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EGF activates ERK. 1000 / 2279
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"In addition, PDGF-BB, EGF and bFGF were recently reported to activate ERK1/2 in different types of hMSCs (Tamama et al., 2006; Zaragosi et al., 2006)."
18583177
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"To explore the timescale at which adaptation occurs, we retriggered ERK activity by a delayed, 2 nd 3 ' EGF or NGF pulse, which only can induce adaptive ERK activity transients."
26613961
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"The distinctive kinetics of ERK activation induced by light and EGF was clearly resolved by the normalized ratio of nuclear and cytoplasmic ERK2-GFP fluorescence intensity."
24667437
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"This finding suggests that Grb2 is the only adapter involved in the activation of Erk by EGF."
8524249
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"These data indicate that EGF stimulates active ERK in a time- and concentration dependent manner in freshly isolated pGCs and that this experimental approach represents an effective manner with which to evaluate the role of EGF and the ERK signal transduction pathway in freshly harvested pGC."
10503724
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"EGF activated IκB and Erk whereas TNF activated only IκB (Figure  xref C)."
23800251
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"Thus, inhibition of PKHD1 can cause EGF-induced excessive proliferation through decreasing intracellular Ca(2+) resulting in EGF-induced ERK1/2 activation."
17669261
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"Preincubation of cells with U0126, an inhibitor of MEK, the major activator of ERK kinase, completely blocked activation of ERK1/2 by either EGF or NA ( xref )."
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"On the other hand, LL-Z1640-2, a natural compound isolated as an anti-protozoan (Ellestad et al., 1978), has been reported to inhibit anisomycin induced JNK and p38 activation but not epidermal growth[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
11104828
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"Indeed, the EGFR was not efficiently ubiquitylated in cells that overexpressed cortactin, resulting in inhibition of the ligand induced downregulation of the receptor and sustained epidermal growth factor induced Erk activity."
16280034
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"In addition, CI-1033 also inhibited the EGF stimulated activation of ERK in these lines (XREF_FIG)."
18927496
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"In Panc-1 cells, FTS at a concentration of 25-100 microM reduced the amount of Ras in a dose dependent manner and interfered with serum dependent and epidermal growth factor stimulated ERK activation, thus inhibiting both anchorage dependent and anchorage independent growth of Panc-1 cells in vitro."
10353601
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"The initial phase (up to 20 min; unaffected by transcription level effects) of the EGF mediated ERK pathway response in HEK293T or in HEK293T-ΔRSK1/2 cells was used to fit the Network Model (see Fig. 1; blue: ORF45 is not expressed; red: ORF45 expression is turned on)."
35078976
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"While EGF induced ERK1/2 activity was common to both MCF-7 and pII, only the latter showed increased phosphorylation of p38 and Akt, suggesting a greater involvement of these pathways in estrogen desensitized cells."
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"Using a series of inhibitors and an assay for arachidonic acid release, Sauvant et al. concluded that EGF successively activates MEK, ERK1/2, and phospholipase A 2 (PLA 2), leading to an increased rel[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
14729155
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"In addition, the G i2α ribozyme resulted in a partial decrease of the EGF-induced ERK1/2 activation, in accordance with a role of G i ."
11495629
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"Under the same conditions used in the gene expression experiment, 2-APB did not inhibit rapid and robust EGF activation of the ERK and AKT (protein kinase B) signaling pathways."
26551735
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"Band density measurements further confirmed that inhibition of EGFR suppresses EGF induced ERK1/2 and Akt activation in HER2 overexpressing cells (XREF_SUPPLEMENTARY)."
21911055
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"This effect was specific to NKA-mediated signaling, as MB5 failed to inhibit dopamine- and EGF-induced ERK activation ( xref – xref )."
33956349
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"Thus, both EGF and PDGF similarly activate Erk1/2 signaling in 3T3 fibroblasts and MSC."
27110716
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"Because epidermal growth factor can activate both ERK1 / 2 and ERK5 [ 5,23 ] , the remaining U0126-resistant Kv4.2 phosphorylation component may result from ERK5 activity ."
29996472
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"Because a PKC inhibitor Gö6983 did not inhibit EGF-induced ERK activation, we speculated that a PLC-mediated increase in Ca 2+ influx enhanced the Ras-mediated ERK activation in a PKC-independent manner."
20071468
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"As can be seen, both the experimental data and model simulations show that EGF stimulated ERK activation is indeed more sensitive to U0126 than HRG stimulated ERK activation."
18004277
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"NSCs from GD3S-KO mice showed decreased self-renewal ability compared with those from the wild-type animals, and that decreased ability was accompanied by reduced expression of EGF receptor (EGFR) and an increased degradation rate of EGFR and EGF induced ERK signaling."
24198336
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"Inhibition of ERK activity by U0126 treatment significantly prevented EGF-induced cell invasion, suggesting that EGF-induced ERK activation is responsible for the invasion of these cells."
23747719
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"In this review, we will center our discussion on the role and regulation of EGF receptor in LPA signaling.LPA- and EGF induced Erk MAP kinase activation signaling pathways share several similarities."
12069816
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"EGF induced ERK1/2 activation in A549 cells expressing PR-B was significantly reduced in as few as 30 min, while EGF induced ERK1/2 activation in A549 cells expressing PR-BDeltaSH3 showed prolonged ER[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
26892043
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"As morphine had not been previously tested for its long-term effect on EGF activation of ERK, it was examined and found to mimic the DAMGO action ( xref )."
19457093
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"Use of suboptimal EGF concentrations revealed the potentiating action of PTHRP on EGF induced ERK activation more clearly (XREF_FIG)."
12592371
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"Moreover, EGF activation of the Erk1/2 signaling pathway greatly reduces the concentration of estrogen required to induce PI-9 and shield the cells from NK cell mediated cytolysis."
23791814
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"PL inhibited the activation of EGF induced ERK1/2 and Akt kinases and decreased the protein level of c-Fos, which eventually attenuated AP-1-mediated Cyclin D1 expression."
32606774
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"These findings were formalized mathematically [XREF_BIBR, XREF_BIBR] and have now also been validated experimentally by Hornberg et al. [XREF_BIBR], by measuring the time-course of extracellular signal related kinase (ERK) phosphorylation in fibroblast (NRK) cells treated with EGF in the presence or absence of inhibitors of the upstream MAP kinase kinase (MEK) or an inactivating MAP kinase phosphatase (PTP)."
16207364
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"Moreover, relevant studies have shown that EGF can activate ERK signaling pathway, and increase phosphorylated MEK1/2 and ERK1/2, which can regulate the redistribution of TJ proteins ZO-1, occludin an[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
25721793
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"Here, PDGF, FGF2, and EGF treatment of PA-SMCs resulted in rapid ERK1/2 activation, and this effect was completely blocked by suramin without changes in total ERK1/2 levels."
24143201
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"Untransfected AP-1 cells, or AP-1 cells stably expressing WT hNHE1, were exposed to EGF (100ng/ml) to induce ERK1/2 activity."
27083547
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"Using a keratinocyte cell line expressing an inducible form of the Raf kinase, we show that UTP inhibits the EGF-induced ERK1/2 pathway activation downstream of Raf."
22718344
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"Phosphoimmunoblotting of PCMO indicated that both EGF and HB-EGF activated MEK-1/2 and ERK1/2 in a concentration-dependent fashion with the effect of EGF being more prominent [ xref ]."
26798361
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"Thus, epidermal growth factor stimulation of ERK prevented SMAD1 activation by bone morphogenetic proteins in epithelial cells."
17325210
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"The results indicated that RKIP inhibited the EGF activated ERK signaling in HCCLM3 cells."
26116914
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"On the other hand, EGF triggered a brief ERK activation, insufficient to induce nuclear translocation and consequent neuronal differentiation [22, 41, 44, 114]."
19305741
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"These same cells also downregulated elastin mRNA in response to heparin binding epidermal growth factor induced activation of extracellular signal regulated kinase (ERK) [9]."
22796164
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"DeltaNp63alpha potentiated EGF mediated activation of ERK, Akt and JNK signaling."
22053213
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"In particular tumor models, the addition of EGF serves to activate the ras --> raf --> MEK --> ERK cascade as a collateral stimulus to TGF-betaR-dependent signaling."
20204159
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"These findings are consistent with our data that EGF stimulation causes PI3K-independent activation of ERK in BT-474."
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"However, our results revealed that EGF induced ERK1/2 activation was resistant to PI3K inhibitors in BEL-7402 cells, regardless of the EGF concentration, suggesting that the role of PI3K in EGF stimul[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16406609
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"It was found that EGF stimulation actually results in a transient activation of ERK, while NGF elicits a sustained ERK activation, which leads to a neuronal differentiation program."
20373871
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"The results indicated that the steepness of the EGF induced ERK nuclear translocation response was more gradual in Nup153 depleted cells than in non depleted cells (XREF_FIG), suggesting a contribution of Nup153 to regulation of the ERK nuclear translocation response."
26786866
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"Phospho-ERK staining in cancer and adjacent normal area from a single specimen is demonstrated in fig. 4, A and B. EGF Induced activation of extracellular signal regulated kinase and cell proliferatio[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
10492251
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"Furthermore, EGF activated Erk within 5-15 minutes and pre-addition of aspirin inhibited the EGFR activated Erk in OVCAR-3 cells."
24155779
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"The EGF dependent activation of Erk1/2 was unaffected by LY294002 (data not shown)."
15194442
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"To delineate the biochemical mechanism by which II-B08 exerts its growth inhibitory activity, we analyzed the effect of II-B08 on EGF mediated ERK1/2 activation, which plays a critical role in cell proliferation."
24041688
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"Interestingly, after MAPK and ERK activation either by EGF treatment or overexpression of mutated V600E BRAF, the expression of miR-524-5p in HEK293 cells decreased by approximately 2.6-fold."
25275294
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"EGF activated the EGFR, AKT, and ERK1/2 pathways in a time-dependent manner (Fig. 2D)."
35013128
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"This allowed the identification of several hitherto 18 unknown modulators of EGF stimulated ERK signaling."
24994965
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"Agents that cause only transient ERK activation, such as EGF, do not cause neurite outgrowth."
10354710
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"We used a flow cytometry-based phosphorylation assay (FCPA) [ xref ] to determine the kinetics and dose response of ERK activation by EGF in HEK293 cells."
22920937
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"We also demonstrated that while overexpression of the dominant negative form of Ras, N17-Ras, could inhibit ERK activation induced by EGF as reported [27,28]; it could not inhibit its activation induc[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18771726
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"On the other hand, an obligatory tyrosine phosphorylation step for activation of ERK was indicated by the use of protein tyrosine kinase inhibitors, which profoundly inhibited the activation of ERK by EGF, Ang II, and PMA."
9933031
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"Initial insight into this specificity dilemma came from observations that PC-12 cells proliferated after a transient ERK activation by epidermal growth factor (EGF), but differentiated after a sustained ERK activation by nerve growth factor (NGF), showing that the duration of ERK signaling is critical for cell fate decisions ( xref )."
20493519
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"The duration of Epidermal Growth Factor (EGF) stimulation modulates ERK dynamics and controls differentiation."
26488364
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"It has been reported that transient overexpression of IQGAP1 in MCF-7 breast cancer cells significantly reduced activation of ERK1 and ERK2 by EGF, namely phospho-ERK1/2 was decreased. xref , xref Roy et al xref have reported that maximal activation of MEK and ERK by EGF was observed only when cellular IQGAP1 concentrations were close to normal levels."
28223821
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"It is clear that, under the influence of a classic ERK1/2 activator such as EGF and in the absence of mitochondrial PKA activation, the cytosolic and not the mitochondrial MEK1/2 pool is activated."
18197253
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"For example, in foreskin fibroblasts and carcinoma cells, thapsigargin- and ionomycin induced ERK activation was Ca 2+ dependent, whereas EGF induced ERK activation was Ca 2+ independent, suggesting t[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
9933031
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"XREF_BIBR, XREF_BIBR In MCF-10A cells, sustained EGF stimulation was observed to cause oscillations of ERK activity in single cells."
31190430
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"We also observed the effects of EGF on the activation of EGFR, ERK1/2, and STAT-3; the cells were treated with 10 ng/ml EGF for the indicated times."
19385051
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"Although further studies are required to clarify the relationship between EGFR signaling pathways and COX-1 in ovarian cancer, here we show that aspirin blocked EGF stimulated Akt and Erk activation in COX-1 stable transfected cells."
24155779
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"We also investigated a hypothesis resulting from preliminary examination of the experimental data and model simulation results : the differential sensitivity of EGF- and HRG stimulated ERK activity to the MEK inhibitor U0126."
18004277
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"The activation of ERK1/2 by EGF was, however, reduced to a lesser extent."
11495629
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"Inhibition of MEK1 inhibited basal as well as EGF-induced ERK activation and migration."
15496479
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"In monolayer culture, overexpression of Gab2 accelerated EGF induced cell cycle progression and was associated with enhanced and/or more sustained EGF induced ERK and AKT activation."
19737390
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"To investigate the role of protein kinase C (PKC) isozymes in epithelial growth factor (EGF)-induced activation of extracellular signal regulated kinase (ERK) and cell proliferation in cultured human corneal epithelial cells."
12454035
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"The ERK1/2 activator EGF was found to antagonize F 2 inhibition of LDH and cTnI leakage and improvement of cell viability (P < 0.05)."
24392181
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"EGF stimulation of cells is known to activate the Mapk and Akt pathways, and the phosphorylation profiles of the activation loop sites were consistent with the results from Western blotting and KAYAK (XREF_FIG, parts A and B)."
23607489
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"Wild type BRCA1 reportedly inhibits estradiol- as well as EGF induced activation of ERK and cell proliferation, but not the activation of AKT."
26385184
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"Compounds 1 and 4 displayed strong cytotoxicity against three human tumor cell lines with GI50 values in the submicromolar range, whereas 2 showed subnanomolar activity as an inhibitor of EGFR-MAPK-AP1-mediated mitogenic signaling, causing inhibition of EGF mediated AP1 trans-activation and EGF mediated ERK activation and slight inhibition of EGF mediated JNK activation."
21718029
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"Therefore, it may be reasonable to speculate that EGF-induced ERK and Rac1 activation and cell migration require the PH domain of GEP100 and are mediated through the GEP100/Arf6 pathways."
22701712
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"This difference may be because trametinib, like SC-1-151, inhibits both ERK1/2 and ERK5 activation by EGF in MDA-MB-231 cells, but only inhibits the ERK1/2 activation in BT-549, TU-BcX-4IC, and TAMR MCF-7 cells."
33761370
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"However, chronically elevated levels of GH result in desensitization of STAT mediated EGF signal and similar response of ERK1/2 and AKT signaling to EGF compared to normal mice."
28223314
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"In both High Na + and Low Na + buffers, EGF activated EGFR, Erk1/2 and Akt to a similar extend suggesting that activation of canonical EGFR signaling is independent of the EGF-induced sodium influx (Fig.  xref )."
26382850
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"For example, epidermal growth factor (EGF) induces transient ERK activation in PC12 cells, resulting in cell proliferation."
19046323
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"Importantly, both of these siRNAs also markedly reduced EGF-induced ERK1/2 activation but did not decrease LPA-induced ERK activation ( xref E), suggesting that LPA can bypass the effects of down-regulation of AGK."
15939762
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"In contrast to IGF-1, AICAR did not block epidermal growth factor (EGF)-dependent Raf-1 and Erk activation, but our results demonstrated that multiple Raf-1 upstream pathways induced by EGF were differentially affected by AICAR: inhibition of Ras activation and simultaneous induction of Ras-independent Raf activation."
11262401
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"Surprisingly, we found that interaction of Grb2 to SGEF antagonizes the ability of SGEF to enhance EGF-induced ERK1/2 activation."
24399467
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"EGF triggers RET inhibitor resistance by reactivating AKT and ERK signaling."
27873490
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"In previous studies, we compared human and rat goblet cells, and found that they were similar in their stimulation of proliferation by EGF, activation of ERK1/2 by EGF and cholinergic agonists, and the signaling pathways activated by EGF to stimulate proliferation ( xref , xref )."
21357260
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"HGF-stimulated ERK activation increases the Gab1/PI3K association, whereas EGF-stimulated ERK activation results in a decrease in the tyrosine phosphorylation of Gab1 and a decreased association with the PI3K."
11896055
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"To test for possible non specific effects of G36 or G15, we examined EGF induced ERK activation, which was unchanged by the presence of either G15 or G36, illustrating the selectivity of each of these molecules for GPER."
21782022
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"The prediction that ErbB2 overexpression will sustain EGF induced ERK signaling is corroborated by studies of ErbB2 overexpression in HMECs, which showed that at 10 and 30 min after EGF stimulation, ERK activation is between 1.15- and 2-fold higher for cells overexpressing ErbB2."
18004277
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"G i α proteins formed a complex with the EGFR, its adaptor Gab1 and phosphatase Shp2 in response to EGF, and loss of G i α proteins impaired EGF-induced interaction of Gab1 with Shp2, which is crucial for the activation of ERK1/2 by EGF [ xref ]."
24521094
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"The ERK signaling by IL-6 and EGF included several cases that lie outside of the 95% confidence interval."
16271139
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"In addition, in D283 cells, a cell line derived from medulloblastoma metastasis in the peritoneum, ouabain did not inhibit EGF-induced activation of Erk1/2 but reduced EGF-induced Akt activation (Fig. S2A) and almost completely abolished EGF-induced EGFR phosphorylation (Fig. S2B)."
25052069
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"Moreover, we established that ERK1/2 activation by EGF leads to the mobilisation of α6β4 integrin from HD."
22718344
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"It inhibits the EGF mediated ERK kinase activation and cell proliferation in an AT 2 -receptor dependent manner."
22153618
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"We therefore constructed a new model of the EGF activated ERK pathway by taking the original Brown EGF model and expanding it to include receptor production and degradation as well as the C3G/Rap1/B-Raf pathway, which we believed were important processes in both normal EGF and cancerous signalling."
19804630
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"CTTN expression maintained EGF-induced ERK activation."
27903975
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"We now provide evidence that EGF activates Akt and PKB and Erk1/2 in MMECs that overexpress c-Myc, and that both pathways inhibit c-Myc-mediated apoptosis, with Akt providing stronger survival signali[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12837294
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"RCE in passage number 12-20 are a physiologically relevant model for studies on growth factor receptor mediated control of cell cycle progression and differentiation in its parent tissue as each of these phenomena were conserved : 1) EGF induced EGF receptor activation; 2) EGF activated ERK signaling; 3) expression of cornea specific differentiation markers; 4) karyotype profile; and 5) cell cycle control and progression."
12045889
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"Taken together, EGF stimulates Erk1/2; p38 and cPLA2 activity."
11562791
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"In Ca-Ski cells EGF-induced ERK1/2 activation was not affected by SkQ1 in contrast to SiHa cells."
27902484
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"In HEK293 cells, NSC-87877 inhibited EGF stimulated Erk1/2 activation with an EC 50 of 6 muM [XREF_BIBR]."
20337577
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"Further analysis revealed the requirement of CNKSR2 for NGF but not EGF-induced activation of ERK [ xref ]."
35053419
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"To further investigate the bifurcation in EGF mediated activation of ERK1/2 versus STAT3 signaling upon subcellular compartmentalization of gefitinib, MDA-MB-468 cells were exposed to Gef-NArg, Gef-SV40-NArg, or gefitinib in a two-fold series of concentrations up to 1 muM for gefitinib or 5 muM for each peptoid conjugate (XREF_FIG)."
28787571
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"This model provides a possible explanation for our results in WB cells, which demonstrate that the ERK pathway, when activated by EGF or Ang II, showed a similar sensitivity to inhibition by genistein[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
9933031
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"Interestingly, in this study silencing of clathrin heavy chain augmented EGF stimulation of Erk, a result inconsistent with studies using dominant negative dynamin as a way of blocking endocytosis [XREF_BIBR]."
19615955
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"Since our data show that EGF activates Erk1/2 in high-density cells but they do not divide, Erk1/2 activation by itself is not sufficient to lower p27 below the level critical to allow proliferation."
15194442
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"This EGF induced activation of ERKs occurred within 2.5 min of treatment, continued through 10 min of exposure, and was maximal with 10 ng/ml of EGF [13]."
10503724
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"We also demonstrated that while overexpression of the dominant-negative form of Ras, N17-Ras, could inhibit ERK activation induced by EGF as reported ; it could not inhibit its activation induced by P[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18771726
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"Our data showed that the initial EGF mediated activation of the ERK and MAPK pathway is similar in both cell lines."
20642837
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"Importantly, the presence of WT hNHE1 was necessary for detectable activation of ERK1/2 by EGF."
27083547
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"snSPION induced ERK activation is strongly AKT dependent, which resembles the scenario that EGF mediated ERK activation requires PI3K for post-peak activity XREF_BIBR."
23162692
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"In this work we studied the influence of wortmannin, LY294002 and apigenin on the ERK1/2 activation pathway promoted by EGF in PC12 cells, and their effects on neurite outgrowth as compared to that pr[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
11801244
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"EGF treatment resulted in a robust activation of ERK within 5 min, peaking at 20 min (~ 3.7 fold) and persisting (> 2 fold) for up to 180 min."
20671228
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"We found that hNatB knock-down markedly reduced ERK1/2 phosphorylation at 72 h and 96 h after siRNA transfection (Figure xref ) and that EGF-induced ERK1/2 activation was severely blunted in hNatB-deficient cells (Figure xref , xref )."
28498797
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"Thus, as seen in primary monolayer cultures [13] , the EGF-induced activation of ERKs in freshly isolated pGC is sensitive and occurs rapidly."
10503724
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"Shp-1 and -2 also co-operate in epidermal growth factor induced Erk activation, with Shp-2 acting as a scaffold recruiting Shp-1 to the receptor."
21177494
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"Since Shoc2 overexpression increased the duration of EGF induced ERK activation (XREF_FIG), we tested whether Shoc2 overexpression in PC12 cells modified the EGF induced biological fate of these cells."
25514808
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"A volume of 8 μM sorafenib was used to induce cellular senescence, and ERK was activated by 30 ng/ml ERK agonist EGF."
35910032
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"Next, we examined the effect of SF1126 on EGF mediated activation of ERK (phospho-ERK) in these cells."
25425962
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"In contrast, EGF strongly stimulated ERK without reducing its activity upon longer treatment in CNK1 knockdown cells."
27901111
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"Allograft tumors established from Ptch -/- BCC cells also showed activation of Mek and Erk and Jun, similar to Ptch -/- BCC cells treated with EGF in vitro (XREF_SUPPLEMENTARY)."
22294553
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"For example, an MP1, p14, and MEK1 complex is localized to late endosomes, and this localization is required for EGF induced ERK1/2 signaling [XREF_BIBR - XREF_BIBR]."
22776333
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"Epithelial growth factor (EGF) was able to stimulate ERK activation and decreased HCV RNA load while a MAPK-ERK pathway inhibitor U0126 led to an elevated HCV RNA load and higher NS5A protein amounts in Con1 cells."
23001481
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"In this study, we used Luminex multiplexing assays and high-content single-cell imaging to demonstrate that EGF triggers temporal activation of extracellular-signal-regulated kinases 1/2 (ERK1/2) in Hs578T cells."
35122791
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"Soluble EGF induced rapid activation of both EGFR and the downstream target ERK 1/2 with peaks in phosphorylation after 30 and 60 minutes, respectively (XREF_FIG) and a return to near-baseline levels by 4 hr."
19544388
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"XREF_FIG and XREF_FIG) and the ERK and p38MAPK pathways triggered by PDGF-BB, bFGF, and EGF were closely associated with SMC de-differentiation (Figs."
10330402
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"Transfection studies in COS-1 cells demonstrated that in vivo phosphorylation of epidermal growth factor stimulated ERK depended on VHR protein levels."
10224087
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"Conversely, increasing the negative surface charge deactivated the network and locally suppressed chemoattractant-induced protrusions or subverted EGF-induced ERK activation."
36202973
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"Although NS5A inhibits MAPK/ERK activation induced by exogenous EGF, ORF3 was described as an activator of MAPK/ERK pathway like hPIV3-C."
