IndraLab

Statements


| 428

sparser
"We decided to employ the model of chicken chorioallantoic membrane (CAM) and The Hen’s Egg Test—Chorioallantoic membrane test (HET-CAM) for visualization and evaluation of the effects of B. parviocula and B. arietans venom on vasculature."

sparser
"An in vitro HET-CAM test was carried out and OII was calculated."

sparser
"Polymyxin B-loaded hydrogels showed good biocompatibility in HET-CAM tests."
| PMC

sparser
"The histopathology and HET-CAM results revealed that the prepared formulation is safe for ocular use."

sparser
"The irritation of the BN-BSog was evaluated by using an in vitro HET-CAM test, which is an alternative to the skin irritation test."

sparser
"The HET-CAM test revealed that Soluplus and Pluronic F68 micelle dispersions behaved as the negative control (0.9% NaCl) without inducing bleeding, lysis or coagulation at the time of the study ( t H , t L , and t C > 301 s; IS = 0), which means that they can be considered non-irritants ( xref )."

sparser
"All formulations successfully passed the HET-CAM assay but showed Pluronic®-casein dose-dependent toxicity in the zebrafish embryo model."

sparser
"To maintain objectivity in the evaluation process of potential eye irritation, the HET-CAM test requires the use of a reference substance."

sparser
"The developed semi-solid sunscreen formulation containing the benzophenone-3-loaded NCs exhibited appropriate SPF values, without irritation potential in HET-CAM test."

sparser
"HET-CAM study demonstrates that the formulation prepared is non irritant to the highly vascular tissues and hence can be used for the immunological sensitive tissues like inflamed intestine in IBD."

sparser
"The test by the HET-CAM confirmed no skin irritation of the complex extract."

sparser
"The 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) and hen's egg-chorioallantoic membrane test (HET-CAM) assays confirmed that the complexes and ocular films were nonirritant and safe for ocular administration."

sparser
"One of them is the chicken egg chorioallantoic membrane (CAM) developed to detect a chemical test called the Hen’s Egg Test Chorioallantoic Membrane (HET-CAM) assay [ xref ]."

sparser
"The results of the in vivo anti-inflammatory activity tested by the HET-CAM assay showed that the system had good anti-inflammatory potential compared to free dKTP and identical to free dKTP at one-fifth the dose [130]."

sparser
"After the HET-CAM assay, the FOR711A complex was classified as non-irritant (N.I.) and its vasodilator effect was confirmed through greater evidence of blood vessels after the administration and ending of the observation period of 5 min."

sparser
"HET-CAM and Draize tests revealed that optimized formulation of fluconazole did not result in any irritation and was considered non-irritant and well-tolerated for ocular use."

sparser
"HET-CAM tests suggest that the produced materials do not lead to irritation."
| PMC

sparser
"However, the HET-CAM test is not suitable for red-pigmented samples."

sparser
"First screening of the ocular compatibility of the inserts with ocular tissues was assessed by HET-CAM assays."

sparser
"The HET-CAM (hens-egg test-chorioallantoic membrane) model can be used in combination with the non-invasive imaging modalities PET and MRI for pre-selection during radiopharmaceutical development to reduce the number of animal experiments required."

sparser
"Further, histopathology and HET-CAM studies revealed that the developed gel was nonirritating and safe for ocular administration."

sparser
"Regarding the biosafety properties of the mini-implant, current in vitro and in vivo evaluations have shown that the sample extraction medium did not induce impairment of the HGF cell viable population or irritative potential when using the HET-CAM protocol, supporting thus the use of the custom-made mini-implant alloy in further experimental clinical applications."

sparser
"If successfully established, combined PET-MR imaging using the HET-CAM xenograft model would allow for a substantial reduction of animal experiments in line with the 3Rs principles."

sparser
"To evaluate the irritant potential of antifungal combinations, the alternative HET-CAM method (chicken egg test chorioallantoic membrane) was used."

sparser
"Formulation of Chitosan Polymeric Vesicles of Ciprofloxacin for Ocular Delivery: Box-Behnken Optimization, In Vitro Characterization, HET-CAM Irritation, and Antimicrobial Assessment."

sparser
"The potential irritancy was detected by observing adverse changes which occur in the CAM of the hen’s egg, after exposure to both hydro-alcoholic extracts obtained by aerial parts and leaves of Ocimum basilicum L. Correlating with the lack of severe cytotoxicity on skin-derived cell lines and on the in vivo skin measurements, the results obtained in the HET-CAM assay ( xref and xref ) confirms the biocompatibility and good tolerance of both basil hydro-alcoholic extracts on the vascular developing plexus."

sparser
"The Hen's Egg Test - Chorioallantoic Membrane (HET-CAM) is a valid alternative method used to assess the potential for eye irritation from chemicals."

sparser
"The HET-CAM assay, an internationally validated method, is based on the evaluation of chorioallantoic membrane of embryonated hen’s egg damage in order to assess the potential irritation to the conjunctiva [ xref ]."

sparser
"Finally, TZ release as well as the irritation effect via Hen's Egg test-chorioallantoic membrane (HET-CAM test) of the optimized system was observed in vitro."

sparser
"The HET-CAM test revealed that GSH solutions (10 mg/mL, 5 mg/mL) and the solution of 5 mg/mL GSH with 3 mg/mL of Vit C, did not produce hemorrhages, lysis, or coagulation ( xref )."

sparser
"The Hen’s Egg Test on the Chorioallantoic Membrane (HET-CAM) assay was used to gain an insight into the compatibility of the developed hydrogels with the ocular surface."
| PMC

sparser
"Despite these methodological challenges, the HET-CAM model still appears very attractive from the cost and administrative burden point of view."

sparser
"Ketorolac Tromethamine Loaded Nano-Spray Dried Nanoparticles: Preparation, Characterization, Cell Viability, COL1A1 Gene Simulation and Determination of Anti-inflammatory Activity by In Vivo HET-CAM Assay."

sparser
"Finally, the tolerance of the formulations on the ocular tissues was evaluated by the HET-CAM technique, as well as through the histological analysis of the cornea and sclera and the cornea transparency."

sparser
"The HET-CAM test has advantages over other tests due to its speed, simplicity, convenience, and relatively low cost [ xref ]."

sparser
"In vitro studies such as the chorioallantoic membrane irritation (HET-CAM) assay have been performed demonstrating the threshold for slight irritation (in this case vessel hemorrhage) to be 1 µM [150]."

sparser
"The HET-CAM test was used to detect possible irritation that may occur to ocular tissue upon application of the optimized systems (Epl-loadedEpl-loaded COSL-MNLCs and Epl-loaded HA-MNLCs), predicting their potential safety."

sparser
"To estimate its stability, major long-lived species were quantified across several weeks of storage at −20 • C. Subsequently, its toxic effects against of human malignant cell lines of the peritoneal cavity was investigated in both 2D and 3D models, including the HET-CAM chicken embryo vascularized tumor model."

sparser
"Although the biocompatibility of SF has long been proved, we showed the angiocompatibility of SF scaffolds for the first time using the HET-CAM assay."

sparser
"The HET-CAM assay revealed no angioirritative effect of the SF scaffolds."

sparser
"Previous studies using the HET-CAM as a model test system revealed irritating effects of different EOs (Demirci et al. xref ; Moura do Carmo et al. xref ; Reichling et al. xref )."

sparser
"Furthermore, the histopathological assessment and HET-CAM study revealed that the DRZ-CS-PCL-NPs were non-irritant and safe for ocular administration."

sparser
"Irritation Potential Assessment by the Means of HETCAM Test."

sparser
"No ocular irritancy was detected by the HET-CAM test."

sparser
"The formulation was tested for irritation potential using the HET-CAM test score, which revealed it to be nonirritant, similar to the negative control."

sparser
"HET-CAM was performed in order to study the ocular irritation of the prepared formulations which is dependent on the active as well as the excipients used at their respective concentrations."

sparser
"Microencapsulation by spray-drying reduced the volatility, with a prolonged release up to 16 weeks, and decreased the irritation potential of nonencapsulated citronella oil, as evaluated by the nonanimal hen’s egg test-chorioallantoic membrane (HET-CAM) assay [ xref ]."

sparser
"The HET-CAM test indicated that implants did not induce hemorrhage or coagulation, being non-irritant to the CAM."

sparser
"It is of interest that similar to our results in the HET-CAM model, a minor ligand uptake was also observed for PC-3 tumors in mice."

sparser
"The two most potent α-glucosidase inhibitors, aqueous extracts from Geum urbanum L. (avens root) and Rhodiola rosea L. (roseroot) were further examined by in-vitro enzyme and cell culture-based assays as well as by in-ovo (hen’s egg test-chorioallantoic membrane; HET-CAM) and in-vivo models ( D. melanogaster ) to pinpoint potential antidiabetic activities."

sparser
"The formulations successfully passed the hen’s egg-chorioallantoic membrane test (HET-CAM) test."

sparser
"Safety studies performed using corneal histopathology and Hen Egg Test- Chorio Allantoic Membrane (HET-CAM) assay showed no physical change in cornea or capillary damage, respectively."

