IndraLab
Statements
CALM1 binds SAFB. 1000 / 1341
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1341
sparser
"The T14diLys/DOPE formulations with DNA showed excellent biocompatibility tested with the Hen’s egg test on the Chorioallantonic Membrane test (HET-CAM), and no charge-related toxic effects of the lipoplexes at the nitrogen-to-phosphate ratio (N/P ratio) 4 were observed in vitro."
sparser
"HET-CAM assay was used in order to assess the toxicity of compounds 6 and 7 ( xref ); the procedure is a low-cost, unique model in biomedical research [ xref ] that allows the testing of the irritation potency of chemical compounds while simultaneously avoiding in vivo experiments [ xref ]."
| PMC
sparser
"Implants were manufactured by a hot-molding technique, characterized by Fourier Transform Infrared Spectroscopy, X-Ray Diffraction, Differential Scanning Calorimetry, Scanning Electron Microscopy; evaluated in terms of ocular biocompatibility by immunofluorescence, flow cytometry, cell migration, Hen's egg test-chorioallantoic membrane (HET-CAM) irritation test; and investigated in terms of in vitro efficacy against Toxoplasma gondii ."
sparser
"The 3Rs principle based on replacement, reduction,
and refinement was derived from this directive and set the focus on
the use of alternative methods. xref As early
as 1985, HET-CAM contributed to the 3Rs principle and is now acknowledged
as an alternative test option to the Draize test. xref It can be used to investigate the potential for irritation
and the biocompatibility of a formulation."
sparser
"The CIP@GC(PLA) (NPs) (1:1) realized the highest association with human corneal epithelial cells, which were nonirritant to the hen's egg-chorioallantoic membrane test (HET-CAM test) and demonstrated significant antibacterial response in the in vitro minimum inhibitory, bactericidal, live-dead cells, zone of inhibition, and biofilm inhibition assays against the keratitis-inducing pathogen Pseudomonas aeruginosa ."
sparser
"We used a lower range of concentrations in the HET-CAM test (0.5 %, 1.0 %, and 2 % EKGR, for the vascular irritation test) and in the rabbits (0.25 %, 0.5 %, and 1.0 %, for acute dermal irritation test), as well as a higher range of doses in rats (0.5 %, 1.0 %, 2.0 %, and 4.0 %) and single-dose in rabbits (pure EKGR 200 mg/kg BW)."
sparser
"Before extending the analysis on human volunteers, the safety of Micah was assessed by the usual tests: genotoxicity (Ames test), ocular irritation (HET-CAM), phototoxicity (MTT ex vivo and in vivo on 22 volunteers), and sensitization (human repeated insult patch test on 50 volunteers)."
sparser
"The potential irritancy was detected by observing adverse changes which occur in the CAM of the hen’s egg, after exposure to both hydro-alcoholic extracts obtained by aerial parts and leaves of Ocimum basilicum L. Correlating with the lack of severe cytotoxicity on skin-derived cell lines and on the in vivo skin measurements, the results obtained in the HET-CAM assay ( xref and xref ) confirms the biocompatibility and good tolerance of both basil hydro-alcoholic extracts on the vascular developing plexus."
sparser
"The investigation has initiated with the evaluation of inhibiting the angiogenesis which is a main activity in metastasis, and it was assessed through Hen’s Egg Test on Chorio Allantoic Membrane (HET-CAM) test; the BSM inhibited the growth of blood vessels in the developing chick embryo."
sparser
"These results revealed that B. hispida peel extracts are suitable for further developing topical products or skin care products with safety similar to that of other extracts that have been reported to have no irritation potential after being tested using the HET-CAM assay [ xref , xref , xref ]"
sparser
"Their in vivo efficacy was evaluated, along with drug retention in porcine and human skin after 30 min of administration, chemical stability at different temperatures, cytotoxicity, histological changes induced via transdermal application, and irritative potential, assessed using the HET-CAM assay. (3) Results: Both formulations reduced inflammation when applied 30 min before perforation compared to the positive control."
sparser
"In case of an unwanted evaporation of a volatile solvent the remaining unresorped solid test substance can be found laying on the inner egg membrane so that the examiner is alerted for a possibly wrong negative outcome of the test.
