IndraLab

Statements

MTOR phosphorylates RPS6KB1 on T412. 76 / 78
2 5 1 2 | 32 24 10
rlimsp
"MTOR phosphorylates and activates p70S6K at threonine389 that serves as a marker of mTOR activity [56]."
23029019
reach
"A widely used method for assessing mTOR activity is by measuring the steady-state levels of downstream targets directly phosphorylated by mTOR, such as p70S6K phosphorylated by mTOR at threonine 389, and 4EBP1 phosphorylated by mTOR at threonine 37/46."
24411482
sparser
"Furthermore, we found that the overexpression of LPAAT-theta can induce mTOR-dependent p70S6K phosphorylation on Thr389 and 4EBP1 phosphorylation on Ser65 in HEK293T cells."
17002884
sparser
"However, it has recently been reported that ULK1 expression is upregulated to protect against MPP + -induced MN9D cell vulnerability via inhibition of mTOR kinase-induced T389 phosphorylation and activation of S6k1 [ xref ]."
30740222
rlimsp
"Multiple phosphorylation sites have also been found on the serine and threonine residues of p70S6K, however, phosphorylation of threonine 389 residue by mTOR is critical for p70S6K activation and serves as a marker for mTOR activity [87]."
21307646
reach
"1 In addition to PDK1, mTOR is also involved in the activation of S6K1 2 and phosphorylates S6K1 at residue T412."
25356520
reach
"Western blot studies showed that the expression and activation of mTOR were both inhibited by GL-V9 (XREF_FIG E), because mTOR specifically phosphorylates the p70S6 kinase at Thr 389, the phosphorylation of p70S6 kinase at this position is a routine and specific assay for monitoring mTOR activity [XREF_BIBR]."
33143000
rlimsp
"We then immunopurified HA-S6K1 after inhibitor and EGF stimulation and incubated them with immunopurified myc-mTOR to see whether mTOR can phosphorylate S6K1 at Thr-389 in the absence of Thr-229 phosphorylation by PDK1."
21561857
sparser
"Recent data have shown that in mid G1 phase, p70S6K1 becomes phosphorylated by mTOR at T389 and translocated to the nucleus [ xref ]."
26698305
reach
"Further downstream, mTOR phosphorylates p70 S6 kinase (T389), a regulator of Ribosomal Protein S6 (RPS6) responsible for mRNA translation, and has been proposed to signal via 14-3-3 (tyrosine 3-monooxygenase), a required protein in Raf signaling, to inhibit IRS-1 in a negative feedback loop XREF_BIBR."
21980324
reach
"P70S6K is phosphorylated by FRAP and mTOR downstream of Akt at Thr 389 and at Thr 421 / Ser 42, downstream of the Ras-Raf-MEK-ERK pathway."
15631631
reach
"P70S6K is phosphorylated by FRAP/mTOR downstream of Akt at Thr389 and at Thr421/Ser42, downstream of the Ras-Raf-MEK-ERK pathway."
15631631
sparser
"Another regulator of p70S6K is the mammalian target of rapamycin (mTOR) which also directly phosphorylate Thr-389 of p70S6K [ xref , xref ]."
32695569
signor
"We report here that a mammalian recombinant p70alpha polypeptide, extracted in an inactive form from rapamycin-treated cells, can be directly phosphorylated by the mTOR kinase in vitro predominantly at the rapamycin-sensitive site Thr-412. mTOR-catalyzed p70alpha phosphorylation in vitro is accompanied by a substantial restoration in p70alpha kinase activity toward its physiologic substrate"
10567431
sparser
"MTOR phosphorylates S6K1 at Thr-389 (its hydrophobic motif), which, in turn, phosphorylates translational effectors such as rpS6 (ribosomal protein S6) and eIF4B (eukaryotic translation initiation factor 4B) [ xref , xref ]."
31195688
sparser
"Unlike PF-4708671, RAME decreased the mTOR-dependent phosphorylation of S6K1 T389 in a dose-dependent manner (Fig. xref a, c)."
31387554
phosphoelm
No evidence text available
15809305
signor
"S6K1 is a positive regulator of protein synthesis, and its activity is induced by mTOR-mediated phosphorylation."