sparser
"Methodological and Logistical Aspects of the HET-CAM Xenograft Model."

sparser
"Ex vivo ocular irritation and tolerance studies were performed using the HET-CAM; 8-day-old, incubated hen’s eggs were used for the same."

sparser
"In the present study, 1% w/v SLS, 0.9% w/v NSS, and DI water were used as positive, negative, and vehicle control to ensure the accuracy of the HET-CAM test."

sparser
"For this purpose, the HET-CAM has gained acceptance as an alternative to the rabbit eye irritation test (Draize test) for the assessment of the mucous membrane irritation potential (Barile xref ), and has been extensively used to evaluate the irritation potential of different substances and products (Derouiche and Abdennour xref ; Marquardt et al. xref ; McKenzie et al. xref ; Rajpal Deshmukh et al. xref ; Scheel et al. xref ; Steiling et al. xref )."

sparser
"The HET-CAM assay has been used for the evaluation of conjunctival responses to test substances."

sparser
"Moreover, the potential irritative effect of the release of the metal ions from orthodontic implants has also been investigated in vivo, using the hen’s egg test-chorioallantoic membrane (HET-CAM) assay."

sparser
"The hen’s egg test on chorioallantoic membrane (HET-CAM) assay is a simple and versatile alternative to animal testing [ xref ]."

sparser
"The HET-CAM test was used for a first screening of ocular tolerance of the developed hydrogels."

sparser
"Micro images of a blood vessel in the HET-CAM test are shown in xref ."

sparser
"With a design to determine the potential irritating effect of the three volatile oils evaluated in the present study, the HET-CAM method was chosen, which uses a biological model including the chorioallantoic membrane of the chicken egg."
| PMC

sparser
"The HET-CAM test confirmed the compatibility of the hydrogels with vessel functionality since no bleedings, thrombotic events, or vessel destructions were observed."

sparser
"The HET-CAM assay was used to show the irritation potential of these essential oils because it is rapid and inexpensive."

sparser
"Both basil hydro-alcoholic extracts show a good biocompatibility and tolerance on the vascular developing plexus, as indicated by the HET-CAM assay."

sparser
"Hen’s Egg Test—ChorioAllantoic Membrane (HET-CAM) assay was used to evaluate conjunctival irritation of selected excipients."

sparser
"HET-CAM Test."
| PMC

sparser
"Weakest correlation was found between the data from in vitro HET-CAM and ICE tests."

sparser
"The cytotoxicity of chitin and chitosan were verified using the HET-CAM test."

sparser
"HET-CAM results showed zero scores indicating no bleeding or haemorrhage."

sparser
"To test the impact of the selected extracts on the blood glucose level in a living organism, the recently established HET-CAM model was used ( xref ; xref )."

sparser
"Comparative evaluation of HET-CAM and ICE methods for objective assessment of ocular irritation caused by selected pesticide products."

sparser
"If the results of HET-CAM assay shows that the substances are not irritant to the eye, they can be instilled in the eye with safety [ xref ]."
| PMC

sparser
"Globule size, polydispersity index, and irritation potential was tested by using Hen’s Egg Test-Chorioallantoic membrane (HET-CAM)."
| PMC

sparser
"Comparison of the different protocols of the Hen's Egg Test-Chorioallantoic Membrane (HET-CAM) by evaluating the eye irritation potential of surfactants."

sparser
"Consistent with this finding, the selected nanocarrier (without ceramide) caused no irritation at the site of administration, as evidenced by the low irritation score in HET-CAM models and lack of histological changes in the mammary tissue."

sparser
"In addition, pharmacokinetic properties in the human organism were predicted through computational simulations and the potential for acute irritation of FOR711A was also investigated in vitro using the hen's egg chorioallantoic membrane (HET-CAM)."

sparser
"HET-CAM has been described as one of the most suitable alternatives to test eye irritation in vitro since it was found to have good correlation with the Draize test [ xref ]."

sparser
"Thus, we evaluated the feasibility of quantitative analysis of target-specific radiotracer accumulation in xenografts using the HET-CAM model and combined positron emission tomography (PET) and magnetic resonance imaging (MRI)."

sparser
"The polymeric NCs were then formulated as a semi-solid sunscreen, for which the sun protection factor (SPF), stability, viscosity, and compatibility by hen’s egg-chorioallantoic membrane test (HET-CAM) were determined [ xref ]."

sparser
"In the present study, we aim to repurpose the HET-CAM and SkinEthic™ models to further classify in vivo nonirritant baby cleansing formulations into mild and less mild categories."

sparser
"The investigation has initiated with the evaluation of inhibiting the angiogenesis which is a main activity in metastasis, and it was assessed through Hen’s Egg Test on Chorio Allantoic Membrane (HET-CAM) test; the BSM inhibited the growth of blood vessels in the developing chick embryo."

sparser
"Furthermore, Gel C was biocompatible with the Hela cell line (MTT assay) and was classified as a non-severe irritant in the HET-CAM assay (irritation score 4 ± 1)."

sparser
"The safety of NEs was investigated by the modified Draize test and hen's egg test-chorioallantoic membrane (HET-CAM)."

sparser
"Printed and unprinted hydrogels with different contents of functional methacrylic acid monomer (MAA) were prepared and analysed regarding swelling, transmittance, mechanical properties, and ocular compatibility (hen egg chorioallantoic test (HET-CAM))."
| PMC

sparser
"Anti-Angiogenic Study by HET-CAM Analysis."

sparser
"Moreover, the researcher employed the HET-CAM method to evaluate ocular irritation for optimized formulation, as shown in xref [ xref ]."

sparser
"As a relatively simple, quick and inexpensive model, HET-CAM has already been used for studies on, e.g., angiogenesis [ xref ], cancer progression [ xref ], pharmacology [ xref ], and radiotherapy [ xref ]."

sparser
"Finally, another alternative PDX platform is provided in the contribution by Winter et al. [ xref ] using the Hen’s egg test–chorioallantoic membrane (HETCAM) PDX model."

sparser
"The discussed data comprise in vivo measurements in tumor-laden HET-CAM and mice."

sparser
"One of the alternative methods proposed by the Interagency is the hen’s egg test on the chorioallantoic membrane (HET-CAM) [ xref ]."

sparser
"Hen’s egg test-chorioallantoic membrane (HET-CAM) test is a study of the irritation potential of formulations for topical use (skin diseases) or to treat ocular conditions [ xref ]."
| PMC

sparser
"The prepared nanoparticles were devoid of residual solvent and were found to be safe in HET-CAM analysis, RBCs damage analysis and histopathology studies."

sparser
"The Hen’s Egg Test-Chorioallantoic Membrane (HET-CAM) test is based on the detection of vascular damage in the chorioallantoic membrane, which is an analog for ocular conjunctiva [ xref ]."

sparser
"Further, CIP-BLO-opt-IG3 does not display any toxicity as confirmed by corneal hydration (76.15%), histology, and the HET-CAM test (zero scores)."

sparser
"Safety of the developed formulation as a carrier for ophthalmic drug delivery was measured using three different methods: MTT assay for measuring cell viability in which the human retinal pigmentation epithelial cells (RPE) were used, HET-CAM as a borderline method between in vivo and in vitro techniques for investigating the irritation potential of the chosen formulation which was done by adding formulation directly on the CAM surface and visually monitoring the vessels in terms of irritation reactions, and finally the modified Draize test for evaluating tolerability of the selected formulation on eyes."

sparser
"The potential irritant effect of the vascular plexus of Methotrexate and Cetuximab was analyzed using the HET-CAM method."

sparser
"Implants were manufactured by a hot-molding technique, characterized by Fourier Transform Infrared Spectroscopy, X-Ray Diffraction, Differential Scanning Calorimetry, Scanning Electron Microscopy; evaluated in terms of ocular biocompatibility by immunofluorescence, flow cytometry, cell migration, Hen's egg test-chorioallantoic membrane (HET-CAM) irritation test; and investigated in terms of in vitro efficacy against Toxoplasma gondii ."

sparser
"Furthermore, NLC containing O. sanctum ethanolic extract induced no irritation in HET-CAM test."

sparser
"Firstly, used as an alternative to the Draize eye test, the HETCAM protocol is a simple, cheaper and less time-consuming alternative, suitable for assessing irritative and inflammatory potential of various types of chemicals with topical application [ xref , xref ]."

sparser
"Finally, HET-CAM test showed non-irritating response and normal vascularization of the chorioallantoic membrane."

sparser
"The same parameters as for the HET-CAM assay were registered (H, L and S time in seconds), both with and without pre-treatment of test samples."

sparser
"The preliminary assessment of inhibiting the angiogenesis process was successfully carried out through HET-CAM analysis in chick’s embryonated eggs and the DoE inhibited and slowed down the formation of blood vessels in the embryo."

