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CAV3 activates KCNMA1. 22 / 22
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"As a result, Cav3 activation of KCa1.1 channels extends the voltage range for KCa1.1 activation beyond that provided by HVA calcium channels."
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"XREF_BIBR and the current study support experimental results in predicting that multiple Cav3 channels are necessary to cause significant KCa1.1 activation."
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"XREF_BIBR We also hypothesized that multiple Cav3.2 channels may cooperate to activate KCa1.1 channels."
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"Yet unlike HVA channels, the Cav3 mediated activation of KCa1.1 channels was sensitive to intracellular EGTA in tsA-201 cells and some MVN cells."
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"To gauge the physiological effects of Cav3 activation of KCa1.1 channels we recorded the membrane potential and spike response of MVN cells under current clamp conditions."
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"The widespread expression of these channel subtypes suggest that a Cav3 mediated KCa1.1 regulation may be relevant to the control of membrane excitability in a wide range of cell types."
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"To gauge the physiological effects of Cav3 activation of KCa1.1 channels we recorded the membrane potential and spike response of MVN cells under current clamp conditions."
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"This was further supported by a Cav3 dependent activation of KCa1.1 current in the form of a low voltage activated and fast inactivating KCa1.1 current."
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"Cav3 mediated KCa1.1 Activation in situ."
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"But Cav3 mediated activation of KCa1.1 is less reliable in being blocked by as little as 5mM internal EGTA in both tsA-201 and MVN cells."
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"Hence, the concerted action of multiple Cav3 channels may be needed to activate KCa1.1."
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"Expression of the channel α-subunits alone in tsA-201 cells was sufficient to enable Cav3 activation of KCa1.1 current."
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"XREF_BIBR Paradoxically, the Cav3 activation of KCa1.1 current could be blocked with as little as 5 mM EGTA, a result usually interpreted as reflecting a microdomain interaction between subunits."
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"Cav3 Channel Activation of KCa1.1 Current."
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"As a result, Cav3 activation of KCa1.1 channels extends the voltage range for KCa1.1 activation beyond that provided by HVA calcium channels."
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"Expression of the channel alpha-subunits alone in tsA-201 cells was sufficient to enable Cav3 activation of KCa1.1 current."
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"Cav3.2 Activation of KCa1.1 Current in the tsA-201 Expression System."
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"Using a ramp command over a -100 to -30 mV range with paxilline or mibefradil treatment further revealed LVA outward current that activated just subsequent to LVA inward calcium current, indicating that Cav3 calcium influx can activate KCa1.1 above ~-70mV."
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"Moreover, the Cav3 mediated activation of KCa1.1 confers the unique voltage profile of Cav3 calcium channels upon KCa1.1 current, extending the potential voltage range for KCa1.1 activation to both the low and high voltage realms of membrane potential deflections."
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"A computational model of the KCa1.1 and Cav3 complex suggested that multiple Cav3 channels were necessary to activate KCa1.1 channels, potentially causing the KCa1.1 and Cav3 complex to be more susceptible to calcium chelators."
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"In tsA-201 cells a single pulse from -100 mV to +40 mV recruited very little KCa1.1 current while in MVN cells a step of this size consistently recruited sufficient Cav3 calcium current to activate KCa1.1 channels."
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"We then used patch clamp recordings in in vitro tissue slices from P12-P16 rats to examine if Cav3 calcium influx is sufficient to activate KCa1.1 in MVN cells."
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