12782654
reach
"ERK phostorylates p70S6K at Thr 421 / Ser 424, while mTOR phosphorylates p70S6K at Thr 389."
22751450
reach
"S6K1 short isoforms can not be phosphorylated by mTOR at Threonine 389 and by phosphoinositide dependent kinase 1 (PDK1) at Threonine 229, as they lack both phosphorylation sites."
23273915
biopax:phosphositeplus
No evidence text available
reach
"MTOR phosphorylates and activates p70S6K at threonine 389 that serves as a marker of mTOR activity XREF_BIBR."
23029019
reach
"MTOR phosphorylates S6K1 at T389 to enhance S6K1 activity, which amongst other things phosphorylates the S6 subunit of the ribosome to promote translation."
20169205
reach
"Thr389 phosphorylation of S6K1, which is modulated by mTOR, was increased."
33363046
sparser
"Furthermore, mTOR phosphorylates S6K1 on Thr 389 , a residue whose phosphorylation is associated with activation of the protein [26] ."
12387821
reach
"P70S6 kinase is phosphorylated by PDK1 on thr 229 and by mTOR on thr 389."
12967624
sparser
"We then immunopurified HA-S6K1 after inhibitor and EGF stimulation and incubated them with immunopurified myc-mTOR to see whether mTOR can phosphorylate S6K1 at Thr-389 in the absence of Thr-229 phosphorylation by PDK1."
21561857
reach
"The site at Thr389 is essential to the function of S6K1, and activated mTOR can phosphorylate S6K1 at the Thr389 residue, causing phosphorylation and recruitment of the 40S ribosomal unit and finally enhancing the translation of mRNAs, including elongation factors and ribosomal proteins XREF_BIBR."
25486097
rlimsp
"This proposal is not supported, however, by the existence of mutants of S6K1 that are phosphorylated in vivo on Thr(389) in a rapamycin-resistant fashion. Here, we demonstrate that the raptor-mTOR complex phosphorylates the rapamycin-sensitive forms of S6K1, while the distinct rictor-mTOR complex phosphorylates the rapamycin-resistant mutants of S6K1. Phosphorylation of Thr(389) by rictor-mTOR is independent of the TOR signaling motif and depends on removal of the carboxyl terminal domain of S6K1."
15809305
sparser
"S6K1 is phosphorylated by mTOR at Ser389."
| PMC
reach
"MTOR phosphorylates Thr 389 of S6K1 in the regulatory T loop, an event required for S6K1 activation."
28242756
signor
"MTORC1 catalyzes the phosphorylation of eIF4E binding protein-1 (4EBP1, also known as PHAS-I) and p70 S6 kinase 1 (S6K1)Phosphorylation of S6K1 at Thr-389"
17510057
rlimsp
"We then immunopurified HA-S6K1 after inhibitor and EGF stimulation and incubated them with immunopurified myc-mTOR to see whether mTOR can phosphorylate S6K1 at Thr-389 in the absence of Thr-229 phosphorylation by PDK1. As shown in Fig. 5b, only pretreatment with PDK1 leads to phosphorylation of Thr-389 by mTOR, suggesting that Thr-389 is followed by Thr-229."
21561857
sparser
"MTOR kinase phosphorylates S6K1 at T389 which is the hydrophobic motif (HM) phosphorylation site [ xref , xref ] followed by the PDK1 mediated phosphorylation at T229 present in the activation loop (AL) which leads to full activation of S6K1 [ xref ]."
25505994
signor
"S6 kinases are under the control of the PI3K relative, mammalian Target Of Rapamycin (mTOR), which may serve an additional function as a checkpoint for amino acid availability."
10579915
reach
"Similarly, mTOR inhibition by rapamycin resulted in decreased phosphorylation of target protein, p70S6K at Thr 389 and enhanced autophagic effect of alpha-solanine (XREF_FIG)."
26313911
sparser
"Therefore, the interaction between S6K1 and eIF3-PIC appears to be regulated by mTOR-dependent phosphorylation of S6K1 at T389."
16286006
signor
"Collectively, these results indicate that Arg, Leu, and Gln act coordinately to stimulate proliferation of pTr cells through activation of the MTOR-RPS6K-RPS6-EIF4EBP1 signal transduction pathway"
23486913
reach
"The S6K1 is phosphorylated at Thr 389 by mTOR XREF_BIBR."