sparser
"In this study, we focused on the evaluation of the cytotoxic profile of ethinylestradiol (EE), levonorgestrel (LNG), and their association (EE + LNG), with and without UVB irradiation, on healthy cell lines (human keratinocytes, fibroblasts, and primary epidermal melanocytes, and mouse epidermis cells) and tumor cell lines (human and murine melanoma) by in vitro (viability, migration and proliferation) and in vivo (HET-CAM) techniques."

sparser
"The irritation assessment was also carried out with Hen's Egg Test-Chorioallantoic Membrane model (HET-CAM), and in vivo evaluation of the IOP lowering effect was also performed on rats."

sparser
"Irritation Study by Hen’s Egg Test on the Chorioallantoic Membrane (HET-CAM)."

sparser
"All suspensions were found to be nonirritating and biocompatible after HET-CAM assay and in vitro cell viability assay in murine fibroblasts."

sparser
"Eye irritation potency of pesticides (fungicides, herbicides, insecticides) was comparatively tested by HET-CAM and ICE method."

sparser
"Additionally, metal alloys were studied and their bioavailability could be established by using the HETCAM assay, with the possibility of analyzing the impact of certain metals released in the tested media [ xref ]."

sparser
"Consequently, the PET-based activity concentration was underestimated and for a PV correction the system-specific properties of the respective PET system must therefore be considered in future HET-CAM studies."

sparser
"PSMA-specific accumulation of [ 18 F]F-siPSMA-14 was successfully demonstrated in the HET-CAM model, similar to the results obtained by mouse model studies."

sparser
"We have successfully synthesized low- and medium-viscosity PDMS materials using a low-grade D4 monomer and characterized the physical properties, including the in vitro toxicity test by the HET-CAM test method."

sparser
"A first screening of ocular compatibility was carried out placing the formulation in contact with the hen´s egg chorioallantoic membrane (HET-CAM)."

sparser
"The formulation proved to be safe for the topical application, as it was non-irritant on both HET-CAM and reconstructed human epidermis (RHE) assays."

sparser
"This study reports the effects of ASE-loaded nanoemulsions (NE ASE ) on the cellular viability, death by necrosis, and migration of immortalized human keratinocytes (HaCaT cell line), as well as the irritant potential through the hen's egg chorioallantoic membrane test (HET-CAM)."

sparser
"The hen's egg test on chorioallantoic membrane (HET-CAM) is one of the most frequently used alternative tests for prediction of ocular irritation of cosmetic products."

sparser
"Eye irritation assay was carried out using the HET-CAM test by comparing innovative timolol maleate in situ ocular gel formulation with normal saline."

sparser
"Recently, several studies have been published about tumor imaging in the HET-CAM model, focusing on MRI compounds [ xref ] or therapeutic effects after specific and non-specific accumulation [ xref , xref ], demonstrating the vast potential of these method."

sparser
"All emulsions showed weak irritant potential in HET-CAM assay."

sparser
"The HET-CAM is a sensitive, cheap and easily feasible test to investigate potential anti-inflammatory compounds and extracts, and is widely used to assess the irritation potential of test substances."

sparser
"HET-CAM is known to overestimate the results and to be able to accurately identify non-irritant products and it is a great candidate to be part of a Bottom-up test strategy."

sparser
"Thus, the HET-CAM model might also represent a complementary tool for the prediction of LD 50 in vivo."

sparser
"The non-irritation profile of the hybrid nanoparticles before and after dispersion into Carbopol hydrogels was confirmed by HET-CAM test."

sparser
"The cytotoxicity assays and the hen's egg chorioallantoic membrane assay (HET-CAM) confirmed the formulations' safety and non-irritancy."

sparser
"While HETCAM has been predominantly used to investigate vasculature and angiogenesis-targeted interventions, Winter et al. describe the feasibility of high-resolution MRI and PET-imaging methods to evaluate novel radiotracers in this CDX-based HETCAM model."

sparser
"Thus, the use of the HET-CAM model is suitable for the pre-selection of new radiopharmaceuticals and potentially reduces animal testing in line with the 3Rs principles of animal welfare."

sparser
"The present form of the HET-CAM test can be proposed as a pre-screening method for the determination of eye irritative potential, therefore the number of test animals can be reduced and/or experimental animals can be replaced."

sparser
"Based on HET-CAM test evaluation, negative control (NaCl 0.9%) was non-irritant, positive control (1% SDS) was a strong irritant, and all PDMS samples get zero (0) score that indicated as non-irritant substances."

sparser
"The Hen’s Egg Test-Chorioallantoic Membrane (HET-CAM) assay, corneal hydration study, and histopathology observation showed the non-irritancy of the developed SLN-6, most probably due to the use of physiological compatible lipid and excipients [45]."

sparser
"The HET-CAM assay revealed the biocompatibility of the tested compounds with mucosal tissues, thus making them safe for the purpose of therapeutic applications."
| PMC

sparser
"The HET-CAM represents an optimal pre-screening alternative method before animal testing, which is also useful as safety assessment for cutaneous applications [ xref , xref , xref ]."

sparser
"Irritation was evaluated by hen’s egg test-chorioallantoic membrane assay (HET-CAM)."

sparser
"Results from HET-CAM assay indicated that F2 was well tolerated."

sparser
"The HET-CAM assay confirmed that the formulations were not irritants as negative controls (IS = 0) ( xref ); the IS for the positive control was around 17."

sparser
"Formulation was found to be non-irritant in HET-CAM assay."

sparser
"Thus, in contrast to animal studies, the HET-CAM model does not rise legal authorization issues if the embryos are sacrificed accordingly."

sparser
"They were also non-irritant according to HET-CAM tests."

sparser
"For this purpose, a modified hens egg test (termed Gluc-HET; [ xref , xref ]), which was developed in our lab on the basis of the well-established hens egg test-chorioallantoic membrane (HET-CAM) assay [ xref ], has turned out to be a promising strategy."

sparser
"Secondly, the biocompatibility was tested on HET-CAM, and rabbit models under modified Draize eye protocol revealed that the in situ gel with T. arjuna was non-irritant for ocular application."

sparser
"The HET-CAM test results were similar to the test results of Gupta et al. This indicated that the chitosan/gellan gum based in situ gel was a non-irritant to mild irritant."

sparser
"Based on these promising data, further validation studies of the HET-CAM model compared to standard mice xenograft experiments are justified and warranted."

sparser
"Furthermore, HET-CAM and modified Draize test confirmed that F1 and F2 were safe for ocular administration."

sparser
"Conclusions : Summarizing, Cet presents a safer toxicological profile, compared to Met, based on the results obtained from both in vitro (cell viability, wound healing, RT-PCR assays), and in ovo (HET-CAM assay) techniques."

sparser
"Comparison of Quantification of Target-Specific Accumulation of [18F]F-siPSMA-14 in the HET-CAM Model and in Mice Using PET/MRI."

sparser
"The F3 formulation (CyA: 60.08 ± 2.85 μg) did not induce conjunctival irritation when HET-CAM assay was performed and was hence considered suitable for further study in a rabbit eye."

sparser
"Statistical analysis of agreement between classification revealed significant correlation between results of in vivo and in HET-CAM assays (76%)."

sparser
"Irritation results evaluated by HET-CAM showed that the irritation of polyglyceryl-10 monocaprylate (moderate irritant) was comparable with olive oil PEG-8 esters and much lower than that of PEG-6 caprylic/capric glycerides (severe irritant)."

sparser
"Conjunctival Irritation of Surfactants, Cosurfactants, and Oils Determined by the HET-CAM Assay."

sparser
"This facilitates the transfer from an established mouse model to the HET-CAM model for at least initial evaluation."

sparser
"Through the application of HET-CAM, the biocompatibility and potential anti-irritant, as well as protective effects on the vascular plexus were also assessed."

sparser
"Supramolecular gels showed good cytocompatibility against SAOS-2 cells and in the HET-CAM test [ xref ]."

sparser
"The unloaded nanocarrier was considered safe, as indicated by a score <0.1 in HET-CAM models, by the high survival rates of Galleria mellonella larvae exposed to concentrations ≤500 mg/mL, and absence of histological changes when intraductally administered in rats."

sparser
"In addition, the four ceramic materials were examined in vivo using the HETCAM assay in order to establish their irritative potential upon mucosal highly vascularized tissues."

sparser
"Importantly, all copolymer concentrations tested successfully passed the HET-CAM assay."

sparser
"We conclude that the HET-CAM and SkinEthic™ models are promising in vitro alternatives for screening mild formulations."

sparser
"Ocular Irritancy Test (HET-CAM)."

sparser
"Cytotoxicity Test using Chorioallantoic Membrane (HET-CAM Test)."

sparser
"Besides that, these nanoparticles showed a high occlusion factor and non-irritant potential in HET-CAM assay."

sparser
"We also successfully performed dynamic scanning in the HET-CAM model for analysis of time–activity curves (TACs), however no quantitative analysis was performed because of the missing perfusion phase as measurements could not be started earlier than 10 min."

sparser
"The nanocomposites were characterized by morphological analysis, FTIR, Raman Spectroscopy, thermal analysis and cytotoxicity assays (MTT and HET-CAM)."

