The INDRA Database

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"Significantly, hemin inhibited HRI constructs missing the N-terminal heme-binding domain and kinase insert region, suggesting that heme inhibition of phosphorylation is primarily occurring through the heme-binding site within the kinase domain outside the kinase insertion sequence, without significant contribution from the N-terminal heme-binding domain."

36063997

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"HRI is activated by hemin deficiency and oxidative stress, PKR by viral infection, PERK by endoplasmic reticulum (ER) stress, and GCN2 by amino acid deficiency and ultraviolet (UV) irradiation."

38734221

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"We also investigated the mode of heme regulation of HRI and demonstrate that hemin inhibition of HRI is not directly competing with either ATP or eIF2α peptide substrates and is mediated predominantly by heme binding within the HRI kinase domain."

36063997

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"Since the data described above suggested that the NT-HBD likely plays a role in the mechanism through which hemin represses the activity of HRI, we examined the electrophoretic properties of ΔH-HRI an[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

16109458

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"Finally, we show that hemin inhibition of HRI is relatively independent of binding of both ATP and an eIF2α substrate peptide and is mediated predominantly by the HRI kinase domain."

36063997

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"HRI is constitutively active, and is inhibited by hemin (Berlanga et al., 1998; Igarashi et al., 2008; Yang et al., 1992)."

36055199

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"We found that the inhibition of HRI by hemin did not occur in the presence of Cytochrome c (Figure 6L)."

36055199

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"We found that the inhibition of HRI by hemin did not occur in the presence of Cytochrome c ( xref )."

36055199

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"Early studies using rabbit reticulocyte lysates revealed that the addition of hemin (heme) to the lysates reverses HRI-mediated translational inhibition ."

37327776

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"Currently, the binding of hemin is postulated to repress the activity of HRI through the induction of an intermolecular disulfide bond between subunits within dimeric HRI [20,21] ."

16109458

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"Heme-regulated inhibitor kinase (HRI) is activated by hemin during erythrocyte maturation and by arsenite, an inducer of oxidative stress."

18356035

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"This process can then be arrested by the addition of hemin, which represses the activity of HRI ( Fig. 1 A, repressed HRI)."

16109458

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"HRI inhibition by hemin occurs primarily through the kinase domain."