IndraLab

Statements

MTOR phosphorylates RPS6KB1 on T412. 92 / 133
2 5 1 2 | 50 42 29
reach
"As p70S6K (Thr389) could be directly phosphorylated by mTOR, we further confirmed the changes in mTOR/p70S6K (Thr389) by western blotting."
36526973
reach
"Down-regulation of mTOR dramatically decreased the mTOR kinase activity, since the basal or hsBAFF-stimulated phosphorylation of S6K1 at T389, routinely used as an indicator of mTOR kinase activity [21, 22], was not detectable by Western blotting (Fig. 8b)."
26118661
sparser
"Western blot studies showed that the expression and activation of mTOR were both inhibited by GL-V9 ( xref E), because mTOR specifically phosphorylates the p70S6 kinase at Thr-389, the phosphorylation of p70S6 kinase at this position is a routine and specific assay for monitoring mTOR activity [ xref ]."
33143000
sparser
"The activated mTOR phosphorylates ribosomal protein S6 kinase beta-1 (S6K1) at Thr389 in these neurons to reduce food intake, elevate the renal sympathetic nerve outflow, increase energy expenditure and protect against obesity [ xref ,  xref ]."
37724323
sparser
"This takes into consideration that mTOR directly phosphorylates p70S6K on Thr389 [12] ."
17892857
sparser
"MTOR phosphorylates S6K1 at Thr-389 (its hydrophobic motif), which, in turn, phosphorylates translational effectors such as rpS6 (ribosomal protein S6) and eIF4B (eukaryotic translation initiation factor 4B) [ xref , xref ]."
31195688
reach
"Finally, PKC δ is required during both ET-1 and insulin stimulation of S2448 phosphorylation of mTOR and subsequent T389 phosphorylation of S6K1 ( Figs."
17976640
sparser
"Another regulator of p70S6K is the mammalian target of rapamycin (mTOR) which also directly phosphorylate Thr-389 of p70S6K [ xref , xref ]."
32695569
sparser
"ERK phostorylates p70S6K at Thr 421 /Ser 424 , while mTOR phosphorylates p70S6K at Thr 389 ."
22751450
sparser
"Phosphorylation of p70s6k at Thr-389 by mTOR promotes the increased translation of mRNAs with a 5’-tract of pyrimidine."
19448694
sparser
"To further study how p53 affected S6K1, we compared pS6K1 Thr 389 which is phosphorylated by mTOR, Thr 421 /Ser 424 which is on the auto-inhibitory domain, Thr 229 which is phosphorylated by PDK1 [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
28315428
sparser
"The mTOR-dependent phosphorylation of p70 S6K1 at T389 is essential for its nuclear localization and exclusively hyperphosphorylated p70 S6K1 can be found in the nucleus."
21602892
sparser
"Furthermore, mTOR phosphorylates S6K1 on Thr 389 , a residue whose phosphorylation is associated with activation of the protein [26] ."
12387821
sparser
"Then, mTOR phosphorylates downstream p70S6K1 Thr389 and 4E‐BP1 Thr37/46 to activate the eukaryotic initiation cytokines eIF4B and eIF4E and promote protein synthesis and muscle growth. xref Additionally, there is evidence indicating that neurotrophin 3 impacts protein synthesis and metabolic reorganization in neurogenic muscle by activating the TrkC/AKT/mTORC1 pathway in muscle fibres, as opposed to myoblasts. xref "
37127279
sparser
"RAFT1 phosphorylates p70(S6k) on Thr-389, a residue whose phosphorylation is rapamycin-sensitive in vivo and necessary for S6 kinase activity."
9465032
sparser
"Therefore, the interaction between S6K1 and eIF3-PIC appears to be regulated by mTOR-dependent phosphorylation of S6K1 at T389."
16286006
sparser
"Unlike PF-4708671, RAME decreased the mTOR-dependent phosphorylation of S6K1 T389 in a dose-dependent manner (Fig. xref a, c)."
