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Arachidonic acid activates KCNMA1. 31 / 31
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"We observed that there is a discrepancy in this experimental model between the effects of miconazole and SKF525A, another frequently used CYP inhibitor, on AA-mediated BK channel activation, carbacycl[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"5-Lipoxygenase metabolites of arachidonic acid stimulate BK channel activity in GH 3 cells through a calcium-independent mechanism involving a hormonally activated protein phosphatase that causes deph[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Arachidonic acid (AA) dissolved in DMSO activated the BK channel in a dose dependent manner (from 0.5 to 10 microM), and DMSO (0.1%) alone had no effect on the activity of the BK channel."
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"Arachidonic acid (AA) dissolved in DMSO activated the BK channel in a dose dependent manner (from 0.5 to 10 microM), and DMSO (0.1%) alone had no effect on the activity of the BK channel."
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"Although higher concentrations of ETI had no effect on BK channel gating in cell-free patches in the absence of exogenous ATP to support protein phosphorylation (not shown), BK channel stimulation by [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"The concentration of Ca2+ and Mg2+ in the bathing solution had no appreciable effects on the AA induced increase of BK channel activity."
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"BK channel stimulation by arachidonic acid under these conditions did not result from an increase in intracellular calcium nor from stimulation of protein kinase C (Keyser and Alger, 1990)."
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"BK channel stimulation by arachidonic acid was confirmed in recordings from cell attached patches, in which the BK channels were identified by their characteristic large-conductance (120 pS) and volta[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Thus, lipoxygenase metabolites of arachidonic acid stimulate BK channel activity in GH cells through a calcium independent mechanism involving a protein phosphatase."
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"In conclusion, these results strongly suggest that BK channel activation by AA requires the expression of β1, β2, or β3 subunits."
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"We present evidence that AA activates BK channel in a direct way, inducing VSD stabilization on its active configuration observed as a significant left shift in the Q-V curve obtained from gating currents recordings."
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"AA enhances KCa1.1 currents only when the channel contains beta2 or beta3 subunits [XREF_BIBR, XREF_BIBR]."
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"Also, AA and LOM potentiation of BK channel activity in outside-out patches exposed to strong Ca2+ buffering ruled out cytoplasmic messengers or changes in intracellular Ca2+ levels as causative factors."
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"Because arachidonic acid does not relieve BK channel block by TEA (Figs. 2 and 3 and 10), these results indicate that TBA is not directly inhibiting BK channel activity, but is acting upstream of arac[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Finally, BK channel activation by arachidonic acid and analogues has recently been linked to the ability of these FAs to remove beta 2 - or beta 3 -mediated BK inactivation."
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"Nevertheless, BK channel stimulation by arachidonic acid was only partially reduced from a 442% increase on control cells down to 344% by acutely blocking calcium entry through voltage dependent chann[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"In contrast, treating the RA-FLS with 30muM AA, known to enhance KCa1.1 currents only in the presence of beta2 or beta3 subunits [XREF_BIBR], induced an increase in current amplitude in the majority of the cells."
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"In conclusion, these results strongly suggest that BK channel activation by AA requires the expression of beta1, beta2, or beta3 subunits."
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"Similarly, arachidonic acid (AA) increases BK channel currents in the presence of β2 subunits [223] and AA metabolites produced by a COX-mediated pathway are responsible for BK channel activation in microglia [8]."
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"Similarly, arachidonic acid (AA) increases BK channel currents in the presence of β2 subunits [223] and AA metabolites produced by a COX-mediated pathway are responsible for BK channel activation in microglia [8]."
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"The cis FFA, palmitoleic, oleic, linoleic, linolenic, eicosapentaenoic, and the triple bond analog of AA, eicosatetraynoic acid, all increased BK channel activity, whereas stearic (saturated) or the trans isomers elaidic, linolelaidic, and linolenelaidic had no effect."
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"AA also activates potassium current trough KCa1.1, however, there was no potassium ion neither in the intracellular or the extracellular solution thereby excluding the contamination of the whole-cell current by KCa1.1."
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"Application of arachidonic acid increased BK channel activity, and this occurred to a greater extent in rats on a high-K diet compared with a normal-K diet."
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"AA also activates potassium current trough KCa1.1, however, there was no potassium ion neither in the intracellular or the extracellular solution thereby excluding the contamination of the whole-cell current by KCa1.1."
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"KCa1.1 was first activated by 30microM AA then inhibited fully by paxillin (the subunit composition independent inhibitor of the KCa1.1 channels)."
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"We present evidence that AA activates BK channel in a direct way, inducing VSD stabilization on its active configuration observed as a significant left shift in the Q-V curve obtained from gating currents recordings."
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"For instance, DHA, or AA augmentation of the Slo1 channel is potentiated or enabled, respectively, when the channel is co-expressed with specific β subunits (Sun et al., 2007; Hoshi et al., 2013a)."
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"The co-existence of AA-induced activation of both KCa1.1 and the hHv1 current was also tested using K + -containing solutions (Supplementary Fig.  xref )."
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"KCa1.1 was first activated by 30 µM AA then inhibited fully by paxillin (the subunit composition-independent inhibitor of the KCa1.1 channels)."
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"Discussion The present study provided direct evidence that four regioisomeric EETs, metabolites of arachidonic acid via the cytochrome P450 pathway, potentiated the BK channel activity with a concomit[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Both arachidonic acid and nitric oxide potentiate BK channel activity by slowing inactivation kinetics and enhancing channel opening, respectively [XREF_BIBR, XREF_BIBR]."
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