IndraLab

Statements


| 231

sparser
"Various toxicity studies, such as Draize and low volume eye tests, and non-ocular models, such as hen’s egg test-chorioallantoic membrane (HET-CAM), have been developed to assess the safety of the formulations."
| PMC

sparser
"Polymyxin B-loaded hydrogels showed good biocompatibility in HET-CAM tests."
| PMC

sparser
"The Hen’s Egg Test-Chorioallantoic Membrane (HET-CAM) assay, corneal hydration study, and histopathology observation showed the non-irritancy of the developed SLN-6, most probably due to the use of physiological compatible lipid and excipients [ xref ]."

sparser
"These results are in agreement with the HET-CAM test confirming the non-irritant potential of DXI loaded polymeric nanoparticles and also the suitability of the HET-CAM test in order to mimic the in vivo irritation phenomena."

sparser
"In this respect, the chick embryo or hen's egg test-chorioallantoic membrane (HET-CAM) model is of special interest, as it is not considered an animal until day 17."

sparser
"HET-CAM study demonstrates that the formulation prepared is non irritant to the highly vascular tissues and hence can be used for the immunological sensitive tissues like inflamed intestine in IBD."

sparser
"We assayed the effects of venoms from the most widespread venomous African Bitis arietens and closely related species Bitis parviocula using The Hen's Egg Test-Chorioallantoic membrane test (HET-CAM) and Chicken embryotoxicity screening test (CHEST), acetylcholinesterase (AChE) analysis, cytotoxicity assay performed on cell lines and protein analysis of selected venoms."

sparser
"The relative safety of the optimized micelles on the ocular tissue was shown with the HET-CAM toxicity test."

sparser
"HET-CAM tests showed that none of the liposomes were irritating (Fig.  xref ), which is suitable for the ocular application."

sparser
"The 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) and hen's egg-chorioallantoic membrane test (HET-CAM) assays confirmed that the complexes and ocular films were nonirritant and safe for ocular administration."

sparser
"This proof-of-principle study shows the general feasibility of the HET-CAM xenograft model for target-specific imaging with PET and MRI."

sparser
"The results of the in vivo anti-inflammatory activity tested by the HET-CAM assay showed that the system had good anti-inflammatory potential compared to free dKTP and identical to free dKTP at one-fifth the dose [130]."

sparser
"After the HET-CAM assay, the FOR711A complex was classified as non-irritant (N.I.) and its vasodilator effect was confirmed through greater evidence of blood vessels after the administration and ending of the observation period of 5 min."

sparser
"HET-CAM and Draize tests revealed that optimized formulation of fluconazole did not result in any irritation and was considered non-irritant and well-tolerated for ocular use."

sparser
"HET-CAM is a qualitative and low sensitivity evaluation method for moderately irritating products that have been entirely used for evaluating eye irritability, alternatively to the use of animal models, which has currently been questioned xref , xref ."

sparser
"The HET-CAM (hens-egg test-chorioallantoic membrane) model can be used in combination with the non-invasive imaging modalities PET and MRI for pre-selection during radiopharmaceutical development to reduce the number of animal experiments required."

sparser
"Irritation Assessment Using the HET-CAM Test."

sparser
"Further, histopathology and HET-CAM studies revealed that the developed gel was nonirritating and safe for ocular administration."

sparser
"Regarding the biosafety properties of the mini-implant, current in vitro and in vivo evaluations have shown that the sample extraction medium did not induce impairment of the HGF cell viable population or irritative potential when using the HET-CAM protocol, supporting thus the use of the custom-made mini-implant alloy in further experimental clinical applications."

sparser
"To evaluate the irritant potential of antifungal combinations, the alternative HET-CAM method (chicken egg test chorioallantoic membrane) was used."

sparser
"Formulation of Chitosan Polymeric Vesicles of Ciprofloxacin for Ocular Delivery: Box-Behnken Optimization, In Vitro Characterization, HET-CAM Irritation, and Antimicrobial Assessment."

sparser
"The results as shown in xref confirmed the reliability of the HET-CAM test on the irritation prediction since severe irritation was detected in a positive control and no irritation was detected in a negative control."

sparser
"32 Here, we used an adapted HET-CAM protocol to test the vasoconstrictive potential of xylometazoline HCl in the presence and absence of iota-carrageenan."

sparser
"The potential irritancy was detected by observing adverse changes which occur in the CAM of the hen’s egg, after exposure to both hydro-alcoholic extracts obtained by aerial parts and leaves of Ocimum basilicum L. Correlating with the lack of severe cytotoxicity on skin-derived cell lines and on the in vivo skin measurements, the results obtained in the HET-CAM assay ( xref and xref ) confirms the biocompatibility and good tolerance of both basil hydro-alcoholic extracts on the vascular developing plexus."

sparser
"The Hen's Egg Test - Chorioallantoic Membrane (HET-CAM) is a valid alternative method used to assess the potential for eye irritation from chemicals."

sparser
"The HET-CAM assay, an internationally validated method, is based on the evaluation of chorioallantoic membrane of embryonated hen’s egg damage in order to assess the potential irritation to the conjunctiva [ xref ]."