25062042
reach
"MTOR kinase phosphorylates S6K1 at T389 which is the hydrophobic motif (HM) phosphorylation site [XREF_BIBR, XREF_BIBR] followed by the PDK1 mediated phosphorylation at T229 present in the activation loop (AL) which leads to full activation of S6K1 [XREF_BIBR]."
25505994
rlimsp
"We found that the effects of rapamycin were specific to inhibition of mTOR/p70S6K, since rapamycin treatment had no significant effect of phospho-ACC levels (Fig. 5A), indicating no effect on AMPK activity, but, rather, significantly inhibited phosphorylation of p70S6K on Thr 389 (Fig. 5B), which is the mTOR phosphorylation site critical for kinase function (Saitoh et al., 2002)."
27856330
hprd
No evidence text available
12604610
sparser
"Western blot studies showed that the expression and activation of mTOR were both inhibited by GL-V9 ( xref E), because mTOR specifically phosphorylates the p70S6 kinase at Thr-389, the phosphorylation of p70S6 kinase at this position is a routine and specific assay for monitoring mTOR activity [ xref ]."
33143000
rlimsp
"In this context, activation of mTORC1 leads to increased translation and cell growth by two mechanisms: first, phosphorylation of Thr389 of S6K1 by mTOR results in activation of S6K1 and subsequent phosphorylation of five evolutionarily conserved residues (Ser235, Ser236, Ser240, Ser244, and Ser247) of ribosomal protein S6 (RPS6) that activates RPS6."
21977024
reach
"MTOR phosphorylates and activates p70S6K at threonine 389 which serves as a marker of mTOR activity."
22873724
reach
"Upon activation, mTOR phosphorylates p70S6K at Thr389 and 4EBPs [there are three 4EBPs (4EBP1-3), and 4EBP2 is a major isoform in neurons] at Thr37/46 and Ser65 directly."
24795562
sparser
"Because the inhibitory function of mTOR in autophagy has been well established [ xref ], the phosphorylation of p70S6K at Thr389 by mTOR was also evaluated to assess mTOR activity."
34226513
biopax:phosphositeplus
No evidence text available
phosphoelm
No evidence text available
15718470
reach
"In this context, activation of mTORC1 leads to increased translation and cell growth by two mechanisms : first, phosphorylation of Thr 389 of S6K1 by mTOR results in activation of S6K1 and subsequent phosphorylation of five evolutionarily conserved residues (Ser235, Ser236, Ser240, Ser244, and Ser247) of ribosomal protein S6 (RPS6) that activates RPS6."
21977024
reach
"Furthermore, mTOR phosphorylates S6K1 on Thr 389, a residue whose phosphorylation is associated with activation of the protein [26]."
12387821
reach
"Down-regulating mTOR and raptor reduced S6K1 phosphorylation at Thr389 in one-cell embryos."
19877273
sparser
"However, contrary to previous assumptions, the presence of raptor was not necessary for mTOR to phosphorylate p70 s6k T389."
23077579
reach
"PF-4708671 (PF) is a potent and selective inhibitor of p70S6K that induces an enhancement of the mTOR catalysed phosphorylation of p70S6K at Thr389 [XREF_BIBR]."
26919095
sparser
"PHD3 knockdown also increased the phosphorylation of JNK at Thr 183 and Tyr 185 under basal and TNF-α-stimulated conditions (Fig.  xref ) but reduced mTOR complex 1-dependent phosphorylation of p70 S6 kinase at Thr 389 , Thr 421 , and Ser 424 (ref. xref ) under insulin stimulation (Supplementary Fig.  xref ), consistent with impaired insulin signalling."
30250231
reach
"32 MTOR phosphorylates S6K1 at Thr389, and activated S6K1 further phosphorylates RPS6 to regulate protein translation, synthesis, and metabolism."
32764535
sparser
"RAFT1 phosphorylates p70(S6k) on Thr-389, a residue whose phosphorylation is rapamycin-sensitive in vivo and necessary for S6 kinase activity."
