IndraLab

Statements


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sparser
"The overall architecture of TSC1TSC2 and TSC1TBC1D7 interactions together allows one TBC1D7 to be assembled into TSC complex and this subunit stoichiometry is consistent with previous structural and biochemical analyses xref , xref ."

reach
"Our work gives more detailed insight into the structural and functional relationships between TSC1 and TBC1D7, and provides useful information for further studies aimed at unravelling the molecular structure of the TSC1, TSC2, and TBC1D7 complex."

reach
"The TSC1, TSC2, and TBC1D7 complex is an important negative regulator of the mechanistic target of rapamycin complex 1 that controls cell growth in response to environmental cues."

sparser
"When the TSC1-TSC2-TBC1D7 (TSC-TBC) complex is formed, it inhibits mTORC1 activity by means of its GTPase-activating protein (GAP) domain [ xref , xref ]."

sparser
"Active AKT decreases interaction between PRAS40 and mTORC1 and deactivates the tuberous sclerosis complex (TSC) formed by TSC1, TSC2 and TBC1D7 [ xref ]."

sparser
"This suggests that the TSC1-TSC2-TBC1D7 interaction may also be conserved."

reach
"TSC1 is an essential component of the TSC1, TSC2, and TBC1D7 complex."

sparser
"The TSC complex, formed by the subunits TSC1, TSC2, and TBC1D7, is responsible for relaying most growth factor signaling pathways to mTORC1 (Ben‐Sahra & Manning, xref )."

sparser
"Additional immunoprecipitations with antibodies that only recognize TSC2 phosphorylated by Akt on either S939 or T1462, confirmed that TSC1 and TBC1D7 readily associate with phosphorylated TSC2 and remain stably bound even after an hour of insulin stimulation ( xref )."

sparser
"TSC2 forms a protein complex with TSC1 and the recently discovered protein TBC1D7 [ xref ]."

reach
"One of the interacting proteins named TBC1D7 and TSC1/2 heterodimer bind together to form the TSC1, TSC2, and TBC1D7 complex (TSC-TBC) [XREF_BIBR], which regulates the mammalian target of the rapamycin (mTOR) signaling pathway in response to upstream signals such as growth factor stimulation and hypoxia."

reach
"The TSC1, TSC2, and TBC1D7 complex negatively regulates this axis by inhibiting the activation of mTORC1 through a small GTPase protein RHEB XREF_BIBR, XREF_BIBR."

sparser
"Along with TBC1D7, TSC1 and TSC2 form a physical complex that supports the GTPase-activating protein (GAP) activity of TSC2 towards the small GTPase, RHEB, a direct and positive regulator of MTOR (specifically, MTOR complex I or MTORC1) xref ."

No evidence text available

sparser
"AMPK inactivates mTORC1 by at least two mechanisms, i.e. inhibitory phosphorylation of the Raptor subunit that targets the complex to downstream targets and to the lysosome where it is activated, and activatory phosphorylation of TSC2, which forms a key part of the TSC1:TSC2:TBC1D7 complex."

reach
"The TSC1, TSC2, and TBC1D7 complex is the critical negative regulator of mTORC1 signalling with respect to most of its upstream regulators, such as growth factors, insulin and energy levels [XREF_BIBR]."

reach
"Complete loss of either TSC1 or TSC2 inactivates the TSC1, TSC2, and TBC1D7 complex leading to constitutive high-level activation of mTORC1 in TSC tumors."

sparser
"These two proteins function together with TBC1D7 to form a tuberinhamartinTBC1D7 complex by coiled‐coil to restrain mammalian target of rapamycin (mTOR)‐mediated signaling pathway and insulin signaling pathway, thus, playing a role in the regulation of both cell growth, proliferation and differentiation (Dibble et al., xref ; Rosner, Hofer, Kubista, & Hengstschläger, xref ; Tee et al., xref ; Van Slegtenhorst et al., xref )."

sparser
"Tumor suppressors hamartin ( TSC1 ) and tuberin ( TSC2 ) can form an intracellular complex with TBC1 domain family member 7 (TBC1D7) that exerts GTPase-activating protein (GAP) activity towards the small GTPase Ras homolog enriched in brain 1 (RHEB1) (Dibble et al. xref ; Inoki et al. xref )."

sparser
"The interaction of TBC7 with the TSC1TSC2 complex was, however, not altered by the growth factor stimuli such as insulin, epidermal growth factor, and serum (data not shown)."

sparser
"This parallel dimerization of TSC1 leads to an asymmetric formation of TSC1TSC2 tetramer and recruitment of a single TBC1D7 molecule, generating a unique and characteristic modular organization (Fig.  xref and Supplementary Fig.  xref )."

sparser
"Tre2-Bub2-Cdc16 (TBC) 1 domain family, member 7 (TBC1D7) is one of the interacting proteins, which stably binds to the TSC1/2 heterodimer to form the TSC1-TSC2-TBC1D7 (TSC–TBC) complex ( xref )."

sparser
"TSC2 forms a complex with TSC1 and TBC1 domain family member 7 (TSC complex) and acts as a negative regulator of mTORC1 signaling xref , xref , xref ."

sparser
"Signals including growth factors, cellular stresses and energy levels act on the disruption the formation of tuberous sclerosis complex (TSC) complex, comprised of TSC1, TSC2 and TBC1D7, which leads to the translocation and activation of Rheb on the lysosome membrane (Dibble et al., xref )."

sparser
"One of the interacting proteins named TBC1D7 and TSC1/2 heterodimer bind together to form the TSC1-TSC2-TBC1D7 complex (TSC-TBC) [ xref ], which regulates the mammalian target of the rapamycin (mTOR) signaling pathway in response to upstream signals such as growth factor stimulation and hypoxia."

sparser
"The in vivo and in vitro association of TBC7 and the TSC1-TSC2 complex was confirmed by the co-immunoprecipitation and pull-down analysis, respectively, and TBC7 was revealed to bind to the C-terminal half region of TSC1, which is distinct from the binding site with TSC2."

sparser
"Active PKB/Akt phosphorylates TSC2 ( T uberosis S clerosis C omplex 2), and thereby destabilizes the complex formed by TSC1, TSC2 and TBC1D7 ( TBC1 D omain family member 7) ( xref ; xref ; xref )."

reach
"Identification of regions critical for the integrity of the TSC1, TSC2, and TBC1D7 complex."

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"Hence, exogenous expression of TSC1, as effected here by infection with a gene therapy vector can serve to rescue normal levels of the TSC1, TSC2, and TBC1D7 complex."

sparser
"TSC1, TSC2, and TBC1D7 form a heteromeric complex that can bind to and stimulate the GTPase Ras homolog enriched in brain Rheb ( xref ; xref ; xref ; xref ) (for reviews see ( xref ; xref ) ( xref )."

sparser
"The proteins encoded by the TSC1 and TSC2 genes, hamartin and tuberin, respectively, interact with TBC1 domain family member 7 (TBC1D7) to form an active complex that regulates the mTORC1 activation state [ xref – xref ]."

reach
"Insulin activates mTORC1 through the PI3K-Akt pathway, which inhibits the TSC1, TSC2, and TBC1D7 complex (the TSC complex) to turn on Rheb, an essential activator of mTORC1."