IndraLab
Statements
sparser
"Consistent with the evaluation of CLR-ethanol interaction on myogenic tone and the key role of BK channels in mediating ethanol-induced cerebral artery constriction ( xref ), electrophysiology on membrane patches excised from rat and mouse cerebral artery myocytes reveals that ethanol-induced BK channel inhibition is lost after CLR-depleting treatment."
sparser
"In the case of β1 subunit-containing BK channels, the similar ability of cholesterol and ent -cholesterol to enhance BK channel function may hold the key to unraveling the precise mechanism by which both steroids blunt alcohol-induced BK channel inhibition and the resulting cerebral artery constriction ( xref – xref )."
reach
"However, the effect of cholesterol on ethanol induced vasoconstriction is not monotonic : we have previously reported that cholesterol depletion from rat and mouse cerebral arteries blunts ethanol induced BK channel inhibition and the resulting cerebral artery constriction [XREF_BIBR]."
reach
"In the case of beta1 subunit containing BK channels, the similar ability of cholesterol and ent-cholesterol to enhance BK channel function may hold the key to unraveling the precise mechanism by which both steroids blunt alcohol induced BK channel inhibition and the resulting cerebral artery constriction (XREF_FIG - XREF_FIG)."
reach
"To ensure that cholesterol blunting of ethanol induced BK channel inhibition is present when ethanol induced inhibition is comparable with inhibition observed in myocytes from wt mice, we performed experiments at higher levels of calcium that would enable ethanol induced BK channel inhibition in the absence of BK beta1 subunits [XREF_BIBR]."
sparser
"Although age-dependent increase in BK β 1 subunit protein level and function paralleled the increase in ethanol-induced constriction and ethanol-induced BK channel inhibition, additional molecular players that could contribute to age-dependent susceptibility of cerebral arteries to ethanol cannot be ruled out."
reach
"If a cholesterol driven decrease in ethanol partition coefficient into the membrane underlies cholesterol antagonism of ethanol 's effect on BK channel function and cerebral artery diameter, then the described ethanol sensing site seems unlikely to play a major role in the ethanol induced BK channel inhibition that was observed in our experiments and that is routinely reported in the presence of beta1 subunits and high levels of Ca 2+ i [XREF_BIBR, XREF_BIBR]."
reach
"Although age dependent increase in BK beta1 subunit protein level and function paralleled the increase in ethanol induced constriction and ethanol induced BK channel inhibition, additional molecular players that could contribute to age dependent susceptibility of cerebral arteries to ethanol can not be ruled out."
reach
"Consistent with the evaluation of CLR-ethanol interaction on myogenic tone and the key role of BK channels in mediating ethanol induced cerebral artery constriction, electrophysiology on membrane patches excised from rat and mouse cerebral artery myocytes reveals that ethanol induced BK channel inhibition is lost after CLR depleting treatment."
sparser
"The similar ability of cholesterol and ent -cholesterol to antagonize ethanol-induced BK channel inhibition and resulting cerebral artery constriction argues against the idea that cholesterol control over an alcohol effect requires cholesterol recognition by a protein site that specifically recognizes natural cholesterol."
sparser
"We used inside-out patches with Ca 2+ free in the bath solution set at 30 μ M. This concentration of Ca 2+ is reached in the vicinity of cerebral artery myocyte BK channel during myocyte constriction and is known to favor ethanol-induced inhibition of the cerebral artery BK channel [ xref , xref ]."
reach
"Considering that cholesterol antagonism of ethanol 's effect was found in cell-free myocyte membranes, cholesterol protection against ethanol induced BK channel inhibition and resulting vasoconstriction are independent of freely diffusible cytosolic signals and cell metabolism of cholesterol or ethanol."
sparser
"However, the fact that increase in CLR level by 10–15% drove notable pharmacological consequences, argues in favor of a mechanism that is highly sensitive to CLR levels and/or being able to amplify CLR effect on alcohol-induced BK channel inhibition and resulting vasoconstriction."
sparser
"Considering that cholesterol antagonism of ethanol’s effect was found in cell-free myocyte membranes, cholesterol protection against ethanol-induced BK channel inhibition and resulting vasoconstriction are independent of freely diffusible cytosolic signals and cell metabolism of cholesterol or ethanol."
sparser
"However, the effect of cholesterol on ethanol-induced vasoconstriction is not monotonic: we have previously reported that cholesterol depletion from rat and mouse cerebral arteries blunts ethanol-induced BK channel inhibition and the resulting cerebral artery constriction [ xref ]."
reach
"The similar ability of cholesterol and ent-cholesterol to antagonize ethanol induced BK channel inhibition and resulting cerebral artery constriction argues against the idea that cholesterol control over an alcohol effect requires cholesterol recognition by a protein site that specifically recognizes natural cholesterol."
sparser
"To ensure that cholesterol blunting of ethanol-induced BK channel inhibition is present when ethanol-induced inhibition is comparable with inhibition observed in myocytes from wt mice, we performed experiments at higher levels of calcium that would enable ethanol-induced BK channel inhibition in the absence of BK β1 subunits [ xref ]."