19806178
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"Spry2 has been shown to interact with c-CBL to inhibit EGF mediated ERK and MAPK signaling."
20501643
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"Transfected Gα z did not replace ADP-ribosylated G proteins and couple κ OR-mediated inhibition of EGF activation of ERK (Fig. 3B) ."
10989284
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"Most importantly, all three treatments greatly prolonged the EGF induced activation of ERK1/2 (XREF_FIG), also consistent with the results obtained under continuous EGF conditions."
25510652
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"We also checked whether the EGF signaling-mediated activation of ERK was required for the binding of the two proteins."
31558706
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"This finding suggests that Grb2 is the only adapter involved in the activation of Erk by EGF."
8524249
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"In particular, aspirin decreased phosphorylated Akt and Erk activated by EGF."
24155779
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"In HeLa cells, addition of EGF induces activation of Erk1/2 as expected."
24606918
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"MyD88 (myeloid differentiation primary response gene 88)-independent activation of ERK by epidermal growth factor (EGF) increased p-ERK and c-Myc and restored the multiple intestinal neoplasia (Min) phenotype in Apc (min/+)/Myd88 (-/-) mice."
20473309
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"From the transcriptional analysis it was clear that EGF receptors are actively signaling to ERK1/2 for approximately 60-90 min post-EGF stimulation."
12191474
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"Transient activation of ERK by EGF and sustained activation of ERK by NGF result in proliferation and differentiation, respectively.Finally, I discuss the threshold model which can be viewed either as[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12654253
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"CMECs after being cultured for 2-3 days were randomly allocated to the following groups : control, control + the ROS donor XO/HX (con + XO/HX), control + the ERK1/2 activator EGF (con + EGF), control + the JNK and p38 activator ANISO (con + ANISO), H/R, H/R + the ROS scavenger NAC (H/R + NAC), H/R + the ROS scavenger EDA (H/R + EDA), H/R + ERK1/2 inhibitor U0126 (H/R + U0126), H/R + JNK inhibitor SP600125 (H/R + SP600125), H/R + p38 inhibitor SB203580 (H/R + SB203580), H/R + different doses of F 2, H/R + F 2 + XO/HX, H/R + F 2 + EGF, and H/R + F 2 + ANISO."
28111550
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"Like other growth factors, EGF activates ERKs mainly by engaging the Grb-2-SOS-Ras-Raf-MEK-ERK pathway [ xref ]."
20946955
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"Furthermore, these effects appear to be Src-specific because CD2 expression had no effect on EGF-induced activation of EGF receptor and ERK."
26551526
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"Selective inhibition of the EGF receptor (EGFR) with AG1478 blocked ERK and p38 activation at both concentrations; however, selective inhibition of the ErbB2 receptor (EB2R) with AG825 or small interfering RNA (siRNA) blocked low but not high EGF activation of ERK and p38."
17372305
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"Tethered EGF induced higher levels of activation of EGFR and ERK1/2 on various peptide gel compositions at later time-points compared to soluble EGF."
20304480
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"This implies that EGF induced ERK activity is controlled by different cellular mechanisms at different times; the descending portion (post-peak) but not the rising portion (pre-peak) of the response curve is highly dependent on PI-3K activity."
18004277
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"Fourth, active ERK was primarily observed in the nucleus of cells treated with EGF."
12644307
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"However, EGF-induced ERK activation was not affected by Bainiku-ekisu."
12467906
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"Pretreatment with AG1478, at a concentration (250 nM) that completely blocked EGF induced ERK-1/2 Thr 202 / Tyr 204 phosphorylation in parallel cultures (results not shown), did not prevent NT induced ERK-1/2 Thr 202 / Tyr 204 or c-Jun Ser 63 phosphorylation in PANC-1 clones."
20082306
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"EGF induced ERK activity stimulates FOS transcription, but FOS is rapidly degraded."
20495582
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"Overexpression of EGFR in MCF-7 cells confers estrogen independent growth, and studies using an MCF-7 cell line model of hormone resistance showed that EGF dependent activation of heterodimers of EGFR and HER1 and HER2 resulted in activation of the ERK pathway and increased cell proliferation."
26832794
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"However, a MORG-MP1 association would make available the whole spectrum of substrates both to serum- and to EGF induced ERK signals, provided that trans-phosphorylation occurred between both scaffold complexes."
27303664
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"We show that overexpression of HRAS and other constituents of the RAS-ERK pathway leads to the prolongation of EGF-induced ERK activation and that this is sufficient to transform the EGF signal into a differentiating stimulus."
27099370
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"Furthermore, Sef does not inhibit ERK activation by epidermal growth factor (EGF) or platelet-derived growth factor (PDGF) in NIH3T3 cells, suggesting its function may be restricted to FGFR signaling ( xref )."
24127237
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"Gab1-SHP2 interaction has been shown to mediate ERK (extracellular-signal-regulated kinase) activation by EGF (epidermal growth factor)."
15952937
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"Consistent with the above results, our data revealed that PI3K is involved in insulin stimulated ERK1/2 activation but not EGF stimulated ERK1/2 activation in MCF-7, SMMC-7721 and LNCaP cells."
16406609
reach
"Since 2-APB did not block EGF activation of the ERK pathway, the PLCgamma-IP 3 R mediated increase in intracellular calcium is a previously undescribed independent regulator of EGF induced gene expres[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
27354311
reach
"It was shown that although EGF and NRG can both stimulate ERK1/2, EGF acts primarily through EGFR and ERBB2 heterodimers to preferentially stimulate PKC, whereas NRG acts primarily through ERBB2 and ERBB3 heterodimers to preferentially stimulate AKT [XREF_BIBR]."
28513565
reach
"While quantitative and systems-level analyses of EGF stimulated ERK activity have been performed, these studies have focused on acute re-stimulation of cells with growth factors following a period of withdrawal, which induces ERK signaling within minutes, followed by proliferation many hours later."
23219535
reach
"After the ERKs activation began to decline after peaking, the ERKs were activated again by another stimulus of 25 ng/ml of EGF, confirming re-activation of the ERK by sequential stimulations.In additi[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
29306762
sparser
"To address this question, we used MCF-7 mammary epithelial cells, in which EGF-induced transient ERK activation triggers proliferation, whereas sustained ERK activation in response to treatment with heregulin (HRG) leads to adipocytic-like differentiation ( xref ; xref )."
27099370
reach
"In conclusion these data show that 8PN increases EGF induced Erk 1/2 activity, likely through ER activation, while it does not induce and inhibits the EGF mediated Akt activation, through an unknown m[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
19103290
sparser
"PC-3 and Du145, but not LNCaP, proliferate in response to LPA and S1P. Epidermal growth factor (EGF), phorbol 12-myristate 13-acetate (PMA), LPA, and S1P induce activation of Erks in PC-3 and Du145; only EGF and PMA activate Erks in LNCaP."
19536794
reach
"Our findings supported these data; the ERK1/2 was activated by the EGF stimulation and the active ERK1/2 was immediately localized within the nucleus (Figs. 4 and 5 b)."
22085529
sparser
"For instance, EGF-activated Erk can directly phosphorylate Smad3 at Ser 207, Ser 203, and Thr 178 residues, which suppress Smad transcriptional activity xref ."
29735981
sparser
"In mouse neuroblastoma N1E-115 cells, stimulation of AT2R inhibited EGF-induced ERK1/2 activation and cellular proliferation, and interestingly, not only was this effect blocked by PD123319, but it was also significantly inhibited by ATBP50 siRNA, indicating a functional modulatory interaction between ATBP50 and AT2R-mediated anti-mitogenic signalling (Wruck et al., 2005)."
18804122
sparser
"EGF activation of ERK prior to irradiation was associated with a marked increase in PARP activation and decreased survival in both cell lines."
17295209
sparser
"However, our previous studies indicate that Sef inhibits FGF2 but not PDGF or EGF induced ERK activation in NIH 3T3 cells, and thus the observed effects are likely to be FGF specific."
24127237
reach
"2.7 Low doses of EGF induce a pulsatile response of the ERK pathway."
33319088
reach
"We demonstrate that Shh has the capability to transactivate the EGFR and as a consequence strengthens the mitogenic intensity of the ERK1/2 signaling pathway triggered by EGF."
24133411
sparser
"Overexpression of N -terminal tagged PEBP4 resulted in an inhibition of ERK activation by EGF, whereas C -terminal tagged PEBP4 was without such an effect."
27033522
sparser
"EGF-induced ERK activation is mediated by EGF receptor type 1 while opioid receptor activation seems to brings about stimulation via EGF receptor type."
15464054
reach
"Thus, pharmacologic blunting of basal and EGF induced ERK activity in C14 cells augmented early EGF induced EGFR activation; however, the overall 1068 Y phosphorylation signal at later EGF stimulation time points in PD98059 pretreated cells was not enhanced, likely in part because EGFR abundance was lessened at these later points in the inhibitor pretreated cells."
18762250
reach
"Stimulation with EGF caused ERK activation that was also inhibited by both drugs."
23106397
reach
"While NGF results in a slower but more pronounced and long-lasting activation, EGF activates Erk1/2 earlier but only transiently [30]."
21187008
reach
"Pretreatment with the EGF receptor kinase inhibitor AG1478 had, remarkably, no significant effect on Erk1/2 activation induced by any of these adrenergic agonists (although it fully abolished EGF induced Erk1/2 activation), demonstrating absence of EGF receptor mediated transactivation."
23948306
reach
"In cPLA2alpha overexpressing cells, the basal levels of phosphorylated PKB and AKT and ERK1/2 were increased, and the EGF treatment further increased PKB and AKT and ERK1/2 activation."
28649002
reach
"Epidermal Growth Factor dependent Activation of the Extracellular Signal regulated Kinase Pathway by DJ-1 Protein through Its Direct Binding to c-Raf Protein."
26048984
reach
"We found that hNatB knock-down markedly reduced ERK1/2 phosphorylation at 72 h and 96 h after siRNA transfection and that EGF induced ERK1/2 activation was severely blunted in hNatB deficient cells."
28498797
reach
"Indeed, pre-treatment of BPH-1 and PrEC cells with cetuximab is enough to reduce the amount of ERK signaling induced by Fc-Ecad and EGF (XREF_FIG)."
22024284
sparser
"Similar results were obtained by evaluating ERK1/2 phosphorylation: PP1 prevents both SDF-1α- and EGF-induced ERK1/2 activation (data not shown)."
15921680
reach
"We have determined whether the presence of PR-PPD could change the kinetics of EGF induced ERK1/2 activation."
26892043
reach
"We also showed that loss of SCIN decreased the EGFR protein level and dampened the EGF stimulated MEK and ERK signalling pathway."
29744289
sparser
"Concentrating on PC9ER cells and contrasting EGF and IFN-γ, we verified the kinetic differences and noted that IFN-γ rapidly activated STAT-3, whereas EGF activated ERK ( xref A)."
34038737
reach
"HEK-293 cells endogenously express the EGFR, and EGF produces robust ERK activation concomitant with EGFR tyrosine phosphorylation."
11897012
reach
"Here we show that caspase-8 is also critical for EGF induced activation of the Erk pathway."
19470771
reach
"EGF induced ERK activation promotes CK2 mediated disassociation of alpha-catenin from beta-catenin and transactivation of beta-catenin."
19941816
reach
"Because SHP2 phosphatase activity is required for the full activation of the Ras-Erk1/2 pathway, XREF_BIBR, XREF_BIBR we assessed the cellular effect of 11a-1 on EGF induced Erk1/2 activation in H1975 cells."
25003231
reach
"The group found that EGF transiently activated ERK MAP kinases, whereas PDGF stimulated prolonged activation."
| PMC
reach
"ERK activation is triggered by the activation of epidermal growth factor receptors (EGFRs) through binding to their cognate ligands."
25668746
reach
"It is, thus, reasonable to speculate that somatostatin and SSTR5 signaling may inhibit EGF stimulated ERK kinase activation, leading to down-regulation of PDX-1 expression through a mechanism involved in the regulation of PDX-1 ubiquitination and stability."
25009500
reach
"Our results showed that EGF stimulated both MAPK and ERK and mTORC1 pathways, but had no effect on the PI3K and AKT pathway, suggesting that mTORC1 stimulation in EGF treated cells was regulated primarily by the MAPK (ERK1/2) signaling cascade and was independent of the PI3K and AKT pathway."
28963126
sparser
"In a study by Impagnatiello et al. [ xref ] indicating an anti-proliferative effects of the overexpressed Spry1 and Spry2 on endothelial cells in the presence of FGF, VEGF, and EGF, while FGF- and VEGF-induced activation of ERK were repressed by Sprouty, EGF-activated ERK was left unaffected."
24744103
sparser
"Both MEK inhibitors UO126 and PD184352 completely abolished the activation of ERK by EGF and snSPIONs."
23162692
reach
"Furthermore, we identified the PI3-kinase pathway to be specifically redox regulated as compared to the Erk1/2 pathway, which was activated similarly by PDGF and EGF in a redox-insensitive fashion."
27110716
sparser
"3.3 PR-PPD mediated inhibition of EGF-induced ERK1/2 activation."
26892043
sparser
"But in contrast to NGF/GDNF, there is no EGF induced activation of Erk1/2 detectable in the heterogeneous system of DRG-neurons."
21187008
reach
"Dual specificity phosphatases 10 and 16 are positive regulators of EGF stimulated ERK activity : Indirect regulation of ERK signals by JNK and p38 selective MAPK phosphatases."
22245064
reach
"We found that SB203580 by itself did not affect the activity of ERK1/2 but significantly extended EGF induced ERK activation in PC12 cells, which resulted in early morphological differentiation."
11746502
sparser
"Silencing of hSPRY2 decreased serum- or epidermal growth factor (EGF)-elicited activation of AKT and ERK1/2 and reduced the levels of EGF receptor."
18070883
reach
"Fig. 7 clearly shows that while ERK activation by EGF can occur without participation of PKC and PLD, its activation by PDGF and PMA requires PLD and/or PKC."
12782152
reach
"Co-transfection of cells with dominant negative mutant N17 Ras or with a betagamma scavenger, CD8-betaARK-C, suppressed opioid stimulation of ERK1 and ERK2.When EGF was used to activate ERK, chronic m[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
10989284
sparser
"This study demonstrated that the ubiquitination-dependent down-regulation of the EGFR is the major mechanism for EGF-induced transient ERK activation."
25491268
sparser
"EGF activated ERK in both A549 and H1299 cell lines and the action was more prominent on the transduced population of H1299 cells (Fig.  xref b)."
34045585
reach
"To quantify cell heterogeneity, we pooled all high- and low-dosage, EGF- and NGF triggered ERK activation trajectories described above (Fig XREF_FIG E-H), and used k-means clustering to extract 5 representative temporal activation patterns (Fig XREF_FIG A)."
26613961
sparser
"In COS cells, it has been reported that EGF-induced activation of ERKs is wortmannin-sensitive, but only when very low levels of EGF receptors are activated [15] ."
11341977
reach
"To verify the hypothesis that miR-543 regulate the progression of breast cancer via MAPK signal pathway, the gene expression and protein level of related factors including ERK2 and its phosphorylation site p-ERK1/2 were detected, as well as the pathway downstream factors such as RSK2, MSK1, p-RSK2 and p-MSK1 in the group of miR-543 mimics and MAPK and ERK activated group (miR-543 mimics treated with EGF)."
28331335
reach
"Stable silencing of CIP4 resulted in defects in EGF induced Erk activation, and reduced motility and invasiveness of H1299 cells."
25174397
reach
"The activation of ERK1/2 by EGF was, however, reduced to a lesser extent."
11495629
reach
"Thus, EGF stimulation can not induce ERK signaling activation (XREF_FIG c)."
33319088
sparser
"Unexpectedly, beta-arrestin-2 siRNA diminished both EGF-induced ERK activation and primary astrocyte proliferation suggesting that this adaptor protein plays a novel role in EGF signaling as well as in the opioid receptor phase of this pathway."
19457093
sparser
"Therefore, Fumos inhibits EGF-induced activation of ERK1/2 downstream of Ras in cells expressing wild type Ras."
26626996
reach
"In addition, EGF and FBS rapidly activated ERK signalling in prostate cells (XREF_FIG)."
28256562
sparser
"However, in medulloblastoma cells, ouabain did not transactivate EGFR as has been reported in other cell lines, and ouabain inhibited EGF-induced Erk1/2 and Akt activation (Wolle et al., xref )."
25852478
reach
"Anti-tumor activity of GW572016 : a dual tyrosine kinase inhibitor blocks EGF activation of EGFR and erbB2 and downstream Erk1/2 and AKT pathways."
12214266
reach
"Addition of exogenous epidermal growth factor (EGF) to wounded Pax6 +/- cells restored the calcium wave, increased ERK1/2 activation and restored the immediate healing response to wild-type levels."
16914058
sparser
"Thus, it is possible that NMDA receptor-mediated Ca 2+ influx induces transactivation of EGF, which in turn activates ERK1/2."
19056470
reach
"Treatment with therapeutic anti-EGFR antibody Cetuximab completely blocked EpEX-Fc- and EGF mediated activation of ERK1/2 and AKT (XREF_FIG), demonstrating an EGFR dependency of signaling."
30261040
reach
"EGF could stimulate the proliferation of ectopic endometrial cells by activating the ERK pathway under estrogen deprivation conditions."
24103125
sparser
"All the CPP tested, either against PDGFRβ or VEGFR2, had no inhibitory effect on EGF-induced ERK or Akt activation."
25298192
sparser
"In cells treated with AG1478, activation of ERK1/2 by both EGF and Ang II was eliminated."
18687808
reach
"Recent work in both cell lines [XREF_BIBR] and xenograft models [XREF_BIBR] indicates that the paxilin protein is phosphorylated by either androgen or EGF induced ERK signaling, facilitating signal transduction and resulting in paxillin translocation to the nucleus."
24752388
reach
"In MCF-7 and MDA-MB-231 human breast cancer cell lines, both LPA and EGF stimulated proliferation, Erk activation, Akt activation, and CCN1 induction."
26821052
reach
"Thus, inhibition of PKHD1 can cause EGF induced excessive proliferation through decreasing intracellular Ca (2+) resulting in EGF induced ERK1/2 activation."
17669261
sparser
"Effects of EGF are maximal at 5 minutes and wane by 1 hour; EGF activates ERK and AKT with similar kinetics."
19168439
reach
"Therefore, the effects of the five compounds on FGF23- and EGF-mediated ERK reporter activities were measured."
35868851
sparser
"In addition, clonogenic survival assays revealed that matuzumab and cetuximab reduced the survival rate of H292 cells, in which they also inhibited the EGF-induced activation of Akt and Erk."
18033688
reach
"The JNK and ERK pathways were also activated in cells treated with EGF; however, activation of these pathways was not correlated to the induction of IL-1beta expression."
23383347
reach
"Increased surface charge subverts EGF induced ERK activation."
36202973
sparser
"RES pretreatment attenuated ERK phosphorylation when CFs were stimulated with 0.2 nM epidermal growth factor (EGF), a concentration at which EGF-induced ERK activation over basal was similar to the phosphorylation induced by 100 nM ANG II."
15498824
reach
"Furthermore, picomolar concentrations of EGF activate selectively ERK and PI3K and Akt pathways while PLCgamma, which produces IP3 and triggers Ca 2+ store release, is activated only by nanomolar EGF concentrations XREF_BIBR."
25265278
sparser
"One viral protein, NS5A, which interacts with receptor binding protein 2 (Grb2), inhibits the activation of ERK1/2 by epidermal growth factor (EGF) [19]."
15916886
sparser
"The insertion of this flexible segment results in a similar extent of Erk activation by EGF and TGF-α ( xref , xref ), which is consistent with the importance of this linker in coupling the conformations of the extracellular module and the transmembrane helix."
34846302
reach
"EGF dependent activation of Erk1/2 and Akt pathways may regulate cell cycle progression through control of p27 protein levels or by causing p27 to be sequestered away from its nuclear site of action [[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15194442
sparser
"The pharmacological activation of ERK by epidermal growth factor (EGF) in tanycytes can restore leptin transport and rescue the normal phenotype in obese mice xref ."
35879459
reach
"Consistent with the previous results using RKIP-/- MEFs, EGF stimulated ERK activation remained severely compromised (XREF_FIG)."
23416466
sparser
"EGF-induced ERK activation was shown to correspond with a decreased binding of RKIP to RAF, and therefore a decreased inhibition of ERK signaling ( xref )."
33139506
sparser
"Thus, although PTHRP-induced mitogenesis may occur through an ERK-independent mechanism, the ability of PTHRP to potentiate the activation of ERK by EGF and IGF-1, together with the ability of cells to respond to autocrine PTHRP, could provide one explanation for the increased mitogenic responsiveness and reduced doubling times of MCF-7 cells overexpressing the PTHRP-R."
12592371
sparser
"In contrast, EGF induced ERK1/2, p38 MAPK and Akt activation in MPSC1 cells."
22479527
reach
"However, rapid activation of Erk by EGF did result in significant smad2 linker phosphorylation (XREF_FIG)."
22880011
reach
"The role of MEK in the AhR suppression mechanism has been definitively established by the complimentary experiment in which ERK activation is restored by either CA-MEK1 or EGF."
12758056
sparser
"Re-expression of wild-type PTEN or inhibition of PI 3-kinase caused a recovery of the sensitivity of these cells to IGF1 and insulin, without altering the activation of ERK by EGF and PDGF ( xref )."
17486056
reach
"Hence, the very large CXCR4 overexpression in the SP may provide a SP cellular activation that is synergetic with the EGF initiated ERK activation."
19324848
reach
"Further investigation revealed that both stimuli activated the same protein kinase, the extracellular signal regulated kinase (ERK), with a distinct temporal feature -- i.e., EGF induces a transient ERK activation -- whereas NGF triggers a sustained ERK activation [XREF_BIBR, XREF_BIBR, XREF_BIBR]."
28208785
sparser
"Using a series of inhibitors and an assay for arachidonic acid release, Sauvant et al. concluded that EGF successively activates MEK, ERK1/2, and phospholipase A 2 (PLA 2 ), leading to an increased r[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
14729155
reach
"In OVCAR-3 cells, EGF activated Erk and Akt, but an Erk inhibitor had no impact on cellular viability."
25258648
sparser
"Because Gab1’s association with Shp2 is critical for ERK1/2 activation by EGF and IGF-1 [ xref , xref , xref ], and because Grb2’s association with FRS2 is critical for this activation by bFGF [ xref , xref ], we determined if G i α proteins are important for these associations."
24521094
reach
"As shown in XREF_SUPPLEMENTARY, EGF induced sustained ERK1/2 activation in p53-273H-expressing H1299 cells was largely abolished by EGFR knockdown, indicating that mutant p53-273H promotes sustained EGF induced ERK1/2 activation through EGFR."
23559009
reach
"EGF causes an increase in [Ca 2+] i and activates ERK 1/2 to stimulate mucin secretion, similar to cholinergic agonists."
22975404
sparser
"To investigate this, we used MCF-7 cells, in which EGF-induced transient ERK activation triggers proliferation, whereas sustained ERK activation in response to heregulin leads to adipocytic differentiation."
27099370
reach
"To validate the putative ERK-mediated phosphorylation site in PFAS, we performed tandem mass spectrometry (MS/MS) analyses of PFAS immunopurified from EGF-stimulated cells in the presence or absence of an ERK inhibitor to identify potential ERK-regulated phosphorylation sites."
32485148
reach
"Importantly, inhibition of PDE3, and thus perturbation of the spatiotemporal regulation of PKA activity, dramatically increases the duration of EGF stimulated nuclear ERK activity in a PKA dependent manner."
21807900
reach
"A sos1 (-/-) cell line, which expressed readily detectable levels of the closely related Sos2 protein, formed complexes between Sos2, epidermal growth factor receptor (EGFR) and Shc efficiently, gave normal Ras.GTP and ERK responses when treated with EGF for < or = 10 min and was transformed readily by activated Ras."
