IndraLab
Statements
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"Consistent with the observation that enzymatic inhibition of USP7 resulted in ECT2 destabilization , treatment of HeLa cells with USP7 inhibitor GNE-6640 for 4 h resulted in a marked increase in the level of ubiquitinated ECT2 species and a decrease of total ECT2 ( Figure 5J and S4C ) ."
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"These results indicated that USP7 targets ECT2 for deubiquitination, indicating that ECT2 is a bona fide substrate of USP7."
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"To further support this deduction, the potential of USP7 to modulate the steady-state level of ECT2 protein was assessed by cycloheximide (CHX) chase assays."
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"To gain molecular insights into the functional connection between USP7 and ECT2, we examined whether USP7 promoted ECT2 stabilization is dependent on its ubiquitin specific protease activity."
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"Given that ECT2 is an ubiquitinated protein XREF_BIBR, XREF_BIBR and USP7 promotes the stabilization of ECT2, we next asked the question whether USP7 functions to deubiquitinate ECT2."