• Local irritant effects, which can result in an increased mortality due to local effects can be
detected immediately by macroscopic observation in analogy to the established HET-CAM [11]. • The vitality of the embryo can be judged better through the air cell opening than by candling the unopened egg, so that embryos with a reduced vitality can be eliminated from the experiment. • The air cell route is easier in handling than the albumen route."
sparser
"Finally, the safety of the four fermentation broths were evaluated through the red blood cell (RBC) test and hen’s egg test on chorioallantoic membrane (HET-CAM), and the storage stability of the fermentation broths was investigated through changes in the pH value and soluble solid content."
sparser
"The nanomicelle formulations passed the hen's egg-chorioallantoic membrane (HET-CAM) test effectively and significantly improved ALA flow through the cornea and decreased lag time. xref Besides, nanospheres exhibited exceptional levels of physical and chemical stability in order to serve as a therapeutic agent with antioxidant properties and encapsulation of ALA into these particles, offering a promising approach for the prevention and treatment of diseases associated with oxidative stress. xref "
sparser
"The finding that the selected nanoemulsion (containing tributyrin) displays some cytotoxicity against normal cells (although less pronounced compared to cancer cells) prompted us to assess the nanoemulsion potential for causing irritation to the administration site using HET-CAM."
sparser
"Draize’s test, Schimer’s test, HET-CAM (method for evaluating the eye irritation properties) test, permeability and bovine opacity test, cell viability research, and histopathology investigations are only a few of the tests that have been used to look into the security of nanoformulations for ocular administration xref , xref ."
sparser
"In this feasibility study we demonstrated successful imaging and analysis of target-specific radiotracer accumulation in a HET-CAM xenograft model by combined PET and MRI, using the PSMA-specific PET tracer [ 68 Ga]Ga-PSMA-11 and PSMA-positive and PSMA-negative tumor cell lines."
sparser
"With the capability of MRI to provide multiple image contrasts even in the small HET-CAM tumors [ xref ], its potential to provide further important physiological and molecular information (e.g., perfusion, diffusion, and tissue relaxation properties) for a more detailed tumor characterization is of major interest for future studies."
sparser
"The non-irritant behavior of the Soluplus and Pluronic F68 micelle dispersions is in good agreement with previous reports on HET-CAM assay results recorded for 23% w / v Soluplus dispersions [ xref ] and in situ gel formulations prepared with 23% w / v Pluronic F127 and 15% w / v Pluronic F68 [ xref ]."
sparser
"The non-irritant formulation, Mox-mPH NPs (1:150) (proven by HET-CAM test) exhibited intense antimicrobial activity against P. aeruginosa, S. pneumoniae, and S. aureus in vitro analyzed by live-dead cells assay, zone of inhibition studies, and by determining the minimum inhibitory and bactericidal concentrations."
sparser
"The irritation potential of the PRM17 membrane was evaluated by employing an assay that is out of the scope of the ISO 10993-23:2021 standard, dedicated to the evaluation of irritation effects of substances [ xref ], which was the hen’s egg chorioallantoic membrane test (HET-CAM test) [ xref ]."
sparser
"After having carried out toxicity studies as bovine corneal opacity and permeability (BCOP) and Heńs Egg Test - Chorioallantoic Membrane Test (HET-CAM) it was verified that both, the active ingredients and the excipients of the different formulations were not harmful without generating irritation, loss of transparency or corneal permeability alterations."