sparser
"We developed a HET-CAM based intestine tumor model to investigate the accumulation and efficacy of nano-formulated photosensitizers."

sparser
"Furthermore, according to the HET-CAM and reconstructed human epidermis assays, the nanoformulation was well tolerated."

sparser
"To ensure safety to healthy areas, we assessed the cytotoxicity of the unloaded nanoemulsion against non-tumor cells, and its irritation potential in HET-CAM models and in vivo ."

sparser
"Antibiogram (disk diffusion), chromatographic analysis (UPLC), and toxicity assay (HET-CAM) were also performed."

sparser
"After a screening of ocular compatibility using the HET-CAM test, ex vivo drug permeability through the cornea and sclera from the three selected animal species was investigated."

sparser
"HET-CAM test indicated no irritation, and HL% revealed no damage to the tissue, so the optimum NE is well tolerated by the eye."

sparser
"Comparing the absolute values and the ratios of tracer accumulation in PSMA + and PSMA − tumors of all ligands listed, the results of our HET-CAM experiments are very comparable and within the same order of magnitude, which further corroborates our data."

sparser
"Within this regard, the HET-CAM test method showed potential to refine and reduce animals used in irritation testing (Barile xref )."

sparser
"HET-CAM testing was performed, wherein formulated micellar in situ gel showed faintly visible membrane discoloration in one tested egg at 480 min and another egg at 720 min."

sparser
"HET-CAM Test."

sparser
"HET-CAM."

sparser
"From the hen’s egg-chorioallantoic membrane test (HET-CAM) test, the non-irritation profile of the developed formulations could also be confirmed."

sparser
"In vitro drug release studies were performed in simulated nasal electrolyte solution (SNES) maintaining constant pH of 5.5 at 37°C. Irritation by CNF was evaluated using hen’s egg test chorioallantoic membrane (HET-CAM) assay."

sparser
"Toxicity of PnPP-19 using HET-CAM Assay."

sparser
"HET-CAM Test."

sparser
"In the mouse model tumor growth could easily be checked by palpation, whereas it is more challenging in the HET-CAM model."

sparser
"Chorioallantoic membrane (HET-CAM) tests were done to study potential irritation of the skin."
| PMC

sparser
"The HET-CAM test is a useful tool for studying in vivo the potential conjunctival irritation, while the ICE test can be used to study corneal irritant effects in detail."

sparser
"The non-irritant formulation, Mox-mPH NPs (1:150) (proven by HET-CAM test) exhibited intense antimicrobial activity against P. aeruginosa, S. pneumoniae, and S. aureus in vitro analyzed by live-dead cells assay, zone of inhibition studies, and by determining the minimum inhibitory and bactericidal concentrations."

sparser
"There are different HET-CAM protocols widely accepted, but there is no information about which of the protocols better correlates with the results obtained in product use clinical study under the conditions of use."

sparser
"Results: The results correlate the composition and topography of the implant surface with biological experimental evaluations related to directly affected cells (gingival fibroblasts) and toxicological results on blood vessels (hen’s egg test-chorioallantoic membrane (HET-CAM) assay)."

sparser
"Previous studies have reported a good correlation between in vitro tests with HET-CAM and in vivo tests with the Draize method."

sparser
"Several studies have been conducted to evaluate the feasibility of using HET-CAM as a complete replacement for the in vivo rabbit ocular test."

sparser
"To evaluate the cytotoxicity and biocompatibility of chitin and chitosan, Hen's Egg Tests (HETs) were performed on the chorioallantoic membrane (HET-CAM) as per the methodology described by Steiling et al. [ xref ]."

sparser
"Irritant Potential Assessment of Ethinylestradiol and Levonorgestrel by the Means of a HET-CAM Assay."

sparser
"HET-CAM is a reliable screening assay for the non-irritating potential of colloidal systems such as nanoparticles."

sparser
"In vitro alternative methods consisting of the keratinocyte cytotoxicity assay, red blood cell (RBC) test, and hen's egg test-chorioallantoic membrane (HET-CAM) were performed to elucidate the mechanism of preservative-induced irritation responses."

sparser
"Ocular tolerance test (HET-CAM)."

sparser
"The analysis of HET-CAM results revealed that both NI and AI were biocompatible, which suggested their applicability as ingredients for facial treatment."

sparser
"The in-situ nanoemulgel ocular irritation was studied by HET-CAM test."

sparser
"The results of the in vitro toxicity test using the HET-CAM method showed that PDMS with low and medium viscosity was a non-irritant substance indicating that all samples were non-toxic."

sparser
"Since the HET-CAM test has been suggested as a valid method reliably applied in a research on potentially toxic substances [ xref ], it could be concluded that O. sanctum ethanolic extract both in the solution and nanodelivery system had no toxicity and was safe for topical applications."

sparser
"However, since the in vitro results are not able to reproduce all of the in vivo conditions, Draize eye irritation of free Lico-A, Lico-A PLGA NPs, Lico-A PLGA-PEG-Tet1 NPs, and Lico-A PLGA-PEG-B6 NPs was carried out to confirm the HET-CAM classification [ xref ]."

sparser
"Before extending the analysis on human volunteers, the safety of Micah was assessed by the usual tests: genotoxicity (Ames test), ocular irritation (HET-CAM), phototoxicity (MTT ex vivo and in vivo on 22 volunteers), and sensitization (human repeated insult patch test on 50 volunteers)."

sparser
"In this respect, the hen’s egg test-chorioallantoic membrane (HET-CAM) model is a promising alternative for animal experiments, as in many countries, chick embryos are not considered live animals before embryo development day (EDD) 17 or hatching."

sparser
"Overall, HET-CAM results indicated that the designed inserts can be considered as non-irritant."

sparser
"Moreover, in the Hen’s egg test-chorioallantoic membrane (HET-CAM) ( xref c–e), the in ovo ocular nontoxicity of all the samples was determined, which demonstrated the biocompatibility for their use as facial cosmetics or pharmaceutical ingredients."

sparser
"It also exhibited good tolerability and irritation established by the hydration study, histopathology and HET-CAM test."

sparser
"Finally, the formulations presented no irritant effect in the HET-CAM assay."

sparser
"HET-CAM Assay."
| PMC

sparser
"The HET-CAM test is widely used to evaluate the potential eye irritation of raw materials [ xref , xref , xref ]."

sparser
"HET-CAM Test."

sparser
"In vitro ocular irritation (HET-CAM) test: Cont."

sparser
"Comparison between HET-CAM protocols and a product use clinical study for eye irritation evaluation of personal care products including cosmetics according to their surfactant composition."

sparser
"The hen's egg test-chorioallantoic membrane (HET-CAM), as a borderline test between in vivo and in vitro techniques, was chosen for investigating the irritation potential of the formulation."

sparser
"Effect of Compounds 6 and 7 on the Normal and Tumor Angiogenesis Process by CAM Assay and Irritation Potential Determination Using the HET-CAM Assay."
| PMC

sparser
"The irritant potential (IR) of the formulations was evaluated by the hen's egg chorioallantoic membrane test (HET-CAM), with no irritation phenomena observed."

sparser
"Irritation Properties of Chili Extract Determined by the HET-CAM Assay."

sparser
"The ocular safety of the formulations was determined by BCOP and HET-CAM."

sparser
"Hen's egg test on the chorioallantoic membrane (HET-CAM), which is similar to the structure of the human cornea and has a clear and complete vascular system, is based on the characteristics of the chorioallantoic membrane (CAM) in mid-term SPF egg embryos."

sparser
"Previous studies showed that HET-CAM and in vivo eye irritation tests have a good correlation [ xref ]."

sparser
"The HET-CAM assay is a predictive model for in vitro irritation by observing the vascular damage and calculating the irritation score (IS) [ xref ]."

sparser
"The NCs were shown to be well tolerated, and no signs of irritancy or alterations of the eye properties were detected by the in vitro HET-CAM test and in vivo Draize test."

sparser
"Samples were non-irritant through the HET-CAM test."

sparser
"HET-CAM assay was used in order to assess the toxicity of compounds 6 and 7 ( xref ); the procedure is a low-cost, unique model in biomedical research [ xref ] that allows the testing of the irritation potency of chemical compounds while simultaneously avoiding in vivo experiments [ xref ]."
| PMC

sparser
"Annatto extract did not demonstrate cytotoxicity in the HET-CAM assay or MTT assay for fibroblast culture."

sparser
"Thus, the mean score at the end of 720 min of the HET-CAM test was 0.67, which suggested it was a mild irritant."

sparser
"As the eye is more sensitive than healthy skin, and are commonly in contact with cosmetic and other UV blockers, the HET-CAM test has been run to measure the potential risk of irritation of Parsol-SLN-Si before and after their dispersion into hydrogels."

sparser
"In this feasibility study we demonstrated successful imaging and analysis of target-specific radiotracer accumulation in a HET-CAM xenograft model by combined PET and MRI, using the PSMA-specific PET tracer [ 68 Ga]Ga-PSMA-11 and PSMA-positive and PSMA-negative tumor cell lines."

