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Statements

HCN1 binds REST. 18 / 18
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"Indeed, transiently restoring HCN channel expression by disrupting the interaction between the NRSF and HCN1 delays the onset of spontaneous seizure activity following termination of SE ( xref )."
24756635
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"McClelland et al. (2012) showed that REST binding to the RE1 element in the HCN1 promoter in the hippocampus was augmented two days after kainate-induced status epilepticus, and that intraventricular administration of oligodeoxynucleotides targeted to the HCN1-RE1 both disrupted REST binding to the HCN1 promoter and prevented downregulation of HCN1 protein."
23216574
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"Blocking REST and NRSF binding to HCN1 prevented the down-regulation of HCN1 and resulted in fewer spontaneous seizures."
28279785
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"Seizure activity resulted in NRSF binding to the Hcn1 promoter in the hippocampus of kainite-treated animals ( xref ); decreased Hcn1 transcript levels as well as attenuation of I h were observed."
24418102
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"Administration of decoy ODNs comprising the NRSF DNA binding sequence (NRSE) in vitro and in vivo, reduced NRSF binding to hcn1, prevented its repression and restored I h function."
21905079
sparser
"McClelland and colleagues ( xref ) have recently shown that REST binding to the RE1 site of HCN1 is enhanced 2 days after kainic acid–induced SE."
25196047
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"Binding of REST to Hcn1 is increased after the induction of status epilepticus in a rodent model of TLE, resulting in decreased HCN1 mediated ionic currents (I h) and altered function of CA1 pyramidal cells."
23595016
sparser
"Using chromatin immunoprecipitation (ChIP) followed by quantitative PCR ( xref ) to examine if the physical binding of NRSF to the regulatory region of the hcn1 gene was augmented in hippocampi from KA rats, and if this augmented binding was selective, we detected NRSF binding at the NRSE site of the hcn1 gene ( xref ), but not at a downstream region lacking an NRSE sequence ( xref )."
21905079
sparser
"In KA-treated rats, NRSF binding to the hcn1 gene (but not the hcn2 gene xref ; Sham vs. KA p>0.05, not shown) was significantly augmented ( xref )."
21905079
sparser
"Administration of oligonucleotides targeting the Hcn1 -NRSE blocked REST binding of Hcn1 , thereby restoring HCN1 protein levels and I h current amplitudes as well as producing fewer spontaneous seizures ( xref )."
24418102
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"Therefore, we examined whether the decoy ODN treatment, blocking the interaction of NRSF with hcn1 and other target genes, influenced the outcome of KA-SE."
21905079
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"If NRSF binds to hcn1 to repress its transcription, then using an excess amount of a decoy NRSF-binding oligodeoxynucloetide (ODN) should prevent NRSF from binding the NRSE sequence on the hcn1 gene and prevent the transcriptional repression of HCN1 ( xref )."
21905079
sparser
"Administration of decoy ODNs comprising the NRSF DNA-binding sequence (NRSE) in vitro and in vivo , reduced NRSF binding to hcn1, prevented its repression and restored I h function."
21905079
sparser
"Administration of decoy ODNs comprising the NRSF DNA-binding sequence (neuron restrictive silencer element [NRSE]), in vitro and in vivo, reduced NRSF binding to Hcn1, prevented its repression, and restored I(h) function."
21905079
reach
"Administration of decoy ODNs comprising the NRSF DNA binding sequence (neuron restrictive silencer element [NRSE]), in vitro and in vivo, reduced NRSF binding to Hcn1, prevented its repression, and restored I (h) function."
21905079
reach
"If NRSF binds to hcn1 to repress its transcription, then using an excess amount of a decoy NRSF binding oligodeoxynucloetide (ODN) should prevent NRSF from binding the NRSE sequence on the hcn1 gene and prevent the transcriptional repression of HCN1 (XREF_FIG)."
21905079
sparser
"Binding of REST to Hcn1 is increased after the induction of status epilepticus in a rodent model of TLE, resulting in decreased HCN1-mediated ionic currents ( I h ) and altered function of CA1 pyramidal cells."
23595016
reach
"We ascribe the significant reduction of HCN1 channel expression to the significantly increased transcription factor, NRSF, which can bind to the HCN1 gene promoter and reduce the HCN1 channel gene transcription."
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