sparser
"This study explored the in vitro safety, by HET-CAM (hen’s egg test on chorioallantoic membrane), and protective effects of polyphenols (chlorogenic acid, apigenin, kaempferol, and naringenin) against stratum corneum UV-induced lipid peroxidation using an innovative method, the HPLC-TBARS-EVSC (high-performance liquid chromatography–thiobarbituric acid reactive substances– ex vivo stratum corneum), and a stress test using methyl nicotinate and laser Doppler flowmetry to establish in vivo the samples’ topical anti-inflammatory ability."
sparser
"More precisely, the hen egg test—chorioallantoic membrane assay (HET-CAM), which provides evidence on how a sample can affect the vascular functionality of epithelial tissues, can be used to assess the biocompatibility and irritation potential for different types of samples, including vegetal oils [ xref ]."
sparser
"This study employs a combination of in-vitro and in-vivo methods, including the hen’s egg test on chorioallantoic membrane (HET-CAM) for irritation potential, high-performance liquid chromatography–thiobarbituric acid reactive substances– ex vivo stratum corneum (HPLC-TBARS-EVSC) for assessing antioxidant activity, and laser Doppler flowmetry to measure anti-inflammatory effects."
sparser
"Tang et al. evaluated the eye and skin irritation potentials of four plant hair dyes (henna, Chinese gallnuts, sappanwood, and walnut husks) by using several OECD validated in vitro methods, including bovine corneal opacity and permeability (BCOP) assay in combination with histopathological analysis, Hen’s egg test on chick chorioallantoic membrane (HET-CAM), and a test on reconstructed human epidermis models [ xref ]."
sparser
"In addition to safety for ocular applications, evaluation via HET-CAM is also related to the irritant potential in other epitheliums, which can be correlated to the mucous/substrates, such as the vaginal epithelium, demonstrating that its application through different routes of administration are favored [ xref ]."
sparser
"The HET-CAM model, widely used due to its rapid vascular development and methodological simplicity, has proven to be an effective and accessible tool for evaluating compounds with the potential to modulate angiogenesis, acting as either inhibitors or stimulators [ xref , xref ]."
sparser
"The two most potent α-glucosidase inhibitors, aqueous extracts from Geum urbanum L. (avens root) and Rhodiola rosea L. (roseroot) were further examined by in-vitro enzyme and cell culture-based assays as well as by in-ovo (hen’s egg test-chorioallantoic membrane; HET-CAM) and in-vivo models ( D. melanogaster ) to pinpoint potential antidiabetic activities."
sparser
"More specifically, to estimate the biocompatibility and the irritation potential of natural products, the hen egg test chorioallantoic membrane assay (HET-CAM) can be employed, offering characteristics regarding the effect of plant extracts upon the vascular functionality of epithelial tissues [ xref ]."
sparser
"Prior to the experiment, the HET-CAM test was validated using normal saline solution (NSS) as a negative control, whereas sodium lauryl sulfate (SLS) aqueous solution (1% w / v ) was used as a positive control because of its irritating effects on the skin, resulting in increased transepidermal water loss (TEWL) and skin erythema xref ."
sparser
"Since the HET-CAM test has been proposed as a useful in vitro assay for the irritation assessment of cosmetic formulations and ingredients, especially eye irritation xref , it can be concluded that all black D. longan seed extracts are safe for topical application as an active cosmetic ingredient."
sparser
"Regarding the biosafety properties of the mini-implant, current in vitro and in vivo evaluations have shown that the sample extraction medium did not induce impairment of the HGF cell viable population or irritative potential when using the HET-CAM protocol, supporting thus the use of the custom-made mini-implant alloy in further experimental clinical applications."
sparser
"Furthermore, taking into account the direct contact of CHX with both the cutaneous surface and the mucosa of the oral cavity during clinical use, the potential in ovo irritant property of CHX was also determined using the Hen’s Egg-Chorioallantoic Membrane (HET-CAM) test due to its wide application in the assessment of the irritant effects on mucous membranes, and its correlation with the results of dermal irritation tests [ xref , xref ]."