10675333
reach
"Since internalization occurs prior to both recycling and degradation, it is easy to speculate how Cbl dysfunction could sustain EGFR activation if a loss of EGFR degradation were to occur.Evidence sup[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
26093903
reach
"Epidermal growth factor (EGF) and nerve growth factor (NGF) both activate extracellular signal regulated kinase (ERK) and p38 mitogen activated protein kinase (MAPK) [XREF_BIBR]."
22203867
trips
"Inhibition of Src tyrosine kinase activity abrogated EGF-stimulated Erk activation and cell migration."
14665621
sparser
"8-Br-cAMP and EGF conjointly increased ERK activation above that of either agonist alone in ADPKD cells, and this combined effect was abolished by PD98059, indicating that ERK was activated by EGF- and cAMP-responsive cascades that converge at MAPK."
12753285
reach
"As shown in Fig. 2 c and d, phosphorylated AKT and ERK were observed in cells treated with EGF."
27639114
reach
"Our results are consistent with a report by Larbouret et al in 2007, which reported similar limited efficacy of HER2 inhibition in blocking EGF mediated activation of ERK and AKT in pancreatic cancer cells."
26262587
sparser
"We reported that mammalian Sproutys selectively inhibit FGF-induced Erk activation but do not inhibit EGF-induced Erk activation [20] ."
12646235
reach
"However, we demonstrated that EGF induced activation of the ERK pathway did not activate the Aurora-A promoter, but indeed participated in increasing the protein expression of Aurora-A in colorectal cancers and cervical cancer cells (XREF_FIG, XREF_FIG and D)."
19799648
reach
"This indicates that 669 T, but not 654 T, is required for EGF induced ERK dependent EGFR PTP101- reactive phosphorylation."
18762250
reach
"For instance, EGFR endocytosis to early endosomes was found to be required for the full stimulation of EGF induced ERK1/2 and PI3K and AKT signaling."
29176966
reach
"As shown in XREF_FIG, a 3.5-fold increase of ERK1/2 phosphorylated Exo70 was detected in cells treated with EGF (see XREF_SUPPLEMENTARY for all the statistical analyses of the binding results shown in XREF_FIG)."
22595671
reach
"However, in human embryonic kidney (HEK293) cells, we could recently demonstrate that attenuation of inhibitory Gi/o signaling by the regulator protein Goloco failed to affect epidermal growth factor (EGF) receptor induced ERK1/2 activation."
| PMC
reach
"Notably, 11a-1 efficaciously attenuates cell viability and blocks EGF induced ERK1/2 activation in H1975 lung cancer cells; abrogates ERK1/2 and AKT activation in SKBR3 breast cancer cells and suppresses cell growth in a 3D Matrigel environment; and inhibits oncogenic KITD814V induced constitutive cell growth."
28855209
reach
"In Csk (-/-) fibroblasts, suppression of SFK results in prolonged EGF induced activation of Erk1/2, with concomitant suppression of EGFR degradation."
16324154
reach
"EGF stimulation increases EGFR tyrosine kinase activity and ERK1/2 activity, resulting in increased ADAM17 mediated cleavage of LAR [XREF_BIBR]."
21235433
reach
"Our data suggest basal AKT phosphorylation and the degree of EGF induced activation of AKT and ERK as molecular determinants of erlotinib efficiency in PC cells."
23180942
reach
"EGF stimulation increased the intensity of ERK1/2 and Akt activation in all cells, but was short lived in the controls and prolonged in the HER2 and the BT474 cells."
21911055
sparser
"Shp-1 and -2 also co-operate in epidermal growth factor-induced Erk activation, with Shp-2 acting as a scaffold recruiting Shp-1 to the receptor ( xref )."
21177494
reach
"In support of this, EGF stimulates Müller glia proliferation in an ERK1/2-dependent manner in retinal explants from juvenile mice (Ueki & Reh 2013)."
32343929
reach
"Because a PKC inhibitor Go6983 did not inhibit EGF induced ERK activation, we speculated that a PLC mediated increase in Ca 2+ influx enhanced the Ras mediated ERK activation in a PKC independent manner."
20071468
trips
"Transfection of GPR30 into MDA-MB-231 cells restores their ability to stimulate adenylyl cyclase and attenuate EGF-induced activation of Erk-1/-2 by estrogen."
11773440
reach
"We found that antiestrogens failed to inhibit ERK activation induced by EGF (data not shown), suggesting that high concentrations of antiestrogens may fail to activate the MAPK and ERK."
22276155
reach
"Mutant p53-273H promotes sustained EGF induced ERK activation via the miR-27a and EGFR axis."
23559009
reach
"The role of protein kinase C (PKC) in the EGF stimulated ERK signaling pathway was further examined by inhibition of PKC with the staurosporine analog, CGP41251, and by down-regulation of PKC via chronic treatment with PMA."
7860643
reach
"Regarding the regulation of AQP-3 expression, a previous report had shown that EGF could trigger the activation of EGFR, PI3K, and ERK signaling pathways to regulate the AQP-3 expression in dermal fibroblasts, which is involved in fibroblast migration [XREF_BIBR]."
28813533
sparser
"Consistent with ERK1/2 activation in pLE cells by EGF, P70RSK and RPS6 proteins were significantly phosphorylated in a time-dependent manner and that effect of EGF was, significantly inhibited by U012[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
26620571
reach
"One of the best characterized responses to EGFR stimulation by EGF is phosphorylation of Extracellular signal regulated protein kinases 1 and 2 (ERK1/2), which we analyzed in endothelial cells untreated or treated with EGF for different times."
33348877
sparser
"AG1478, an inhibitor of EGFR, also attenuated EGF-induced activation of EGFR, JNK2, ERK1/2, and Akt."
25970488
reach
"Thus, in MM1 cells in which sorafenib fails to suppress the EGF dependent activation of MEK and ERK1/2, AZ628 efficiently impaired the signaling cascade from Raf to MEK and, consequently, to ERK1/2."
28545562
reach
"The results showed that the ERK1/2 and AKT pathways were activated by EGF and suppressed by EGFR-TKI."
29691367
reach
"Thus, EGF strongly activated both the ERK1/2 MAP kinase pathway and Akt in IMCD cell suspensions."
18667481
reach
"EGF, which activates ERK and p38MAPK, but not JNK, ameliorates CyA induced nuclear translocation of R-Smad and apoptosis through activation of p38MAPK in RPTCs."
22470391
reach
"34 SCCs exhibit enhanced secretion of several cytokines, such as EGF, which induce ERK activation via a ras dependent signaling mechanism."
16436672
sparser
"Both TNF and exogeneous C6-ceramide interfered with the mitogenic activation of Raf-1 and ERK by epidermal growth factor and down-regulated v-Src-induced Raf-1 kinase activity."
9450998
sparser
"RNAi studies showed that MLK3 promotes microtubule instability, is required for cell proliferation in colon epithelial and lung fibroblast cells, and is required for activation of ERK and SAPK/JNK by TNF and EGF [ xref , xref ]."
19586614
sparser
"The growth factors NGF, GDNF and EGF activate the MAP-kinase Erk1/2 via receptor tyrosine kinase signalling."
21187008
reach
"As a control, we verified that the inhibitors, at the concentrations used, abrogated ERK activation induced by EGF or IGF-1 in MiaPaCa-2 cells (XREF_SUPPLEMENTARY)."
25673820
reach
"EGF activation of the Erk and protein kinase B/Akt pathways is enhanced, both in amplitude and duration, by TBC1D3 expression, whereas RNA interference silencing of TBC1D3 suppresses the activation."
18319245
reach
"To test whether elevated intracellular calcium was stimulating ERK activation (Chao et al., 1992), and activated ERK then induced immediate early gene expression, we examined the effect of 2-APB on ra[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
26551735
reach
"MMP9 expression is known to be stimulated via EGF mediated ERK pathway activation."
28827448
reach
"The epidermal growth factor (EGF) receptor also activates Ras and ERK signaling, but produces proliferation instead of differentiation."
8981234
reach
"EGF induced ERK activity time- and dose-dependently with a maximal effect observed at 10 min and 10 nM, respectively."
11812003
sparser
"Moreover, we found that the EGF-induced activation of the downstream signalling proteins Erk and Akt was also inhibited by 17-AAG ( xref )."
19142186
reach
"The results showed that both GEP100 siRNA and GEP100-^ PH largely inhibited EGF induced ERK activity (XREF_FIG)."
22701712
reach
"These results suggest the direct coupling of GPR4 to the adenylyl cyclase system.Based on the observation that ERK activity induced by S1P or EGF was reduced by the overexpression of GPR4 in HEK293 ce[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17462861
reach
"To investigate whether ECM1 regulates the phosphorylation level of PKM2 via the EGF and ERK pathway, we treated the cells with EGF and U0126, which respectively activate and inhibit ERK activity."
25446258
sparser
"PMA-enhanced, EGF-induced ERK activation amplifies the c-Fos expression response."
20493519
reach
"Pharmacological inhibition of EGFR and ERK1/2 abrogated expression of GLI/EGF target genes, suggesting that EGF can signal via MEK/ERK to cooperate with GLI proteins in regulation of target gene expression [82]."
29695137
reach
"This sequence illustrates how non uniform EGF stimulation, which is visualized in the top row of images, can trigger a traveling wave of activated RAS that spreads over the plasma membrane (XREF_SUPPLEMENTARY) and that produces a transient gradient of ERK activity within the cell, which is visualized in the bottom row of images."
28045041
reach
"One important insight enabled by our study is that EGF solely leads to adaptive ERK activation (in the absence of continuous signaling input), while NGF can generate both adaptive and bistable outputs depending on growth factor input strength and duration and on population signaling heterogeneity."
26613961
reach
"By contrast, wild-type p53 failed to promote EGF induced sustained ERK1/2 activation (XREF_FIG)."
23559009
sparser
"EGF, which activates ERK and p38MAPK, but not JNK, ameliorates CyA-induced nuclear translocation of R-Smad and apoptosis through activation of p38MAPK in RPTCs."
22470391
sparser
"A weak reduction in EGF-induced ERK activation was detected 24h after PDT (p=0.029) (D), but this was also restored to control levels at 48h."
23671927
reach
"WNK1 is a seronine-threonine protein kinase that is involved in the MAPK cascade and in EGF dependent stimulation of ERK1/2 and ERK5 [XREF_BIBR, XREF_BIBR]."
28498874
reach
"The two tyrosine residues (Tyr 627 and Tyr 659) in the carboxyl-terminal region of Gab1 are required for SHP-2 binding to Gab1 and for EGF stimulated ERK activation, in which another study reported Gab1 and SHP -2 interaction was independent of ERK activation XREF_BIBR."
17211494
reach
"SACC metastasis is affected by the PI3K/AKT and MEK/ERK pathways, both of which are initiated by EGF/EGFR."
35410600
reach
"They also show an enhanced response to IGF and EGF, which in turn activates PI3K-AKT and ERK and MAPK signaling pathways, which cross-talk with elevated ER."
24979294
reach
"HLP46 interrupts Gab1-Shp2 association and blocked Shp2 dependent activation of the Ras and ERK signal pathway induced by EGF."
27939989
reach
"The EGF induced activation of Erk1/2 was also apparent in MDCK and control cells but not in MDCK and CIIA-Flag cells (XREF_FIG)."
22042618
reach
"Transfected Galpha z did not replace ADP ribosylated G proteins and couple kappa OR mediated inhibition of EGF activation of ERK."
10989284
sparser
"It was reported that EGF activates ERKs mainly through the Grb-2-SOS-Ras-Raf-MEK-ERK pathway [ xref ]."
23496982
sparser
"Actually, cAMP-increasing agents, forskolin and isoproterenol, inhibited the basal and EGF-induced ERK activation in vector cells ( Fig. 2 B)."
17462861
sparser
"Chronic treatment with micro or kappa opioid agonists (>/=2 h) inhibits EGF-induced ERK activation in opioid receptor overexpressing COS-7 cells."
10989284
sparser
"For example, WNK1 is required for activation of extracellular signal-regulated kinases 5 (ERK5) by epidermal growth factor (EGF) and Mitogen-activated Protein/ERK Kinase Kinases (MEKK2/3) pathway [ xref ], and Wnt/β-catenin signaling [ xref ]."
32131390
sparser
"In the case of EGF-induced activation of ERK, evidence points to the kinase domain of the receptor itself being responsible for the first committed tyrosine phosphorylation step."
9933031
reach
"Moreover, although downregulation of PTPIP51 could n't inhibit the activation of ERK induced by EGF, it could facilitate the deactivation of ERK induced by starvation."
18771726
reach
"Epidermal growth factor (EGF), phorbol 12-myristate 13-acetate (PMA), LPA, and S1P induce activation of Erks in PC-3 and Du145; only EGF and PMA activate Erks in LNCaP."
19536794
reach
"AG-1478 also suppressed both EGF activated Akt and Erk."
25258648
reach
"We demonstrated that, in NCI-N87 cells, the Erk1/2, Akt, and P38 MAPK pathways are activated by EGF treatment."
12618892
sparser
"It is interesting to note that Spred-2•ΔN does not inhibit Erk activation induced by epidermal growth factor or nerve growth factor ( xref )."
14981116
reach
"In PC12 cells, EGF stimulates a rapid but transient activation of ERK, peaking at 5 mins and returning to basal levels at 30 mins, leading to cellular proliferation [XREF_BIBR, XREF_BIBR]."
19292901
sparser
"We investigate the role of GRK2 on epidermal growth factor (EGF) receptor signaling, including EGF-induced extracellular signal-regulated kinase and mitogen-activated protein kinase (ERK/MAPK) activation and EGFR internalization."
16077899
reach
"As one of the nuclear targets of Erk is Elk-1, a transcription factor of the Ets family, FGF- or EGF induced activation of Erk can be monitored by measuring the rate of Elk-1-dependent transcription."
12646235
reach
"IGF-1, EGF, and estradiol induce cancer cell adhesion via IGF1R and activation of ERK1/2 [61]."
32069926
reach
"Further research is required to determine why the regulation of EGF stimulated ERK1/2 differs so dramatically between H-ras MCF10A cells and DLD-1 cells, however."
18771677
sparser
"Although EGF-induced NF-κB activation was defected in CARMA3 or Bcl10 knockdown cells, EGF-induced ERK activation was not affected in these cells ( xref )."
21406399
reach
"Furthermore, Cu-B inhibits MEK 1/2 as well as the constitutive and EGF-induced ERK 1/2 activation, indicating a definitive involvement of MAPK signal transducers in regulating Cu-B-mediated anti-melanoma activity."
35756619
reach
"This phenomenon is similar to transient ERK activation induced by mitogens, such as EGF in PC-3 cells."
24023871
reach
"For example, in rat PC12 cells, sustained epidermal growth factor (EGF) stimulation of ERK signaling was found to induce proliferation, while sustained nerve growth factor (NGF) stimulation resulted in differentiation."
31190430
sparser
"In CC models, NHERF1 knockdown abolished the inhibition of EGF-induced ERK activation."
35223518
sparser
"Concomitant with a decline in the steady-state levels of the receptor, we observed a decrease in EGF-induced activation of Erk1/2 in Tsg101-deficient cells compared to their isogenic controls that express endogenous Tsg101."
22479596
sparser
"Immunoblot analyses of ERK phosphorylation showed that EGF and PMA activated ERK in an n -butanol-independent way in Cl8 cells (Fig. 6, upper panel) suggesting that for th[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12782152
reach
"In contrast, for a relatively high EGF dose of 60pg/ml, even the shortest period of EGF stimulation, 30s, induces a pulse of high ERK activity (XREF_FIG)."
28045041
reach
"In experiments using in-gel kinase assays, MK-678 also inhibited EGF stimulated ERK activity via a pertussis toxin sensitive pathway, and this effect resulted in inhibition of Elk-1 transcriptional activity."
9142901
reach
"We have published previously that GIT1 is required for PLCgamma and ERK1/2 activation induced by AngII and EGF XREF_BIBR, XREF_BIBR."
23430614
sparser
"EGF-dependent ERK and Akt activation were assessed in untransfected MCF-7 cells or those expressing wild-type or mutant EGFR."
24691054
reach
"Of note, EGF induced activation of ERK was shown to be insufficient to activate iNOS, suggesting pools of betaArrestin- and EGF activated ERK are distinct, or betaArrestin2 recruits additional mediators involved in the B1R regulated iNOS activation."
21397660
sparser
"The results indicated that both EGF and HB-EGF activated MEK and ERK and that the effect was concentration-dependent and more prominent in EGF-treated than in HB-EGF-treated PCMOs (Figure xref C)."
22873932
sparser
"The overexpressed, constitutively active mutant of Gα z (Q205L) inhibited EGF activation of ERK to a comparable extent in the absence and presence of DAMGO (Fig. 5) ."
10989284
reach
"XREF_BIBR These observations are in agreement with previous reports conducted both invivo and invitro that have indicated that BMP-4 inhibits EGF stimulated ERK activation in isolated parietal cells XREF_BIBR and that prolonged overexpression of growth factors in the stomach of mice alters the normal architecture of the gastric mucosa, leading to increased ERK activation, loss of parietal cells, and foveolar hyperplasia."
28462376
reach
"NGF stimulation of PC12 cells results in prolonged activation of both Ras and ERK activity, while EGF causes a transient rise and fall in Ras and ERK activities."
9281586
sparser
"We have previously shown that EGF triggers a robust P-ERK-response in chicken Müller cells by 10 min [ xref ] and the result showed that AG1478 inhibited this EGF-induced ERK1/2 activation in primary chicken Müller cells ( xref )."
27930693
sparser
"To test whether PI3K differentially regulates insulin- and EGF-induced ERK1/2 activation in other cell types, we similarly pretreated human breast cancer MCF-7 cells, hepatocellular carcinoma SMMC-772[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16406609
reach
"Noteworthy, in PC12 cells, EGF induces only transient activation of Erk1/2 and acts as a mitogen without inducing neurite outgrowth [40]."
16168623
sparser
"EGF-induced activation of EGFR, Ras, extracellular-signal-regulated kinase and S6K with or without inhibitors was measured by FRET imaging, and analyzed by semi-automatic image processing."
24848561
sparser
"In keratinocytes, ERK1/2 activated by EGF stabilizes Gli proteins, particularly Gli2."
30463396
reach
"EGF induces the cell viability, migration, invasion and the activation of mTORC2-AKT and ERK signaling pathway in T24 and E6 cells."
24023933
sparser
"To test this hypothesis, EGF-dependent Erk1/2 activation was examined."
15194442
sparser
"Importantly, Gab1FF blocked paxillin-SHP2 complex formation, Src Tyr-530 dephosphorylation, Erk activation, and cell migration induced by EGF."
14665621
sparser
"Phosphoimmunoblotting of PCMOs indicated that both EGF and HB-EGF activated MEK-1/2 and ERK1/2 in a concentration-dependent fashion with the effect of EGF being more prominent."
22873932
reach
"In epidermal growth factor (EGF)-treated cells, tyrosine phosphorylated Gab1 selectively associates with the wild-type but not the mutant Shp-2 molecule, correlating with a defect in EGF stimulated Erk activation 33."
11136824
sparser
"Fumos also inhibits tumor cell proliferation, attenuates Shp2-dependent Ras-ERK1/2 activation induced by EGF, and reduces invasion of HeLa cells and MDA-MB-231 cells by down-regulating Src signaling pathway."
26626996
reach
"In conclusion, EGF acting through its receptor increased [Ca 2+] i and ERK 1/2 activated leading to an increase in mucin secretion in cultured goblet cells."
22975404
reach
"EGF may partially stimulate Erk1/2 activity at supra-physiological levels, but this was not sufficient to stimulate cellular growth."
21699731
reach
"These waves, although not yet fully characterized, are dependent on EGF receptor ligands because inhibition of EGF receptors completely blocks ERK activity propagation [XREF_BIBR], which in turn suppresses collective cell migration [XREF_BIBR]."
30836891
reach
"RWPE1 cells are grown in media containing recombinant epidermal growth factor (EGF), which activates RAS and ERK signaling, but because the RAS and ERK pathway is intact, negative feedback loops can keep ERK activity low [XREF_BIBR]."
25885538
reach
"The cultures treated with both U0126 and EGF had low levels of P-ERK similar to the endogenous levels in control cultures treated with neither U0126 nor EGF."
12921749
sparser
"The importance of PKCzeta in EGF-induced ERK activation can also be shown in several HNSCC and breast carcinoma cell lines as well as in PKCzeta-deficient mouse embryonic fibroblasts."
20407013
reach
"Taken together, the extract of C. sudachi peel may attenuate EGF induced ERK activation through inhibition of not only EGFR but also its downstream molecule (s), such as Ras and Raf-1, or other signaling molecule (s)."
33096942
reach
"Accordingly, we did not observe significant differences in EGF induced ERK or AKT activation between pBabe, WTATG3, and KR cells (XREF_FIG)."
25686249
sparser
"EGF can increase phosphorylation level of ERK independently from KRAS status. xref , xref We found that EGF treatment in DLD1 cells activated ERK and increased the steady-state expression of ILF3 without affecting its mRNA levels (Fig.  xref )."
31772275
sparser
"Fig. 7 summarises the different pathways for ERK activation by EGF, PMA, and PDGF-BB based on the data presented here."
12782152
reach
"AnxA2 antibody inhibits the EGF induced ERK pathway."
25321192
reach
"EGF and HB-EGF activate EGFR, ERBB4, AKT and extracellular signal regulated kinase 1/2 in EGFR (+) primary trophoblasts; however, they do not show an effect on HLA-G (+) EVTs."
25740878
sparser
"To investigate the RAF–MEK–ERK effectors pathway downstream of RasGTP, we examined ERK phosphorylation and noted that both cell lines exhibit EGF-induced activation of ERK kinases ( xref )."
26005835
reach
"Blockade of the epidermal growth factor (EGF) receptor by AG1478 significantly reduces activation of ERK, suggesting that EGF receptor transactivation could act upstream of the ERK pathway in PC12 cells."
20025629
reach
"Of note, EGF and HGF rescued ERK signaling demonstrating that these cells do respond to those ligands, but that they are unable to rescue PI3K-AKT signaling."
21775521
reach
"Overcoming this shortfall, we established a quantitative automated microscopy (QuAM) for a defined and multiparametric analysis of adherent heterogeneous primary neurons on a single cell base.The growth factors NGF, GDNF and EGF activate the MAP-kinase Erk1/2 via receptor tyrosine kinase signalling."
21187008
sparser
"The Erk inhibitor PD98059 blocked the EGF-activated Erk as anticipated, whereas neither SB203580 nor SP600125 significantly inhibited EGF-activated Erk (when normalized to expression levels of Erk as a loading control) (figure xref D)."
25258648
reach
"Thus, in this model the major DUSPs shaping acute EGF mediated ERK responses are in fact JNK and p38 specific MKPs, acting by modulating cross-talk between MAPK cascades, rather than by direct effects on ERK dephosphorylation or scaffolding."
22245064
reach
"EGF and HRG, growth factor ligands for the EGF and ErbB3/4 receptors, induce transient and sustained ERK activity associated with cellular proliferation and differentiation associated with lipid accumulation of MCF-7 cells, respectively [XREF_BIBR]."
25491268
reach
"At the molecular level, IQGAP3 interacts with ERK1 and promotes EGF induced activation of ERK, which in turn appears to be closely associated with its tumor promoting activity."
24849319
reach
"In the MCF-7 breast cancer cell line, resveratrol was found to inhibit EGF induced EMT via inhibition of the EGF mediated Erk pathway activation [XREF_BIBR]."
27231938
sparser
"A volume of 8 μM sorafenib was used to induce cellular senescence, and ERK was activated by 30 ng/ml ERK agonist EGF."
35910032
reach
"As expected, EGF and PDBu both caused rapid and transient ERK activation, increasing ppERK to the maxima at 5-15 min (XREF_FIG C)."
23754287
sparser
"The ALIX-supported MVB sorting has been demonstrated to be a critical determinant in the highly transient nature (10 min) of EGF-induced ERK1/2 activation [ xref ], which reflects rapid silencing of activated EGFR before its degradation."
27462417
reach
"PMA enhanced, EGF induced ERK activation amplifies the c-Fos expression response."