31387554
reach
"Because mTOR specifically phosphorylated the p70S6 kinase at Thr-389, use of western blotting to detect the phosphorylation level of p70S6K at this position was a common and specific assay for monitor[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
30995437
sparser
"Consistently, alkaline pHi increased the PI3K- and mTOR-dependent phosphorylation of 4EBP1 and S6K1 T389 in this experimental context."
37507012
reach
"We therefore hypothesized that the interaction between eIF3-PIC and S6K1 might be sensitive to mTOR modulated S6K1 T389 phosphorylation."
16286006
reach
"Thr389 phosphorylation of S6K1, which is modulated by mTOR, was increased."
33363046
sparser
"PHD3 knockdown also increased the phosphorylation of JNK at Thr 183 and Tyr 185 under basal and TNF-α-stimulated conditions (Fig.  xref ) but reduced mTOR complex 1-dependent phosphorylation of p70 S6 kinase at Thr 389 , Thr 421 , and Ser 424 (ref. xref ) under insulin stimulation (Supplementary Fig.  xref ), consistent with impaired insulin signalling."
30250231
reach
"Western blot studies showed that the expression and activation of mTOR were both inhibited by GL-V9 (XREF_FIG E), because mTOR specifically phosphorylates the p70S6 kinase at Thr 389, the phosphorylation of p70S6 kinase at this position is a routine and specific assay for monitoring mTOR activity [XREF_BIBR]."
33143000
reach
"MTOR phosphorylates and activates p70S6K at threonine 389 that serves as a marker of mTOR activity XREF_BIBR."
23029019
reach
"This effect, related to the TrkB-dependent activation of the mTOR which induces the phosphorylation of the p70S6K on T389 and T421/S424 ( Zhou et al., 2010 )."
23796670
reach
"MTOR phosphorylates p70S6K at Thr 389 in the regulatory domain in vivo and in vitro [49]."
15621776
reach
"The activated mTOR phosphorylates ribosomal protein S6 kinase beta-1 (S6K1) at Thr389 in these neurons to reduce food intake, elevate the renal sympathetic nerve outflow, increase energy expenditure and protect against obesity [114, 115]."
37724323
sparser
"MTOR, which specifically phosphorylates the p70 S6 kinase at Thr-389, negatively regulates autophagy ( Noda & Ohsumi, 1998 )."
17881280
reach
"Moreover, mTOR induces S6K1 (Thr 389) phosphorylation that promotes S6K1 nucleus translocation [43]."
28315428
reach
"S6K-1 is phosphorylated on several residues by mTOR (Thr 389 and Ser 371 ), but also by PDK1 (Thr 229 ), and these phosphorylations are required for its activity [18,19] ."
16516166
reach
"We then immunopurified HA-S6K1 after inhibitor and EGF stimulation and incubated them with immunopurified myc-mTOR to see whether mTOR can phosphorylate S6K1 at Thr 389 in the absence of Thr 229 phosphorylation by PDK1."
21561857
sparser
"Phosphorylation of p70 S6 kinase by mTOR at Thr-389 is thought to be involved in activation of the kinase, leading to phosphorylation of ribosomal protein S6, thus stimulating translation of ‘5′-TOP’ [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12829246
sparser
"The mTOR dependent Thr389 phosphorylation of S6K1 is the most frequently used marker for the S6K1 activity ( xref )."
30705751
sparser
"Because Thr389 on p70S6 kinase is phosphorylated by mTOR, 22 increase in its phosphorylation is used as an index of mTOR activation."
17991718
reach
"ERK phostorylates p70S6K at Thr 421 / Ser 424, while mTOR phosphorylates p70S6K at Thr 389."
22751450
sparser
"S6K1 is phosphorylated by activated mTOR on Thr389, which then phosphorylates S6K15, a component of the 40S ribosomal protein [ xref ]."
