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USP11 binds NFKBIA. 13 / 14
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reach
"The association between USP11 and IkappaBalpha were also confirmed by co-immunoprecipitation of endogenous IkappaBalpha and USP11 in HeLa cells (XREF_FIG)."
19874889
sparser
"Together, these results demonstrate that USP11 is specifically and constitutively associated with IκBα in the cells and both USP11 N-terminal regulatory domain and C-terminal USP domain are required for its association with IκBα."
19874889
sparser
"In conclusion, our data provide evidence of the physical and functional interaction between IκBα and USP11."
19874889
reach
"These results suggest that binding of USP11 with IkappaBalpha is required for USP11 mediated IkappaBalpha deubiquitination."
19874889
reach
"To determine whether the binding of USP11 with IkappaBalpha is required for its deubiquitinase activity toward IkappaBalpha, we co-transfected Flag-IkappaBalpha with Myc-USP11 full-length, Myc-USP11-NT or Myc-USP-CT into HEK 293T cells."
19874889
sparser
"In addition, we found that only USP domain failed to mediate the association of USP11 with IκBα and deubiquitination of IκBα."
19874889
sparser
"To rule out the possibility that association of USP11 with IκBα is through other proteins, we co-incubated recombinant His-USP11 with either recombinant GST-Control or GST-IκBα in vitro and found that GST-IκBα but not GST-Control pulled down His-USP11 ( xref )."
19874889
reach
"In conclusion, our data provide evidence of the physical and functional interaction between IkappaBalpha and USP11."
19874889
sparser
"To determine whether the binding of USP11 with IκBα is required for its deubiquitinase activity toward IκBα, we co-transfected Flag-IκBα with Myc-USP11 full-length, Myc-USP11-NT or Myc-USP-CT into HEK 293T cells."
19874889
sparser
"These results suggest that binding of USP11 with IκBα is required for USP11-mediated IκBα deubiquitination."
19874889
sparser
"However, in our co-transfection assay, we found that USP11 but not USP15 is associated with IκBα."
19874889
sparser
"We present evidence that USP11 is constitutively associated with IκBα and attenuates IκBα degradation to negatively regulate TNFα-induced NF-κB activation."
19874889
sparser
"USP11 is associated with IκBα."
19874889