20493519
sparser
"Wild type BRCA1 reportedly inhibits estradiol- as well as EGF-induced activation of ERK and cell proliferation, but not the activation of AKT ( xref )."
26385184
reach
"Under the same conditions used in the gene expression experiment, 2-APB did not inhibit rapid and robust EGF activation of the ERK and AKT (protein kinase B) signaling pathways (XREF_FIG)."
26551735
reach
"By contrast, the allosteric SHP2 inhibitor SHP099 effectively suppressed PDGF- or EGF stimulated ERK MAPK pathway in Swiss 3T3 cells or HEK293T cells, respectively."
30186742
reach
"In PC12 cells, EGF stimulates a transient activation of ERK whereas NGF stimulates a sustained activation of ERK, but it is still currently unclear how these different ERK signal dynamics can be robustly controlled by different upstream receptors utilising the same pathway."
19804630
sparser
"Here, we report that two structurally distinct PI3K inhibitors, wortmannin and LY294002, inhibited insulin-induced activation of ERK1/2 but had no effect on EGF-induced activation of ERK1/2 in hepatocellular carcinoma BEL-7402 and SMMC-7721 cells, breast cancer MCF-7 cells, and prostate cancer LNCaP cells."
16406609
sparser
"In this study, we show that G i α proteins are also important for ERK1/2 activation by EGF."
24521094
reach
"We also found that EGF mediated ERK itself is sensitive to PP2 being reduced by 45% (data not shown)."
19254952
reach
"The MEK inhibitor, PD 098059 inhibited EGF stimulated proliferation and ERK activity in a parallel, dose dependent manner showing that ERK activation is at least permissive for the proliferative response to EGF."
10383890
reach
"Allograft tumours established from Ptch -/- BCC cells also showed activation of Mek and Erk and Jun, similar to Ptch -/- BCC cells treated with EGF in vitro (Supporting Information XREF_SUPPLEMENTARY)."
22294553
reach
"In this study, we used computational modelling techniques to investigate what effects various cancerous alterations had on signalling through the EGF activated ERK pathway."
19804630
reach
"Expression of either SHP2 binding defective Gab1 or Gab2 mutant blocked EGF induced ERK activation."
15952937
reach
"These results suggest that EGF activates the ERK and Akt and NF-kappaB signaling pathways in HNSCC."
25797258
sparser
"Here we identify RSK phosphorylation sites on SOS1 that negatively regulate EGF-dependent ERK1/2 activation, potentially as they create 14-3-3 binding sites that could interfere with molecular interactions of Ras pathway components."
22827337
reach
"EGF mediated ERK activation has also been shown to phosphorylate caspase-9 and inactivate its caspase activity that is also similar to AKT function (Allan et al."
15110179
sparser
"As a control we studied EGF-induced ERK1/2 activation in the primary chicken Müller cells."
27930693
reach
"Our data indicate that H, D or HD mutations on uPAR do not affect EGF induced ERK phosphorylation (XREF_FIG), suggesting that uPAR-integrin interaction is not required for EGF mediated ERK activation."
18940913
reach
"These compelling results lead us to conclude that GH facilitates the synergistic lessening of KSR serine 392 phosphorylation, suggesting that the mobilization of this important EGFR scaffold is an important part of GH 's sensitizing effect on EGF induced ERK activation."
20946955
reach
"In both physiological and cell culture systems, EGF stimulated ERK activity occurs in discrete pulses within individual cells."
26304118
reach
"To explore the role of RKIP in the modulation of EGF stimulated ERK signaling in cervical cancer, the transfected cells were stimulated with EGF, and EGFR and ERK phosphorylation levels were assessed by western blot."
23527098
sparser
"This promotion of EGF-induced Erk1/2 activation is independent of the proteolytic activity of caspase-8 and can be recapitulated using only the pro-domains of the protein."
19470771
sparser
"This mutation (replacement of the linker by GGSGGS) breaks the differential Erk activation by EGF and TGF-α ( xref , xref )."
34846302
sparser
"By contrast, Ca 2+ -inhibited ERK activation by EGF in keratinocytes ( xref ) and chelation of intracellular Ca 2+ in fibroblasts led to an increase in ERK activation ( xref , xref )."
33766558
reach
"Because epidermal growth factor can activate both ERK1/2 and ERK5 [5,23], the remaining U0126-resistant K 4.2 phosphorylation component may result from ERK5 activity."
29996472
reach
"This suggests that HRG stimulates excess MEK activation above what is needed for peak ERK activation, and leads us to hypothesize that HRG induced ERK activity should be less sensitive to a MEK inhibitor than EGF induced ERK activity."
18004277
sparser
"This result suggests that mutant p53 may promote EGF-induced sustained ERK1/2 activation via the miR-27a/EGFR axis."
23559009
reach
"In PC12 cells, epidermal growth factor (EGF) induces transient ERK activation that leads to cell proliferation, whereas nerve growth factor (NGF) promotes sustained ERK activation and cell differentiation."
32817373
reach
"We found that NLRR1 positively regulated cell proliferation through activation of extracellular signal regulated kinase mediated by EGF and IGF-1."
22815527
reach
"Although the binding of EGF to the epidermal growth factor receptor (EGFR) was decreased in GnT-III transfectants to a level of about 60% of control cells, the EGF induced activation of extracellular signal regulated kinase (ERK) in GnT-III transfectants was enhanced to approximately 1.4-fold that of the control cells."
11134020
sparser
"We previously found that the HCV nonstructural 5A (NS5A) protein interacts with growth factor receptor-binding protein 2 (Grb2) adaptor protein and inhibits the activation of extracellular signal-regulated kinases 1 and 2 (ERK1/2) by epidermal growth factor (EGF)."
12186904
reach
"XREF_BIBR - XREF_BIBR Accordingly, the LS mutants are more efficient in dephosphorylating Paxillin and pY118, XREF_BIBR - XREF_BIBR a physiological substrate of SHP2 required for EGF stimulated ERK1/2 activation."
28541680
sparser
"A further study shows that Cdc2 inhibits EGF-induced Erk activation in M phase [ xref ]."
| PMC
sparser
"The loss of epidermal growth factor-induced activation and phosphorylation of ERK1/2 but not of their direct activator MEK1 in HeLa cells transfected with the p38alpha activator MKK6(E) indicated that activated p38alpha may sequester ERK1/2 and sterically block their phosphorylation by MEK1."
11238443
reach
"For example, NGF induces sustained activation of extracellular signal regulated kinase (ERK) and promotes differentiation of PC12 cells, whereas epidermal growth factor (EGF) induces transient ERK activation and provokes cell proliferation."
21849472
reach
"This was initially demonstrated in rat pheochromocytoma PC-12 cells, in which transient ERK activation, stimulated by epidermal growth factor (EGF), resulted in proliferation, whereas sustained ERK activation, evoked by nerve growth factor (NGF), induced cell differentiation."
27099370
reach
"In agreement with these findings, our results showed that EGF induced a time dependent increase in ERK activity."
23747719
reach
"It was reported that EGF-induced ERK1/2 activation occurs mainly through EGFR activation in porcine renal cell (Dong et al., 2004)."
34093234
reach
"XREF_BIBR Similar findings have been reported in MCF7 cells in which moderate doses of luteolin abolished EGF induced increased pEGFR, Akt, and ERK kinase activity, as well as the transcription factor STAT3."
29928143
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"Tyr1068 is a major autophosphorylation site that, in its phosphorylated state, recruits the adaptor protein Grb2, the main step in EGF dependent induction of the Ras-MAPkinase and ERK and Akt and PKB [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
22732145
sparser
"Besides, 20 μM Go6983 abolished EGF-induced ERK activation (Fig. xref ), which further indicated that PKC was the upstream kinase of MAPK pathway involved in the PN-1 up-regulation."
31501409
sparser
"Conversely, others have reported that EGF-induced ERK activation in glioblastoma cell lines leads to phosphorylation of casein kinase-II (CSNK2) and to disruption of the interaction between β-catenin and α-catenin ( xref )."
22234612
sparser
"These findings indicate that Fumos inhibits EGF-induced ERK1/2 activation."
26626996
sparser
"EGF-induced activation of ERK1/2 has been shown to be highly transient in HEK293 cells [ xref ]."
25510652
reach
"In PTX treated cells, mu agonist inhibited EGF activation of ERK by ~ 40%, in the presence or absence of Galpha z transfection."
10989284
reach
"EGF is a mitogen and can activate ERK1/2 [XREF_BIBR, XREF_BIBR]."
21241664
reach
"However, in other studies we have shown that B1R activation does not transactivate the EGF receptor and that EGF mediated ERK activation does not lead to activation of iNOS derived NO production (F.K. Kuhr, Y. Zhang, V. Brovkovych and R. A. Skidgel, unpublished)."
20015194
reach
"Indeed, while there is strong pressure to report cellular responses to EGF in EGFR expressing cells, also other studies show EGFR expression [59,60] in adult DRG but fail to detect EGF induced Erk1/2 signalling activity in cell culture."
21187008
sparser
"Partial inhibition of EGF-induced Raf-1 and ERK activation in the absence of PKC indicates that mechanisms other than PKC also account for Raf-1 and ERK activation in response to EGF in bovine luteal [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12644307
reach
"Cells have to place a value on incoming signals (e.g. EGF induced ERK activity) such that they are either answered by a biological response (e.g. the execution of a transcriptional program) or disrega[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12049770
reach
"A further study shows that Cdc2 inhibits EGF-induced Erk activation in M phase [169]."
| PMC
sparser
"Here we show that treatment of MCF-7 breast cancer cells with EGF activated Akt and ERK, induced morphological changes, and increased cell motility."
17172846
reach
"The ERK1/2 activator EGF was found to antagonize F 2 's inhibition of cTnI leakage and improvement of cell viability (P < 0.05)."
24392181
reach
"None of the five compounds exhibits EGFR tyrosine kinase inhibition and disrupts EGF/EGFR interaction and EGF-mediated ERK activation (Figure 5c)."
35868851
reach
"Intriguingly, several studies have also demonstrated that mammalian Sprouty proteins can increase EGF mediated Erk and MAPK signaling in a cell type dependent manner."
19662191
reach
"In contrast to the enhanced ERK1/2 activation by the full-length galectin-3 and EGF in the MUC1 positive cells, introduction of C-terminal galectin-3 form (galectin-3C) with EGF showed no further effect on ERK-1/2 phosphorylation in comparison to treatment with EGF."
28731466
reach
"These data suggest that EGFRvIII uses a signaling pathway for ERK activation that is different from the pathway used by normal EGF receptor activated by EGF.One possible explanation for this result co[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
11341977
sparser
"However, our results showed that the inhibition of PI3K activity did not alter the activations of ERK and JNK by EGF, which suggests that the activations of ERK and JNK may be differentially regulated[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
21872446
reach
"Further, we present experimental evidence demonstrating that HPV2a E5, similar to HPV16 E5, is able to modulate EGF mediated erk1/2 MAP kinase activation and to down-regulate the expression of MHC class I molecules at the plasma membrane."
14554085
sparser
"By inhibiting the EGF-induced activation of AKT and Erk, both mAbs inhibit lung tumor growth in vivo [ xref , xref ]."
21749705
reach
"Positive rate of ER-alpha36 was increased in high-stage (P =.03) and high-grade (P =.224) endometrial cancer; expression of ER-alpha36 and EGFR exhibited a significant positive correlation (r = 0.334, P =.025) and they showed substantial colocalization on the plasma membrane of glandular cells; phospho-extracellular signal regulated kinase positive rate in ER-alpha36 positive group and EGFR positive group was higher than that of ER-alpha36 negative group (P =.014) and EGFR negative group (P =.016); finally, ER-alpha36 mediated epidermal growth factor stimulated extracellular signal regulated kinase activation in Hec1A cells."
21684519
reach
"This suggests that HRG may stimulate more MEK activity than is necessary to induce the observed peak ERK activation, and led us to hypothesize that HRG stimulated ERK activation would be less sensitive to a MEK inhibitor (U0126) than would EGF stimulated ERK activation."
18004277
reach
"MLK1-4 activated the MEK and ERK pathway in the presence of the RAF inhibitor but not the MEK inhibitor, indicating MLKs directly phosphorylate MEK (XREF_FIG and XREF_SUPPLEMENTARY in H157 cells; note both L779450 and U0126 blocked EGF stimulated ERK activation in cells, XREF_SUPPLEMENTARY)."
24849047
reach
"In contrast, EGF stimulation in PC12 cells triggers cytoplasmic ERK activity only, resulting in cellular proliferation, whereas neural growth factor (NGF) causes nuclear localization, which is essential for neuronal differentiation."
23790495
sparser
"This and previous data lead to the conclusion that EGF successively activates MEK, ERK1/2, and PLA(2), leading to an increased release of arachidonic acid."
12138128
reach
"We previously showed that ERK 1/2 (also known as p42 and p44 MAPK) was activated by muscarinic, alpha 1D -adrenergic, and EGF receptors in the lacrimal gland."
19608535
sparser
"The tyrphostin AG1478, known to inhibit EGF receptor phosphorylation, also inhibited the angiotensin II and EGF-induced activation of ERK, the phosphorylation of the EGF receptor, and the subsequent association of Shc and Grb2."
10446212
reach
"EGF activated ERK1/2 in S2 tubules and IHKE-hOAT1, and, in both cases, inhibition of ERK activation (by U-0126) abolished this stimulation."
14644751
sparser
"A number of studies have provided mathematical models of ERK activation by EGF ( xref ; xref ; xref ; xref ), including the dynamics of transient and sustained ERK activation ( xref ; xref )."
22735336
sparser
"Sustained ERK activation by epidermal growth factor in PC12 cells overexpressing the epidermal growth factor receptor resulted in cell differentiation rather than proliferation, indicating that alteration of ERK activity can induce reversible changes in cell fate progression xref ."
26884720
sparser
"Thus, it was demonstrated that the ubiquitination-dependent downregulation of the EGFR is the major mechanism for EGF-induced transient ERK activation."
25491268
reach
"This effect originated from the decreased activation of cell-growth-related signaling, such as AKT and Erk mediated by epidermal growth factor (EGF), while overexpression of HE4 resulted in enhanced cell growth and suppressed the anti-tumorigenic activity of paclitaxel."
27862665
reach
"Under normal conditions, EGF stimulates a rapid but transient activation of ERK as the signal is rapidly shutdown, whereas under cancerous conditions, the ERK signal can not be shutdown and is sustained."
18681747
reach
"Epidermal growth factor (EGF) and NGF both activate ERK and p38 in PC12 cells, but only NGF trigger differentiation."
11746502
reach
"In contrast, none of these inhibitors adversely affect EGF stimulated Erk activity in breast cells [44]."
11897506
reach
"Thus, weak EGF stimuli and IL-6 might activate Erk1/2 through similar mechanisms."
31651330
reach
"It profoundly inhibited the EGF induced activation of ERK, as expected, but it also inhibited almost entirely the activation of ERK induced by Ang II."
9933031
reach
"EGF increases the TER of MDCK monolayers by inducing ERK1/2 dependent downregulation of claudin-2 (CLDN-2) and upregulation of claudin-4 (CLDN-4)."
24909426
reach
"This has been well documented in the pheochromocytoma cell line PC12, where transient activation of ERKs by epidermal growth factor (EGF) triggers proliferation, whereas sustained activation of ERKs b[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12074885
reach
"Surprisingly, we found that interaction of Grb2 to SGEF antagonizes the ability of SGEF to enhance EGF induced ERK1/2 activation."
24399467
reach
"These findings revealed that the expression of CCL3 in BMMs was regulated by ERK/CREB pathway.Recent one study indicated the EGF can be efficiently produced in MC-38 cells and EGF could activate the ERK pathway [15,16], thus we next explored whether MC-38-derived EGF contributed to the overexpression of CCL3 through ERK pathway."
32478376
sparser
"In addition, the exogenous administration of EGF confirmed the requirement of ILK and IQGAP1 in EGF-induced ERK1/2 activation, NF-κB activation, cell growth, and migration [ xref , xref ]."
25398317
sparser
"To explore the role of active Ras in the insulin- and EGF-induced activation of ERK1/2, we utilized a dominant negative N17-Ras mutant in which the GDP conformation of Ras is stabilized and endogenous[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16406609
sparser
"EGF also induced activation within 2 min, which peaked at 10 min; however, the ERK activation by EGF was sustained at least up to 12 h of exposure to EGF, the longest time tested."
12069834
reach
"While the EGF initiated signaling usually results in a transient extracellular-signal-regulated kinase (ERK) activity in normal fibroblast and epithelial cells, a more sustained ERK signal of sufficient duration is required to pass the R-point [XREF_BIBR]."
28133623
trips
"Inhibitors of EGF-stimulated ERK1/2 activity did not inhibit AKT activation but blocked proliferation."
23882690
reach
"Because glutamine is required for EGF stimulated proliferation and stimulates ERKs in intestinal cell culture, we hypothesized that glutamine and the EGF related peptide transforming growth factor-alpha (TGF-alpha) would synergistically enhance repair associated with stimulation of ERKs."
10026753
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"Furthermore, EGF increased ER stress independent ERK and AKT signaling upon irradiation of OSCC cells."
30414263
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"Restoring Src activity in Shp2 -/- cells by retroviral mediated overexpression of WT Src (to levels that presumably titrate endogenous Csk) or by depleting Csk using siRNA increased EGF stimulated Erk[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
14967142
sparser
"As a result, this compound inhibits growth-factor-dependent activation of MEK1 and ERK in intact cells and proliferation of ras- or raf-transformed rodent fibroblasts in anchorage-independent growth a[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
9561854
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"Working with MCF-10A mammary epithelial cells, which require EGF stimulated ERK signaling for proliferation, we assembled a panel of live-cell and high-content immunofluorescence (HCIF) measurements for ERK pathway signaling and proliferation (XREF_FIG)."
23219535
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"Since MEKs are upstream kinases which phosphorylate and activate ERKs, we also observed the down-regulation of EGF-induced ERK activation in GnT-III transfected B16-F1 melanoma cells."
10571019
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"These results suggest that EGF induced ERK1/2 activation is independent of Src."
16414009
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"As expected, PD 098059, a specific inhibitor for ERK1/2 activation by inhibiting MAPK kinase, abolished nicotinic acid-, UTP-, and bradykinin-promoted ERK1/2 activation and inhibited 100 ng/mL EGF-ind[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16674924
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"Thus, low-density cells seem to require both EGF-dependent Erk1/2 and Akt activation to decrease p27 levels sufficiently to allow division."
15194442
reach
"Congruently, PD98059 increased the turnover rate of CSN6 in the presence of the de novo protein synthesis inhibitor cycloheximide, whereas EGF, which induced ERK activation, reduced CSN6 turnover."
26267535
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"The functionality of this interaction was demonstrated by the inhibition of the epidermal growth factor dependent activation of the Erk pathway when PMCA4b and RASSF1 were co-expressed."
15145946
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"EGF was shown to increase time-dependently the activation of extracellular signal regulated kinase (ERK) and c-Jun amino terminal kinase (JNK) in A431 cells [79]."
12432922
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"Overexpression of EGFR1 or HER2 as well as mutations that undermine the negative feedback regulation of the EGFR-ERK signaling pathway may transform the transient mode of the EGF induced ERK signaling to the sustained mode, and uncontrollably drive cells past the R-point [XREF_BIBR]."
28133623
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"Data presented in this manuscript demonstrate that EGF, a potent mitogen for prostate cancer cells, activates ERK and induces cellular proliferation."
10492251
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"Interestingly, even though the cell cycle is profoundly altered by inhibiting CME, inhibition of CME by knocking out dynamin in MEFs does not significantly affect Akt and ERK mitogenic signaling stimulated by EGF."
24138026
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"Still, EGF effectively activated the Erk1/2 pathway and mitotic activity in redox independent fashion."
27110716
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"Knockdown of Hck or Blk reduced EGF-induced ERK1/2, p38, or AKT phosphorylation (Figure 5GA, 5HA) in HaCaT cells, as well as inhibited the phosphorylation of ERK1/2, p38, and AKT in A431 cells (Figure 5GB, 5HB)."
26910280
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"However, knockdown of G i1 α, G i2 α and G 3 α (Gi1/2/3) largely impaired this phosphorylation by EGF (Figure  xref A) indicating that all three G i α proteins are important for the activation of Akt, mTORC1 and ERK1/2 by EGF."
24521094
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"We found that in this case the system can distinguish between sequences ' 10 ' and ' 11 ', because the second EGF pulse increases the ERK activity tail."
30836891
sparser
"Although, PP2 had no effect on EGF-induced ERK1/2 activation [ xref ], it completely prevented EGF-induced migration suggesting that Src activity is essential for migration and that MEK/ERK may regulate downstream processes activated by Src."
22227249
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"During C. elegans vulval development, an EGF signal from the anchor cell initiates the activation of Ras LET-60> Raf LIN-45> MEK MEK-2> ERK MPK-1 signaling cascade to induce the 1degrees cell."
33076222
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"EGFR tyrosine kinase activity is required for both EGF stimulation of ERK and for mitogenic synergism."
12069834
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"EGF induced ERK activation is mediated by EGF receptor type 1 while opioid receptor activation seems to brings about stimulation via EGF receptor type."
15464054
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"Thus, it was demonstrated that the ubiquitination dependent downregulation of the EGFR is the major mechanism for EGF induced transient ERK activation."
25491268
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"Several lines of evidence support the involvement of caspase-8 in EGF signaling pathways inducing ERK activation through the incorporation of caspase-8 in SRC containing complexes."
23070001
reach
"Because both ERK and JNK are potently activated by EGF in MDA-MB-468 cells, and ERK is involved in cell migration, invasion, and EMT, we speculated that hyperactive JNK might modulate ERK activation."
20145041
sparser
"In contrast, in ES2 ovarian cancer cells, which bear a BRAF V600E mutation with VEPH1 amplification but low ERBB2 expression, loss of VEPH1 expression enabled further activation of ERK1/2 by EGF and enhanced EGF activation of AKT."
36828346
sparser
"In addition, LTD(4) did not induce phosphorylation of the EGF receptor, nor did pertussis toxin (PTX) block EGF-induced activation of Erk-1/2, thus refuting a possible crosstalk between the receptors."
11956320
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"A classical example of this can be found in the MAP kinase pathway where transient ERK activation by EGF is associated with cell proliferation while persistent ERK activation by NGF leads to cell differentiation."
21613981
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"CTTN expression maintained EGF induced ERK activation."
27903975
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"Furthermore, ERK inhibitor PD98059 or PI3K inhibitor LY294002 significantly reversed the effects of Si-MIF on PVM/Ms from young mice, whereas ERK activator EGF or PI3K activator IGF significantly reversed the effects of Ad-MIF on PVM/Ms from aged mice."
28694172
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"53 It has been demonstrated that the PI3K and Akt and Erk1/2 signaling pathways are activated by EGF and p38 mitogen activated protein kinase (MAPK) by enhanced oxidative stress, all of which induce migration and invasion mediated by the up-regulation of Snail, Slug, ZEB1, and beta-catenin in association with GSK3beta inhibition in various cancers."
23851495
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"The ERK pathway inhibitor suppressed HRG- or EGF induced ERK * [XREF_BIBR]."
28542548
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"Neutralizing antibody against alpha5beta1 integrin partially (approximately 60%) blocked BK induced ERK activation but did not affect EGF induced ERK activation."
20385709
trips
"Inhibition of Cdc2 activity with roscovitine in mitotic cells restored ERK activation by EGF and PMA."
15888452
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"2) EGF stimulation resulted in increased serine and threonine phosphorylation of nuclear symplekin, which was abrogated by the MEK and ERK inhibitor U0126, indicating that ERK1/2 regulated symplekin phosphorylation can be induced by EGF treatment."
28630428
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"WT-delta-OR-expressing HEK-293 cells were exposed to increasing concentrations of either DSLET (1-1000 nM) or EGF (0.1-100 ng/ml) for five minutes to induce ERK activation."
11897012
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"The EGF induced Erk1/2 activation was enhanced by expression of TBC1D3, but not TBC1D3 mutants deficient in beta-tubulin interacting (XREF_FIG)."