9465032
sparser
"To further study how p53 affected S6K1, we compared pS6K1 Thr 389 which is phosphorylated by mTOR, Thr 421 /Ser 424 which is on the auto-inhibitory domain, Thr 229 which is phosphorylated by PDK1 [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
28315428
reach
"We therefore hypothesized that the interaction between eIF3-PIC and S6K1 might be sensitive to mTOR modulated S6K1 T389 phosphorylation."
16286006
rlimsp
"The site at Thr389 is essential to the function of S6K1, and activated mTOR can phosphorylate S6K1 at the Thr389 residue, causing phosphorylation and recruitment of the 40S ribosomal unit and finally enhancing the translation of mRNAs, including elongation factors and ribosomal proteins [31]."
25486097
reach
"We then immunopurified HA-S6K1 after inhibitor and EGF stimulation and incubated them with immunopurified myc-mTOR to see whether mTOR can phosphorylate S6K1 at Thr 389 in the absence of Thr 229 phosphorylation by PDK1."
21561857
reach
"Down-regulation of mTOR dramatically decreased the mTOR kinase activity, since the basal or hsBAFF-stimulated phosphorylation of S6K1 at T389, routinely used as an indicator of mTOR kinase activity [21, 22], was not detectable by Western blotting (Fig. 8b)."
26118661
sparser
"Upon activation, mTOR phosphorylates p70S6K at Thr389 and 4EBPs [there are three 4EBPs (4EBP1–3), and 4EBP2 is a major isoform in neurons] at Thr37/46 and Ser65 directly."
24795562
reach
"Moreover, mTOR induces S6K1 (Thr 389) phosphorylation that promotes S6K1 nucleus translocation [43]."
28315428
rlimsp
"RAFT1 phosphorylates p70(S6k) on Thr-389, a residue whose phosphorylation is rapamycin-sensitive in vivo and necessary for S6 kinase activity."
9465032
rlimsp
"As phosphorylation of Thr(389) is rapamycin sensitive and mTOR can phosphorylate the same site in vitro, it has been suggested that mTOR is the physiological Thr(389) kinase. This proposal is not supported, however, by the existence of mutants of S6K1 that are phosphorylated in vivo on Thr(389) in a rapamycin-resistant fashion."
15809305
sparser
"The mTOR dependent Thr389 phosphorylation of S6K1 is the most frequently used marker for the S6K1 activity ( xref )."
30705751
sparser
"The mTOR-dependent phosphorylation of p70 S6K1 at T389 is essential for its nuclear localization and exclusively hyperphosphorylated p70 S6K1 can be found in the nucleus."
21602892
sparser
"ERK phostorylates p70S6K at Thr 421 /Ser 424 , while mTOR phosphorylates p70S6K at Thr 389 ."
22751450
sparser
"Phosphorylation of p70 S6 kinase by mTOR at Thr-389 is thought to be involved in activation of the kinase, leading to phosphorylation of ribosomal protein S6, thus stimulating translation of ‘5′-TOP’ [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12829246
reach
"The mTOR dependent phosphorylation of p70 S6K1 at T389 is essential for its nuclear localization and exclusively hyperphosphorylated p70 S6K1 can be found in the nucleus."
21602892
sparser
"Activation of mTOR by mitogens and growth factors leads to phosphorylation of S6K1 by mTOR at the hydrophobic motif site T389 within the catalytic domain, a critical event in S6K1 activation ( xref )."
19302577
reach
"MTOR phosphorylates ribosomal p70S6 kinase 1 (p70S6K) at its hydrophobic motif site, T389; in turn, the phosphorylated p70S6K phosphorylates its substrate, the S6 ribosomal protein at S235/236."
31409554
sparser
"MTOR phosphorylates S6K1 at T389 to enhance S6K1 activity, which amongst other things phosphorylates the S6 subunit of the ribosome to promote translation."
20169205
reach
"MTOR immunopurified from culture cells or tissues phosphorylates in vitro p70 S6 kinase (p70) alpha and p70beta, mainly on Thr412 or Thr401, respectively, located in a Phe-Thr-Tyr motif."
14560963
reach
"MTOR phosphorylates the 70-kDa ribosomal protein S6 kinase (p70S6K) at T389 and the activated p70S6K in turn phosphorylates the ribosomal protein S6 (rpS6) at S235/236, which initiates mRNA translation."
33311466