37513916
reach
"32 MTOR phosphorylates S6K1 at Thr389, and activated S6K1 further phosphorylates RPS6 to regulate protein translation, synthesis, and metabolism."
32764535
reach
"Since it was demonstrated that mTOR phosphorylates p70 S6K1 at T389 and that this phosphorylation plays an important physiological role in insulin signalling, analysis of the T389 phosphorylation of p[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18809440
reach
"Further downstream, mTOR phosphorylates p70 S6 kinase (T389), a regulator of Ribosomal Protein S6 (RPS6) responsible for mRNA translation, and has been proposed to signal via 14-3-3 (tyrosine 3-monooxygenase), a required protein in Raf signaling, to inhibit IRS-1 in a negative feedback loop XREF_BIBR."
21980324
reach
"P70S6 kinase is phosphorylated by PDK1 on thr 229 and by mTOR on thr 389."
12967624
reach
"[47] Following growth factor stimulation, S6K1 is phosphorylated by mTOR kinase at Thr229 on the activation loop and subsequently at Thr389 on the hydrophobic region by PDK1 [63]; once activated, S6K1 catalyzes the phosphorylation of the S6 ribosomal protein, thus playing a key role in the regulation of protein synthesis and cell growth."
26652182
sparser
"MTOR kinase phosphorylates S6K1 at T389 which is the hydrophobic motif (HM) phosphorylation site [ xref , xref ] followed by the PDK1 mediated phosphorylation at T229 present in the activation loop (AL) which leads to full activation of S6K1 [ xref ]."
25505994
reach
"The site at Thr389 is essential to the function of S6K1, and activated mTOR can phosphorylate S6K1 at the Thr389 residue, causing phosphorylation and recruitment of the 40S ribosomal unit and finally enhancing the translation of mRNAs, including elongation factors and ribosomal proteins XREF_BIBR."
25486097
sparser
"PmTOR phosphorylates p70S6 kinase at Thr-389, and immunoblot analysis to determine the level of phosphorylation at this position is a specific assay used to monitor mTOR activity [26] ."
17825886
sparser
"Because the inhibitory function of mTOR in autophagy has been well established [ xref ], the phosphorylation of p70S6K at Thr389 by mTOR was also evaluated to assess mTOR activity."
34226513
reach
"The S6K1 is phosphorylated at Thr 389 by mTOR XREF_BIBR."
25062042
reach
"Furthermore, mTOR phosphorylates S6K1 on Thr 389, a residue whose phosphorylation is associated with activation of the protein [26]."
12387821
sparser
"Upon activation, mTOR phosphorylates p70S6K at Thr389 and 4EBPs [there are three 4EBPs (4EBP1–3), and 4EBP2 is a major isoform in neurons] at Thr37/46 and Ser65 directly."
24795562
reach
"MTOR phosphorylates and activates p70S6K at T389 to activate the ribosomal protein S6 through phosphorylation at S235/236 and S240/244 (23)."
36137184
sparser
"We then immunopurified HA-S6K1 after inhibitor and EGF stimulation and incubated them with immunopurified myc-mTOR to see whether mTOR can phosphorylate S6K1 at Thr-389 in the absence of Thr-229 phosphorylation by PDK1."
21561857
sparser
"Since it was demonstrated that mTOR phosphorylates p70 S6K1 at T389 and that this phosphorylation plays an important physiological role in insulin signalling, analysis of the T389 phosphorylation of p[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18809440
sparser
"However, contrary to previous assumptions, the presence of raptor was not necessary for mTOR to phosphorylate p70 s6k T389."
23077579
reach
"Because mTOR specifically phosphorylates the p70S6 kinase at Thr-389, use of western blotting to determine the phosphorylation of p70S6 kinase at this position is a routine and specific assay for moni[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
22560876
sparser
"Activated mTOR phosphorylates p70S6K at Thr389 and finally induces systemic inflammatory gene expression ( Lim et al., 2003 )."