24714105
reach
"Since 2-APB did not block EGF activation of the ERK pathway, the PLCgamma-IP 3 R mediated increase in intracellular calcium is a previously undescribed independent regulator of EGF induced gene expression that works together with ERK activation to regulate immediate early gene expression."
27354311
sparser
"Interestingly trastuzumab strongly inhibits EGF-induced Akt phosphorylation and slightly inhibits EGF-induced Erk activation, in both BT474 and SKBR3 cells."
36552857
sparser
"These results suggest that EGF-induced ERK1/2 activation is independent of Src."
16414009
sparser
"13,62 Blocking of phosphatidylinositol-3-kinase activity with InsP 6 profoundly impaired JB6 cell transformation and ERK activation induced by EGF or TPA, as well as AP1 ac[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
11905657
reach
"In the presence of a NADH and NADPH oxidase inhibitor, diphenyleneiodonium (DPI) or an antioxidant, alpha-tocopherol, Ang II induced protein tyrosine phosphorylation of two major proteins (p120, p70) and ERK activation were markedly reduced, whereas ERK activation by epidermal growth factor was unaffected."
10965882
sparser
"It is noteworthy that NSC-87877 inhibited epidermal growth factor (EGF)-induced activation of Shp2 PTP, Ras, and Erk1/2 in cell cultures but did not block EGF-induced Gab1 tyrosine phosphorylation or Gab1-Shp2 association."
16717135
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"It has been reported that the activation of ERK1/2 by EGF and EGFR is implicated in the transcription of E-cadherin repressors, such as Snail and Slug [XREF_BIBR, XREF_BIBR, XREF_BIBR]."
27829223
reach
"EGF triggered both Akt and ERK phosphorylation in HCT116 cells and induced ERK activation in SW480 cells (XREF_FIG), indicating that KRAS mutation does n't fully take over the ligand mediated ERK activation and also impling the significance of EGFR in KRAS mutant cells."
21464950
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"Ectopic expression GEP100 siRNA, GEP100-^ PH, or Arf6-T27N suppressed EGF induced ERK and Rac1 activity."
22701712
reach
"In the second example [XREF_BIBR], we therefore propose a therapeutic index (TI) function and apply it to a 3D NSCLC model [XREF_BIBR] where, at the molecular level, both EGF and TGFbeta can trigger downstream ERK signaling through different routes."
21572568
trips
"Expression of either SHP2-binding defective Gab1 or Gab2 mutant blocked EGF-induced ERK activation."
15952937
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"In the presence of E2, EGF also induced activation of ERK1/2, and the amplitude of the ERK1/2 activation was similar (P> 0.05) to that observed in the absence of E2."
26620571
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"Binding of the epidermal growth factor (EGF) ligand to EGFR induces homo- or hetrodimerization of the receptor and activation of the kinase domain, ultimately leading to intracellular signaling events, including activation of protein kinase B (AKT), extracellular signal regulated kinase (ERK), and p38 mitogen activated protein kinase (MAPK)."
28978320
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"Exploiting MRA, one study XREF_BIBR found that distinct temporal profiles of active ERK stimulated by EGF and NGF emerge from differential feedback wiring of the ERK cascade, with EGF eliciting negative feedback and NGF inducing positive feedback."
20495582
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"In fact, ERK activation by EGF appeared even to be stimulated upon PKC inhibition (XREF_FIG I)."
25421240
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"Nevertheless, compound 6 is not specific to MEK5 since it also blocks ERK1/2 activation induced by EGF as well."
21385929
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"Conversely, increased ERK1/2 activity, by adding purified active ERK1/2 or EGF induced activation of ERK1/2, significantly reduced gamma-secretase activity, demonstrating down-regulation of gamma-secretase activity by ERK1/2."
16293708
sparser
"EGF activated IκB, Akt and Erk whereas TNF activated only IκB and reduced EGF-activated Akt and Erk in these cells (Figure  xref C)."
23800251
sparser
"Both insulin and EGF activated extracellular regulated kinase 2 (ERK-2), p70rsk and protein kinase B (PKB) and inactivated glycogen synthase kinase-3 (GSK-3)."
9498625
sparser
"In these classical experiments, PC12 cells proliferated after transient ERK activation by EGF, but differentiated after more sustained ERK activation by the nerve growth factor."
19357636
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"Since neither treatment affected the Ang II- or EGF induced activation of ERK, we can conclude that the transient Ca 2+ increase was not obligatory for the early activation of ERK.The fact that neithe[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
9933031
sparser
"As showed inFigure xref , EGF activated ERK in a concentration dependent manner, and it exerted the best effect at a concentration of 100 ng for 1 h (Figures xref ; n = 4, P < 0.05)."
28428752
sparser
"However EGF activated Erk without IκB phosphorylation whereas TNF highly activated IκB and had a small effect on the activation of Erk (Figure  xref C)."
23800251
reach
"Moreover, GPER was required for epidermal growth factor receptor (EGFR) and ERK activation by EGF as ascertained by using MIBE and performing gene silencing experiments."
22251451
sparser
"In contrast, EGF-induced ERK activation is independent of CNK2 expression."
15028221
sparser
"Alone, EGF activated ERK1/2, increased and sustained levels of c-jun mRNA, but had almost no effect on JNK1 activation."
9925763
reach
"The role of endocytosis in ERK1/2 activation by EGF has been previously demonstrated using general inhibitors of CME, although other studies using the same inhibitors produced conflicting results with regard to the role of endocytosis in ERK activity."
20513767
sparser
"We found that treatment of cells with 10 µM U0126 for 2 h prior to stimulation completely inhibited EGF-induced activation of ERK1/2 ( xref )."
22761867
reach
"This study demonstrated that the ubiquitination dependent down-regulation of the EGFR is the major mechanism for EGF induced transient ERK activation."
25491268
reach
"Together, these results reveal that Gab1 recruits SHP2 to dephosphorylate paxillin, leading to dissociation of Csk from the paxillin and Src complex and Src activation and that Src is an SHP2 effector involved in EGF stimulated Erk activation and cell migration."
14665621
reach
"Epidermal growth factor (EGF) caused rapid induction of both JNK- and ERK signaling pathways, and the downmodulation of the EGF signaling pathway by EGF pre-treatment inhibited the UVB induced JNK1 activation."
9182816
sparser
"TNF-α and exogenous C6 ceramide interfere with the activation of Raf-1 and ERK by EGF and down-regulate v-Src-induced Raf-1 kinase activity ( xref )."
23833655
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"EGF also induced robust ERK activation in primary porcine mesenchymal stem cells."
16150920
sparser
"At the molecular level, IQGAP3 interacts with ERK1 and promotes EGF-induced activation of ERK, which in turn appears to be closely associated with its tumor-promoting activity."
24849319
sparser
"Further, G i α proteins can differentially regulate the activation of Akt, mTORC1 and ERK1/2 by EGF, bFGF and IGF-1 in breast cancer cells."
24521094
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"Epidermal growth factor receptors (EGFRs) have been shown to be polarized at the leading edge of migrating epithelial cells in the presence of an electric field and asymmetrically activated downstream MAP kinase ERK1/2 XREF_BIBR."
26898606
sparser
"EGF-induced ERK1/2 activation in A549 cells expressing PR-B was significantly reduced in as few as 30 min, while EGF-induced ERK1/2 activation in A549 cells expressing PR-BΔSH3 showed prolonged ERK1/2[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
26892043
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"EGF mediated induction of survivin requires the activity of Raf-1 and MEK and ERK, but EGF has no significant effect on survivin transcription."
23955284
reach
"Indeed, Cav-1 is a known regulator of the ERK1/2 signalling system [XREF_BIBR, XREF_BIBR] and is involved in EGF mediated ERK1/2 activation [XREF_BIBR]."
25774517
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"Here we also report that ethanol inhibits activation of ERK induced by both EGF and PDGF."
11048648
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"Taken together, these data indicate that PI3K is involved in insulin stimulated ERK1/2 activation but not in EGF stimulated ERK1/2 activation.To test whether PI3K differentially regulates insulin- and[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16406609
reach
"Our results suggest that C23 plays a crucial role in activation of EGF induced ERK and PI3K-AKT pathways via interacting with EGFR; furthermore, C23 could be indicative of an important factor in glioblastoma development and a useful target for glioblastoma treatment."
25015231
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"In contrast, co-administration of EGF and TGF-beta1 caused an early pronounced and persistent ERK1/2 activation."
17467690
reach
"Importantly, SUMO1 modification at K 590 of hShp2 increased Shp2 binding to the Gab1, although having no effect on its catalytic activity, promoting EGF stimulated ERK activation and increasing HCC cell anchorage independent growth and xenograft tumor growth, which reveals a new mechanism for the regulation of Shp2 function in Ras and ERK signaling pathway."
25823821
reach
"Similar to DSLET, EGF (5 ng/ml) produces a robust activation of ERKs in delta-OR-expressing HEK-293 cells after a 5 minute treatment."
11897012
reach
"Alone, EGF activated ERK1/2, increased and sustained levels of c-jun mRNA, but had almost no effect on JNK1 activation."
9925763
sparser
"Gab1-SHP2 interaction has been shown to mediate ERK (extracellular-signal-regulated kinase) activation by EGF (epidermal growth factor)."
15952937
sparser
"Our previous results showed that, in PC12 cells, EGF activated both ERK1/2 and ERK5 while bFGF only activated ERK1/2 xref , xref ."
28724963
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"Here, we show that in medulloblastoma cells, both EGF and ouabain activate Erk1/2 and PI3K and Akt signaling."
25052069
reach
"It would appear that, unlike cultures with soluble EGF on collagen I or peptide gels, DNA synthesis is stimulated via tethered EGF activating EGFR and ERK1/2 without altering levels of EGFR or ErbB2."
20304480
sparser
"Paradoxically, beta1 integrin stimulation increased EGF-induced Erk activation while increasing expression of the inhibitory p66ShcA isoform."
16517240
reach
"Consistent with reports showing that EGFR signaling is coupled to activation of cap dependent translation in EGFR wild type cells, EGF supplementation was able to restore defective Akt and Erk activation in KPn organoids (XREF_SUPPLEMENTARY)."
27477511
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"Sos1 and Sos2 have similar molecular structures, and both proteins mediate EGF induced Erk activation."
19429099
reach
"It should be noted here that in our study EGF induced ERK activation declined with age to a greater extent than did EGF receptor expression or activation, suggesting that additional factors also influence changes in hepatic ERK activity during aging."
18693222
sparser
"The pH-dependent cAMP accumulation may mediate the ligand-independent inhibition of ERK activation, as evidenced by the inhibition of EGF-induced ERK activation by cAMP-increasing agents ( Fig. 2 B)."
17462861
reach
"We compared the time-course of EGF stimulated ERK in the presence of MKP-7 in COS-7 cells."
20122898
sparser
"This observation may contradict the previous report negating the role of PLCγ1 in EGF-induced ERK activation in PLCγ1-deficient mouse embryonic fibroblasts ( xref )."
20071468
reach
"This result suggested that despite the presence of RET inhibitors, EGF promoted resistance by reactivating AKT and ERK signaling."
27873490
reach
"Experiments with HeLa cell lines have shown that DGKeta may act as an adaptor protein during EGF mediated ERK1/2 activation, 150 raising the possibility that DGK isoforms may serve in a similar capacity in T cells."
23582058
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"Activation was maintained through the 10 ng/ml dose of the growth factor.In the last series of experiments, we investigated the time course for EGF activation of ERK in the freshly isolated pGC."
10503724
reach
"At all three levels of Odin expression (basal, ectopic over-expression, knock-down) EGF stimulated ERK activation was achieved by 1 min, sustained at 10 min, and decreasing by 30 min (XREF_FIG)."
23825523
reach
"Of note and at variance with LPA, EGF does not cause plaque dissolution (XREF_FIG) and induces AKT and ERK activation and membrane ruffling independently of N-WASP (XREF_FIG) XREF_BIBR."
28703125
sparser
"Both insulin- and EGF-induced ERK1/2 activation are strictly dependent on Ras activation, however, wortmannin only inhibited insulin-induced, but not EGF-induced Ras activation."
16406609
sparser
"EGF is a mitogen and can activate ERK1/2 [ xref , xref ]."
21241664
sparser
"ERK activation by both LPA and EGF presumably involves the Ras-regulated kinase cascade in these as in other cells [30] , although the involvement of Ras and each of the downstream kinases has not be[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12069834
sparser
"As presented in , EGF induced Erk-1/2 activation in renal interstitial fibroblast NRK-49F cells ()."
12875981
reach
"We also observed that MECs with alpha5beta1 integrin ligated by a wild-type, but not a synergy-site mutated FN, nucleated more vinculin-talin-actin-zyxin scaffold and recruited more PIP3 to amplify EGF dependent activation of Akt and ERK."
28877984
reach
"When transrepressional control is impaired (as in the svp, tll double mutant clones) the threshold is lowered and more cells than appropriate interpret EGF induced ERK activity as a consolidated signa[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12049770
reach
"In addition, NGF stimulation of ERK is known to involve an early transient phase of activation followed by a late sustained phase of activation; in contrast, epidermal growth factor (EGF) elicits only early transient ERK activation."
11161724
sparser
"We have validated the assay on endogenously expressed ERK1/2 activated by the epidermal growth factor as a prototypical RTK, as well as various GPCRs belonging to different classes and coupling to different heterotrimeric G proteins."
25002860
sparser
"As can be seen in xref , II-B08 could attenuate EGF-induced ERK1/2 activation in H1975 cells treated with scrambled siRNA, but the ability of II-B08 to inhibit EGF-mediated ERK1/2 activation was blunted when SHP2 was knocked down with siRNA."
24041688
sparser
"The fact that neither PKC activation nor Ca mobilization could account for the observed ERK activation induced by Ang II and EGF in WB cells indicates that other pathways predominate."
9933031
reach
"3.2 GH augmentation of EGF induced ERK activation in 3T3-F442A cells resides at the Raf-MEK level."
20946955
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"PI3K is required for insulin stimulated but not EGF stimulated ERK1/2 activation."
16406609
reach
"We examined this issue by quantifying the dynamics of Erk signaling induced by epidermal growth factor, basic fibroblast growth factor (bFGF), and platelet derived growth factor (PDGF) in NIH-3T3 fibroblasts."
17540764
reach
"Sprouty evidently inhibits c-Cbl-induced ubiquitination and degradation of EGFR, thereby sustaining EGF activated ERK [XREF_BIBR, XREF_BIBR, XREF_BIBR, XREF_BIBR, XREF_BIBR]."
24744103
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"Otherwise, a recent study described a regulatory signalling pathway of OSCC metastasis consisting of miR-491-5p and its downstream target, GIT1, which functions as a scaffold for EGF induced ERK activation."
25299770
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"MAPK/ERK pathway was inhibited in cells treated with BPH, and DNA damage can be reversed while EGF was added to activate MAPK/ERK pathway."
36572327
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"For example, ERK1/2 and PKB and Akt, components activated upon insulin stimulation, can be activated by hepatic growth factor and epidermal growth factor treatments in rat primary hepatocytes."
24373238
sparser
"These results demonstrate that HRAS is competent for prolonging EGF-induced ERK activation, and subsequently MCF-7 differentiation, only if its signals emanate from plasma membrane DM microdomains."
27099370
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"PPARalpha inhibitor GW6471 and PPARalpha activator Fenofibrate treatments abrogated the effects induced by IGF-1 and PE in cardiomyocytes respectively, as well as ERK1/2 activator EGF and ERK1/2 inhibitor PD98059."
25636810
reach
"Long-term, EGF induced ERK signaling is mainly controlled by ERK receptor feedback (XREF_FIG) and ligand induced degradation (XREF_FIG), short- and long-term HRG induced signaling is mainly controlled by RasGAP phosphorylation (XREF_FIG) and to a lesser extent the ERK-SOS feedback (XREF_FIG) and ERK-Gab feedback (XREF_FIG)."
18004277
reach
"The motogenic effect of TFF2 was previously demonstrated to depend on ERK1/2 and protein kinase C activation; whereas the EGF triggered motogenic response was completely independent of ERK1/2 activation but sensitive to the inhibition of phosphoinositide 3-kinase, p38, protein kinase C, or nuclear factor kappaB."
15256384
reach
"Stable knock-down (KD) of CIP4 in lung adenocarcinoma H1299 cells, expressing wild-type EGFR, led to increased EGFR levels on the cell surface, and defects in sustained activation of Erk kinase in H1299 cells treated with EGF."
25174397
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"In addition, EGF activation of the Erk pathway was also impaired by Src kinase inhibitors PP1 or SU6656 (XREF_FIG, lower panels)."
19470771
sparser
"Therefore, we investigated whether the p38 MAPK and/or PI3K/Akt pathways affect EGF-induced ERK or JNK activations in human skin fibroblasts."
21872446
sparser
"Additionally, EGF-induced PI3K/Akt and ERK activation was inhibited by propolin C in EGFR wild-type A549 lung cancer cells ( xref )."
29681982
reach
"XREF_BIBR showed that 184A1 HMECs with high ErbB2 expression had more sustained EGF induced ERK activity compared to ERK responses from the same cell line with low ErbB2 expression."
18004277
reach
"In contrast, ERK activation by EGF in both cell types was not sensitive to n-butanol."
12782152
sparser
"Based on our knowledge concerning the intimate links between signaling pathways involved in regulating proliferation and oxidative stress responsiveness, and prior findings indicating that EGF-induced[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12213598
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"As activation of ERK increases its nuclear localization, we next investigated whether exposure to EGF enhanced P-ERK and LC3-II colocalization in the nucleus."
24240988
reach
"Furthermore, the observations that EGF stimulation significantly increased protein expression of pAKT and pERK, but not pSTAT3, indicated that both AKT and ERK pathways in sarcomas may be mainly stimulated by EGF and EGFR activation."
26909593
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"Stimulation of EGF receptor couples with the activation of PI 3-kinase in various cells [32], and EGF stimulates ERK in GH3 cells [33]."
10807952
reach
"EGF (50 ng/ml) is added to stimulate the ERK pathway, and the cells are incubated for an additional 5 min."
20811979
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"For example, hepatocyte growth factor or EGF stimulated human epidermal keratinocytes require extracellular signal regulated kinase (ERK), but not JNK-AP-1, activation to stimulate cell molitlity XREF_BIBR."
26846883
reach
"The importance of PKCzeta in EGF induced ERK activation can also be shown in several HNSCC and breast carcinoma cell lines as well as in PKCzeta deficient mouse embryonic fibroblasts."
20407013
sparser
"ERK1/2 activation by PDGF-BB and EGF, however, does not appear to be directly associated with hBMMSC proliferation and is probably involved in other physiological processes induced by these GFs."
18583177
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"In addition, TGFalpha and EGF have been reported to transiently activate ERK1/2, but this transient activation is not sufficient to induce beta-cell proliferation."
25999530
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"Also, EGF induced plasma membrane ERK activation, and cell morphology changes involve Rap1 [58]."
33624208
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"It is evident, therefore, that during the first hour of ERK activation by EGF and Ang II, PKC plays a minor role in the overall effects of the hormones on ERK activation.Since Ca 2+ has been implicate[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
9933031
reach
"Sorting of EGFR onto the ILVs of MVBs is not functionally impaired, as shown by the normal time course of EGF induced ERK1/2 activation and the presence of immunogold labelled EGFR on the ILVs of abnormal endosomes in patient fibroblast cells."
20223751
reach
"Hence, we presume that EGF induced activation of ERK1/2 in primary hBMMSCs is not proliferation related but rather mediates other physiological processes in these cells.Importantly, inhibition of the [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18583177
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"Moreover, UDCA was also able to reduce EGF induced ERK1/2 activity, an observation enhanced in cells stably transfected with caveolin-1."
25027205
sparser
"EGF activated ERK in both A549 and H1299 cell lines and the action was more prominent on the transduced population of H1299 cells (Fig. 4b)."
34045585
reach
"Studies in T47D breast cancer cells have shown that EGF treatment induces Erk dependent PRB phosphorylation on serine residues [XREF_BIBR]."
21258428
sparser
"The overexpression or reduction of IQGAP1 expression by siRNA both caused the activation of Erk2 by EGF or IGF-1 in MCF-7 cells to be reduced, probably due to an optimal level of intracellular IQGAP1 being necessary for maximal level of activation of Erk by EGF or IGF-1 [ xref ]."
17284314
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"In both High Na + and Low Na + buffers, EGF activated EGFR, Erk1/2 and Akt to a similar extend suggesting that activation of canonical EGFR signaling is independent of the EGF induced sodium influx."
26382850
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"However, we demonstrate, for the first time, that EGF dependent activation of ERK, and AKT signaling cascades are insufficient to drive cancer cell proliferation in the absence of SPRY2."
26293890
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"We showed that EGF-induced Raf-1 and ERK activation was reduced (≈60%) in luteal cells rendered PKC-deficient by chronic PMA treatment."
12644307
reach
"This effect was selective since Barbadin did not block EGF stimulated ERK1/2 (XREF_FIG)."
28416805
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"In the case of EGF induced activation of ERK, evidence points to the kinase domain of the receptor itself being responsible for the first committed tyrosine phosphorylation step."
9933031
reach
"These results reveal that Gab2 plays a pivotal role in the EGF induced ERK activation pathway and that it can complement the function of Gab1 in the EGF signalling pathway."
15952937
sparser
"Expression of either SHP2-binding defective Gab1 or Gab2 mutant blocked EGF-induced ERK activation."
15952937
sparser
"As a consequence, EGF-induced activation of Erk and Akt as well as cell proliferation were enhanced in PACSIN2-depleted cells."
23129763
reach
"We recently showed that ouabain inhibits EGF induced Erk1/2 and Akt activation and prevents EGF induced formation of actin stress fibers and cell motility in medulloblastoma cells [XREF_BIBR]."
26382850
sparser
"In contrast, the present study demonstrates that in WB cells, short-term activation of ERK by Ang II or EGF was essentially Ca independent."
9933031
reach
"These EGF ligands then act on the intestinal stem cells (ISCs) to stimulate the Ras and ERK pathway, which triggers stem cell growth and division, and promotes regeneration of the intestinal epithelium."
29401457
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"On the contrary, in TRAF2 overexpressing NP460 cells, EGF stimulated activation of ERK1/2 and its downstream transcriptional factors were substantially enhanced (XREF_FIG)."
32566659
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"Furthermore, these results together with the fact that the MAPK and ERK pathway was activated by EGF in this system suggest that this region is independent of MAPK and ERK activity."
15642133
sparser
"Moreover, BAPTA-AM did not suppress EGF-induced ERK1/2 activation ( xref )."
27086143
reach
"All the CPP tested, either against PDGFRbeta or VEGFR2, had no inhibitory effect on EGF induced ERK or Akt activation."
25298192
reach
"Interestingly trastuzumab strongly inhibits EGF-induced Akt phosphorylation and slightly inhibits EGF-induced Erk activation, in both BT474 and SKBR3 cells."
36552857
reach
"The signaling pathways leading to extracellular signal regulated kinase (ERK) activation by EGF and amphiregulin in the NHBE and BSMC were also assessed (Fig 3, E)."
19895983
reach
"Although treatment of Caov-3 ovarian cancer cells with sorafenib blocked the expression of mesenchymal phenotypes following EGF stimulation, EGF activated Caov-3 cells exhibited reduced MAPK and ERK signaling."
28627617
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"ALIX knockdown cells behaved similarly as the Y319F FLAG-ALIX Bro1 expressing or the HA-ALIX V expressing cells in sustaining the EGF induced ERK1/2 activation (XREF_FIG)."
25510652
reach
"Our data demonstrate that either PM or EN activation of EGFR is sufficient to activate ERK, which suggests that EGFR endocytosis is not required for the activation of ERK by EGF."
22984397
reach
"Nevertheless, siRNAs against two p38 and JNK MKPs (DUSPs 10 and 16) inhibited acute EGF stimulated ERK activation."
22245064
reach
"EGF and TNFalpha ligands stimulate ERK, JNK and p38 MAPK cascades, the PI3K and AKT pathways, and the NFkappaB cascade."
24321545
sparser
"To explore the importance of the PY motif and putative ERK phosphorylation site in the acute downregulation of ENaC by EGF-induced ERK1/2 activation, a stable mammalian expression system was developed."
