IndraLab

Statements

KCNMA1 binds SPN. 14 / 14
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sparser
"Therefore, there must be a strict temporal or localization requirement for how SPN and SLO interact with the membrane that dictates which mode of membrane binding SLO will adopt, as illustrated in the proposed model ( xref )."
26059530
sparser
"It is possible that the interaction of SPN and SLO at the membrane contributes to perturbations leading to inhibition of pore healing that may occur in the absence of SPN."
26838722
sparser
"SPN-dependent binding of SLO is sufficient for pore formation during infection."
26059530
sparser
"Infections were also performed on Lec1 cells to further characterize the putative carbohydrate receptor for SLO, and to assess whether insensitivity to SLO-mediated pore formation in the absence of SPN is a general phenotype of Lec derivative cell lines."
26059530
sparser
"We previously showed that polymorphisms in the upstream region result in enhanced production of SPN and SLO, which is strongly associated with increased transmission and virulence of epidemic M1 and [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
28034602
sparser
"The interaction of Spn with SLO can modify SLO membrane binding properties [ xref ]."
30577571
sparser
"However, it has been established that SPN and SLO are functionally associated."
28034602
sparser
"These findings demonstrate that while a glycan receptor likely mediates SLO binding in the absence of SPN, it is not required for SLO binding during wild-type infection, nor does it contribute to SPN translocation."
26059530
sparser
"Additionally, while data suggest that SPN and SLO interact at the host cell membrane ( xref ), complex formation has not been detected in solution."
26059530
sparser
"Surprisingly, the lytic activity of the SLO variants requires the co-expression of SPN, indicating that in the absence of glycan binding by SLO, the SPN-dependent interaction of SLO at the host cell membrane is sufficient for pore formation during infection."
26059530
sparser
"The variant 3 promoter region is associated with elevated production of SPN ( S. pyogenes NADase) and SLO (streptolysin O), two potent secreted cytolytic toxins that contribute to virulence ( xref )."
26443457
sparser
"SPN may exhibit higher avidity binding in vivo if SLO and SPN associate at the membrane as a complex that allows for oligomerization of SLO, which may cluster SPN to strengthen a putative SPN-carbohydrate interaction."
26059530
sparser
"In this study, we have expanded our understanding of how SPN and SLO interact to produce cellular responses that neither produces in isolation and how their synergy is influenced by SPN diversity."
26838722
sparser
"The data presented in this study suggest that when SLO is unable to associate with SPN, glycan recognition by SLO serves a similar function to ILY-CD59 binding and is required for SLO to become properly oriented at the membrane for pore formation."
26059530