IndraLab

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"To test whether Tnfaip3 -/- cells might release such ligands, we stimulated LPS primed control BMDMs with supernatants from LPS stimulated Tnfaip3 -/- cells and assayed for additional IL-1beta production by control cells."

eidos
"As shown in 420 Figure 6C , both LPS , and the 1 +2 mixture co-added with LPS , induced significant 421 expression of A20 gene within 60 minutes after addition to the cells ."
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"Secondly, in parallel to what was observed in cambinol treated cells, we also observed a significant upregulation of such LPS induced genes as ADORA2A, RUNX3, TNFAIP3 and SLC1A2 in MACs treated with DAC following LPS stimulation."

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"The LPS induced immune markers, specifically TLR4, TNF alpha induced protein 3 (TNFAIP3) and interleukin-1 receptor associated kinase like 2 (IRAK2), were identified among the strongest variables of the PC1, along with the increase in expression of genes related to mitochondrial metabolism (e.g. NADH dehydrogenase (ubiquinone) 1 beta sub-complex 5; NDUFB5)."

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"While LPS upregulated both IL-12p40 and TNFAIP3 and A20 production, adding DEHP to cultures dramatically reduced IL-12p40 and TNFAIP3 and A20 levels."

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"We thus utilized anti-G-G antibody assisted mass spectrometry to find potential ubiquitinated IL-1beta peptides from LPS stimulated Tnfaip3 -/- BMDMs."

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"These results showed that TNFAIP3 induced by LPS is insufficient for persistently inflammatory stimulation, and TNFAIP3 may be important for the metabolism in nucleus pulposus cells, providing a new research idea for the treatment and prevention of degenerative diseases."