sparser
"Finally, TZ release as well as the irritation effect via Hen's Egg test-chorioallantoic membrane (HET-CAM test) of the optimized system was observed in vitro."

sparser
"The Hen’s Egg Test on the Chorioallantoic Membrane (HET-CAM) assay was used to gain an insight into the compatibility of the developed hydrogels with the ocular surface."
| PMC

sparser
"For topical application, a semi-solid hydrogel formulation has also been developed with a gel forming agent (Carbopol® 971); the irritation profile of particles before and after dispersion into hydrogels has been analysed by HET-CAM test."

sparser
"HET-CAM (Hen’s Egg Test Chorio-Allantoic-Membrane) assay is an useful approach to evaluate the cytotoxicity and the irritation of chemicals, considering that the test mimics vascular changes in the chorioallantoic membrane, an analog for ocular conjuntiva [ xref , xref ]."

sparser
"The score of HET-CAM for GTM-CHNPopt sol-gel (NSG5) confirmed that it is safe for ocular administration."

sparser
"In vitro studies such as the chorioallantoic membrane irritation (HET-CAM) assay have been performed demonstrating the threshold for slight irritation (in this case vessel hemorrhage) to be 1 µM [150]."

sparser
"Although the current form of the HET-CAM test is a valuable prescreen method for predicting the ocular irritation potential of chemicals, and can be used for reducing the number of experimental animals, a number of technical problems must still be addressed before these systems can replace whole animal tests."

sparser
"The HET-CAM (hen’s egg chorioallantoic membrane) method was used to evaluate biocompatibility and to explore the potential irritability induced on irrigated mucosal tissues by hydro-alcoholic basil extracts."

sparser
"After having carried out toxicity studies as bovine corneal opacity and permeability (BCOP) and Heńs Egg Test - Chorioallantoic Membrane Test (HET-CAM) it was verified that both, the active ingredients and the excipients of the different formulations were not harmful without generating irritation, loss of transparency or corneal permeability alterations."

sparser
"The HET-CAM test was used to detect possible irritation that may occur to ocular tissue upon application of the optimized systems (Epl-loadedEpl-loaded COSL-MNLCs and Epl-loaded HA-MNLCs), predicting their potential safety."
| PMC

sparser
"To estimate its stability, major long-lived species were quantified across several weeks of storage at −20 • C. Subsequently, its toxic effects against of human malignant cell lines of the peritoneal cavity was investigated in both 2D and 3D models, including the HET-CAM chicken embryo vascularized tumor model."

sparser
"HET-CAM results showed that all combinations can be classified as non-irritating, corroborated by the results of the histopathological evaluation of the pig's ear skin."

sparser
"Although the biocompatibility of SF has long been proved, we showed the angiocompatibility of SF scaffolds for the first time using the HET-CAM assay."

sparser
"Further, CTM-CHNPs were evaluated for physicochemical parameters, in-vitro drug release, ex-vivo permeation, bioadhesive study, corneal hydration, histopathology, HET-CAM, and antibacterial study."

sparser
"These results were employed to develop an algorithm that allows selecting the appropriate HET-CAM protocol for each type of product to be tested."

sparser
"The HET-CAM assay revealed no angioirritative effect of the SF scaffolds."

sparser
"Previous studies using the HET-CAM as a model test system revealed irritating effects of different EOs (Demirci et al. xref ; Moura do Carmo et al. xref ; Reichling et al. xref )."

sparser
"Furthermore, the histopathological assessment and HET-CAM study revealed that the DRZ-CS-PCL-NPs were non-irritant and safe for ocular administration."

sparser
"Irritation Potential Assessment by the Means of HETCAM Test."

sparser
"HET-CAM test was done by adding the formulation directly to the CAM surface and monitoring the vessels visually in terms of irritation reactions."

sparser
"No ocular irritancy was detected by the HET-CAM test."

sparser
"Microencapsulation by spray-drying reduced the volatility, with a prolonged release up to 16 weeks, and decreased the irritation potential of nonencapsulated citronella oil, as evaluated by the nonanimal hen’s egg test-chorioallantoic membrane (HET-CAM) assay [ xref ]."

sparser
"The solution of O. sanctum ethanolic extract (0.1% w/w in 0.9% w/w NSS) induced no irritation in the HET-CAM test as shown in xref ."

sparser
"The HET-CAM test indicated that implants did not induce hemorrhage or coagulation, being non-irritant to the CAM."

sparser
"Safety studies performed using corneal histopathology and Hen Egg Test- Chorio Allantoic Membrane (HET-CAM) assay showed no physical change in cornea or capillary damage, respectively."

sparser
"Photostability assay, bioadhesive property as well as preliminary toxicity parameters (HET-CAM and Artemia salina lethality assays) for free or nanoencapsulated forms were assessed."

sparser
"xref summarizes the conjunctival irritation potential results of all the surfactants, cosurfactants, and oils of interest as determined using the HET-CAM assay."

sparser
"In the present study, 1% w/v SLS, 0.9% w/v NSS, and DI water were used as positive, negative, and vehicle control to ensure the accuracy of the HET-CAM test."