31863766
sparser
"Because mTOR specifically phosphorylated the p70S6 kinase at Thr-389, use of western blotting to detect the phosphorylation level of p70S6K at this position was a common and specific assay for monitor[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
30995437
reach
"PF-4708671 (PF) is a potent and selective inhibitor of p70S6K that induces an enhancement of the mTOR catalysed phosphorylation of p70S6K at Thr389 [XREF_BIBR]."
26919095
sparser
"Furthermore, we found that the overexpression of LPAAT-theta can induce mTOR-dependent p70S6K phosphorylation on Thr389 and 4EBP1 phosphorylation on Ser65 in HEK293T cells."
17002884
reach
"More specifically, activated mTOR, when phosphorylated at Ser2448, phosphorylates p70-S6K1 at Thr389 to stimulate translation capacity and hyper-phosphorylates eIF4E binding protein 1 (4E-BP1) to inhi[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
25496993
reach
"S6K1 short isoforms can not be phosphorylated by mTOR at Threonine 389 and by phosphoinositide dependent kinase 1 (PDK1) at Threonine 229, as they lack both phosphorylation sites."
23273915
reach
"Activated mTOR phosphorylates p70S6K at Thr389 and finally induces systemic inflammatory gene expression ( Lim et al., 2003 )."
31863766
reach
"MTOR kinase phosphorylates S6K1 at T389 which is the hydrophobic motif (HM) phosphorylation site [XREF_BIBR, XREF_BIBR] followed by the PDK1 mediated phosphorylation at T229 present in the activation loop (AL) which leads to full activation of S6K1 [XREF_BIBR]."
25505994
reach
"In addition, mTOR can also phosphorylate and activate p70S6K at threonine 412, which in turn can inhibit the eukaryotic elongation factor 2 kinase (eEF2K), thereby allowing peptide elongation to resum[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
32114078
sparser
"Therefore effects of E Platinum on the expression of mTOR and its phosphorylation product p-mTOR (Ser2448) were examined since mTOR specifically phosphorylates the p70S6 kinase at Thr-389."
22322152
sparser
"Recent data have shown that in mid G1 phase, p70S6K1 becomes phosphorylated by mTOR at T389 and translocated to the nucleus [ xref ]."
26698305
reach
"Similarly, mTOR inhibition by rapamycin resulted in decreased phosphorylation of target protein, p70S6K at Thr 389 and enhanced autophagic effect of alpha-solanine (XREF_FIG)."
26313911
reach
"MTOR phosphorylates the 70-kDa ribosomal protein S6 kinase (p70S6K) at T389 and the activated p70S6K in turn phosphorylates the ribosomal protein S6 (rpS6) at S235/236, which initiates mRNA translation."
33311466
reach
"A widely used method for assessing mTOR activity is by measuring the steady-state levels of downstream targets directly phosphorylated by mTOR, such as p70S6K phosphorylated by mTOR at threonine 389, and 4EBP1 phosphorylated by mTOR at threonine 37/46."
24411482
reach
"The mTOR dependent phosphorylation of p70 S6K1 at T389 is essential for its nuclear localization and exclusively hyperphosphorylated p70 S6K1 can be found in the nucleus."
21602892
reach
"Down-regulating mTOR and raptor reduced S6K1 phosphorylation at Thr389 in one-cell embryos."
19877273
reach
"MTOR phosphorylates p70 S6k1 on Thr389, an event that is critical for maximal activation of the kinase."
15659336
sparser
"As the phosphorylated mTOR can phosphorylate p70 ribosomal S6 kinase (p70S6k) at the Thr389 site ( Wang et al., 2001 ), further experiment was designed to examine the p70S6k activity in the hippocampu[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
20167228
reach
"MTOR phosphorylates S6K1 at T389 to enhance S6K1 activity, which amongst other things phosphorylates the S6 subunit of the ribosome to promote translation."