17724164
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"We observed that GH and EGF each acutely activated ERK, Akt, and phospholipase C-gamma (PLC-gamma) in preadipocytes."
20946955
reach
"The results also suggest that Klotho may inhibit EGF induced ERK1/2 activation independent of p38 MAPK action."
29928482
reach
"More recently, epidermal growth factor (EGF) has been shown to induce ERK activation pulses, the frequency of which depends on the concentration of EGF and controls the velocity of cell proliferation [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
24140422
reach
"Thus, EGF induced a rapid and transient ERK activation that resulted in cell proliferation, whereas NGF induced sustained MAP kinases activation associated with cell differentiation."
12747941
sparser
"Finally, we observed that ERK inhibitor pretreatment alone enhanced EGF-dependent EGFR downregulation [ xref ], strongly suggesting that ERK-mediated EGFR threonine phosphorylation, whether accomplished by PRL or GH before EGF stimulation or resulting from EGF-induced ERK activation, can modulate EGF-induced EGFR downregulation."
18762250
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"We show that overexpression of HRAS and other constituents of the RAS-ERK pathway leads to the prolongation of EGF induced ERK activation and that this is sufficient to transform the EGF signal into a differentiating stimulus."
27099370
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"In these experiments, transient ERK activation by epidermal growth factor (EGF) induced cell proliferation, whereas sustained ERK activation by nerve growth factor (NGF) induced differentiation XREF_BIBR."
20495582
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"The significance of signal propagation through the late endosomes is supported by the recent finding that the localization of the MP-1 and MAPK scaffold to late endosomes via p14 is required for full [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17320394
sparser
"This differential activation of ERK1/2 by NGF or EGF translates into distinct biological responses: NGF promotes PC12 cell differentiation, whereas EGF promotes their proliferation ( xref )."
21917714
sparser
"As expected, EGF induced activation of both ERK1/2 and Akt as previously reported [ xref ]."
29520716
sparser
"In Mgat5 mutant and wild-type carcinoma cells, DAM-1976 enhanced and prolonged TGF-beta- and EGF-dependent Smad2/3 and Erk activation, respectively."
16585180
reach
"EGF Rapidly Stimulates the ERK Pathway in HT-29 Cells."
22988345
reach
"Epidermal growth factor-like ligands (e.g., EREG), which are expressed by VSMC, potently activate ERK and p38 MAPK, resulting in VSMC dedifferentiation [27]."
31861407
reach
"However, at 48 and 72 h, on RADA, RADA + RGD2 and RADA + RGD2 + HB peptide gels, tethered EGF induced statistically higher ERK activation than soluble EGF."
20304480
sparser
"However, it appears that S1P 1 knockdown has the capacity to abrogate EGF-induced ERK activation, implying S1P 1 is a highly influential regulator of ERK."
25197261
sparser
"Together, these data suggested that expression of PR through its PPD could reduce both the magnitude and the duration of EGF-induced activation of ERK1/2 in the absence of progestin."
26892043
reach
"The asymmetric unit Fig. 1 EGF triggered ERK and RSK activation in the presence of ORF45."
35078976
reach
"EGF stimulation of Erk was inhibited by AFC (N-acetyl-S-farnesyl-L-cysteine), an inhibitor of methylation, but not AGC (N-acetyl-S-geranyl-L-cysteine), an inactive analog of AFC."
14660603
sparser
"With respect to the EGF receptor, it has been reported that in parietal cells the PI 3-kinase inhibitor wortmannin had no effect on EGF-induced activation of ERKs [14] ."
11341977
reach
"EGF mediated inhibition of NGAL and E-cadherin expression involves the ERK pathway."
24048788
reach
"The established features of ERK1/2 activation by EGF, IGF-1 and insulin are that activated RTKs recruit Grb2, insulin receptor substrates (IRSs) and Grb2 associated binding protein 1 (Gab1) to interact with Shp2 leading to ERK1/2 activation [XREF_BIBR - XREF_BIBR]."
24521094
sparser
"EGF clearly activated Akt and Erk in adipocytes whereas it activated Erk in preadipocytes, but had only a slight effect on Akt and IκB ( xref )."
24719782
reach
"We show that the scaffold and adaptor protein CNK2 and MAGUIN -1 is required for NGF- but not EGF induced ERK activation."
15028221
sparser
"In PC12 cells, transient ERK activation induced by EGF resulted in proliferation, whereas sustained activation by NGF resulted in differentiation xref xref ."
26831618
reach
"This makes sense biologically because both EGF and HRG activate ERK through Ras and Raf."
19156131
sparser
"However, transient ERK1/2 activation induced by epidermal growth factor could not mimic the gene profile of the senescent hHSCs."
30426301
reach
"This promotion of EGF induced Erk1/2 activation is independent of the proteolytic activity of caspase-8 and can be recapitulated using only the pro domains of the protein."
19470771
reach
"In contrast, overexpression of wild-type Akt2 in MCF-10A did not affect ERK activation induced by EGF."
16365168
sparser
"Importantly, SHC1 knockdown largely abolished MIG6 depletion-induced EC tube formation and the increased ERK1/2 activation by EGF in ECs, suggesting that the anti-angiogenic function of MIG6 is mediated by its suppressive effect on SHC1."
33693003
sparser
"When used as a genetically encoded, intracellular reagent, NS1 potently inhibited EGF activation of ERK-MAPK ( xref )."
27820802
sparser
"Indeed, both EGF and serum induced ERK phosphorylation and activation in dose- and time-dependent manners, and importantly, the pSHOC2-Thr 507 levels correlated well with phospho-extracellular regulated MAPK (pERK1 and pERK2) levels ( xref and xref )."
30865892
sparser
"It appears Ras dimerization through this interface plays a negative role for EGF-induced ERK activation."
35522713
reach
"A well-known example of a FRET based activity reporter is the extracellular signal regulated kinase activity reporter (EKAR) [XREF_BIBR], which has been used to study oscillatory EGF mediated ERK activation and relationships between ERK activity and nucleocytoplasmic shuttling [XREF_BIBR, XREF_BIBR]."
27393828
sparser
"Inhibitors that blocked EGF-activated JNK, ERK, PI3K/Akt, and NF-κB were used to clarify the signaling pathways involved in the regulation of EGF-induced IL-1β expression."
23383347
sparser
"A classical example of this can be found in the MAP kinase pathway where transient ERK activation by EGF is associated with cell proliferation while persistent ERK activation by NGF leads to cell differentiation ( xref )."
21613981
sparser
"EGF-induced ATMCs display activation of the Erk1/2, PI3/Akt and Smad-dependent (Smad2/3) pathways."
24967966
reach
"On the other hand, EGF stimulates a transient ERK1/2 response in PC12 cells, leading to proliferation."
23380421
reach
"Previous studies using an ERK biosensor to investigate long-term ERK signaling in starved epithelial monolayers demonstrated that EGF treatment stimulated pulses of ERK activity over the course of days to regulate cell proliferation [51]."
36347198
sparser
"Since the initial observation that activation of ERK and PI3K by EGF is dependent on clathrin-dependent endocytosis [ xref ], endocytosis-dependent activation of ERK has been demonstrated in multiple receptor systems such as the insulin receptor [ xref ], the 5-HT receptor [ xref ], the m1 muscarinic receptor [ xref ] and the μ and δ opioid receptors [ xref , xref ]."
18675457
reach
"These results demonstrate that HRAS is competent for prolonging EGF induced ERK activation, and subsequently MCF-7 differentiation, only if its signals emanate from plasma membrane DM microdomains."
27099370
reach
"Robust phosphorylation (activation) of ERK was found in EGF treated cells, as predicted (XREF_FIG), whereas the EGF conjugated dendrimer induced minimal ERK activation."
20729136
reach
"Further, EGF receptors activate the extracellular signal regulated kinase (ERK) signaling pathway, which results in increased NF-kappaB and Sp1 transcription factors, followed by MUC5AC upregulation [XREF_BIBR]."
31737795
sparser
"We therefore investigated the effect of CIIA on the EGF-induced activation of Ras and Erk1/2."
22042618
sparser
"PKCzeta activity is required for EGF-induced extracellular signal-regulated kinase (ERK) activation in both normal human adult epidermal keratinocytes and five of seven SCCHN cell lines."
16778206
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"When ectopically overexpressed in murine fibroblasts and human glioblastoma cells, a constitutively activated human EGF receptor oncoprotein (deltaEGFR) induced EGF independent elevation of basal ERK MAPK activity."
10541432
reach
"EGF treatment significantly induced ERK activity (peaked at 30 min) and significantly increased COX-2 protein (peaked at 6 hr), production of prostaglandin E2 (PGE2), and cell proliferation."
14714610
sparser
"Consistently, AT2R inhibition of epidermal growth factor-activated extracellular signal-regulated kinase 1/2 was significantly reduced by the Rab1 mutants, indicating that endogenous Rab1 modulates the cell surface targeting and signaling of AT2R. It is of interest to note that Rab1 augmented the overall expression of AT2R and its mRNA, whereas the Rab1 mutants attenuated the total AT2R expression and enhanced ubiquitin-dependent AT2R degradation."
19357319
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"Additionally, we further validated whether the EGF signaling-mediated activation of ERK affected the conformational change of HSP70."
31558706
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"Moreover, we established that ERK1/2 activation by EGF leads to the mobilisation of alpha6beta4 integrin from HD."
22718344
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"In suspended cells, Fer expression resulted in an obvious phosphorylation level of Erk1/2 (pErk1/2) only when cells were treated with EGF, and the pErk1/2 level in adherent cells were much more enhanc[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
19339212
sparser
"AG1478 suppressed EGF-induced ERK1/2 activation at 2–5 min after the addition of EGF into the culture medium but it failed to suppress the ERK1/2 activation later ( Fig. 2 A)."
16414009
reach
"These results were consistent with the findings in XREF_FIG and XREF_FIG that suggested that beta-arrestin2 reduced EGF induced ERK activation by blocking the growth factor pathway."
19457093
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"Based on this information, we propose a model to explain the role of the ERK1/2 CC domain in EGF mediated ERK1/2 translocation and activation in focal adhesions."
19947948
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"Taken together, these experiments demonstrate that uPAR is a downstream target of GEP100 and Arf6 whose expression is required for EGF induced MDA-MB-231 cell invasion.Previous reports have shown that[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23747719
sparser
"However, our results revealed that EGF-induced ERK1/2 activation was resistant to PI3K inhibitors in BEL-7402 cells, regardless of the EGF concentration, suggesting that the role of PI3K in EGF-stimul[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16406609
sparser
"Furthermore, activation of ERK1/2 through EpEX-Fc and EGF in FaDu, Cal27, and Kyse30 cells was entirely blocked by an inhibitor of the upstream kinase MEK1 (AZD6244), whereas tyrosine kinase inhibitor (TKI) AG1478 entirely (Cal27) or partially (FaDu, Kyse30) blocked ERK1/2 activation by EpEX-Fc and EGF ( xref )."
30261040
sparser
"Because the PPD of PR can mediate the inhibition of EGF-induced A549 cell proliferation, we were prompted to investigate whether the presence of PR or the PR-PPD affected EGF-induced ERK1/2 activation, in the absence of hormone."
26892043
trips
"MDA-MB-231 cells, which express ERbeta, but not ERalpha, and low levels of GPR30 protein, are unable to stimulate adenylyl cyclase or promote estrogen-mediated blockade of EGF-induced activation of Erk-1/-2."
11773440
sparser
"For example, treatment of human breast cancer T47D cells with the MEK inhibitor U0126 in combination with the phosphoinositide 3-kinase/Akt inhibitor wortmannin synergistically suppressed EGF-induced ERK activation relative to treatment with U0126 alone [ xref ]."
24957684
reach
"Finally, EGF, which activates PLD independently of PKC, stimulates ERK in both cell types in a n -butanol-insensitive but PKC dependent manner."
12782152
sparser
"In fact, ERK activation by EGF appeared even to be stimulated upon PKC inhibition ( xref I)."
25421240
reach
"To demonstrate this consistency, we performed multiplexed single-cell measurements and applied computational modeling to determine how EGF signaling in the ERK and AKT pathways changes with cell phenotype."
33329028
reach
"Because EGFR signals through PI3-kinase and Akt and MAP and ERK effectors pathways, phosphorylation of Akt and ERK 1/2 were analyzed in ERbeta silenced REN and in MSTO-211H cells treated with EGF in the absence or in the presence of Gefitinib."
21124760
sparser
"Niacin showed a selected additive effect on chemoattractant-induced activation of ERK1/2, JNK and PI3K pathways, but only the MEK inhibitor UO126 reduced niacin-mediated inhibition of macrophage chemotaxis, while activation of ERK1/2 by EGF alone did not inhibit fMLF-mediated migration of HEK293T cells co-expressing HCA2 and fMLF receptor FPR1."
28186140
reach
"RNAi depletion of Mst2 or Lats1/2 in several cell lines was found to impair EGF induced Erk activation concomitant with diminished levels of the protein phosphatase 2A catalytic subunit and increased levels of the inhibitory RAF1 (Ser259) phosphorylation [XREF_BIBR]."
22898666
sparser
"In a study by Edwin et al. [ xref ], where Spry2 unexpectedly inhibited EGF activation of AKT and exhibited no significant effect on EGF activation of EGFR and ERK, they observed that Spry2 increases the amount and activity of PTEN that was found necessary for Sprouty to attenuate EGF-activated AKT and to inhibit cell proliferation."
24744103
reach
"In EGFR-Ishikawa cells, EGF leads to activation of the AKT and ERK1/2 pathways, which results in upregulation of the Snail protein, but not of Snail mRNA [XREF_BIBR]."
24459579
reach
"For instance, epidermal growth factor induces a transient extracellular signal-regulated kinase (ERK) activation in MCF-7 breast cancer cells, eliciting proliferation, whereas heregulin induces sustained ERK activation causing differentiation [139]."
| PMC
sparser
"An increase of intracellular Ca(2+) in PKHD1-silenced cells repressed the EGF-dependent ERK1/2 activation and the hyperproliferative response to EGF stimulation."
17669261
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"EGF exposure resulted in a transient, significant induction of PKC (81kDa) and ERK (43kDa) activation, with maximal ~ 2-fold (p < = 0.001) and ~ 1.6-fold (p < = 0.001) increases seen at 15 and 30min, respectively (XREF_FIG); activation of the other PKC and ERK proteins remained unaffected (XREF_FIG)."
27762399
sparser
"These distinct outcomes of Raf-MEK-ERK signaling are thought to result from transient and more persistent activation of ERK by epidermal growth factor and nerve growth factor, respectively xref , xref ."
26884720
reach
"The Erk inhibitor PD98059 blocked the EGF activated Erk as anticipated, whereas neither SB203580 nor SP600125 significantly inhibited EGF activated Erk (when normalized to expression levels of Erk as a loading control)."
25258648
reach
"However, although epidermal growth factor (EGF) treatment resulted in rapid activation of ERK1/2, it was not sufficient to promote E2F4 translocation into the nucleus or G1/S phase transition in HIEC."
23919615
sparser
"EGF (25 ng/mL) activated ERK in both types of cells and this effect was blocked by the MEK inhibitor, PD98059."
12753285
reach
"Furthermore, EGF activated EGFR, Erk and Akt in both SKpcDNA and SKCOX-1 cells, but aspirin inhibited EGFR, Erk and Akt activation in SKCOX-1 cells but not in SKpcDNA cells."
24155779
reach
"Furuchi and Anderson [XREF_BIBR] also detected increased EGF stimulated Erk activation following cholesterol depletion."
18949068
reach
"For example, EGF or heregulin (HRG) induce transient vs sustained activation of ERK and proliferation vs differentiation of MCF7 breast cancer cells."
19596615
reach
"In addition, the exogenous administration of EGF confirmed the requirement of ILK and IQGAP1 in EGF induced ERK1/2 activation, NF-kappaB activation, cell growth, and migration [XREF_BIBR, XREF_BIBR]."
25398317
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"Across all cell lines, EGF stimulation initiated a rapid ERK activity peak ~ 15 min after stimulation, followed by attenuation over 1.5–2 h to reach a steady‐state level (Fig 3B and C), with HRAS and NRAS cells exhibiting slower attenuation than any of the KRAS isoforms."
33073539
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"In the cytosol, EGF induces transient and HRG induces sustained ERK activation."
20493519
reach
"Though many of the same pathways are activated by the inflammatory stimuli IL-1α and TNFα, and the mitogenic stimulus EGF, these stimuli differ strongly in their magnitude of activation: IL-1α and TNFα more strongly activate the NFκB, JNK, and p38 pathways, while EGF more strongly activates the MEK/ERK pathway (Fig. 6A)."
29511118
reach
"Rapid and sustained nuclear cytoplasmic ERK oscillations induced by epidermal growth factor."
19953086
reach
"Whilst a complete mechanistic picture is lacking, studies in mammalian cell lines have revealed that Scrib depletion in EGF stimulated epithelial cells elevates ERK as well as JNK signalling [XREF_BIBR], and cell polarity perturbation leads to Hippo pathway impairment [XREF_BIBR, XREF_BIBR]."
29693007
reach
"EGF treatment of FaDu cells induced a rapid and strong activation of ERK1/2, whereas Cal27 cells were only moderately and more transiently activated (XREF_FIG)."
30261040
sparser
"Furthermore, using a prostate cancer cell line, we demonstrate that EGF induced activation of ERK and cell proliferation is attenuated by a chemical inhibitor that prevents the phosphorylation and act[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
10492251
reach
"Our results presented here (XREF_SUPPLEMENTARY) are consistent with those ffrom other groups, which have reported that compromising ubiquitin ligase activity of c-Cbl leads to enhanced EGF stimulated ERK1/2 MAPK activation."
19173594
sparser
"In our previous work, we already showed through modelling and experimental validation that the SOS feedback loop was not required for efficient signal termination and the transient activation of ERK induced by EGF [ xref ]."
19804630
sparser
"In PTX-treated cells, μ agonist inhibited EGF activation of ERK by ∼40%, in the presence or absence of Gα z transfection (Fig. 3A) ."
10989284
reach
"By contrast, in HKC cells the activation of ERK by EGF was not accentuated by 8-Br-cAMP; rather, the nucleotide decreased the expression of P-ERK to a small extent Figure 9 e and f."
12753285
reach
"Epidermal growth factor (EGF) also stimulated the activity of ERK, in both WKY and SHR cells, but nor of JNK or p38, with stimulation of ERK activity by EGF occurring more rapidly in SHR cells than in those from WKY rats."
10608465
reach
"Interestingly, inhibition of SphK1 with PF-543 or inhibition of SphK2 with K-145 drastically reduced EGF mediated P-ERK, P-AKT, and P-p38 in metastatic breast cancer LM2-4 cells (XREF_FIG)."
28108260
reach
"Specifically, phosphorylation of Y453 on GAREM was required for association with Grb2 and subsequent activation of Erk by EGF stimulation."
21799663
reach
"In Drosophila, increased EGF signaling activates ERK to promote sleep."
21784243
sparser
"By contrast, wild-type p53 failed to promote EGF-induced sustained ERK1/2 activation ( xref )."
23559009
reach
"In contrast, EGF induced ERK activation is independent of CNK2 expression."
15028221
sparser
"Furthermore, transfection of GEP100-△PH, inhibition GEP100 or Arf6 activity by GEP100 siRNA or Arf6 T27N failed to facilitate EGF-induced ERK and Rac1 activation."
22701712
sparser
"Calmodulin influences EGF-induced activation of ERK."
33766558
reach
"Thus, it is possible that NMDA receptor mediated Ca 2+ influx induces transactivation of EGF, which in turn activates ERK1/2."
19056470
reach
"In SKOV3 ovarian cancer cells, which bear a PIK3CA mutation and ERBB2 overexpression, ectopic VEPH1 expression enhanced EGF activation of ERK1/2, and mTORC2 activation of AKT."
36828346
reach
"As showed in Figure XREF_FIG, EGF activated ERK in a concentration dependent manner, and it exerted the best effect at a concentration of 100 ng for 1 h."
28428752
reach
"This is consistent with a recent report in which EGF triggered ERK dynamics regulating cell cycle entry required receptor dependent signaling events in addition to the feedback loops operating within the core Ras-Raf-MEK-ERK cascade (Sparta etal, 2015)."
26613961
reach
"EGF stimulation increased ERK *, but did not induce a high final ERK * (> 0.9) without HRG stimulation."
28542548
reach
"In a study by Impagnatiello et al. [XREF_BIBR] indicating an anti-proliferative effects of the overexpressed Spry1 and Spry2 on endothelial cells in the presence of FGF, VEGF, and EGF, while FGF- and VEGF induced activation of ERK were repressed by Sprouty, EGF activated ERK was left unaffected."
24744103
reach
"EGF mediated ERK activation is independent of G i proteins and PI3K and CDC42 and PAK pathways."
26262587
sparser
"Mechanistically, mutant p53-mediated miR-27a suppression increases cellular levels of EGFR and promotes EGF-induced ERK1/2 sustained activation."
23559009
reach
"Furthermore, activation of ERK1/2 by PDGF, but not EGF, required PKC activity."
15936927
reach
"In contrast, when EGF is used to elicit transient ERK activation, CHX has little or no effect (supplemental Fig. 2)."
18650424
sparser
"AG1478 effectively blocked the activation of ERK1/2 by EGF in Par-C10 ( xref ), HSG ( xref ), and HSY ( xref ) cells."
21397667
reach
"In gastrointestinal epithelia EGF induces MEK and ERK activation that, in turn, induces Rac1 activation."
23208503
sparser
"Because epidermal growth factor can activate both ERK1/2 and ERK5 [ xref , xref ], the remaining U0126-resistant K v 4.2 phosphorylation component may result from ERK5 activity."
29996472
reach
"In the studies presented herein that used freshly isolated pGCs, EGF induced activation of ERK occurred within 1 min of treatment, continued through 60 min, and began to decline thereafter."
10503724
reach
"For example, EGF activation of ERK combines digital and analog signaling mechanisms."
20855880
reach
"In U87 glioblastoma cells, ouabain did not inhibit EGF induced Erk1/2 or Akt activation and, surprisingly, further increased EGF induced EGFR phosphorylation."
25052069
trips
"Analyses with selective IQGAP1 mutant constructs indicated that MEK binding is crucial for IQGAP1 to modulate EGF activation of ERK."
16135787
reach
"To test this hypothesis, we examined the effects of SHP2 and PI3K inhibition, individually and in combination, on EGF mediated ERK1/2 and AKT activation, and cell proliferation."
24041688
reach
"8-Br-cAMP and EGF conjointly increased ERK activation above that of either agonist alone in ADPKD cells, and this combined effect was abolished by PD98059, indicating that ERK was activated by EGF- and cAMP responsive cascades that converge at MAPK."
12753285
reach
"In COS cells, it has been reported that EGF induced activation of ERKs is wortmannin sensitive, but only when very low levels of EGF receptors are activated [15]."
11341977
reach
"The opposite pattern was observed for ERK, with EGF eliciting the strongest ERK activity and IGF1 the least."
29886111
reach
"The results suggest that Fumos inhibits EGF stimulated ERK1/2 activation in a dose dependent manner in HeLa and HEK293T cell lines that contain wild type Ras."
26626996
reach
"The combination of EGF and lactoferrin increased DUSP degradation and triggered sustained ERK activation."
21610072
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"Epidermal growth factor (EGFy activates both mitten activated protein kinase (MILK) subtypes, the ERKs much more strongly than the JNK and SAFKs."
7709430
reach
"To systematically analyze the effect of ERK signal duration on mRNA expression dynamics and its relationship with cell fate determination, we first aimed to modify the EGF triggered ERK signal duration by changing EGFR activation dynamics via impairment of ubiquitination and hence the receptor degradation process."
25491268
reach
"The p-EGFR and p-Erk1/2 were examined to see whether EGFR signaling was activated upon EGF addition and we found that addition of EGF activates EGFR and Erk1/2 signaling (XREF_FIG)."