sparser
"For this purpose, the HET-CAM has gained acceptance as an alternative to the rabbit eye irritation test (Draize test) for the assessment of the mucous membrane irritation potential (Barile xref ), and has been extensively used to evaluate the irritation potential of different substances and products (Derouiche and Abdennour xref ; Marquardt et al. xref ; McKenzie et al. xref ; Rajpal Deshmukh et al. xref ; Scheel et al. xref ; Steiling et al. xref )."

sparser
"The actual form of the HET-CAM test is a valuable pre-screen for predicting ocular irritation potential of chemicals, and can be used to reduce the number of experimental animals."

sparser
"The HET-CAM assay has been used for the evaluation of conjunctival responses to test substances."

sparser
"A similar result was replicated in the HET-CAM test where hyperemia was observed in one out of three chorioallantoic membranes, and the irritation mean scores revealed that the optimized formulation was practically non-irritant [ xref ]."

sparser
"Moreover, the potential irritative effect of the release of the metal ions from orthodontic implants has also been investigated in vivo, using the hen’s egg test-chorioallantoic membrane (HET-CAM) assay."

sparser
"The data obtained from applying the HET-CAM assay to evaluate the irritant potential of ASP classify 15 and 30 mM ASP as weak irritant, according to the Luepke score [ xref ]."

sparser
"The hen’s egg test on chorioallantoic membrane (HET-CAM) assay is a simple and versatile alternative to animal testing [ xref ]."

sparser
"Imprinted and non-imprinted hydrogels were prepared with various contents in the functional monomer methacrylic acid (MAA) and characterized in terms of swelling, transmittance, mechanical properties, and ocular compatibility (hen's egg test on chorioallantoic membrane (HET-CAM) assay)."

sparser
"HET-CAM assay showed that concentrations above 0.237 mmol."

sparser
"In sequence, hen's egg test-chorioallantoic membrane (HET-CAM), angiogenic assay, external abnormalities, weight of embryos and oxidative stress markers (TBARS, NPSH, ROS and CAT) were analyzed."

sparser
"As shown in the results of the HET-CAM test, no irritation occurred up to 2 h (mean score 0)."

sparser
"The hen's egg chorioallantoic membrane (HET-CAM) study showed 0 scores (no irritation), and it was further confirmed by corneal hydration and histopathology study."

sparser
"With a design to determine the potential irritating effect of the three volatile oils evaluated in the present study, the HET-CAM method was chosen, which uses a biological model including the chorioallantoic membrane of the chicken egg."
| PMC

sparser
"The HET-CAM test confirmed the compatibility of the hydrogels with vessel functionality since no bleedings, thrombotic events, or vessel destructions were observed."

sparser
"Both basil hydro-alcoholic extracts show a good biocompatibility and tolerance on the vascular developing plexus, as indicated by the HET-CAM assay."

sparser
"Hen’s Egg Test—ChorioAllantoic Membrane (HET-CAM) assay was used to evaluate conjunctival irritation of selected excipients."

sparser
"HET-CAM Test."
| PMC

sparser
"A chitosan nanoparticulate in situ gelling system formulated by Upadhayay et al. also revealed that the 12th h of the HET-CAM test showed visible membrane discoloration but without hemorrhage, suggesting mild irritation [ xref ]."

sparser
"Weakest correlation was found between the data from in vitro HET-CAM and ICE tests."

sparser
"HET-CAM results showed zero scores indicating no bleeding or haemorrhage."

sparser
"HET-CAM studies, histopathological evaluation, and Draize test confirmed the safety of the formulation for ocular use."

sparser
"Comparative evaluation of HET-CAM and ICE methods for objective assessment of ocular irritation caused by selected pesticide products."

sparser
"If the results of HET-CAM assay shows that the substances are not irritant to the eye, they can be instilled in the eye with safety [ xref ]."
| PMC

sparser
"Globule size, polydispersity index, and irritation potential was tested by using Hen’s Egg Test-Chorioallantoic membrane (HET-CAM)."
| PMC

sparser
"Comparison of the different protocols of the Hen's Egg Test-Chorioallantoic Membrane (HET-CAM) by evaluating the eye irritation potential of surfactants."

sparser
"To determine the irritant potential of the three volatile oils (Hr_EO, Cc_EO, and Ob_EO), the HET-CAM method was applied considering that the safety profile is important for further in vivo evaluations."
| PMC

sparser
"HET-CAM has been described as one of the most suitable alternatives to test eye irritation in vitro since it was found to have good correlation with the Draize test [ xref ]."

sparser
"In addition, pharmacokinetic properties in the human organism were predicted through computational simulations and the potential for acute irritation of FOR711A was also investigated in vitro using the hen's egg chorioallantoic membrane (HET-CAM)."

sparser
"Therefore, we studied the angioirritation of the SF scaffolds by the HET-CAM assay."

sparser
"The investigation has initiated with the evaluation of inhibiting the angiogenesis which is a main activity in metastasis, and it was assessed through Hen’s Egg Test on Chorio Allantoic Membrane (HET-CAM) test; the BSM inhibited the growth of blood vessels in the developing chick embryo."