sparser
"The finding that the selected nanoemulsion (containing tributyrin) displays some cytotoxicity against normal cells (although less pronounced compared to cancer cells) prompted us to assess the nanoemulsion potential for causing irritation to the administration site using HET-CAM."

sparser
"More samples were evaluated by HET-CAM, and their irritation scores are summarized in xref ."

sparser
"Effect of Compounds 4 and 6 on Normal and Tumor Angiogenesis Process by CAM Assay and Irritation Potential Determination Using the HET-CAM Assay."
| PMC

sparser
"The hen’s egg test on chorioallantoic membrane (HET-CAM) has been developed as a toxicological method to determine ocular irritation potential ( xref )."

sparser
"According to the HET-CAM scores, strong anti-inflammatory activity was obtained by the F9-HP coded gel at a dose of 20 µg.embryo-1 (p<0.05 compared with SDS)."

sparser
"Results showed all formulations were non-irritant according to the HET-CAM test."

sparser
"Various toxicity studies, such as Draize and low volume eye tests, and non-ocular models, such as hen’s egg test-chorioallantoic membrane (HET-CAM), have been developed to assess the safety of the formulations."
| PMC

sparser
"The results of HET-CAM assay, shown in xref indicated no irritation potential of PC-C32-PC and Me 2 PE-C32-Me 2 PE, respectively."

sparser
"In addition, the HET-CAM test also needs an experienced investigator [ xref ]."

sparser
"Wohlrab et al. [ xref ] conducted a study on the biosafety of Methotrexate using the HET-CAM method, the results suggesting that when it was used in concentrations of 0.25% and 0.5%, it does not cause significant changes in the vascular plexus."

sparser
"For the HET-CAM, the medians of mean irritation scores (IS) were 3.0 for mild formulations (with 0 ≤ mean IS ≤4.5), and 5.0 for less mild formulations (with mean IS values all equaled 5), respectively."

sparser
"The Hen’s Egg Test-Chorioallantoic Membrane (HET-CAM) assay, corneal hydration study, and histopathology observation showed the non-irritancy of the developed SLN-6, most probably due to the use of physiological compatible lipid and excipients [ xref ]."

sparser
"These results are in agreement with the HET-CAM test confirming the non-irritant potential of DXI loaded polymeric nanoparticles and also the suitability of the HET-CAM test in order to mimic the in vivo irritation phenomena."

sparser
"HET-CAM."

sparser
"The HET-CAM test was used to evaluate the irritation potential of these oils as topical products, showing that these oils can be used as auxiliary medication in cases of cutaneous leishmaniasis, with less side effects and lower costs."

sparser
"The relative safety of the optimized micelles on the ocular tissue was shown with the HET-CAM toxicity test."

sparser
"Additionally, the HET-CAM method led to the observation of a strong irritating effect associated with nicotine."

sparser
"HET-CAM tests showed that none of the liposomes were irritating (Fig.  xref ), which is suitable for the ocular application."

sparser
"We have also carried out the hen’s egg-chlorioallantoic membrane test (HET-CAM) to evaluate the ocular irritancy of the different antioxidant solutions."

sparser
"HET-CAM is a qualitative and low sensitivity evaluation method for moderately irritating products that have been entirely used for evaluating eye irritability, alternatively to the use of animal models, which has currently been questioned xref , xref ."

sparser
"The Hen’s Egg Test Chorioallantoic Membrane (HET-CAM) assay has been used to assess the irritation potential of substances [ xref ] and plant extracts, and has proven useful in screening natural products [ xref ]."

sparser
"Irritation Assessment Using the HET-CAM Test."

sparser
"HET-CAM."

sparser
"The results as shown in xref confirmed the reliability of the HET-CAM test on the irritation prediction since severe irritation was detected in a positive control and no irritation was detected in a negative control."

sparser
"32 Here, we used an adapted HET-CAM protocol to test the vasoconstrictive potential of xylometazoline HCl in the presence and absence of iota-carrageenan."

sparser
"Our feasibility study suggests that the HET-CAM xenograft model using PET and MR imaging is promising for the initial assessment of specific radiotracer accumulation under in vivo conditions and the results of our study are in line with the reported data in mice xenografts."

sparser
"For topical application, a semi-solid hydrogel formulation has also been developed with a gel forming agent (Carbopol® 971); the irritation profile of particles before and after dispersion into hydrogels has been analysed by HET-CAM test."

sparser
"Imprinted and non-imprinted hydrogels were prepared with various contents in the functional monomer methacrylic acid (MAA) and characterized in terms of swelling, transmittance, mechanical properties, and ocular compatibility (hen’s egg test on chorioallantoic membrane (HET-CAM) assay)."

sparser
"HET-CAM (Hen’s Egg Test Chorio-Allantoic-Membrane) assay is an useful approach to evaluate the cytotoxicity and the irritation of chemicals, considering that the test mimics vascular changes in the chorioallantoic membrane, an analog for ocular conjuntiva [ xref , xref ]."

sparser
"The score of HET-CAM for GTM-CHNPopt sol-gel (NSG5) confirmed that it is safe for ocular administration."

sparser
"Although the current form of the HET-CAM test is a valuable prescreen method for predicting the ocular irritation potential of chemicals, and can be used for reducing the number of experimental animals, a number of technical problems must still be addressed before these systems can replace whole animal tests."

sparser
"The HET-CAM (hen’s egg chorioallantoic membrane) method was used to evaluate biocompatibility and to explore the potential irritability induced on irrigated mucosal tissues by hydro-alcoholic basil extracts."

sparser
"After having carried out toxicity studies as bovine corneal opacity and permeability (BCOP) and Heńs Egg Test - Chorioallantoic Membrane Test (HET-CAM) it was verified that both, the active ingredients and the excipients of the different formulations were not harmful without generating irritation, loss of transparency or corneal permeability alterations."

sparser
"HET-CAM results showed that all combinations can be classified as non-irritating, corroborated by the results of the histopathological evaluation of the pig's ear skin."

sparser
"The HET-CAM test results showed that all samples did not show signs of irritation indicating that samples were non-toxic."

sparser
"Together with the fact that BZP3 is aggressive to skin and eye, we have carried out the ocular irritation study of our BZP3-loaded NCs in a HET-CAM assay."

sparser
"Further, CTM-CHNPs were evaluated for physicochemical parameters, in-vitro drug release, ex-vivo permeation, bioadhesive study, corneal hydration, histopathology, HET-CAM, and antibacterial study."

sparser
"The HET-CAM findings verified the formulation's eligibility for biological applications."

sparser
"The HET-CAM test has a good correlation with in vivo ocular irritation."

sparser
"These results were employed to develop an algorithm that allows selecting the appropriate HET-CAM protocol for each type of product to be tested."

sparser
"The HET-CAM study showed that BN-BSog was found to be nonirritant as the score was found within the limit."

sparser
"The HET-CAM assay classified the hydrogels as nonirritating."

sparser
"In this respect, the chick embryo or hen’s egg test–chorioallantoic membrane (HET-CAM) model is of special interest, as it is not considered an animal until day 17."

sparser
"HET-CAM test was done by adding the formulation directly to the CAM surface and monitoring the vessels visually in terms of irritation reactions."

sparser
"Unfortunately, blocking experiments could not yet be done, as double injection in the HET-CAM model is quite challenging and single injection with samples separated by air bubbles might kill the embryo."

sparser
"The solution of O. sanctum ethanolic extract (0.1% w/w in 0.9% w/w NSS) induced no irritation in the HET-CAM test as shown in xref ."

sparser
"The hen’s egg test on the chorioallantoic membrane (HET-CAM) (an alternative to the in vivo Draize rabbit eye test) was carried out to assess the potential irritancy of GSH and Vit C solutions."

sparser
"Photostability assay, bioadhesive property as well as preliminary toxicity parameters (HET-CAM and Artemia salina lethality assays) for free or nanoencapsulated forms were assessed."

sparser
"The HET-CAM model allows the easy introduction of various tumor models, not only based on human cell lines but also on rodent cell lines or patient-derived material [ xref , xref , xref , xref , xref , xref , xref , xref ]."

sparser
"xref summarizes the conjunctival irritation potential results of all the surfactants, cosurfactants, and oils of interest as determined using the HET-CAM assay."

sparser
"This optimized formulation was proven with the HET-CAM assay and EpiDerm TM model, which showed that the chili extract-loaded NLC minimized the irritation compared to the chili extract formula."

sparser
"The actual form of the HET-CAM test is a valuable pre-screen for predicting ocular irritation potential of chemicals, and can be used to reduce the number of experimental animals."

sparser
"A similar result was replicated in the HET-CAM test where hyperemia was observed in one out of three chorioallantoic membranes, and the irritation mean scores revealed that the optimized formulation was practically non-irritant [ xref ]."

sparser
"Based on this finding, the micro bioreactors (MBR) design, first made of PDMS and that also passed with cell tests on the HET-CAM, was finally printed in PEGDA, and applied in vivo."