23333872
sparser
"On the other hand, LL-Z1640-2, a natural compound isolated as an anti-protozoan (Ellestad et al., 1978) , has been reported to inhibit anisomycin-induced JNK and p38 activation but not epidermal grow[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
11104828
sparser
"It was shown that activation of ERK1/2 and Snail by EGF in SiHa cells was prevented by SkQ1 suggesting that EGFR signaling critically depends on mtROS."
27902484
sparser
"Together, these data indicate that ERK activation by both LPA and EGF is required for synergism; however, signaling pathways in addition to ERK are also required since ERK activation does not exhibit [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12069834
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"Widely expressed with highest levels in bone marrow, spleen, brain, testes, and embryonic brain, Abi1 may negatively regulate cell growth and transformation by interacting with the nonreceptor tyrosine kinases ABL1 and/or ABL2, thus regulating EGF induced Erk pathway activation and EGFR signaling."
27557078
reach
"This behaviour was predominantly observed among DEGs and more pronounced upon EGF mediated ERK signalling than upon two-hour pulse signalling."
28468958
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"Insulin stimulated ERK1/2 activation was very sensitive to wortmannin and LY294002, but EGF stimulated ERK1/2 activation was resistant to these inhibitors."
16406609
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"These data, consistent with the Erk phophorylation results, confirm that hSef has opposite effects on FGF and EGF mediated Erk signaling.Under ordinary culture conditions, nerve growth factor (NGF) or[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18096367
reach
"Knockdown of SUMO1 did not affect Shp2 expression or protein stability according to the assessment as comparable by Western blotting, however notably inhibited EGF induced ERK activation in SMMC-7721 and HepG2 cells."
25823821
sparser
"Finally, our data showed that the native form of PEBP4 did not participate in ERK activation by EGF, although it seemed to be involved in the regulation of Akt activity."
27033522
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"Vertebrate EGF activates S. mansoni EGFR and the downstream classical ERK pathway, indicating the conservation of EGFR function in S. mansoni [XREF_BIBR]."
21548963
reach
"We found that in HaCaT cells ADAM17 depletion reduces ERK1/2 phosphorylation, independent from the treatment with PsVs.To solidify the hypothesis that ADAM17 acts via the ERK signaling pathway in HPV16 infection and entry platform formation, we restored ERK activation by the addition of recombinant soluble form of the epidermal growth factor (EGF) which is one of the EGFR-activating ligands."
31107240
reach
"Since 2-APB did not block EGF activation of the ERK pathway, the elevated level of intracellular calcium is a previously undescribed independent regulator of EGF induced gene expression that works together with ERK activation to regulate immediate early gene expression."
26551735
sparser
"We next examined a time course of EGF-dependent ERK1/2 activation in cells overexpressing either wild-type SOS1 or the SOS1 Ser1134Ala, Ser1161Ala double mutant."
22827337
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"In a comparable study using human cells, Pasic et al. showed that transient Erk activation by AREG promoted emergence of both luminal and myoepithelial cells while sustained Erk activation by EGF favored myoepithelial differentiation [XREF_BIBR]."
24124521
reach
"SUMO-1 and Ubc9 over-expression stabilize EGF induced ERK 1/2 activation."
21998680
reach
"Metformin pretreatment also inhibited EGF stimulated ERK kinase activation, as evidenced by decreased phosphorylation of ERK at Thr201 and Tyr204 (XREF_FIG), consistent with the notion that metformin inhibits PDX-1 expression through a mechanism involving inhibition of EGF- stimulated ERK signaling (XREF_FIG)."
26695692
sparser
"However, rapid activation of Erk by EGF did result in significant smad2 linker phosphorylation ( xref )."
22880011
reach
"This observation agrees with the previous report showing that Ras isoforms are redundant for EGF activated ERK activation and that HRas is a minor isoform in HeLa cells compared with KRas and NRas."
20071468
reach
"We also demonstrate that p53-273H-mediated suppression of miR-27a expression increases EGFR levels and enhances EGF induced sustained extracellular signal regulated kinase 1/2 (ERK1/2) activation, thus facilitating cell proliferation and tumor growth."
23559009
reach
"Because the bulk cultured cancer cells were supplemented with many unidentified factors in the serum, it may be possible that Kazinol-E suppressed ERK activity better in CSLC, in which ERK should be s[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
26774343
sparser
"EGF-induced PI3K/Akt and ERK activation was inhibited in propolin C-repressed EMT in EGFR wild-type lung cancer cells."
29681982
reach
"Thus, the ERK signaling pathway initiated by stimulation of either Ang II or the EGF receptors is Ca 2+ independent in WB cells.Since the maximal ERK activation induced by EGF or Ang II could not be a[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
9933031
reach
"By contrast, EGF induces transient Erk activation (as expected) -- as do all other EGFR ligands, including the low-affinity ligand AREG -- and fails to induce cell differentiation."
28988771
sparser
"Epidermal growth factor (EGF) and NGF both activate ERK and p38 in PC12 cells, but only NGF trigger differentiation."
11746502
sparser
"PKC-epsilon appeared specific for mechano-sensitive ERK1/2 activation, as antisense PKC-epsilon oligonucleotides did not inhibit ERK1/2 activation by EGF or bradykinin but did inhibit ERK1/2 activation upon EC adhesion to fibronectin."
9395450
reach
"Concentrating on PC9ER cells and contrasting EGF and IFN-γ, we verified the kinetic differences and noted that IFN-γ rapidly activated STAT-3, whereas EGF activated ERK (Figure S1A)."
34038737
sparser
"In PTx-treated cells, the cAMP content was increased and forskolin did not further inhibit CCK- and EGF-induced activation of c-Raf-1 or ERK1/2."
10953055
reach
"However, apocynin failed to alter the kinetics of the EGF stimulated ERK1/2 activation."
21268349
sparser
"In the last series of experiments, we investigated the time course for EGF activation of ERK in the freshly isolated pGC."
10503724
reach
"Treatment with the MEK1 inhibitor PD098059 (50 microM) abolished EGF induced ERK activation."
12644307
sparser
"Sur-8 expression enhances Ras- or EGF-induced Raf and ERK activation but has no effect on ERK activation induced by active Raf or MEK."
10783161
reach
"We therefore examined the relationship between SMYD3 and EGF stimulated ERK1/2 activation."
24847881
reach
"14-3-3 is known to have a critical role in the Ras-Raf signaling pathway through which growth factors such as EGF activate ERK1/2 by a phosphorelay from Ras-GTP and Raf1."
19996220
reach
"Similar results were obtained by evaluating ERK1/2 phosphorylation : PP1 prevents both SDF-1alpha- and EGF induced ERK1/2 activation (data not shown)."
15921680
reach
"Forced expression of Sprouty2 (Spry2) inhibits fibroblast growth factor (FGF) and vascular endothelial growth factor (VEGF), but not epidermal growth factor-(EGF) induced ERK activation [3]."
17689925
reach
"Whereas inhibition of EGF signaling clearly reduced the levels of active (phosphorylated) ERK in all three organoid types (i.e. P18T, NF1 and RASA1 KOs) after 1 hour of afatinib treatment, a substantial reactivation of ERK was observed in alive NF1 knock out organoids after 72 hours of afatinib treatment."
30858928
sparser
"We previously found that EGF activates ERK1/2 to stimulate goblet cell proliferation. xref As PKC is known to activate ERK1/2, we investigated whether myr-PKC α activates ERK1/2."
19074803
reach
"On the other hand, EGF activated ERK at the same time, which could target MMP9 directly to promote the migration ( Figure 6 )."
32174831
sparser
"As shown in Figure xref , activation of MEK1/2 and ERK1/2 by EGF was severely impaired in IEC-6/KRAS and DLD-1 cells knocked-down for SHP-2."
27582544
reach
"MyD88 independent activation of ERK by epidermal growth factor increased p-ERK and c-Myc and restored the multiple intestinal neoplasia (Min) phenotype in Apc (min/+)/Myd88 (-/-) mice."
27237322
reach
"Nevertheless, ouabain not only inhibited EGF induced Erk1/2 and Akt signaling, but also reduced EGF induced cell motility, and prevented the morphological changes in the actin cytoskeleton that were induced by EGF."
25052069
reach
"All three sites were central for EGF induced ERK activity and Cdk2 expression."
23168795
reach
"Mxi2 potently up-regulates ERK mediated activation of the transcription factors Elk1 and HIF1alpha but has no effect on the activity of ERK cytoplasmic substrates RSK2 and cPLA (2), induced by epidermal growth factor or by MEK."
12697810
sparser
"EGF-induced activation of ERK1/2 and Aurora A kinase was responsible for CPEB1 phosphorylation."
23824559
reach
"Moreover, GAP activity toward Ras could inhibit EGF induced ERK activation [XREF_BIBR], so the association of DAB2IP with ASK1 and the GAP activity of DAB2IP are both required for AIP1 enhanced ASK1 activity [XREF_BIBR]."
26658103
reach
"Wounding of the IEC-6 monolayer induced transient ERK activation, which was further enhanced by EGF."
15496479
reach
"A recent study, in both cell lines (androgen dependent LNCaP cells and androgen independent PC-3 cells) [42] and in xenograft models of the prostate cancer [43], indicates that the protein paxillin is[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
26742628
sparser
"Thus, these results suggest that the C-terminus of caspase-8 (which contains the Y380 site) does not directly contribute to the Erk pathway activation by EGF, and that the DED domains of caspase-8 are both necessary and sufficient to mediate Erk activation."
19470771
reach
"EGF stimulates ERK 1/2 activity leading to cell proliferation in both rat and human goblet cells with a similar magnitude and similar time course."
22975404
reach
"EGF activation of ERK prior to irradiation was associated with a marked increase in PARP activation and decreased survival in both cell lines."
17295209
reach
"Cdc42 activation inhibits the ubiquitination and degradation of EGFR; these actions result in cellular transformation due to EGFR accumulation and sustained EGF stimulated ERK activation XREF_BIBR."
30867840
sparser
"Given reports of ITSN1's contribution to ERK1/2 activation [ xref ], we also tested whether loss of ITSN1 or PI3KC2β altered EGF activation of ERK1/2."
25622909
sparser
"In comparison, LY294002 treatment had no effect on ERK activation by EGF, but the chemotactic response was reduced at all the concentrations of EGF tested indicating that NGF and EGF differed in the utilization of ERK and PI3-K to signal chemotaxis in PC12 cells."
15881653
sparser
"More specifically, we hypothesize that a pool of Shoc2 that is targeted to Rab7-positive endosomes is important for EGF-dependent ERK1/2 activation because this endosomal targeting occurs in an EGF and Ras-dependent manner."
22606262
reach
"Neurotensin and EGF induce synergistic stimulation of DNA synthesis by increasing the duration of ERK signaling in ductal pancreatic cancer cells."
15389644
sparser
"Several mitogens, including epidermal growth factor, platelet-derived growth factor, and ROS can activate ERK1/2 [ xref ] The activation of ERK1/2 by oxidative stress is consistent with the idea that low, but adequate levels of ROS are mitogenic [ xref ]."
31226872
sparser
"Control experiments showed that PTx had no effect on ERK activation by either VEGF or EGF (data not shown)."
19208746
sparser
"Pre-treatment of alpha T3-1 cells with the specific MAPK kinase (MEK) inhibitor, U0126, blocked PACAP and EGF-induced activation of ERK."
11739023
reach
"To determine whether G z could act as the transducer of the chronic opioid agonist actions on EGF stimulated ERK in the presence or absence of PTX, COS-7 cells were cotransfected with mu or kappa OR +[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
10989284
reach
"Constitutively active (GTPase deficient) Galpha12 and Galpha13 both inhibit ERK pathway activation by epidermal growth factor."
8702875
sparser
"In the studies presented herein that used freshly isolated pGCs, EGF-induced activation of ERK occurred within 1 min of treatment, continued through 60 min, and began to decline thereafter."
10503724
reach
"Moreover, although EGF is less able than HGF to reactivate ERK in most melanoma cell lines, 2 EGF and EGFR mediated resistance might be of interest in melanoma and in colon cancers.For driver mutation[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23026823
reach
"In this report, we examined the involvement of PI3K in insulin- and EGF stimulated ERK1/2 activation."
16406609
reach
"Many molecules such as IL-6 related cytokines (involved in inflammation), growth factors (i.e., EGF) and even ECM proteins such as fibronectin [59] activate STAT3 and ERK/MAPK signalings."
| PMC
reach
"Intriguingly, we demonstrated that the EGF induced Erk activation sustained by Sef leads to the neurite outgrowth in rat pheochromocytoma (PC12) cells.Monoclonal anti-Myc (9E10), anti-phospho-Erk1/2, [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18096367
reach
"Trastuzumab- and pertuzumab mediated effects on EGF induced ERK1/2 signaling were also minor in HCC1954 cells (XREF_SUPPLEMENTARY)."
23552733
reach
"In many systems EGF causes a characteristically transient activation of ERK."
22245064
sparser
"In cells where p14 was reduced by p14 siRNA2, EGF-induced ERK1/2 activation was only moderately affected after 5 min of EGF stimulation."
12479806
reach
"Santos et al. used the SSPRs and pseudo-SSPRs to calculated the LRCs for NGF and EGF stimulated ERK pathway 15 respectively."
30076370
sparser
"Although overexpression of N-terminal tagged PEBP4 resulted in an inhibition of ERK activation by EGF, that with a C-terminal epitope tag did not have such an effect."
27033522
reach
"Down-regulation of GEP100 or Arf6 by specific siRNAs largely inhibited EGF induced ERK activity."
23747719
reach
"Whereas treatment with lapatinib diminished EGF stimulated activation of Erk1/2 and IRS-1 (Y896), it did not significantly attenuate IGF-I- or IGF-I+ EGF induced activation of Akt and IRS-1 (Y612)."
25886138
reach
"Preliminary results form our laboratory have also shown that Raf kinase activity in primary cultures of pGC is markedly increased by EGF treatment, suggesting that EGF stimulates ERKs in pGC through a[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
10503724
reach
"Shp2 is known to mediate EGF stimulated Erk1/2 activation [XREF_BIBR]."
20510203
reach
"EGF administration to mock transduced PC12 cells resulted in a very rapid but transient ERK1/2 activation that peaks within the first 5 min."
17224080
sparser
"AG-1478 also suppressed both EGF-activated Akt and Erk (figure xref D)."
25258648
sparser
"The SQ20B head and neck squamous carcinoma cell line demonstrated loss of EGF-dependent AKT and ERK activation even at low doses of inhibitor."
14612544
sparser
"We found that SB203580 by itself did not affect the activity of ERK1/2 but significantly extended EGF-induced ERK activation in PC12 cells, which resulted in early morphological differentiation."
11746502
reach
"beta (2) AR dependent signaling to ERK1/2, like direct EGF stimulation of ERK1/2 activity, is sensitive to inhibitors of clathrin mediated endocytosis, suggesting that signaling downstream of both the EGF activated and the GPCR transactivated EGFRs requires a productive engagement of the complex with the cellular endocytic machinery."
10734107
reach
"In sharp contrast, although serum, LPA, and EGF stimulated ERK1/2, in these short-term assays, SK1-I did not significantly affect stimulated ERK1/2 phosphorylation at Thr202 and Tyr204 (XREF_FIG)."
19723667
reach
"EGF activated predominantly ERK pathway."
21440529
reach
"It was shown that EGF stimulation causes a strong but transient activation of the ERK cascade, while NGF causes an equally strong but sustained activation; 18 and both effects were shown to be dependent on the activity of ERKs."
20201891
reach
"This suggestion is supported by results of Stefanovsky et al. (2001) showing that epidermal growth factor induces ERK dependent activation of ribosomal gene transcription."
12620228
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"However, SK1-I did not reduce EGF- and serum induced ERK1/2 activation in both U373 (XREF_SUPPLEMENTARY) and LN229 cells (XREF_FIG)."
19723667
reach
"In contrast, erlotinib, a small-molecule EGFR tyrosine kinase inhibitor, completely abolishes EGF-mediated ERK reporter activities (Figure 5c)."
35868851
sparser
"Caspase-8’s “RXDLL motif” is critical for EGF-induced Erk pathway activation."
19470771
reach
"Our recent findings demonstrate that Erbin exerts dual functions in ERK signaling pathway in cardiomyocytes, either as a negative regulator to suppress EGF induced ERK activation or as a positive regulator to enhance catecholamine stimulated ERK activation XREF_BIBR."
22880131
reach
"Despite the reduced amount of c-Raf and Erk2 in the cholesterol depleted caveolae fraction, EGF caused a hyperactivation of the remaining caveolae Erk isoenzymes."
9694863
reach
"In contrast, no change in EGF induced ERK cascade activation was detected at the 2 hr time point, whereas at the 18 hr time point, the levels of EGF induced pMEK, pERK, and Raf dimerization were drama[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
27889448
reach
"It was reported that EGF activates ERKs mainly through the Grb-2-SOS-Ras-Raf-MEK-ERK pathway [XREF_BIBR]."
23496982
sparser
"TU-100 and ginseng and to a lesser extent ginger or 6-gingerol, but not Japanese pepper, ginsenoside Rb1 or compound K inhibited EGF activation of ERK1/2."
27730672
sparser
"PP2 did not suppress EGF-induced ERK1/2 activation ( Fig. 2 A)."
16414009
sparser
"Importantly, when used at physiologically relevant concentrations, EGF, insulin and IGF-I activated the 81 kDa PKC and ERK, 116 kDa PKC and ERK, and 116 kDa PKC, respectively and at different times."
27762399
reach
"Spry2 inhibits FGF induced ERK activation by interfering with FRS2/Shp2/Grb2 interactions XREF_BIBR, whereas it activates EGF mediated ERK activation by preventing c-Cbl-mediated ubiquitination and endocytosis of activated EGFR XREF_BIBR."
18335055
sparser
"Treatment with the MEK1 inhibitor PD098059 (50 microM) abolished EGF-induced ERK activation."
12644307
reach
"Activation of ERKs by EGF receptors [49 **] and IGF-1 receptors [50 *] is blocked by agents that inhibit clathrin mediated receptor endocytosis."
10209148
sparser
"Fumos inhibits EGF-induced ERK1/2 activation."
26626996
sparser
"The EGF-induced activation of Erk1/2 was also apparent in MDCK/control cells but not in MDCK/CIIA-Flag cells ( xref )."
22042618
sparser
"To investigate the role of PKC in Ang II- and EGF-induced activation of ERK, WB cells were incubated with GF-109203X prior to the addition of EGF and Ang II."
9933031
reach
"As a control we studied EGF induced ERK1/2 activation in the primary chicken Muller cells."
27930693
trips
"Identification of a dominant negative mutant of Sprouty that potentiates fibroblast growth factor- but not epidermal growth factor-induced ERK activation."
11495895
reach
"EGF activated IkappaB, Akt and Erk whereas TNF activated only IkappaB and reduced EGF activated Akt and Erk in these cells."
23800251
reach
"EGF driven activation of ERK was also not inhibited by the PAK1 inhibitor IPA-3 or the CDC42 inhibitor ML141 (XREF_FIG, respectively)."
26262587
reach
"Indeed, EGF enhanced slightly Mn 2+ -induced cell adhesion and ERK activation (not shown)."
17275949
sparser
"NS5A containing mutations within the C-terminal proline-rich motif neither bound Grb2 nor inhibited ERK1/2 activation by EGF, demonstrating that NS5A-Grb2 binding and downstream effects were due to direct interactions."
12186904
sparser
"In agreement with previous studies ( Quintanar-Audelo et al., 2011 ), the expression of Myc-tagged wild type (WT) Spred2 in 293T cells remarkably reduced EGF-induced ERK1/2 activation while deletion o[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
22305891
sparser
"S1P has been shown to activate ERK in Swiss 3T3 fibroblasts, and the SphK inhibitor DHS blocked PDGF- but not EGF-induced activation of ERK, which was reversed by exogenous S1P [80] ."
12531554
trips
"Grb2 interacts with SGEF and antagonizes the ability of SGEF to enhance EGF-induced ERK1/2 activation."
24399467
reach
"We next investigated whether miR-27a regulates EGF induced ERK1/2 activation."
23559009
reach
"Since either the ERK or the BMK1 pathway was activated by EGF treatment, further studies were performed to confirm which was involved in the proliferation of the prostate cancer cells."
26870175
reach
"A rapid phosphorylation expression of ERK1/2 (molecular weight at 44 kD and 42 kD, also called as p44 and p42) was observed by treatment of the BEAS-2B cells with 100 microg/ml crocidolite or 100 ng/ml EGF (the proven activator of the ERK signaling pathway) at 30 minutes."
17096943
reach
"Since EGF activated Erk had no significant effect on cell viability, we investigated the Akt pathway, which is another key downstream signaling linked to EGFR activation."
25258648
reach
"Therefore, Fumos inhibits EGF induced activation of ERK1/2 downstream of Ras in cells expressing wild type Ras."
26626996
sparser
"Although it is well known that the small GTPase, Ras, plays a pivotal role in growth factor-induced activation of ERK1/2, it is also possible that insulin and EGF could activate ERK1/2 via a Ras-indep[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16406609
reach
"EGF dependent activation of Ca 2+ influx as well as PLC-gamma, Shc, and ERK MAPK was normal in both CAML negative and CAML positive cells, however."
12919676
reach
"Moreover, Rabex5 and Rabaptin5 are tightly bound, and the complex does not dissociate, even after Rab5 activation , whereas binding of SPIN90 and Gapex5 is signal-dependently regulated by the phosphorylation status of SPIN90, which is determined by EGF-induced ERK signaling."
31358736
reach
"Activation of Erk signaling by EGF or PMA also induces the G2 phase delay and the blocked exit from G2 checkpoint by destabilizing Cdc25B [170]."
| PMC
sparser
"It was revealed in a study by Sasaki et al. [ xref ] that the expression of Spry2 and Spry4 in HEK293 cells inhibited FGF-induced ERK signaling but did not affect EGF or PDBu activation of ERK."
24744103
reach
"Moreover, in agreement with previous work demonstrating that the M-Ras and Shoc2 interaction is required for efficient EGF induced ERK activation, only the Shoc2 proteins competent to bind M-Ras could restore EGF induced ERK activation in Shoc2 depleted cells (SI Appendix, Fig."
30808747
reach
"Interfering with this signalling platform has dramatic consequences on EGF mediated ERK1/2 and STAT3 activations, as well as on EGF dependent EMT."
25366117
reach
"Binding of EGF to its receptor triggers rapid activation of the ERK pathway, but its plethora of positive and negative feedback loops can result in complex patterns of signaling (Kochanczyk et al., 20[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
29284127
reach
"While several studies have suggested that Erk activation can be regulated through the PI-3-kinase pathway [XREF_BIBR, XREF_BIBR] our data demonstrated that EGF stimulation of Erk activation in HC11 mammary epithelial cells was not altered by blocking PI-3-kinase signaling with LY294002."
16984645
reach
"H (2) O (2) - and EGF induced ERK1/2 activation is mediated by EGFR, whereas Akt is activated by Src independent of EGFR following H (2) O (2) exposure."
15075181
sparser
"As shown in xref , the kinetics of EGF-dependent ERK activation in HT-29 cells are consistent with the possibility that the EGFR couples TNF to ERK activation."
22988345
reach
"Dihydrotestosterone potentiates EGF induced ERK activation by inducing SRC in fetal lung fibroblasts."
24484548
reach
"Third, experiments and model simulations show that wortmannin affects the magnitude of EGF induced ERK signaling at times greater than 5 min, but does not change the qualitative shape of the response."
18004277
sparser
"Although both EGF- and insulin-induced ERK1/2 activation can occur via a Ras-independent pathway in fibroblasts and 3T3-L1 adipocytes ( Carel et al., 1996 ; Burgering et al., 1993 ), ERK1/2 activatio[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16406609
reach
"For that we pretreated A549 and dasatinib, gefitinib and ulixertinib blocked EGF-driven ERK activation in A549 cells (Fig. 4c,d and Supplementary Fig. S4b )."
34045585
reach
"As shown in Fig. 4 A and B, overexpression of dominant negative mutant form of PAK2 enhanced synergistic activation of p38 or ERK in HM3 cells treated with NTHi and EGF, whereas overexpression of domi[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17555715
reach
"In PC12 cells however, EGF causes transient activation of Ras, MEK and ERK, whilst NGF induces sustained activation of these signalling molecules [1]."