sparser
"HET-CAM assay has been accepted as an alternative model for in vivo tests because of the high sensitivity of the chorioallantoic membrane to show damages in the presence of irritant compounds."

sparser
"Furthermore, Gel C was biocompatible with the Hela cell line (MTT assay) and was classified as a non-severe irritant in the HET-CAM assay (irritation score 4 ± 1)."

sparser
"The HET-CAM assay is an important preliminary test for measuring the ability of a compound to induce toxicity to the mucous membranes."

sparser
"Therefore, the HET-CAM was used as an alternative method to the Draize rabbit eye test for EO irritation testing."

sparser
"The safety of NEs was investigated by the modified Draize test and hen's egg test-chorioallantoic membrane (HET-CAM)."

sparser
"Printed and unprinted hydrogels with different contents of functional methacrylic acid monomer (MAA) were prepared and analysed regarding swelling, transmittance, mechanical properties, and ocular compatibility (hen egg chorioallantoic test (HET-CAM))."
| PMC

sparser
"Anti-Angiogenic Study by HET-CAM Analysis."

sparser
"Four niosomal drug carriers were assessed in another study by Abdelkader et al. for corneal toxicity by the hen’s egg test chorioallantoic membrane (HET-CAM), bovine cornea opacity and permeability (BCOP), and cornea histological test."
| PMC

sparser
"Initially, ketamine toxicity was evaluated by cytotoxicity assay and Hen's egg chorioallantoic membrane (HET-CAM) method."

sparser
"Moreover, the researcher employed the HET-CAM method to evaluate ocular irritation for optimized formulation, as shown in xref [ xref ]."

sparser
"Finally, another alternative PDX platform is provided in the contribution by Winter et al. [ xref ] using the Hen’s egg test–chorioallantoic membrane (HETCAM) PDX model."

sparser
"One of the alternative methods proposed by the Interagency is the hen’s egg test on the chorioallantoic membrane (HET-CAM) [ xref ]."

sparser
"Hen’s egg test-chorioallantoic membrane (HET-CAM) test is a study of the irritation potential of formulations for topical use (skin diseases) or to treat ocular conditions [ xref ]."
| PMC

sparser
"The prepared nanoparticles were devoid of residual solvent and were found to be safe in HET-CAM analysis, RBCs damage analysis and histopathology studies."

sparser
"The Hen’s Egg Test-Chorioallantoic Membrane (HET-CAM) test is based on the detection of vascular damage in the chorioallantoic membrane, which is an analog for ocular conjunctiva [ xref ]."

sparser
"Safety of the developed formulation as a carrier for ophthalmic drug delivery was measured using three different methods: MTT assay for measuring cell viability in which the human retinal pigmentation epithelial cells (RPE) were used, HET-CAM as a borderline method between in vivo and in vitro techniques for investigating the irritation potential of the chosen formulation which was done by adding formulation directly on the CAM surface and visually monitoring the vessels in terms of irritation reactions, and finally the modified Draize test for evaluating tolerability of the selected formulation on eyes."

sparser
"Implants were manufactured by a hot-molding technique, characterized by Fourier Transform Infrared Spectroscopy, X-Ray Diffraction, Differential Scanning Calorimetry, Scanning Electron Microscopy; evaluated in terms of ocular biocompatibility by immunofluorescence, flow cytometry, cell migration, Hen's egg test-chorioallantoic membrane (HET-CAM) irritation test; and investigated in terms of in vitro efficacy against Toxoplasma gondii ."

sparser
"Furthermore, NLC containing O. sanctum ethanolic extract induced no irritation in HET-CAM test."

sparser
"Firstly, used as an alternative to the Draize eye test, the HETCAM protocol is a simple, cheaper and less time-consuming alternative, suitable for assessing irritative and inflammatory potential of various types of chemicals with topical application [ xref , xref ]."

sparser
"Finally, HET-CAM test showed non-irritating response and normal vascularization of the chorioallantoic membrane."

sparser
"The same parameters as for the HET-CAM assay were registered (H, L and S time in seconds), both with and without pre-treatment of test samples."

sparser
"The preliminary assessment of inhibiting the angiogenesis process was successfully carried out through HET-CAM analysis in chick’s embryonated eggs and the DoE inhibited and slowed down the formation of blood vessels in the embryo."

sparser
"A HET-CAM based vascularized intestine tumor model as a screening platform for nano-formulated photosensitizers."

sparser
"A HET-CAM test conducted by Mahboobian et al. revealed that the cumulative score for the acyclovir in situ ocular gel formulation was 0.33."

sparser
"Finally, the HET-CAM test proved the non-irritative potential of NE ASE ."

sparser
"HET-CAM is an inexpensive, short-term in vivo method for predicting the ocular irritant effect of chemicals as an alternative to the classic Draize test in rabbits [ xref , xref ]."

sparser
"As observed by others [ xref ], certain metal elements present in an extraction medium might influence their cytotoxicity, but not necessarily represent an irritant material when evaluated in an in vivo HET-CAM setting."