sparser
"The data obtained from applying the HET-CAM assay to evaluate the irritant potential of ASP classify 15 and 30 mM ASP as weak irritant, according to the Luepke score [ xref ]."

sparser
"HET-CAM of Bolalipid Aerogels."

sparser
"Recently, NE loaded P. emarginatus essential oil demonstrated high kinetic stability and was non-irritating, estimated by HET-CAM [40] ."

sparser
"HET-CAM assay showed that concentrations above 0.237 mmol."

sparser
"Capsaicin was encapsulated within the solid matrix and gradually released from the nanoparticles, thus leading to less irritation in the HET-CAM assay."

sparser
"In sequence, hen's egg test-chorioallantoic membrane (HET-CAM), angiogenic assay, external abnormalities, weight of embryos and oxidative stress markers (TBARS, NPSH, ROS and CAT) were analyzed."

sparser
"As shown in the results of the HET-CAM test, no irritation occurred up to 2 h (mean score 0)."

sparser
"The HET-CAM method has become widely embraced as a substituent of the in vivo Draize test, being extensively applied to evaluate the irritation potential of various substances ( xref )."

sparser
"HET-CAM is an easy-to-use and highly sensitive in vitro method for predicting the ocular irritation effect of cosmetic products."

sparser
"The hen's egg chorioallantoic membrane (HET-CAM) study showed 0 scores (no irritation), and it was further confirmed by corneal hydration and histopathology study."

sparser
"Gordon Winter et al. introduced the use of hen’s egg test chorioallantoic membrane model (HET-CAM) in MRI for in vivo testing of novel target-specific radioligands [ xref ]."

sparser
"Therefore, these results agree with those obtained by the HET-CAM test confirming its suitability for the evaluation of the ocular tolerance of pharmaceutical products [ xref ]."

sparser
"A chitosan nanoparticulate in situ gelling system formulated by Upadhayay et al. also revealed that the 12th h of the HET-CAM test showed visible membrane discoloration but without hemorrhage, suggesting mild irritation [ xref ]."

sparser
"Sparfloxacin-loaded PLGA nanoparticles of 180-190 nm particle size were tested using the HET-CAM assay and earned a 0 score over an 8 h period for the nanosuspension ( xref )."

sparser
"Additionally, the hen's egg test on the chorioallantoic membrane (HET-CAM) method was used to assess the biocompatibility and irritant potential of the chorioallantoic membrane."

sparser
"HET-CAM studies, histopathological evaluation, and Draize test confirmed the safety of the formulation for ocular use."

sparser
"An alternative to the Draize test is the chorioallantoic membrane (HET-CAM test), a sensitive and not expensive method, that uses fertilized hen eggs."

sparser
"Thirty out of 35 formulations were predicted consistently between the HET-CAM and SkinEthic™ model."

sparser
"Compared with other alternative tests, the HET-CAM test has some good points, such as could be carried out for all types of chemical substance (liquid or powder), applied with the similar condition of in vivo test, easy to access for research, not expensive, not need complicated animal room facility, and simple method [ xref , xref ]."

sparser
"The in vitro ocular irritation based on modified hen’s egg chorioallantoic membrane (HET-CAM) assay was adopted to investigate the conjunctival irritation of selected in situ gels (Abdelkader et al., xref )."

sparser
"Table 1: Parameters of texture analyzer used to measure the mucoadhesion of the prepared formulations with goat cornea Table 2: Summary of the batches of Posaconazole nanosuspension as suggested by the DoE software Table 3: HET-CAM potential irritancy score and inference deduced based on the time required for the endpoints to develop Table 4: Zone of inhibition of different groups in the antifungal assay Table 5: Characterization and stability results of the Posaconazole nanosuspension Fig. 1: Forced degradation chromatograms of Posaconazole."

sparser
"To determine the irritant potential of the three volatile oils (Hr_EO, Cc_EO, and Ob_EO), the HET-CAM method was applied considering that the safety profile is important for further in vivo evaluations."
| PMC

sparser
"We also reported for the first time the in vitro toxicity test of these PDMS samples using the HET-CAM test method to obtain information safety and toxicity level of all PDMS samples."

sparser
"Therefore, we studied the angioirritation of the SF scaffolds by the HET-CAM assay."

sparser
"HET-CAM assay has been accepted as an alternative model for in vivo tests because of the high sensitivity of the chorioallantoic membrane to show damages in the presence of irritant compounds."

sparser
"The HET-CAM assay is an important preliminary test for measuring the ability of a compound to induce toxicity to the mucous membranes."

sparser
"Therefore, the HET-CAM was used as an alternative method to the Draize rabbit eye test for EO irritation testing."

sparser
"Four niosomal drug carriers were assessed in another study by Abdelkader et al. for corneal toxicity by the hen’s egg test chorioallantoic membrane (HET-CAM), bovine cornea opacity and permeability (BCOP), and cornea histological test."
| PMC

sparser
"The irritation profile of the extracts was evaluated by the hen's egg test-chorioallantoic membrane (HET-CAM) test."

sparser
"Initially, ketamine toxicity was evaluated by cytotoxicity assay and Hen's egg chorioallantoic membrane (HET-CAM) method."

sparser
"Additionally, the results of the HET-CAM assay confirmed that the DFB-LPHNPs were not toxic for ophthalmic administration."

sparser
"HET-CAM (irritation) study was performed for EM-NLCs-opt-IG4, normal saline (0.9% NaCl, negative control) and positive control 0.1N sodium hydroxide) using a fertilized hen egg’s ( xref )."

sparser
"The developed formulations exhibited a controlled release profile, dillapiole delivery up to the dermis, the layer of interest for anti-inflammatory potential, and low irritant potential in the chorioallantoic membrane (HET-CAM)."

sparser
"The irritation study of the negative control (0.9%w / vNaCl), positive control (1%w / vsodium lauryl sulphate), and prepared BN-BSog was evaluated using the HET-CAM test."

sparser
"With the obtained cell viability, gene expression, and HET-CAM results, it has the hope of a successful nano-topical formulation, especially in both wound healing and anti-inflammatory treatment."

sparser
"HET-CAM test confirmed that the prepared formulations were non-irritant."

sparser
"These results are consistent with similar studies which evaluated the irritancy of antibiotics or hormones using the HET-CAM test [ xref , xref ]."

sparser
"Although in vitro techniques are not yet able to fully replace in vivo techniques, for some assays, however, such as the Draize test, there is already a correlation with in vitro methods, such as the HET-CAM assay."

sparser
"HET-CAM was considered important in assessing the ocular irritation potential and, despite classifying almost all surfactants as &quot;severe irritants&quot;, it could discriminate moderate and slight irritant SLES concentrations as well as Cocoamidopropyl Betaine as a severe irritant, when the coagulation score was taken into consideration."

sparser
"HET-CAM study is an in vitro method used to determine the irritation potential of the selected formulation (EM-NLCs-opt-IG4) and the result was compared with normal saline (negative control) and 0.1 N NaOH (positive control)."

sparser
"A HET-CAM based vascularized intestine tumor model as a screening platform for nano-formulated photosensitizers."

sparser
"A HET-CAM test conducted by Mahboobian et al. revealed that the cumulative score for the acyclovir in situ ocular gel formulation was 0.33."

sparser
"Finally, the HET-CAM test proved the non-irritative potential of NE ASE ."

sparser
"Based on the similarities of vasoactive effects observed in HET-CAM test, we suggest that the venom from B. arietans and B. parviocula may share certain venom protein families’ responsible for haemotoxicity, such as metalloproteinases and disintegrins, C-type lectins and snaclecs."

sparser
"HET-CAM is an inexpensive, short-term in vivo method for predicting the ocular irritant effect of chemicals as an alternative to the classic Draize test in rabbits [ xref , xref ]."

sparser
"These results also corroborate previous observations concerning the applicability of HET-CAM as an alternative method to assess irritation to the administration site (Eichenbaum et al., xref )."

sparser
"As observed by others [ xref ], certain metal elements present in an extraction medium might influence their cytotoxicity, but not necessarily represent an irritant material when evaluated in an in vivo HET-CAM setting."

sparser
"In addition, ocular irritation of the sparfloxacin in situ ophthalmic formulation was assessed by the HET-CAM test."

sparser
"Based on interlaboratory validity, which was conducted by Hagino in 1999, the HET-CAM method can provide an alternative method to evaluate potential irritation of chemicals to the eye."

sparser
"The non-irritant character of the two compounds revealed by the HET-CAM assay indicated their biocompatibility with mucosal tissues thus enabling their biomedical use ( xref )."
| PMC

sparser
"The optimized formulation exhibited a higher % of growth inhibition on A-431 cells and demonstrated good anti-angiogenic activity in the HET-CAM test."

sparser
"CHNPs did not exhibit any sign of damage to excised goat cornea, which is confirmed by hydration, histopathology, and HET-CAM test."

sparser
"The in vivo test was assessed considering taking into account that each of the formulation compounds was safe and biocompatible, based on the previously performed in vitro HET-CAM test and analysed by other authors [ xref , xref ]."
| PMC

sparser
"After accelerated physical stability and bovine conrneal permeation studies, NE2 was chosen as optimized formulation forantimicrobial efficacy, and hen's egg test-chorioallantoic membrane (HET-CAM) tests."

