IndraLab
Statements
sparser
"The HET-CAM test revealed that Soluplus and Pluronic F68 micelle dispersions behaved as the negative control (0.9% NaCl) without inducing bleeding, lysis or coagulation at the time of the study ( t H , t L , and t C > 301 s; IS = 0), which means that they can be considered non-irritants ( xref )."
sparser
"Regarding the biosafety properties of the mini-implant, current in vitro and in vivo evaluations have shown that the sample extraction medium did not induce impairment of the HGF cell viable population or irritative potential when using the HET-CAM protocol, supporting thus the use of the custom-made mini-implant alloy in further experimental clinical applications."
sparser
"The potential irritancy was detected by observing adverse changes which occur in the CAM of the hen’s egg, after exposure to both hydro-alcoholic extracts obtained by aerial parts and leaves of Ocimum basilicum L. Correlating with the lack of severe cytotoxicity on skin-derived cell lines and on the in vivo skin measurements, the results obtained in the HET-CAM assay ( xref and xref ) confirms the biocompatibility and good tolerance of both basil hydro-alcoholic extracts on the vascular developing plexus."
sparser
"To estimate its stability, major long-lived species were quantified across several weeks of storage at −20 • C. Subsequently, its toxic effects against of human malignant cell lines of the peritoneal cavity was investigated in both 2D and 3D models, including the HET-CAM chicken embryo vascularized tumor model."
sparser
"The two most potent α-glucosidase inhibitors, aqueous extracts from Geum urbanum L. (avens root) and Rhodiola rosea L. (roseroot) were further examined by in-vitro enzyme and cell culture-based assays as well as by in-ovo (hen’s egg test-chorioallantoic membrane; HET-CAM) and in-vivo models ( D. melanogaster ) to pinpoint potential antidiabetic activities."
sparser
"For this purpose, the HET-CAM has gained acceptance as an alternative to the rabbit eye irritation test (Draize test) for the assessment of the mucous membrane irritation potential (Barile xref ), and has been extensively used to evaluate the irritation potential of different substances and products (Derouiche and Abdennour xref ; Marquardt et al. xref ; McKenzie et al. xref ; Rajpal Deshmukh et al. xref ; Scheel et al. xref ; Steiling et al. xref )."
sparser
"The investigation has initiated with the evaluation of inhibiting the angiogenesis which is a main activity in metastasis, and it was assessed through Hen’s Egg Test on Chorio Allantoic Membrane (HET-CAM) test; the BSM inhibited the growth of blood vessels in the developing chick embryo."
sparser
"Safety of the developed formulation as a carrier for ophthalmic drug delivery was measured using three different methods: MTT assay for measuring cell viability in which the human retinal pigmentation epithelial cells (RPE) were used, HET-CAM as a borderline method between in vivo and in vitro techniques for investigating the irritation potential of the chosen formulation which was done by adding formulation directly on the CAM surface and visually monitoring the vessels in terms of irritation reactions, and finally the modified Draize test for evaluating tolerability of the selected formulation on eyes."
sparser
"Implants were manufactured by a hot-molding technique, characterized by Fourier Transform Infrared Spectroscopy, X-Ray Diffraction, Differential Scanning Calorimetry, Scanning Electron Microscopy; evaluated in terms of ocular biocompatibility by immunofluorescence, flow cytometry, cell migration, Hen's egg test-chorioallantoic membrane (HET-CAM) irritation test; and investigated in terms of in vitro efficacy against Toxoplasma gondii ."
sparser
"In this study, we focused on the evaluation of the cytotoxic profile of ethinylestradiol (EE), levonorgestrel (LNG), and their association (EE + LNG), with and without UVB irradiation, on healthy cell lines (human keratinocytes, fibroblasts, and primary epidermal melanocytes, and mouse epidermis cells) and tumor cell lines (human and murine melanoma) by in vitro (viability, migration and proliferation) and in vivo (HET-CAM) techniques."
sparser
"This study reports the effects of ASE-loaded nanoemulsions (NE ASE ) on the cellular viability, death by necrosis, and migration of immortalized human keratinocytes (HaCaT cell line), as well as the irritant potential through the hen's egg chorioallantoic membrane test (HET-CAM)."
sparser
"The non-irritant formulation, Mox-mPH NPs (1:150) (proven by HET-CAM test) exhibited intense antimicrobial activity against P. aeruginosa, S. pneumoniae, and S. aureus in vitro analyzed by live-dead cells assay, zone of inhibition studies, and by determining the minimum inhibitory and bactericidal concentrations."
sparser
"Results: The results correlate the composition and topography of the implant surface with biological experimental evaluations related to directly affected cells (gingival fibroblasts) and toxicological results on blood vessels (hen’s egg test-chorioallantoic membrane (HET-CAM) assay)."
sparser
"Since the HET-CAM test has been suggested as a valid method reliably applied in a research on potentially toxic substances [ xref ], it could be concluded that O. sanctum ethanolic extract both in the solution and nanodelivery system had no toxicity and was safe for topical applications."
sparser
"Before extending the analysis on human volunteers, the safety of Micah was assessed by the usual tests: genotoxicity (Ames test), ocular irritation (HET-CAM), phototoxicity (MTT ex vivo and in vivo on 22 volunteers), and sensitization (human repeated insult patch test on 50 volunteers)."
sparser
"HET-CAM assay was used in order to assess the toxicity of compounds 6 and 7 ( xref ); the procedure is a low-cost, unique model in biomedical research [ xref ] that allows the testing of the irritation potency of chemical compounds while simultaneously avoiding in vivo experiments [ xref ]."
