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CDK1 phosphorylates NPM1. 21 / 21
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"Nonetheless, the NPM Serine 125 phosphorylation is specific since the NPM fragments 1-180 and 175-222 are not phosphorylated by Cdc2."
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"Phosphorylation of Npm is catalyzed by CK2 and the cyclin dependent kinase cdc2 and cdk1."
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"NPM1 has also been reported to be phosphorylated by cdc2 kinase during mitosis (Peter et al. 1990) and by nuclear casein kinase 2 during interphase (Chan et al. 1990)."
| PMC
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"Cdk1 phosphorylates the centriolar linker protein NPM1 leading to centrosome amplification."
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"Source dataExtended Data Fig. 8: a, In vitro phosphorylation of NPM1 IDR by CDK1/cyclin B. Purified NPM1-IDR (wild-type (WT) and phosphomimetic mutant (Pm)) was incubated with CDK1/cyclin B at room temperature for 60 min."
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"Analysis of the tryptic phosphopeptide map of numatrin phosphorylated by these cdc2 kinase complexes showed that both kinase complexes phosphorylated two major identical peptides, but minor additional peptides were differentially phosphorylated by each of these kinases."
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"Feuerstein, N. (1991) Phosphorylation of numatrin and other nuclear proteins by cdc2 containing CTD kinase cdc2lp58."
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"In vitro kinase assays demonstrated that the T199 residue of NPM1 could be phosphorylated by cdk1 and cyclinB1 complex, while the phospho deficient mutant, NPM1-T199A does not serve as an efficient substrate for cdk1 (XREF_SUPPLEMENTARY)."
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"An in vitro kinase assay showed that phosphorylated-NPM at Thr199 by CDK1 enhanced NPM phosphorylation at Ser4 by PLK1 as revealed by Western blotting with the phospho-specific NPM antibodies ( xref )."
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"Our results indicated that nucleophosmin and B23, phosphorylated by prometaphase cellular extracts (nocodazole arrested cells) or cdc2 kinase, could still be co-immunoprecipitated with protein C23."
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"CDK1 phosphorylation of NPM at threonine 234/237 is critical in HCC migration and invasion."
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"We show that both cdc2 kinase complexes can phosphorylate numatrin."
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"We show that both cdc2 kinase complexes can phosphorylate numatrin."
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"In this study, we have demonstrated that T199 residue of NPM1 is a target of cdk1, and early phosphorylation of NPM1 by cdk1 leads to a premature increase in centrosome number."
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"NPM1 is recognized as a nucleolar phosphoprotein and several kinases, for instance casein kinase 2 (CKII) [ xref ], polo-like kinase 2 (Plk2) [ xref ], CDC2 [ xref ] and cyclin E/CDK2 complex [ xref ] can phosphorylate NPM1."
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"Analysis of the tryptic phosphopeptide map of numatrin phosphorylated by these cdc2 kinase complexes showed that both kinase complexes phosphorylated two major identical peptides, but minor additional peptides were differentially phosphorylated by each of these kinases."
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"In this report, we showed that phosphorylation of NPM by CDK1 including Thr199 induces sequential phosphorylation of Ser4 by PLK1."
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"We also confirmed this sequential phosphorylation of NPM by CDK1-PLK1 in MCF-7 cells ( xref )."
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"Nucleophosmin and B23 is highly phosphorylated by cdc2 kinase during mitosis, and this phosphorylation most probably has a role in initiating and controlling the entry of cells into mitosis [Peter, Nakagawa, Doree, Labbe and Nigg (1990) Cell 60, 791-801]."
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"Our data does not exclude the possibility that cdk1 phosphorylates other centrosomal proteins, in addition to NPM1, and that these substrates might also contribute to the increase in centrosome number observed upon loss of 14-3-3gamma (XREF_FIG)."
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"Nonetheless, the NPM Serine 125 phosphorylation is specific since the NPM fragments 1–180 and 175–222 are not phosphorylated by Cdc2 ( xref )."
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