sparser
"The safety and non-irritant potential of CNF as a nasal delivery was confirmed in in vitro HET-CAM analysis."

sparser
"HET-CAM test showed no signs of hemorrhage, coagulation, or lysis for the loaded nanoemulgel same as sodium chloride solution (negative control) where its irritation score was zero compared to 9.87 for the positive irritant group (1%w/v sodium lauryl sulfate)."

sparser
"The irritation of polyglyceryl-10 monocaprylate evaluated by HET-CAM at the concentration of 4 wt% was moderate irritant (irritation score = 8.4), which was lower than that of PEG-6 caprylic/capric glycerides (severe irritant, irritation score = 14.1)."

sparser
"The nanosystem presented a zeta potential equal to - 30.1 ± 1.8 mV and was stable for at least 90 days when stored at 6°C. The colloidal aqueous dispersion was non-irritating, according to the in vitro HET-CAM test."

sparser
"An ocular irritation study of innovative besifloxacin in situ gel in comparison with sodium hydroxide (NaOH) and normal saline was performed using the HET-CAM test."

sparser
"To the in vitro safety assessment CAM-TBS, HET-CAM, and red blood cell tests were achieved."

sparser
"Recently, NE loaded P. emarginatus essential oil demonstrated high kinetic stability and was non-irritating, estimated by HET-CAM [ xref ]."

sparser
"Moreover, the safe application of MoNoS gas plasma jet adapters was confirmed by their similar-to-superior safety profiles assessed in the hen's egg chorioallantoic membrane (HET-CAM) coagulation and scar formation irritation assay."

sparser
"In addition, no potential eye irritation was observed in the HET-CAM assay."

sparser
"The product that was found to be non-safe in the product use clinical evaluation was also unsuitable by most of the HET-CAM protocols."

sparser
"The 5-fold increased effect of KT-NP formulation on collagen gene expression compared to KT is also related to the enhanced anti-inflammatory effect in line with the in vivo HET-CAM assay results."

sparser
"In this review, we highlight relevant studies in the field and spotlight the applicability of alternative models, including C. elegans , D. rerio , Drosophila , HET-CAM and Lab-on-a-chip."

sparser
"It was the objective of this proof-of-principle study to evaluate the feasibility of the combination of high-resolution MR imaging and highly sensitive PET imaging in ovo in a HET-CAM xenograft model system for evaluation of target-specific radiotracer accumulation."

sparser
"Hydrogels exhibited properties for topical administration and did not induce irritation effects on the HET-CAM."

sparser
"In ovo studies (HET-CAM) revealed that the formulation can be considered nonirritant. (4) Conclusions: Therefore, NLC systems are a promising approach for the ocular delivery of MGN."

sparser
"The cytotoxicity analysis of EOs and d-limonene was carried out with MRC-5 and HaCaT, and the preliminary safety profile was also evaluated by the HET-CAM assay."

sparser
"The toxicity of the prepared microgels was evaluated by cell line and HET-CAM test, which demonstrated no toxic effect of the prepared microgels."

sparser
"Since no approval for animal testing is required in most countries, a study based on HET-CAM can start much faster than a comparable study in mice."

sparser
"All the products that were classified as non-irritant by any HET-CAM protocols were also safe in the product use clinical study."

sparser
"The Hen's egg test on chorioallantoic membrane (HET-CAM) model provides the generation of vascularized tumor spheroids and therefore, is an ideal test platform which can be used as an intermediate step between in vitro analysis and preclinical evaluation in vivo."

sparser
"HET-CAM is a well-accepted ex vivo conjunctival irritation model that produces responses (of hyperemia, hemorrhage, and clotting/coagulation) to test substances similar to those involving the conjunctiva of the eye. xref shows developmental stages of the CAM at 3- and 10-days and different irritant responses to the moderate irritant propylene glycol (PG), a strong irritant (sodium hydroxide 1 M), and F-P3/CS1. xref shows the cumulative numerical irritation scores of the test substances and controls."

sparser
"The favorable zero-order kinetics that prolonged capsaicin release and the significantly faster transdermal penetration of the NLC attributed to the reduction in skin irritation of the concentrated capsaicin NLCs, as illustrated by the in vitro EpiDerm TM three-dimensional human skin irritation test and hen’s egg test chorioallantoic membrane assay (HET-CAM)."

sparser
"Through the HET-CAM test, the formulations were classified as non-irritating."

sparser
"With the capability of MRI to provide multiple image contrasts even in the small HET-CAM tumors [ xref ], its potential to provide further important physiological and molecular information (e.g., perfusion, diffusion, and tissue relaxation properties) for a more detailed tumor characterization is of major interest for future studies."

sparser
"We assayed the effects of venoms from the most widespread venomous African Bitis arietens and closely related species Bitis parviocula using The Hen’s Egg Test—Chorioallantoic membrane test (HET-CAM) and Chicken embryotoxicity screening test (CHEST), acetylcholinesterase (AChE) analysis, cytotoxicity assay performed on cell lines and protein analysis of selected venoms."

sparser
"The results of the in vivo anti-inflammatory activity tested by the HET-CAM assay showed that the system had good anti-inflammatory potential compared to free dKTP and identical to free dKTP at one-fifth the dose [ xref ]."

sparser
"HET-CAM Test ( Hen’s Egg Test—Chorioallantoic Membrane ) ."

sparser
"In addition, the potential irritant effect of the two cytostatics on the vascular plexus was also examined in ovo, by employing the chick chorioallantoic membrane (HET-CAM) assay."

sparser
"The hydrogels were characterized in terms of swelling, light transmission, mechanical properties, eye compatibility (hen’s egg test on chorioallantoic membrane (HET-CAM) assay), and capability to load and release the antiviral drugs."

sparser
"This proof-of-principle study shows the general feasibility of the HET-CAM xenograft model for target-specific imaging with PET and MRI."

sparser
"Irritations at risk of vascular damage, such as bleeding, lysis, and coagulation, can be evaluated by HET-CAM test, which is optimal for the study of compound accuracy and safety for ocular application. xref , xref "

sparser
"In addition, the potential irritating effect on the vascular plexus were highlighted by applying the in ovo method, chick chorioallantoic membrane (HET-CAM)."

sparser
"HET-CAM results also confirmed its non-irritant potential (zero score)."

sparser
"For eye irritation and toxicity, the hen’s egg test on chorioallantoic membrane (HET-CAM) has been used, which is performed by exposing the CAM of the fertilized chicken egg, as shown in xref ."

sparser
"The present form of the HET-CAM test can be proposed as a pre-screen method of eye irritation tests, therefore the number of test animals can be reduced."

sparser
"It did not show any toxicity to the tested tissue, confirmed by corneal hydration (77.3%), cornea histopathology (no internal changes), and a HET-CAM test (zero score)."

sparser
"The toxicity of the compounds was investigated by means of HET-CAM assay ( xref ), which is a reliable Ex vivo test used to mimic in vivo inflammatory reactions and recognized as a predictive model for the irritant effect on conjunctival tissues [ xref ]."
| PMC

sparser
"In vitro SUN release profiles were obtained for the optimal formulations, along with ex vivo corneal permeability/retention studies, and ocular tolerance assays, namely: the bovine corneal opacity and permeability (BCOP) assay, the HET-CAM test (hen's egg test - chorioallantoic membrane), and hemolytic potential (HP) assay."

sparser
"Good ocular tolerance of the formulation was observed both in an HET-CAM test, where no sign of vascular response was observed, and in a Draize test, performed on New Zealand white rabbits where no sign of inflammation or excessive lacrimation was observed [ xref , xref ]."

sparser
"For this purpose, we applied our recently established HET-CAM [ xref , xref ]."

sparser
"The results obtained from the HET-CAM investigation didn't show any sign of vessel injury on the CAM surface for prepared formulation."

sparser
"Hen's egg test on the chorioallantoic membrane (HET-CAM) HET-CAM assay has been accepted as an alternative model for in vivo tests because of the high sensitivity of the chorioallantoic membrane to show damages in the presence of irritant compounds."

sparser
"HET-CAM was carried out to test ocular irritation and revealed no signs of membrane discoloration or hemorrhage."

sparser
"In addition to the in vitro skin irritation test, the in ovo HET-CAM method was employed."

sparser
"Moreover, BSA-NPs were nonirritant as shown by HET-CAM test."

sparser
"A Novel Phytotherapy Application: Preparation, Characterization, Antioxidant Activities and Determination of Anti-inflammatory Effects by in vivo HET-CAM Assay of Chitosan-based DDSs Containing Endemic Helichrysum pamphylicum P.H. Davis & Kupicha Methanolic Extract."

sparser
"The results of the HET-CAM test provide a detailed insight into previously undescribed microscopic changes of CAM vessels induced by Bitis parviocula venom ( xref , xref and xref )."

