position, resulting in the EGFR kinase domain being activated prior to the Her2 kinase domain - a novel finding which had not previously been appreciated."
sparser
"Our results demonstrated that oridonin could dramatically inhibit EGF-EGFR interactions through decreasing the unbinding force, decrease the binding probability, decreasing the energy barrier width, increasing the dissociation off rate constant and decreasing the activation energy in ROS dependent way."
sparser
"EAST was shown to bind to the EGF receptor upon EGF stimulation and to co-localize with clathrin through association with Eps15 [18] ."
sparser
"This eliminates high affinity binding of EGF to cell-surface EGFR ( xref ) and thus inhibits EGFR activation in a negative feedback loop."
sparser
"Previous studies have shown that binding of EGF to the EGFR at the plasma membrane causes rapid internalization of the EGF-EGFR complex and subsequent sorting at the early endosome for delivery to the lysosome for degradation [ xref – xref ]."
reach
"To this end, we studied physical and functional interactions between AON, EGF, and EGFR."
sparser
"Binding of EGF to EGFR increases activation of Erk which leads to phosphorylation of α-catenin and transactivation of β-catenin [ xref ]."
sparser
"Time-lapse interval measurements on the binding of EGFEGFR were performed by using the same experimental conditions as with the continuous measurements, but with 1, 2, 5, 10, 25, 40, and 100 s shuttered intervals in between frames."
reach
"XREF_TABLE summarizes the results of the most relevant clinical trials targeting the EGF and EGFR complex."
sparser
"In cells, the deletion of the leucine zipper caused a decrease in both the tyrosine phosphorylation of Cbl and its association with the epidermal growth factor receptor following stimulation with epidermal growth factor, thus demonstrating a role for the leucine zipper in c-Cbl's signaling functions."
sparser
"Heparin-binding EGF (HB-EGF) is a new member of the EGF family that binds to the EGF receptor acting as a mitogen in several systems."
sparser
"Beyond the original knowledge of increased tumor cell proliferation and pro-survival effect of EGF-EGFR interaction, a new concept of EGF-induced tumor suppression was remarkable."
trips
"These data suggest that SP-D reduces EGF binding to EGFR through the interaction between the carbohydrate recognition domain of SP-D and N-glycans of EGFR, and downregulates EGF signaling."
reach
"EGF stimulated invasiveness was inhibited by anti-EGF antibody, which is able to neutralize the binding of EGF to EGF receptor, in the invasion assay system."
reach
"EGF binds with EGFR and stimulates receptor based dimerization, which is a prerequisite for triggering downstream signaling pathways."
reach
"These results indicate the complex interactions between EGF and EGFR and their relevance in modifying tumour cell behaviour."
sparser
"Such an EGF-EGFR interaction is definitely associated with the initiation of bowel inflammation [ xref ]."
reach
"Binding of EGF to EGFR triggers rapid dimerisation and phosphorylation, followed by receptor internalisation and downregulation from the cell surface."
reach
"The binding of EGF to EGFR at the plasma membrane (PM) induces the dimerization of EGFR, which results in the activation of the EGFR tyrosine kinase and its trans-autophosphorylation."
reach
"Herein, we include the EGF and EGFR complex and associated downstream signaling culminating in matrix metalloproteinase (MMP) expression."
sparser
"Therefore, it is possible that the stable, dimeric EGFEGFR complexes determined here could be formed via some intermediate state(s), in which EGF might bind to EGFR in different mode(s)."
sparser
"When EGF binding to EGFR is enhanced, tumour aggressiveness apparently increases [ xref ]."
sparser
"For a quantitative analysis of the kinase activity of tEGFR, we used a radiometric assay to compare the effects of EGF and the Fab fragment cetuximab, an anti-EGFR antibody that blocks EGF binding and formation of extracellular EGFR dimers, on the ability of tEGFR to phosphorylate a biotinylated peptide substrate."
sparser
"A similar mechanism was also observed for mutationally inactivated kinase domain (Deb et al., xref ), where an EGF-bound mutant EGFR could still induce MAPK and PI3K pathway activation through ErbB2."
reach
"With this system, we observed oscillatory behavior of the monomer to dimer ratio of EGF bound EGFR."
reach
"The current data show that GCN4-ICM forms multimers, because the cross correlation value for this construct, f c = 0.23, is significantly higher than for the dimer control, f c = 0.13, and slightly higher than for EGF bound EGFR, = 0.19."
sparser
"EGF and EGF receptor (EGFR) interaction plays a pivotal role in cell proliferation, differentiation, and tumorigenesis of epithelial tissues."
reach
"We hypothesized that hypoxia results in epidermal growth factor (EGF) production and that EGF binds to EGFR to activate the signaling cascade leading to arginase II induction and proliferation in hPMVEC."
reach
"Binding of EGF to EGFR has been shown to induce dimerization, as well as higher order clustering of the receptor in a kinase-activity-dependent manner."
sparser
"Heparin-binding EGF-like growth factor (HB-EGF) and amphiregulin (AREG) are the members of EGF family that bind to common EGF receptor (EGFR) in the epidermis."
reach
"In other words, the binding of EGF to the EGFR extracellular domain results in a structural change leading to assembly of the intracellular asymmetric kinase interface; this structural signal is transmitted through the formation of an antiparallel coiled coil within the JM segment to which the binding and fluorescence of ReAsH is linked."