sparser
"HET‐CAM test, a sensitive, rapid, and cost‐effective method using incubated eggs, represents an efficient approach for detection of ocular corrosives and irritants. [ xref ] The HET‐CAM assay demonstrated that the BM/BX SDNs exhibited no signs of irritation upon applied to the CAM, indicating their non‐irritant properties."
sparser
"96: ″ Hen's Egg Test on the Chorioallantoic Membrane (HET‐CAM) provided by EURL ECVAM dataset on alternative methods to animal experimentation (DB‐ALM) guidelines. [ xref ] Accordingly, the time noted in seconds were applied to the predictive model formula to calculated the irritation score (IS) = (301 – H)*5/300 + (301 – L)*7/300 + (301 – C)*9/300."
sparser
"We put in experimental parameters such as the intensity ratio of 1 O 2 and PS phosphorescence at 1270 nm, the triplet decay time of pyropheophorbide-a (pP) in oxygen-free micellar suspensions (around 415 µs), the 1 O 2 kinetics in blood (as determined in a HET-CAM [ xref ] model) and drug concentration both in the tumor and in plasma as well as published parameters for oxygen solubility and diffusion in different types of tissue [ xref , xref ]."
sparser
"In this study, we focused on the evaluation of the cytotoxic profile of ethinylestradiol (EE), levonorgestrel (LNG), and their association (EE + LNG), with and without UVB irradiation, on healthy cell lines (human keratinocytes, fibroblasts, and primary epidermal melanocytes, and mouse epidermis cells) and tumor cell lines (human and murine melanoma) by in vitro (viability, migration and proliferation) and in vivo (HET-CAM) techniques."
sparser
"Although the current form of the HET-CAM test is a valuable prescreen method for predicting the ocular irritation potential of chemicals, and can be used for reducing the number of experimental animals, a number of technical problems must still be addressed before these systems can replace whole animal tests."
sparser
"HET-CAM is a well-accepted ex vivo conjunctival irritation model that produces responses (of hyperemia, hemorrhage, and clotting/coagulation) to test substances similar to those involving the conjunctiva of the eye. xref shows developmental stages of the CAM at 3- and 10-days and different irritant responses to the moderate irritant propylene glycol (PG), a strong irritant (sodium hydroxide 1 M), and F-P3/CS1. xref shows the cumulative numerical irritation scores of the test substances and controls."
sparser
"The final interest of the present study was the in ovo evaluation of SIL-O and SIL-L (100 µM), in terms of vascular toxicity and irritant potential ( xref and xref ), by resorting to the HET-CAM assay which has been already accepted in many European countries as a full replacement method for the evaluation of severe irritants in animal models."
sparser
"The other 13 compounds did not exhibit any detectable mortality
against the chick embryos. xref shows the number of newly formed blood capillaries
in HET-CAM during exposure to 40 μM of the 13 nontoxic compounds
in comparison to the formed ones by DEXA (0.02 μg) as a potent
antiangiogenic agent and using 40% DMSO as a negative control."
sparser
"Compared with other alternative tests, the HET-CAM test has some good points, such as could be carried out for all types of chemical substance (liquid or powder), applied with the similar condition of in vivo test, easy to access for research, not expensive, not need complicated animal room facility, and simple method [ xref , xref ]."
sparser
"The HET-CAM methodology is widely used and goes beyond research laboratories; this test is already used in the industry to identify potential non-irritating or moderately irritating materials during internal screening and safety assessments of formulations and/or raw materials of different physicochemical properties [ xref , xref ]."
sparser
"The HET-CAM assay results presented in xref showed that after 5 min of treatment, the NP formulations did not cause any irritation on the chorioallantoic membrane of fertilized hen’s eggs, thus behaving as the negative control (C−, xref A) and contrary to the positive control (C+) treated in which lysis was observed ( xref B)."
