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"Healthy mandarin fish (weight ∼250 g) were obtained from a fish farm in Nan-Hai (Guangdong Province, China) and maintained for at least 2 weeks in aquaria at 28 °C. Freshly dissected tissues, including brain, fat tissue, heart, hemocytes, gills, intestines, liver, muscle, kidney, and spleen, were washed thoroughly with phosphate buffered saline (PBS) and immediately homogenized with thiourea lysis buffer (7 M urea, 2 M thiourea, 4% CHAPS) containing protease inhibitor cocktail set III (Calbiochem, USA)."