reach
"We also treated the 3D in vitro models with EGF, which binds to the ErbB1 receptor (also known as EGFR or HER1), leading to multiple pathway activations that lead, among other things, to an increased DNA synthesis and cellular proliferation XREF_BIBR."
reach
"The requirement for the WASH complex in segregation of stress internalized EGFR along with pre-melanosomal factors away from ligand stimulated EGFR contrasts with the unimpaired lysosomal degradation of EGF bound EGFR and plasma membrane recycling of TfR in WASH knockouts XREF_BIBR."
reach
"It could explain reports that ErbB2 is a preferred heterodimerization partner of EGFR, and might also explain why EGF binds more tightly to EGFR in cells where it can form heterodimers with ErbB2 than in cells lacking ErbB2, where only EGFR homodimers can form."
sparser
"The binding of EGF to EGFR can induce nuclear translocation of EGFR [ xref ]."
sparser
"The information derived from the docking analyses strongly suggests that sPLA2-IIa can directly bind to and extensively interact with the ECD of EGFR, bringing the two lobes of EGFR into an active conformation in a manner analogous with EGF binding to EGFR."
reach
"For instance, EGFR and integrins can cooperatively induce a single signaling pathway or potentiate EGFR and EGF complex formation, which can result in enhanced integrin expression."
reach
"Two notable examples are discussed here : (1) In canonical receptor tyrosine kinase (RTK) pathways, binding of the epidermal growth factor (EGF) to its receptor EGFR and ErbB1 at the cell surface triggers receptor dimerization and cross phosphorylation of the EGFR intracellular kinase domains; phosphorylated domains then recruit and activate effectors such as the small GTPase Ras and the lipid kinase PI3K."
reach
"This treatment led to the internalization of nonactivated EGF and EGFR complex into endosomes."
reach
"A previous study reported that the binding of EGF to EGFR was increased after dexamethasone treatment, which might due to dexamethasone mediated change in the micro-domain of cell surface [XREF_BIBR]."
reach
"As seen in XREF_FIG, 60 min after incubation of cells with EGF, approximately 50% of EGFR was lost as the EGFR and EGF complex trafficked to lysosomes where it was degraded."
sparser
"These AFM results provide novel information about the binding between EGF and EGFR in KYSE-150 cells at single molecule level, and also enhance our understanding about how oridonin inhibit EGFR signaling events by inhibiting EGF-EGFR binding, which is very important for the pharmacological studies of oridonin against EGFR in living cancer cells."
sparser
"In the case of EGFR, it was well established that two ligand molecules (which are monomeric when studied alone) are bound in an EGF-induced dimer of the EGFR extracellular region (Domagala et al., 20[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"The remove of excessive intracellular ROS induced by oridonin could dramatically reverse oridonin inhibited EGF-EGFR binding in KYSE-150 cells, indicating that ROS played a critical role in oridonin inhibited EGF-EGFR binding."
sparser
"EGCG treatment alters the lipid rafts of cancer cells and inhibits binding of the ligand epidermal growth factor (EGF) to the EGF receptor (EGFR), which is an RTK as well, and the subsequent receptor dimerization [ xref ]."
sparser
"Binding of EGF to the EGFR normally results in lysosomal degradation of both EGF and EGFR."
sparser
"In some cases, ligand binding can influence the fate of a receptor: for example, internalized EGF-R bound to EGF is targeted to lysosomes for degradation, whereas the alternative ligand TGF-α is released from the receptor in acidic EEs, resulting in efficient EGF-R recycling to the plasma membrane ( xref )."
sparser
"Upon ligand binding by EGF, EGFR forms dimers, either homodimers or heterodimers with another member of the ErbB family HER2 [ xref ]."
sparser
"EGF binds to the EGFR recruiting a second erbB receptor with which to dimerize ( Fig. 4 ) ."
reach
"EGF predominantly binds EGFR to induce both EGFR homodimers and EGFR-HER2 heterodimers, whereas HRG predominantly binds HER3 and HER4, inducing HER2-HER3 and HER2-HER4 heterodimers."
sparser
"The expression of total and phosphorylated EGFR was analyzed on Western blots and EGF-binding to EGFR was evaluated by fluorescence microscopy of Alexa488-labelled EGF."
sparser
"In a paracrine loop intra-tumoral macrophages secrete EGF, which binds to the EGFR on tumor cells, promoting their invasion."
sparser
"Finally, EGF binds to EGFR and activates PLC and PKC cell signaling, and therefore we examined whether PLC or PKC inhibitors could prevent MMP-2-induced EGFR activation and increased ROS formation in [MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
reach
"This study demonstrates that the extended configuration of EGFR does not account for the apparent high-affinity EGF binding to EGFR on intact cells."
sparser
"Since cytoplasmic vesicles were previously found to be EGF-positive, these results may be due to the presence of the EGF-EGFR complex that is internalized after binding of EGF to the surface EGFR."
sparser
"Once on the membrane, EGF binding to EGFR leads to receptor phosphorylation."
sparser
"Binding of EGF ligand to EGF receptor tyrosine kinase activates the receptor and downstream signaling cascades."
reach
"We hypothesized that binding of nimotuzumab to EGFR impeded the interaction between EGF and EGFR, thus inhibiting the activation of downstream cell signals and preventing increased cellular proliferation and invasive potential."
sparser
"Given that MUC1 is known to affect epidermal growth factor (EGF) interaction with its receptor EGFR in breast cancer [ xref ], we hypothesized that another growth factor may associate with MUC1."
reach