11024454
reach
"Introduction of a SAICAR-insensitive or a non phosphorylatable PKM2 mutant inhibited the EGF stimulated ERK activation and consequent cancer cell proliferation, suggesting SAICAR induced PKM2 protein kinase activity is required for EGF mediated cancer cell proliferation [XREF_BIBR]."
33292198
sparser
"It is well-established that Ras/ERK and PI3K/Akt are two major signaling pathways downstream of EGFR. xref Indeed, EGF treatment of MDA-MB-468 cells activated ERK and Akt ( xref ), while the EGFR inhibitor AG1478 abolished the effect of EGF ( xref )."
25124473
sparser
"It was reported that EGF-induced ERK1/2 activation occurs mainly through EGFR activation in porcine renal cell ( xref )."
34093234
sparser
"In addition, EGF activated Akt and Erk whereas TNF solely activated IκB in OVCAR-3 cells (Figure  xref C)."
23800251
reach
"Mutation of these residues, while having no effect on basal gap junction formation and function, prevents cell uncoupling in response to the ERK activator EGF."
11449001
reach
"Both MEK inhibitors UO126 and PD184352 completely abolished the activation of ERK by EGF and snSPIONs."
23162692
reach
"Due to the lack of appropiate techniques it has not been analyzed in nociceptive neurons if there are differences in the kinetics of Erk1/2 in response to NGF versus EGF.Therefore‚ we investigated if nociceptive neurons express the EGF-receptor and if EGF results in activation of Erk1/2 similar to the growth factors NGF and GDNF."
21187008
reach
"A similar finding has been described with Herstatin, which uncouples the Akt and Erk pathways that are activated by EGF and the EGF receptor (EGFR,)."
27658374
reach
"Although EGF induced NF-kappaB activation was defected in CARMA3 or Bcl10 knockdown cells, EGF induced ERK activation was not affected in these cells (XREF_FIG)."
21406399
reach
"For example, the mitogen EGF induces a transient ERK activation, whereas the neurotrophin NGF induces prolonged ERK activation."
15028221
sparser
"This suggests that PAK5 cannot contribute to EGF-dependent Raf-1 or ERK activation in the cytoplasm."
18465753
reach
"Specifically, in neural stem cells, BTC activates both the Erk and Akt pathways, whereas EGF mainly activates the Erk pathway, which ultimately promotes neural stem cell proliferation 27."
28922560
sparser
"Experiments conducted on a prostate cancer cell line demonstrated that EGF induced activation of ERK and cellular proliferation was partially inhibited by PD98059, a chemical inhibitor of the immediate upstream signaling component responsible of activation of ERK."
10492251
sparser
"Therefore, it is reasonable to speculate that EGF-induced ERK activation is indispensible for GEP100/Arf6 mediated breast cancer cell invasion."
23747719
reach
"As our data suggest that GH synergistically augments EGF induced ERK activation in 3T3-F442A cells, we sought to localize the level (s) in EGF induced downstream ERK signaling at which GH exerts this effect."
20946955
reach
"Pre-incubation with BIM blocked EGF induced ERK 1/2 activation and Pit-1 expression."
21075822
reach
"PAK5 expression does not potentiate EGF stimulated ERK and MAPK activation."
18465753
sparser
"Similarly, Kim et al. found that phosphorylation of the Nicastrin subunit by EGF-activation of ERK1/2 reduces γ-secretase activity ( xref )."
21982365
reach
"To our knowledge, the present study is the first to suggest the potential prognostic significance of FOXO3a mediated miR-622 transcription that then downregulates HIF-1alpha and decreases tumor aggressiveness in response to EGF activated ERK signaling in lung cancer."
26528854
sparser
"EBP50 knockdown abolished its inhibition on EGF-induced ERK activation, suggesting EBP50 regulated EGFR signaling."
27509846
reach
"Conversely, EGF induced ERK activation spreads throughout the cell body after EGF bead stimulation."
12952932
reach
"In addition, Fujiwara et al. (1997) reported that 3,4-dephostatin, a protein-tyrosine phosphatase inhibitor, sustained for 1-3 h the tyrosine phosphrylation and activation of ERK1/2 induced by NGF and[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
11301055
reach
"Consistent with these results, we found that inhibiting p38 MAPK activation prolonged and/or increased EGF induced ERK and JNK activities in human skin fibroblasts."
21872446
sparser
"In contrast, EGF-induced ERK activation was inhibited by AG 1478, but not by cell exposure to galardin."
14568001
sparser
"Real-time imaging of cells expressing EKAR demonstrated that depletion of AKAP-Lbc inhibited the onset, and almost abolished, EGF-dependent ERK activation when compared with cells treated with control siRNA ( xref )."
21102438
sparser
"Band density measurements further confirmed that inhibition of EGFR suppresses EGF-induced ERK1/2 and Akt activation in HER2 overexpressing cells ( xref )."
21911055
sparser
"Moreover, treatment of cells with epidermal growth factor (EGF) that activates MEK and ERK, as well as genetic ERK activation ( xref ) by a constitutively active MEK (MEK CA ) ( xref ), increased T562 phosphorylation levels; pharmacological or genetic MEK inhibition using the selective U0126 inhibitor or by expressing a dominant negative MEK mutant (MEK DN ); and reduced T562 phosphorylation; finally, in Mfn1 −/− cells re-expressing the MFN1 T562A mutant, phosphorylation was undetectable even in the presence of EGF ( xref G)."
25801171
reach
"On the other hand, epidermal growth factor causes a prolonged activation of Raf-1 kinase and ERK activity and a smaller, more transient activation of JNK, whereas the phorbol ester phorbol 12-myristate 13-acetate causes a small stimulation of Raf-1 kinase and a pronounced stimulation of ERK activity."
7929360
sparser
"Matrix stiffness has previously been shown to increase growth-factor-dependent Erk activation through focal adhesion assembly [ xref ], providing an explanation for our observed differential response in PDGF-induced proliferation."
25085859
sparser
"These data show that hNHE1 is important for EGF-induced ERK1/2 activation in a cellular context, in a manner dependent on its D3-domain and F2-site."
27083547
reach
"In these cells, EGF leads to a transient activation of ERK with cytoplasmic retention resulting in a proliferative cellular response."
22404972
reach
"Addition of EGF activates ERK1/2 in a ' wild-type ' rabbit corneal epithelial cell line [XREF_BIBR], and exerts positive effects on epithelial wound healing by promoting migration and mitosis [XREF_BIBR, XREF_BIBR, XREF_BIBR - XREF_BIBR]."
16914058
reach
"Combined treatment with GH and EGF did not augment more than additively either Akt or PLC-gamma however, ERK was synergistically activated by combined GH and EGF treatment, consistent with our previous findings."
20946955
sparser
"To determine if G i αPCRs are involved in the activation of ERK1/2 by EGF, we treated cells with G i α protein inhibitor PTX."
24521094
reach
"We demonstrated an EGF induced ERK nuclear accumulation in SMMC-7721 and LO2 cells, which may probably be associated with Tensin4 expression."
26035355
reach
"All of these results showed that ER-alpha36 mediated the EGF stimulated ERK activation in Hec1A cells."
21888973
reach
"The combined data indicate that 1) EGF, TPA, and carbachol activate overlapping as well as distinct intracellular signaling pathways in gastric parietal cells, 2) EGF activates ERKs and enhances parietal cell acid secretory related functions via receptors with similar affinities, and 3) in contrast to some cell types, the parietal cell ERK signaling cascade does not appear to be directly modulated by the PtdIns 3-kinase pathway or by elevated intracellular free Ca2+ or adenosine 3 ',5 '-cyclic monophosphate concentrations."
8897883
reach
"On the other hand, H89 had no effect on the activation of ERK by EGF, although the PKA inhibitor diminished P-ERK expression when EGF and 8-Br-cAMP were added conjointly Figure 9 c and d."
12753285
reach
"As shown in XREF_FIG above, both atRA (100nM 1hr) and EGF rapidly activated ERK1/2 in ESC; however, a lipid raft inhibitor, methyl-beta-cyclodextrin (MBCD), effectively abolished EGF activated ERK1/2 but failed to inhibit atRA triggered rapid ERK1/2 activation."
22982089
reach
"Since the results presented here indicate that ERK mediates positive regulation of PDX-1 expression by EGF and metformin has recently been shown to inhibit ERK activation in PDAC cells [XREF_BIBR, XREF_BIBR, XREF_BIBR], we hypothesized that the metformin inhibits PDX-1 expression by abrogating EGF induced ERK activation."
26695692
sparser
"Use of suboptimal EGF concentrations revealed the potentiating action of PTHRP on EGF-induced ERK activation more clearly ( xref )."
12592371
reach
"In Swiss 3T3 fibroblasts EGF elicits transient ERK activity and negligible induction of the FOS IEG, whereas platelet derived growth factor (PDGF) elicits sustained ERK activity and a substantial FOS response XREF_BIBR."
20495582
reach
"EGF also stimulates RAS and ERK but in a transient manner."
8662831
sparser
"Calcium-induced ERK activation differed in kinetics from mitogenic ERK activation by epidermal growth factor and could be modulated by alterations of intracellular calcium levels."
11018025
reach
"A short pulse of EGF could stimulate Akt/Erk signaling via activation of EGF-EGFR cascade, however, decreasing TFG using siFUT1 or UEA-I treatment reduced the EGF-EGFR activation and Akt/Erk signaling."
31754244
reach
"These data suggest that EGF activates downstream Akt and ERK signaling via EGFR and PAFR."
24721622
reach
"In a two-hybrid screen, the catalytic domain of PMCA4b was found to interact with RASSF1 (via amino acids 144-193 of RASSF1A or amino acids 74-123 of RASSF1C), with co-expression in cells causing inhibition of the epidermal growth factor (EGF)-dependent activation of the Erk pathway (Erk is a downstream target of the Ras-Raf-MEK signalling cascade that activates cellular proliferation, see XREF_FIG) XREF_BIBR."
17692468
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"Furthermore, GPx3 could attenuate EGF (Epidermal Growth Factor) and HGF (Hepatocyte Growth Factor) induced Erk activation through the restoration of MKP3 expression."
25333265
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"Epidermal growth factor stimulates Rac1 activation through Src and PI3K to promote colonic epithelial cell migration XREF_BIBR and induces the ERK and Rac1 signaling pathway to promote cell migration in human hepatoma HepG2 cells XREF_BIBR."
26078726
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"To this end, we assessed the ability of wild-type and mutant SHP2 enzymes to dephosphorylate Paxillin and pY118, a physiological substrate of SHP2 required for EGF stimulated ERK1/2 activation."
24935154
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"However, in breast cancer cells, abolishing PRMT5 mediated EGFR Arg1175 methylation increased EGF induced extracellular signal regulated kinase (ERK) activation by preventing the recruitment of Src homology 2 domain containing protein tyrosine phosphatase (SHP) 1 to EGFR, leading to enhanced cell proliferation, migration, and invasion of EGFR overexpressing tumor cells [XREF_BIBR]."
33420374
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"While EGF, TPA, and activated Ras cause maximal ERK activation (Cobb et al., 1991; Sturgill and Wu, 1991; Davis, 1993), some of these agents caused only a small increase in JNKi activity."
8137421
sparser
"Conversely, EGF-induced ERK activation spreads throughout the cell body after EGF bead stimulation."
12952932
reach
"Selective inhibition of the EGF receptor (EGFR) with AG1478 blocked ERK and p38 activation at both concentrations; however, selective inhibition of the ErbB2 receptor (EB2R) with AG825 or small interfering RNA (siRNA) blocked low but not high EGF activation of ERK and p38."
17372305
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"We show that while ouabain did not directly affect EGFR and ErbB2 phosphorylation, it inhibited EGF induced activation of Erk1/2 and Akt signaling."
25052069
sparser
"Expression of the insert in cells reduced activation of ERKs by EGF."
9677429
sparser
"Inhibition of p38 with SB203580 was found to prolong the EGF-induced ERK activation in PC12 cells, causing differentiation of the cells [48] ."
14499638
sparser
"Because Ras participates in Erk activation by epidermal growth factor (EGF), we tested whether Ras methylation regulates Erk activation."
14660603
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"We showed that NS5A inhibited epidermal growth factor mediated ERK and Egr1 activations and this inhibitory activity of NS5A was nullified in Abi1-knockdown cells."
27551040
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"Expression of a constitutively active Rap1 (RapV12) inhibited the ERK activation induced by LPA and EGF in Rat-1 fibroblasts [7]."
10675535
sparser
"Experiments involving the expression of the dominant-negative mutants of Ras and Rap1 signaling (RasN17 or Rap1N17) indicate that both GTPases Ras and Rap1 are recruited for the ERK activation by VIP and PACAP38, whereas Rap1 is poorly involved in TRH or EGF-induced ERK activation."
14551200
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"As morphine had not been previously tested for its long-term effect on EGF activation of ERK, it was examined and found to mimic the DAMGO action (XREF_FIG)."
19457093
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"In addition, in D283 cells, a cell line derived from medulloblastoma metastasis in the peritoneum, ouabain did not inhibit EGF induced activation of Erk1/2 but reduced EGF induced Akt activation and almost completely abolished EGF induced EGFR phosphorylation."
25052069
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"AACOCF3 did not influence EGF induced ERK1/2 activation, indicating that ERK1/2 is upstream of PLA (2)."
12138128
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"The role played by Cdc42 in regulating the timing of EGF receptor-Cbl interactions is underscored by the fact that constitutively active Cdc42 (F28L), by persistently blocking the binding of Cbl to these receptors, leads to their aberrant accumulation and sustained EGF stimulated ERK activation, thus resulting in cellular transformation."
14505571
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"EGF induced ERK activation downstream of FAK requires rac1-NADPH oxidase."
21660950
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"As we previously demonstrated [23], both EGF and FGF activated ERK with similar time courses in MCF-7 cells."
22227580
sparser
"Indeed, ouabain inhibited EGF-induced Erk1/2 and Akt activation and, moreover, prevented EGF-induced formation of actin stress fibers and cell motility, probably by activating a stress signaling response."
25052069
reach
"Our results demonstrate that EGF stimulates conjunctival goblet cell secretion by binding to its receptor to increase [Ca 2+] i and activate ERK 1/2."
22975404
sparser
"Fibroblast-Type II cell communications are dependent on extracellular signal-regulated kinases (ERK) 1/2 activation by the ErbB receptor ligands epidermal growth factor (EGF), transforming growth factor (TGF)-α, and neuregulin (Nrg)."
24484548
sparser
"Neutralizing antibody against alpha5beta1 integrin partially (approximately 60%) blocked BK-induced ERK activation but did not affect EGF-induced ERK activation."
20385709
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"The Na+ effect was concentration dependent (maximal effect, </= 12.5 mM) and was specific for shear stress mediated ERK1/2 activation as epidermal growth factor stimulated ERK1/2 activation was unaffected by removal of extracellular Na+."
10400628
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"Surprisingly, epidermal growth factor (EGF) caused a strong dose dependent augmentation of radioiodide transport, accompanied by extracellular signal regulated kinase (ERK)-1/2 activation."
18997042
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"While GHR knockout is associated with diminished expression of the EGFR and a concomitant decrease in EGF signaling, GH overexpression results in EGFR overexpression with different effects depending on the signaling pathway analyzed : AKT and ERK1/2 pathways are induced by EGF, while STAT3 and STAT5 activation is heterologously desensitized."
20032199
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"Although Spry1 and Spry4 heterodimers potently inhibited FGF2 mediated ERK activation, it had little effect on EGF mediated ERK activation."
23554919
reach
"XREF_BIBR, XREF_BIBR, XREF_BIBR EGF promotes EMT through the upregulation of Snail and Slug and activates PI3K and Akt and ERK signaling pathways in lung cancer."
28009353
sparser
"Hence, in addition to an inhibitory role of EpEX on the strong activation of ERK1/2, Snail, Zeb1, and Slug by EGF, EpEX has itself an intermediately activating effect on ERK activation in serum-starved HNSCC lines that results in proliferation."
30261040
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"Furthermore, EGF induced Erk activity appears to be dependent on PKC (Corbit et al., 2000)."
12615082
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"EGFR endocytosis from the plasma membrane to early endosomes is required for the full spectrum of EGF mediated ERK1/2 and PI3K and AKT signalling XREF_BIBR."
27387133
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"ERK activation by EGF treatment of serum starved cells did not significantly affect the interaction, nor did mutation of the D-domain binding site in ERK2 (D319N) (XREF_FIG) XREF_BIBR."
21887333
sparser
"In concordance, knockdown of EpCAM in Kyse30 cells resulted in enhanced ERK1/2 activation by EGF in our in vitro experiments."
30261040
sparser
"Results Phosphorylated ERK1/2, which was activated by EGF, promoted LF proliferation by accelerating cell-cycle progression from the G1 to S phase."
33083482
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"Taken together, these data indicate that nucleobindin-2 regulates EGF stimulated MAPK kinase and Erk signaling, cell proliferation, and adipocyte differentiation."
22514047
sparser
"EGF-induced ERK1/2 and Akt activation in the presence of HER2 followed a revamping pattern ( xref ), a clue provided by time course EGF stimulation experiments."
21911055
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"Interestingly, the unexpected results repeatedly show that EGF induced ERKs ' and RSKs ' phosphorylation was increased by fargesin treatment in a dose dependent manner (XREF_FIG b)."
33669811
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"We found that HER2 mediated effects on EGFR dimerization and trafficking were sufficient to explain the observed HER2 mediated amplification of epidermal growth factor induced ERK signaling."
15572377
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"SP, PGE 2, and EGF activated Erk1/2 and stimulated proliferation of Caco-2 cells."
20944107
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"We found that the activation of Erk by EGF stimulation was attenuated in the cells under overexpression of GFP-Rab21, suggesting that Rab21 reduces the duration of EGFR activation indicated by Erk pho[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
22525675
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"We demonstrate here that the pan-ErbB kinase inhibitor CI-1033 was effective at inhibiting EGF mediated phosphorylation of Tyr1221 of ErbB2, Tyr1068 of EGFR, and EGF mediated activation of ERK."
18927496
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"Thus, inhibition of PKHD1 can cause EGF induced excessive proliferation through decreasing intracellular [Ca2+] i, resulting in EGF induced ERK1/2 activation."
24336431
sparser
"Consistent with phosphorylation of these sites contributing to negative regulation of Ras-MAPK signaling, we observed an increase in the magnitude and duration of EGF-dependent ERK1/2 activation ( xref )."
22827337
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"The treatment of the cells with the ERK activator epidermal growth factor (EGF, 200 ng/mL) decreased the protection effect of ATF6 on IR."
21841811
sparser
"As expected, EGF activated ERK1/2 in all epithelial cells, but not in THP1 cells."
29467931
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"Here we show that the EGF mediated ERK activation is abolished by loss of Grb2, whereas this response is not affected by loss of Shc."
10400627
trips
"Also PKHD1-silencing lowered the intracellular Ca(2+) and caused EGF-induced ERK1/2 overactivation in the cells."
17669261
sparser
"Additionally, both FGF-1 and EGF activated extracellular signal-regulated kinase (ERK) in FLS1 cells."
32782506
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"An alternative pathway of PIP 3 -dependent ERK activation involves phosphoinositide dependent kinase-1 (PDK1), a target of PI3K, which can activate MEK directly and ERK indirectly through the activation of certain PKC isoforms, although it has been reported that PKCs are not involved in EGF induced ERK activation."
19357636
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"The kinetic profile of extracellular signal regulated kinase (ERK) signals demonstrated that ERK1/2 activation was rapidly and strongly induced by EGF but delayed and less marked by TGF-beta1 stimulation."
17467690
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"The results demonstrated that CHAG treatment efficiently blocked the phosphorylation and activation of EGFR, integrin and VEGFR, inhibited the EGF induced signaling of MAPK and ERK, PI3K and Akt and Rac1 mediated pathways, and diminished the EGF induced expression of proliferation and migration related proteins."
27589842
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"In accordance with this, the Akt and ERK pathways are less activated by EGF in those cells."
26830684
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"Through this effect WNK2 depletion potentiates the activation of ERK1/2 by EGF and promotes G1/S cell cycle progression [33]."
18593598
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"Though EGF induced ERK activation in the SHPS-1 mutant cells is similar to that in wild-type cells, IGF-1-induced ERK activation in the mutant cells is enhanced [62]."
12023008
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"Firstly, RNAi studies by others XREF_BIBR, XREF_BIBR and our experiments (XREF_FIG and XREF_FIG) demonstrated that Shoc2 has significant role in EGF induced ERK1/2 activation only when mammalian cultured cells, such as HeLa, HEK293 and Cos1, are stimulated with low, physiological concentrations of EGF (< 1 ng/ml)."
22606262
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"To gain an insight into the function of growth factors and inflammatory factors in IBDC metastasis, the representative growth factor, EGF, which can activate ERK1/2, and one of the most common inflammatory factors, IL-1beta, were investigated."
23083134
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"Fig. 3 A shows that EGF induced activation of ERK1/2 was further attenuated in cells co-transfecting p85 with Spred2 compared to cells transfecting with Spred2 or p85 alone, suggesting that the format[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
22305891
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"Using specific chemical inhibitors, we demonstrated that EGF induced EMT is mediated by extracellular signal regulated kinase 1/2 (ERK 1/2) signalling pathway and that resveratrol is able to repress EGF induced ERK activation."
21794976
reach
"Since EGF activation of ERKs are independent of the PI 3-kinase pathway in NIH (M17) cells, our results indicate that the MEK-ERK- and the PI 3-kinase-dependent pathways constitute two independent sig[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
11377817
reach
"Previous work with PC12 cells showed that epidermal growth factor (EGF) induces transient activation of extracellular signal regulated kinase (ERK) and results in cell proliferation, while sustained ERK activation triggered by nerve growth factor (NGF) leads to cell differentiation [XREF_BIBR, XREF_BIBR]."
22710166
sparser
"For instance, epidermal growth factor-induced transient ERK activation via the Raf-MEK-ERK axis induces proliferation in PC12 neuroendocrine cells, whereas nerve growth factor-induced prolonged ERK activation via the Raf-MEK-ERK axis leads to the differentiation of PC12 cells into sympathetic neuron-like cells [ xref , xref ]."
30275408
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"In addition, activation of extracellular signal regulated kinase by EGF treatment was reduced by flagellin pre-treatment."
25542757
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"Both the experimental data and simulations showed that the activation of the Ras and ERK and PI3K and AKT pathways by persistent EGF or insulin stimulation was transient (XREF_FIG and XREF_SUPPLEMENTARY)."
19357636
sparser
"On the other hand, EGF activated ERK at the same time, which could target MMP9 directly to promote the migration ( xref )."
32174831
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"We also tested whether the Src-kinase inhibitor could block the EGF induced ERK1/2 activation in the cells."
27930693
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"GnRH-, PMA- and EGF induced a robust ERK1/2 activation within 5 min of stimulation in both alphaT3-1 (XREF_FIG) and LbetaT2 cells (XREF_FIG)."
26238084
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"In 3D cell cultures and living mice, we discovered that local increases in ECM stiffness increase basal and EGF-driven activities of Akt and ERK in two different triple-negative breast cancer cell lines."
35106975
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"Mitogenesis in response to EGF can not be studied in COS-7 cells as they are partially transformed, but it is known that picomolar EGF doses are able to activate the Ras and extracellular signal regulated kinase (ERK) signaling cascade, the central driver of cell proliferation in a PI3K dependent mode, in this cell type XREF_BIBR."
25265278
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"On the other hand, the EGF treatment adopted by Albeck and Brugge stimulates not only ERK, but also other signaling molecules, such as PI3K and Akt."
24140422
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"Indeed, ERK1/2 siRNA suppressed the ability of EGF and IL-1beta to induce activation of an AP-1 reporter gene, which demonstrated that the EGF and IL-1beta-induced activation of ERK1/2 led to the upregulation of MMP-9 by increasing the transcriptional activity of AP-1."
23083134