20169205
reach
"Furthermore, recent study has indicated that mTOR forms a complex with raptor, the regulatory associated protein of mTOR, in response to the nutrient-dependent signal, and that mTOR/raptor phosphoryla[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17892857
reach
"MTOR inhibition with its ATP-competitive inhibitor INK128 led to the complete dephosphorylation of S6K1 (Thr389) and 4E-BP1 (Ser65) proteins."
31116686
reach
"S6K1 is phosphorylated by activated mTOR on Thr389, which then phosphorylates S6K15, a component of the 40S ribosomal protein [63]."
37513916
sparser
"MTOR phosphorylates S6K1 at T389 to enhance S6K1 activity, which amongst other things phosphorylates the S6 subunit of the ribosome to promote translation."
20169205
reach
"In this context, activation of mTORC1 leads to increased translation and cell growth by two mechanisms : first, phosphorylation of Thr 389 of S6K1 by mTOR results in activation of S6K1 and subsequent phosphorylation of five evolutionarily conserved residues (Ser235, Ser236, Ser240, Ser244, and Ser247) of ribosomal protein S6 (RPS6) that activates RPS6."
21977024
sparser
"Because mTOR specifically phosphorylates the p70S6 kinase at Thr-389, use of western blotting to determine the phosphorylation of p70S6 kinase at this position is a routine and specific assay for moni[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
22560876
sparser
"Activation of mTOR by mitogens and growth factors leads to phosphorylation of S6K1 by mTOR at the hydrophobic motif site T389 within the catalytic domain, a critical event in S6K1 activation ( xref )."
19302577
reach
"MTOR phosphorylates and activates p70S6K at threonine 389 which serves as a marker of mTOR activity."
22873724
sparser
"S6K1 is located in both cytoplasm and nucleus and the mTOR-dependent phosphorylation of S6K1 at Thr389 is essential for nuclear localization [ 6 ]."
33278383
reach
"1 In addition to PDK1, mTOR is also involved in the activation of S6K1 2 and phosphorylates S6K1 at residue T412."
25356520
reach
"Therefore effects of E Platinum on the expression of mTOR and its phosphorylation product p-mTOR (Ser2448) were examined since mTOR specifically phosphorylates the p70S6 kinase at Thr-389."
22322152
reach
"MTOR phosphorylates ribosomal p70S6 kinase 1 (p70S6K) at its hydrophobic motif site, T389; in turn, the phosphorylated p70S6K phosphorylates its substrate, the S6 ribosomal protein at S235/236."
31409554
reach
"Upon activation, mTOR phosphorylates p70S6K at Thr389 and 4EBPs [there are three 4EBPs (4EBP1-3), and 4EBP2 is a major isoform in neurons] at Thr37/46 and Ser65 directly."
24795562
sparser
"MTOR phosphorylates p70 S6k1 on Thr389, an event that is critical for maximal activation of the kinase."
15659336
sparser
"However, it has recently been reported that ULK1 expression is upregulated to protect against MPP + -induced MN9D cell vulnerability via inhibition of mTOR kinase-induced T389 phosphorylation and activation of S6k1 [ xref ]."
30740222
reach
"MTOR immunopurified from culture cells or tissues phosphorylates in vitro p70 S6 kinase (p70) alpha and p70beta, mainly on Thr412 or Thr401, respectively, located in a Phe-Thr-Tyr motif."
14560963
reach
"Further, mTOR phosphorylates and activates S6K1 at Thr389, which promotes translation of cell proliferation-related mRNA [66]."
36834509
reach
"MTOR phosphorylates Thr 389 of S6K1 in the regulatory T loop, an event required for S6K1 activation."
28242756
sparser
"It has already been confirmed that the S6K1 protein is located in both cytoplasm and nucleus and the mTOR-dependent phosphorylation of S6K1 at Thr389 is essential for its nuclear localization [ 6 ]."
33278383
sparser
"S6K1 is phosphorylated by mTOR at Ser389."
| PMC