sparser
"In addition, ocular irritation of the sparfloxacin in situ ophthalmic formulation was assessed by the HET-CAM test."

sparser
"Irritation Study by Hen’s Egg Test on the Chorioallantoic Membrane (HET-CAM)."

sparser
"All suspensions were found to be nonirritating and biocompatible after HET-CAM assay and in vitro cell viability assay in murine fibroblasts."

sparser
"Eye irritation potency of pesticides (fungicides, herbicides, insecticides) was comparatively tested by HET-CAM and ICE method."

sparser
"The non-irritant character of the two compounds revealed by the HET-CAM assay indicated their biocompatibility with mucosal tissues thus enabling their biomedical use ( xref )."
| PMC

sparser
"Additionally, metal alloys were studied and their bioavailability could be established by using the HETCAM assay, with the possibility of analyzing the impact of certain metals released in the tested media [ xref ]."

sparser
"PSMA-specific accumulation of [ 18 F]F-siPSMA-14 was successfully demonstrated in the HET-CAM model, similar to the results obtained by mouse model studies."

sparser
"The optimized formulation exhibited a higher % of growth inhibition on A-431 cells and demonstrated good anti-angiogenic activity in the HET-CAM test."

sparser
"CHNPs did not exhibit any sign of damage to excised goat cornea, which is confirmed by hydration, histopathology, and HET-CAM test."

sparser
"The in vivo test was assessed considering taking into account that each of the formulation compounds was safe and biocompatible, based on the previously performed in vitro HET-CAM test and analysed by other authors [ xref , xref ]."
| PMC

sparser
"After accelerated physical stability and bovine conrneal permeation studies, NE2 was chosen as optimized formulation forantimicrobial efficacy, and hen's egg test-chorioallantoic membrane (HET-CAM) tests."

sparser
"HET-CAM test showed no signs of hemorrhage, coagulation, or lysis for the loaded nanoemulgel same as sodium chloride solution (negative control) where its irritation score was zero compared to 9.87 for the positive irritant group (1%w/v sodium lauryl sulfate)."

sparser
"The formulation proved to be safe for the topical application, as it was non-irritant on both HET-CAM and reconstructed human epidermis (RHE) assays."

sparser
"This study reports the effects of ASE-loaded nanoemulsions (NE ASE ) on the cellular viability, death by necrosis, and migration of immortalized human keratinocytes (HaCaT cell line), as well as the irritant potential through the hen's egg chorioallantoic membrane test (HET-CAM)."

sparser
"The hen's egg test on chorioallantoic membrane (HET-CAM) is one of the most frequently used alternative tests for prediction of ocular irritation of cosmetic products."

sparser
"Eye irritation assay was carried out using the HET-CAM test by comparing innovative timolol maleate in situ ocular gel formulation with normal saline."

sparser
"The nanosystem presented a zeta potential equal to - 30.1 ± 1.8 mV and was stable for at least 90 days when stored at 6°C. The colloidal aqueous dispersion was non-irritating, according to the in vitro HET-CAM test."

sparser
"An ocular irritation study of innovative besifloxacin in situ gel in comparison with sodium hydroxide (NaOH) and normal saline was performed using the HET-CAM test."

sparser
"To the in vitro safety assessment CAM-TBS, HET-CAM, and red blood cell tests were achieved."

sparser
"The HET-CAM is a sensitive, cheap and easily feasible test to investigate potential anti-inflammatory compounds and extracts, and is widely used to assess the irritation potential of test substances."

sparser
"HET-CAM is known to overestimate the results and to be able to accurately identify non-irritant products and it is a great candidate to be part of a Bottom-up test strategy."

sparser
"Thus, the HET-CAM model might also represent a complementary tool for the prediction of LD 50 in vivo."

sparser
"The non-irritation profile of the hybrid nanoparticles before and after dispersion into Carbopol hydrogels was confirmed by HET-CAM test."

sparser
"The cytotoxicity assays and the hen's egg chorioallantoic membrane assay (HET-CAM) confirmed the formulations' safety and non-irritancy."

sparser
"Thus, we evaluated the feasibility of quantitative analysis of target-specific radiotracer accumulation in xenografts using the HET-CAM model and combined positron emission tomography (PET) and magnetic resonance imaging (MRI)."

sparser
"The product that was found to be non-safe in the product use clinical evaluation was also unsuitable by most of the HET-CAM protocols."

sparser
"In this review, we highlight relevant studies in the field and spotlight the applicability of alternative models, including C. elegans , D. rerio , Drosophila , HET-CAM and Lab-on-a-chip."

sparser
"While HETCAM has been predominantly used to investigate vasculature and angiogenesis-targeted interventions, Winter et al. describe the feasibility of high-resolution MRI and PET-imaging methods to evaluate novel radiotracers in this CDX-based HETCAM model."

sparser
"Thus, the use of the HET-CAM model is suitable for the pre-selection of new radiopharmaceuticals and potentially reduces animal testing in line with the 3Rs principles of animal welfare."

sparser
"The present form of the HET-CAM test can be proposed as a pre-screening method for the determination of eye irritative potential, therefore the number of test animals can be reduced and/or experimental animals can be replaced."