sparser
"In this regard, the authors performed in vitro (characterization of the hydrogels, drug release and cell toxicity) and ex vivo (transcorneal permeation and Hen´s Egg Chorioallantoic membrane (HET-CAM)) assays."

sparser
"Irritation tests of the chili extract-loaded lipid-based nanoparticle and the gel formulation containing the chili extract-loaded lipid nanocarrier were performed using the hen’s egg test chorioallantoic membrane (HET-CAM) assay and the in vitro skin irritation EpiDerm TM test model, respectively."

sparser
"Formulations were characterized with respect to dillapiole content, droplet size, polydispersity index, zeta potential, morphology, rheological behavior, bioadhesion, skin permeation profile, and in vitro irritancy (HET-CAM)."

sparser
"Therefore, the HET-CAM test was well validated in the present study."

sparser
"Among them, cell and tissue culture models, the hen’s egg test on the chorioallantoic membrane (HET-CAM) as well as the Caenorhabditis elegans ( C. elegans ) model have gained considerable attention as alternative screening platforms to evaluate the toxicity of bioactive compounds in various context (Haselgrubler et al. xref ; Hunt xref ; Piersma xref ; Scheel et al. xref )."

sparser
"MZBSoG2 was found to be a non-irritant because it achieved a score of zero (standard score) in the HET-CAM test."

sparser
"HET-CAM tests did not suggest any potential for ocular irritation of both uncoated and coated samples."
| PMC

sparser
"The techniques used to examine the safety profile of those polymers include the HET-CAM test, gamma scintigraphy study, handheld infra-red (IR) assay, histopathology study, and in vivo eye irritation assay."

sparser
"Chorioallantoic membrane (HET-CAM) tests were carried out to predict potential ocular irritation."
| PMC

sparser
"HET-CAM of Bolalipid Hydrogels."

sparser
"By now, several alternative parameters like lysis, hyperemia and vasoconstriction have been evaluated using adapted HET-CAM protocols."

sparser
"In vitro ocular tolerance was studied by HET-CAM test."
| PMC

sparser
"Thus, this study proposed to apply two strategies for the prediction of the eye irritation potential of different concentrations of surfactants and silicones, the first one involving evaluation Hen's Egg Test - Chorioallantoic Membrane (HET-CAM), and the other one using Bovine Corneal Opacity and Permeability (BCOP) followed by histopathological."

sparser
"Olopatadine-loaded CL successfully passed the HET-CAM eye irritation test and showed good mast cell compatibility."
| PMC

sparser
"The battery of selected methods comprised regulatory accepted EpiDerm™ skin model (OECD TG 439); EpiOcular™ corneal model (OECD TG 492) and scientifically valid test method HET-CAM (DB-ALM Protocol No."

sparser
"Multi-Modal PET and MR Imaging in the Hen's Egg Test-Chorioallantoic Membrane (HET-CAM) Model for Initial"

sparser
"The results of the reconstructed human epidermis model, human corneal epithelium model and consumer research showed that no irritation was found in any test products; however, HET-CAM tests showed positive results."

sparser
"The irritant effects can be observed in the CAM of fertile eggs following the exposure to a substance in the HET-CAM test which is analogous to the Draize rabbit-eye test [ xref ]."

sparser
"The purpose of this study was to compare the different protocols of the HET-CAM, the French and German protocols, by evaluating the eye irritation potential of surfactants."

sparser
"The existence of the HET-CAM test can be an initial test for eye irritation tests so that the use of animals in toxicological tests can be reduced [ xref ]."

sparser
"Mucosa compatibility study of hydrogels was performed using HET-CAM test [ xref ], which replaced the Draize rabbit eye test [ xref ]."

sparser
"All hydrogels showed physical properties adequate for CLs and successfully passed the hen’s egg-chorioallantoic membrane (HET-CAM) test."
| PMC

sparser
"NTX-loaded hydrogels were highly cytocompatible with human mesenchymal stem cells (hMSC) and passed the HET-CAM test."

sparser
"Ocular irritation studies were carried out using the HET-CAM method in order to ascertain the ophthalmic tolerability of the prepared formulations."

sparser
"The designed mini-implant alloy, exposed to the perfused chorioallantoic membrane, showed no sign of irritation and no alteration of vascular parameters in the HET-CAM assay ( xref )."

sparser
"HET-CAM Model ensured the non-irritant nature of CS-5-FU-NLCs."

sparser
"Hen’s egg-chorioallantoic membrane (HET-CAM) test was applied showing that the positive controls (NaOH 1 M) resulted in severe hemorrhage, which increased over five minutes grading this solution as a severe irritant."

sparser
"Besides, the extract was safe since it induced no irritation in the HET-CAM test."

sparser
"Vasoactive effects were visualized using the Hen's Egg Test - Chorioallantoic membrane (HET-CAM) test."

sparser
"A Hen’s Egg Test-Chorioallantoic membrane (HET-CAM) test revealed that the gel was slightly irritative [ xref ]."

sparser
"HET-CAM assessment is a well-established in-vitro parameter for determination of ocular tolerability of the test sample, xref and scores are depicted in xref ."

sparser
"In addition, potential adverse effects of the hydrogels were determined using the &quot;Hen's egg test on chorioallantoic membrane&quot; (HET-CAM) test and a mouse model."

sparser
"The hen’s egg chorioallantoic membrane test (HET-CAM) showed that blood vessels were neither expanded nor shrunk after treatments with myricetin, blank-LNP, and M-LNP (Fig.  xref c,d)."

sparser
"All hydrogels showed physical properties adequate for CLs and successfully passed the hen's egg-chorioallantoic membrane (HET-CAM) test."

sparser
"HET-CAM is a widely used test to determine the irritancy potential of the developed formulations or test substances."

sparser
"Achyrocline satureioides (Lam.) DC (Asteraceae) Extract-Loaded Nanoemulsions as a Promising Topical Wound Healing Delivery System: In Vitro Assessments in Human Keratinocytes (HaCaT) and HET-CAM Irritant Potential."

sparser
"HET-CAM Irritation Study."

sparser
"The result of in vitro toxicity test by the HET-CAM test method is appropriate with the other in vitro toxicity test results."

sparser
"Herein, we studied ex vivo permeation, penetration, anti-fungal activity, and Hen's Egg Test-Chorioallantoic Membrane (HET-CAM) toxicity tests in order to assess ocular targeting of PSC micelles, which were optimized in our previous study."

sparser
"The results correlate the composition and topography of the implant surface with biological experimental evaluations related to directly affected cells (gingival fibroblasts) and toxicological results on blood vessels (hen's egg test-chorioallantoic membrane (HET-CAM) assay)."

sparser
"Irritation of PGCE-1 was evaluated by HET-CAM."

sparser
"In Germany and France, HET-CAM has been officially accepted as a valid in vitro assay, at least for predicting several irritating substances [ xref ]."

sparser
"As far as we know, these two volatile oils ( H. rhamoides and C. citratus ) have not been tested for the irritating effect by employing the HET-CAM method."
| PMC

sparser
"The effect of the hydrogels on the metabolic activity, proliferation and apoptosis of fibroblast (MRC-5, BJ, L929), retinal pigment epithelial (ARPE-19, hiPSC-derived RPE) and photoreceptor cells (661W) was studied as well as their mucosal tolerance via a HET-CAM assay."

sparser
"Evaluation of CNF Irritancy Potential on HET-CAM."

sparser
"The microemulsion was investigated for the irritation properties in comparison with nonencapsulated OEO using HET-CAM (hen egg test-chorioallantoic membrane) assay."

sparser
"NC-FA and NC-B were able to interact with mucin, which is an indicative of mucoadhesive properties and the association of FA with nanocapsules showed decreased irritation by HET-CAM method."

sparser
"HET-CAM Test."

sparser
"Liposomes elaborated with phosphatidylcholine (LP PC) or phosphatidylcholine and spermine (LP PC: SPM) were stable under storage at 6 ºC for 30 days, showed mucoadhesive characteristics, and were non-irritant, according to HET-CAM tests."

sparser
"An in vivo hen's egg-chorioallantoic membrane (HET-CAM) angiogenic study was carried out for 21 compounds 6a , b , d , f , h , i , k-o , t , and 6x to check their mortality and toxicity."

sparser
"The non-irritant behavior of the Soluplus and Pluronic F68 micelle dispersions is in good agreement with previous reports on HET-CAM assay results recorded for 23% w / v Soluplus dispersions [ xref ] and in situ gel formulations prepared with 23% w / v Pluronic F127 and 15% w / v Pluronic F68 [ xref ]."

sparser
"At this concentration, CW was safe for topical applications since it induced no irritation signs on the CAM in HET-CAM test."

sparser
"The biocompatibility of irrigant solutions, including 8.25% sodium hypochlorite (NaOCl), was analyzed in samples (n = 25) of chorioallantoic membranes (CAM) from embryos of the Gallus gallus, using the hen's egg test on chorioallantoic membrane (HET-CAM)."

sparser
"We also reported for the first time the in vitro toxicity test using the Hen’s Egg Test Chorioallantoic Membrane (HET-CAM) test method."