sparser
"The irritation potency of honey aromatic water was evaluated by the hen’s egg test–chorioallantoic membrane (HET-CAM) assay, which was developed as an alternative to the Draize eye irritation test [ xref ] and has been widely used for assessing the irritant potential of cosmetic ingredients designed for facial care, particularly those intended for use in the vicinity of the eye area."
sparser
"The favorable zero-order kinetics that prolonged capsaicin release and the significantly faster transdermal penetration of the NLC attributed to the reduction in skin irritation of the concentrated capsaicin NLCs, as illustrated by the in vitro EpiDerm TM three-dimensional human skin irritation test and hen’s egg test chorioallantoic membrane assay (HET-CAM)."
sparser
"Irritation tests of the chili extract-loaded lipid-based nanoparticle and the gel formulation containing the chili extract-loaded lipid nanocarrier were performed using the hen’s egg test chorioallantoic membrane (HET-CAM) assay and the in vitro skin irritation EpiDerm TM test model, respectively."
sparser
"The absence of vascular reactions after administration of MEL at concentrations further chosen for the in vivo study is crucial to corroborate this choice since hemolysis is a toxic reaction previously reported with MEL ( xref ). xref reported a vascular response in a HET-CAM assay with MEL, but at concentrations 1000x higher than the ones evaluated in this work."
sparser
"Studies comparing the irritation potential of several test substances have demonstrated a good correlation between the HET-CAM and in vivo Draize test, and as such, it is recommended by the Organisation for Economic Co-operation and Development (OECD) as a well-validated alternative to in vivo testing [ xref , xref ]."
sparser
"We evaluated the cell viability, hemolysis potential, and irritation effects of these nanomaterials, showing that both GQDs and GQDs-GSH maintained 100% cell viability in the range of 16.25-260 μg mL -1 , caused minimal RBC hemolysis (<9%), and presented low irritation scores in HET-CAM assays."
sparser
"Consequently, the hen’s egg chorioallantoic membrane (HET-CAM) test, which does not necessitate ethical approval when the age of the hen embryo is less than half of the total incubation period, which is typically less than 10–11 days [ xref ], was employed to assess the irritant potential of these A. mellifera larval extracts."
sparser
"We assayed the effects of venoms from the most widespread venomous African Bitis arietens and closely related species Bitis parviocula using The Hen’s Egg Test—Chorioallantoic membrane test (HET-CAM) and Chicken embryotoxicity screening test (CHEST), acetylcholinesterase (AChE) analysis, cytotoxicity assay performed on cell lines and protein analysis of selected venoms."
sparser
"Based on the similarities of vasoactive effects observed in HET-CAM test, we suggest that the venom from B. arietans and B. parviocula may share certain venom protein families’ responsible for haemotoxicity, such as metalloproteinases and disintegrins, C-type lectins and snaclecs."
sparser
"HET-CAM (Hen’s Egg test-chorioallantoic membrane) method was used to evaluate the irritation potential of the GE-BMopt-IG. xref It is an in vitro test and an alternative to the Draize method for the determination of the irritative index. xref , xref HET-CAM study assigns an irritation score to assess a substance’s propensity to irritate or damage the eye based on its effects on the CAM of a fertilized hen’s egg."
sparser
"The hen egg test–chorioallantoic membrane (HET-CAM) assay has also gained attention in assessing irritability in the field of bioengineering, ophthalmic products as well as cosmeceuticals, as an alternative to the Draize eye rabbit test, according to the 3Rs principles: replacement, reduction, and refinement. [ xref , xref , xref , xref ]."
sparser
"The red blood cell test (RBC) is used to evaluate the irritation of chemicals on eye tissues (Wang et al. xref , xref , xref ) The experimental results showed that all three samples had certain irritability, but there was water in the fermentation broth stock solution which meant that the experimental results would be affected to a certain extent, so the chicken embryo chorioallantoic membrane test (HET-CAM) was added."