sparser
"Hydrogels exhibited properties for topical administration and did not induce irritation effects on the HET-CAM."

sparser
"All the products that were classified as non-irritant by any HET-CAM protocols were also safe in the product use clinical study."

sparser
"The Hen’s Egg Test-Chorioallantoic Membrane (HET-CAM) assay, corneal hydration study, and histopathology observation showed the non-irritancy of the developed SLN-6, most probably due to the use of physiological compatible lipid and excipients [45]."

sparser
"The HET-CAM assay revealed the biocompatibility of the tested compounds with mucosal tissues, thus making them safe for the purpose of therapeutic applications."
| PMC

sparser
"The Hen's egg test on chorioallantoic membrane (HET-CAM) model provides the generation of vascularized tumor spheroids and therefore, is an ideal test platform which can be used as an intermediate step between in vitro analysis and preclinical evaluation in vivo."

sparser
"The HET-CAM assay confirmed that the formulations were not irritants as negative controls (IS = 0) ( xref ); the IS for the positive control was around 17."

sparser
"They were also non-irritant according to HET-CAM tests."

sparser
"The results of the in vivo anti-inflammatory activity tested by the HET-CAM assay showed that the system had good anti-inflammatory potential compared to free dKTP and identical to free dKTP at one-fifth the dose [ xref ]."

sparser
"Secondly, the biocompatibility was tested on HET-CAM, and rabbit models under modified Draize eye protocol revealed that the in situ gel with T. arjuna was non-irritant for ocular application."

sparser
"The HET-CAM test results were similar to the test results of Gupta et al. This indicated that the chitosan/gellan gum based in situ gel was a non-irritant to mild irritant."

sparser
"Irritations at risk of vascular damage, such as bleeding, lysis, and coagulation, can be evaluated by HET-CAM test, which is optimal for the study of compound accuracy and safety for ocular application. xref , xref "

sparser
"Furthermore, HET-CAM and modified Draize test confirmed that F1 and F2 were safe for ocular administration."

sparser
"The present form of the HET-CAM test can be proposed as a pre-screen method of eye irritation tests, therefore the number of test animals can be reduced."

sparser
"Comparison of Quantification of Target-Specific Accumulation of [18F]F-siPSMA-14 in the HET-CAM Model and in Mice Using PET/MRI."

sparser
"The F3 formulation (CyA: 60.08 ± 2.85 μg) did not induce conjunctival irritation when HET-CAM assay was performed and was hence considered suitable for further study in a rabbit eye."

sparser
"Statistical analysis of agreement between classification revealed significant correlation between results of in vivo and in HET-CAM assays (76%)."

sparser
"Conjunctival Irritation of Surfactants, Cosurfactants, and Oils Determined by the HET-CAM Assay."

sparser
"The toxicity of the compounds was investigated by means of HET-CAM assay ( xref ), which is a reliable Ex vivo test used to mimic in vivo inflammatory reactions and recognized as a predictive model for the irritant effect on conjunctival tissues [ xref ]."
| PMC

sparser
"Good ocular tolerance of the formulation was observed both in an HET-CAM test, where no sign of vascular response was observed, and in a Draize test, performed on New Zealand white rabbits where no sign of inflammation or excessive lacrimation was observed [ xref , xref ]."

sparser
"The results obtained from the HET-CAM investigation didn't show any sign of vessel injury on the CAM surface for prepared formulation."

sparser
"Hen's egg test on the chorioallantoic membrane (HET-CAM) HET-CAM assay has been accepted as an alternative model for in vivo tests because of the high sensitivity of the chorioallantoic membrane to show damages in the presence of irritant compounds."

sparser
"HET-CAM was carried out to test ocular irritation and revealed no signs of membrane discoloration or hemorrhage."

sparser
"In addition, the four ceramic materials were examined in vivo using the HETCAM assay in order to establish their irritative potential upon mucosal highly vascularized tissues."

sparser
"Ocular Irritancy Test (HET-CAM)."

sparser
"Besides that, these nanoparticles showed a high occlusion factor and non-irritant potential in HET-CAM assay."

sparser
"In this regard, the authors performed in vitro (characterization of the hydrogels, drug release and cell toxicity) and ex vivo (transcorneal permeation and Hen´s Egg Chorioallantoic membrane (HET-CAM)) assays."

sparser
"The nanocomposites were characterized by morphological analysis, FTIR, Raman Spectroscopy, thermal analysis and cytotoxicity assays (MTT and HET-CAM)."

sparser
"We developed a HET-CAM based intestine tumor model to investigate the accumulation and efficacy of nano-formulated photosensitizers."

sparser
"Therefore, the HET-CAM test was well validated in the present study."

sparser
"The formulations successfully passed the hen's egg-chorioallantoic membrane test (HET-CAM) test."

sparser
"Antibiogram (disk diffusion), chromatographic analysis (UPLC), and toxicity assay (HET-CAM) were also performed."