| PMC
sparser
"In this feasibility study we demonstrated successful imaging and analysis of target-specific radiotracer accumulation in a HET-CAM xenograft model by combined PET and MRI, using the PSMA-specific PET tracer [ 68 Ga]Ga-PSMA-11 and PSMA-positive and PSMA-negative tumor cell lines."
sparser
"The finding that the selected nanoemulsion (containing tributyrin) displays some cytotoxicity against normal cells (although less pronounced compared to cancer cells) prompted us to assess the nanoemulsion potential for causing irritation to the administration site using HET-CAM."
sparser
"Imprinted and non-imprinted hydrogels were prepared with various contents in the functional monomer methacrylic acid (MAA) and characterized in terms of swelling, transmittance, mechanical properties, and ocular compatibility (hen’s egg test on chorioallantoic membrane (HET-CAM) assay)."
sparser
"Although the current form of the HET-CAM test is a valuable prescreen method for predicting the ocular irritation potential of chemicals, and can be used for reducing the number of experimental animals, a number of technical problems must still be addressed before these systems can replace whole animal tests."
sparser
"After having carried out toxicity studies as bovine corneal opacity and permeability (BCOP) and Heńs Egg Test - Chorioallantoic Membrane Test (HET-CAM) it was verified that both, the active ingredients and the excipients of the different formulations were not harmful without generating irritation, loss of transparency or corneal permeability alterations."
sparser
"Compared with other alternative tests, the HET-CAM test has some good points, such as could be carried out for all types of chemical substance (liquid or powder), applied with the similar condition of in vivo test, easy to access for research, not expensive, not need complicated animal room facility, and simple method [ xref , xref ]."
sparser
"Table 1: Parameters of texture analyzer used to measure the mucoadhesion of the prepared formulations with goat cornea
Table 2: Summary of the batches of Posaconazole nanosuspension as suggested by the DoE software
Table 3: HET-CAM potential irritancy score and inference deduced based on the time required for the endpoints to develop
Table 4: Zone of inhibition of different groups in the antifungal assay
Table 5: Characterization and stability results of the Posaconazole nanosuspension
Fig. 1: Forced degradation chromatograms of Posaconazole."
sparser
"HET-CAM was considered important in assessing the ocular irritation potential and, despite classifying almost all surfactants as "severe irritants", it could discriminate moderate and slight irritant SLES concentrations as well as Cocoamidopropyl Betaine as a severe irritant, when the coagulation score was taken into consideration."
sparser
"Based on the similarities of vasoactive effects observed in HET-CAM test, we suggest that the venom from B. arietans and B. parviocula may share certain venom protein families’ responsible for haemotoxicity, such as metalloproteinases and disintegrins, C-type lectins and snaclecs."
sparser
"HET-CAM is a well-accepted ex vivo conjunctival irritation model that produces responses (of hyperemia, hemorrhage, and clotting/coagulation) to test substances similar to those involving the conjunctiva of the eye. xref shows developmental stages of the CAM at 3- and 10-days and different irritant responses to the moderate irritant propylene glycol (PG), a strong irritant (sodium hydroxide 1 M), and F-P3/CS1. xref shows the cumulative numerical irritation scores of the test substances and controls."
sparser
"The favorable zero-order kinetics that prolonged capsaicin release and the significantly faster transdermal penetration of the NLC attributed to the reduction in skin irritation of the concentrated capsaicin NLCs, as illustrated by the in vitro EpiDerm TM three-dimensional human skin irritation test and hen’s egg test chorioallantoic membrane assay (HET-CAM)."
sparser
"With the capability of MRI to provide multiple image contrasts even in the small HET-CAM tumors [ xref ], its potential to provide further important physiological and molecular information (e.g., perfusion, diffusion, and tissue relaxation properties) for a more detailed tumor characterization is of major interest for future studies."
sparser
"We assayed the effects of venoms from the most widespread venomous African Bitis arietens and closely related species Bitis parviocula using The Hen’s Egg Test—Chorioallantoic membrane test (HET-CAM) and Chicken embryotoxicity screening test (CHEST), acetylcholinesterase (AChE) analysis, cytotoxicity assay performed on cell lines and protein analysis of selected venoms."
sparser
"In vitro SUN release profiles were obtained for the optimal formulations, along with ex vivo corneal permeability/retention studies, and ocular tolerance assays, namely: the bovine corneal opacity and permeability (BCOP) assay, the HET-CAM test (hen's egg test - chorioallantoic membrane), and hemolytic potential (HP) assay."
sparser
"Irritation tests of the chili extract-loaded lipid-based nanoparticle and the gel formulation containing the chili extract-loaded lipid nanocarrier were performed using the hen’s egg test chorioallantoic membrane (HET-CAM) assay and the in vitro skin irritation EpiDerm TM test model, respectively."
sparser
"Among them, cell and tissue culture models, the hen’s egg test on the chorioallantoic membrane (HET-CAM) as well as the Caenorhabditis elegans ( C. elegans ) model have gained considerable attention as alternative screening platforms to evaluate the toxicity of bioactive compounds in various context (Haselgrubler et al. xref ; Hunt xref ; Piersma xref ; Scheel et al. xref )."
sparser
"Thus, this study proposed to apply two strategies for the prediction of the eye irritation potential of different concentrations of surfactants and silicones, the first one involving evaluation Hen's Egg Test - Chorioallantoic Membrane (HET-CAM), and the other one using Bovine Corneal Opacity and Permeability (BCOP) followed by histopathological."
sparser
"The non-irritant behavior of the Soluplus and Pluronic F68 micelle dispersions is in good agreement with previous reports on HET-CAM assay results recorded for 23% w / v Soluplus dispersions [ xref ] and in situ gel formulations prepared with 23% w / v Pluronic F127 and 15% w / v Pluronic F68 [ xref ]."