sparser
"Among them, cell and tissue culture models, the hen’s egg test on the chorioallantoic membrane (HET-CAM) as well as the Caenorhabditis elegans ( C. elegans ) model have gained considerable attention as alternative screening platforms to evaluate the toxicity of bioactive compounds in various context (Haselgrubler et al. xref ; Hunt xref ; Piersma xref ; Scheel et al. xref )."

sparser
"HET-CAM test indicated no irritation, and HL% revealed no damage to the tissue, so the optimum NE is well tolerated by the eye."

sparser
"The techniques used to examine the safety profile of those polymers include the HET-CAM test, gamma scintigraphy study, handheld infra-red (IR) assay, histopathology study, and in vivo eye irritation assay."

sparser
"HET-CAM testing was performed, wherein formulated micellar in situ gel showed faintly visible membrane discoloration in one tested egg at 480 min and another egg at 720 min."

sparser
"Within this regard, the HET-CAM test method showed potential to refine and reduce animals used in irritation testing (Barile xref )."

sparser
"HET-CAM."

sparser
"By now, several alternative parameters like lysis, hyperemia and vasoconstriction have been evaluated using adapted HET-CAM protocols."

sparser
"In vitro ocular tolerance was studied by HET-CAM test."
| PMC

sparser
"Olopatadine-loaded CL successfully passed the HET-CAM eye irritation test and showed good mast cell compatibility."
| PMC

sparser
"Multi-Modal PET and MR Imaging in the Hen's Egg Test-Chorioallantoic Membrane (HET-CAM) Model for Initial"

sparser
"The results of the reconstructed human epidermis model, human corneal epithelium model and consumer research showed that no irritation was found in any test products; however, HET-CAM tests showed positive results."

sparser
"Toxicity of PnPP-19 using HET-CAM Assay."

sparser
"The irritant effects can be observed in the CAM of fertile eggs following the exposure to a substance in the HET-CAM test which is analogous to the Draize rabbit-eye test [ xref ]."

sparser
"HET-CAM Test."

sparser
"The purpose of this study was to compare the different protocols of the HET-CAM, the French and German protocols, by evaluating the eye irritation potential of surfactants."

sparser
"The HET-CAM test is a useful tool for studying in vivo the potential conjunctival irritation, while the ICE test can be used to study corneal irritant effects in detail."

sparser
"The non-irritant formulation, Mox-mPH NPs (1:150) (proven by HET-CAM test) exhibited intense antimicrobial activity against P. aeruginosa, S. pneumoniae, and S. aureus in vitro analyzed by live-dead cells assay, zone of inhibition studies, and by determining the minimum inhibitory and bactericidal concentrations."

sparser
"All hydrogels showed physical properties adequate for CLs and successfully passed the hen’s egg-chorioallantoic membrane (HET-CAM) test."
| PMC

sparser
"NTX-loaded hydrogels were highly cytocompatible with human mesenchymal stem cells (hMSC) and passed the HET-CAM test."

sparser
"There are different HET-CAM protocols widely accepted, but there is no information about which of the protocols better correlates with the results obtained in product use clinical study under the conditions of use."

sparser
"Results: The results correlate the composition and topography of the implant surface with biological experimental evaluations related to directly affected cells (gingival fibroblasts) and toxicological results on blood vessels (hen’s egg test-chorioallantoic membrane (HET-CAM) assay)."

sparser
"The designed mini-implant alloy, exposed to the perfused chorioallantoic membrane, showed no sign of irritation and no alteration of vascular parameters in the HET-CAM assay ( xref )."

sparser
"In vitro alternative methods consisting of the keratinocyte cytotoxicity assay, red blood cell (RBC) test, and hen's egg test-chorioallantoic membrane (HET-CAM) were performed to elucidate the mechanism of preservative-induced irritation responses."

sparser
"Ocular tolerance test (HET-CAM)."

sparser
"Hen’s egg-chorioallantoic membrane (HET-CAM) test was applied showing that the positive controls (NaOH 1 M) resulted in severe hemorrhage, which increased over five minutes grading this solution as a severe irritant."

sparser
"The analysis of HET-CAM results revealed that both NI and AI were biocompatible, which suggested their applicability as ingredients for facial treatment."

sparser
"The in-situ nanoemulgel ocular irritation was studied by HET-CAM test."

sparser
"Vasoactive effects were visualized using the Hen's Egg Test - Chorioallantoic membrane (HET-CAM) test."

sparser
"A Hen’s Egg Test-Chorioallantoic membrane (HET-CAM) test revealed that the gel was slightly irritative [ xref ]."

sparser
"HET-CAM assessment is a well-established in-vitro parameter for determination of ocular tolerability of the test sample, xref and scores are depicted in xref ."

sparser
"Since the HET-CAM test has been suggested as a valid method reliably applied in a research on potentially toxic substances [ xref ], it could be concluded that O. sanctum ethanolic extract both in the solution and nanodelivery system had no toxicity and was safe for topical applications."

sparser
"Moreover, in the Hen’s egg test-chorioallantoic membrane (HET-CAM) ( xref c–e), the in ovo ocular nontoxicity of all the samples was determined, which demonstrated the biocompatibility for their use as facial cosmetics or pharmaceutical ingredients."

sparser
"In addition, potential adverse effects of the hydrogels were determined using the "Hen's egg test on chorioallantoic membrane" (HET-CAM) test and a mouse model."

sparser
"Finally, the formulations presented no irritant effect in the HET-CAM assay."

sparser
"HET-CAM Assay."
| PMC

sparser
"The hen’s egg chorioallantoic membrane test (HET-CAM) showed that blood vessels were neither expanded nor shrunk after treatments with myricetin, blank-LNP, and M-LNP (Fig.  xref c,d)."

sparser
"All hydrogels showed physical properties adequate for CLs and successfully passed the hen's egg-chorioallantoic membrane (HET-CAM) test."

sparser
"In vitro ocular irritation (HET-CAM) test: Cont."

sparser
"Comparison between HET-CAM protocols and a product use clinical study for eye irritation evaluation of personal care products including cosmetics according to their surfactant composition."

sparser
"Achyrocline satureioides (Lam.) DC (Asteraceae) Extract-Loaded Nanoemulsions as a Promising Topical Wound Healing Delivery System: In Vitro Assessments in Human Keratinocytes (HaCaT) and HET-CAM Irritant Potential."

sparser
"The hen's egg test-chorioallantoic membrane (HET-CAM), as a borderline test between in vivo and in vitro techniques, was chosen for investigating the irritation potential of the formulation."

sparser
"Effect of Compounds 6 and 7 on the Normal and Tumor Angiogenesis Process by CAM Assay and Irritation Potential Determination Using the HET-CAM Assay."
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sparser
"Herein, we studied ex vivo permeation, penetration, anti-fungal activity, and Hen's Egg Test-Chorioallantoic Membrane (HET-CAM) toxicity tests in order to assess ocular targeting of PSC micelles, which were optimized in our previous study."

sparser
"The results correlate the composition and topography of the implant surface with biological experimental evaluations related to directly affected cells (gingival fibroblasts) and toxicological results on blood vessels (hen's egg test-chorioallantoic membrane (HET-CAM) assay)."

sparser
"Hen's egg test on the chorioallantoic membrane (HET-CAM), which is similar to the structure of the human cornea and has a clear and complete vascular system, is based on the characteristics of the chorioallantoic membrane (CAM) in mid-term SPF egg embryos."

sparser
"We have also carried out the hen's egg-chlorioallantoic membrane test (HET-CAM) to evaluate the ocular irritancy of the different antioxidant solutions."

sparser
"As far as we know, these two volatile oils ( H. rhamoides and C. citratus ) have not been tested for the irritating effect by employing the HET-CAM method."
| PMC

sparser
"The microemulsion was investigated for the irritation properties in comparison with nonencapsulated OEO using HET-CAM (hen egg test-chorioallantoic membrane) assay."

sparser
"NC-FA and NC-B were able to interact with mucin, which is an indicative of mucoadhesive properties and the association of FA with nanocapsules showed decreased irritation by HET-CAM method."

sparser
"HET-CAM assay was used in order to assess the toxicity of compounds 6 and 7 ( xref ); the procedure is a low-cost, unique model in biomedical research [ xref ] that allows the testing of the irritation potency of chemical compounds while simultaneously avoiding in vivo experiments [ xref ]."
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sparser
"Liposomes elaborated with phosphatidylcholine (LP PC) or phosphatidylcholine and spermine (LP PC: SPM) were stable under storage at 6 ºC for 30 days, showed mucoadhesive characteristics, and were non-irritant, according to HET-CAM tests."

sparser
"Annatto extract did not demonstrate cytotoxicity in the HET-CAM assay or MTT assay for fibroblast culture."

sparser
"Thus, the mean score at the end of 720 min of the HET-CAM test was 0.67, which suggested it was a mild irritant."

sparser
"As the eye is more sensitive than healthy skin, and are commonly in contact with cosmetic and other UV blockers, the HET-CAM test has been run to measure the potential risk of irritation of Parsol-SLN-Si before and after their dispersion into hydrogels."

sparser
"An in vivo hen's egg-chorioallantoic membrane (HET-CAM) angiogenic study was carried out for 21 compounds 6a , b , d , f , h , i , k-o , t , and 6x to check their mortality and toxicity."

sparser
"At this concentration, CW was safe for topical applications since it induced no irritation signs on the CAM in HET-CAM test."

sparser
"The biocompatibility of irrigant solutions, including 8.25% sodium hypochlorite (NaOCl), was analyzed in samples (n = 25) of chorioallantoic membranes (CAM) from embryos of the Gallus gallus, using the hen's egg test on chorioallantoic membrane (HET-CAM)."

sparser
"Effect of Compounds 4 and 6 on Normal and Tumor Angiogenesis Process by CAM Assay and Irritation Potential Determination Using the HET-CAM Assay."
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sparser
"The favorable zero-order kinetics that prolonged capsaicin release and the significantly faster transdermal penetration of the NLC attributed to the reduction in skin irritation of the concentrated capsaicin NLCs, as illustrated by the in vitro EpiDerm TM three-dimensional human skin irritation test and hen's egg test chorioallantoic membrane assay (HET-CAM)."