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PAN2 binds SFXN1. 27 / 27
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"Peritoneal dissemination of cancer cells to the peritoneum, mesenterium, diaphragm, and others, was observed in a large proportion of the mice inoculated with TCC-Pan2 cells."
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"To test this, we pre-treated PK-8 and TCC-Pan2 cells with the mitochondrial-specific ROS scavenger Mito-TEMPO [ xref ] for 3 h prior to HSP70 inhibition with AP-4-139B. Interestingly, we found that pre-treatment of PDAC cells with Mito-TEMPO significantly suppressed the ability of AP-4-139B to induce AMPK phosphorylation at threonine 172 (Fig.  xref )."
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"To evaluate the potential for compensation of transferrin-mediated iron uptake to rescue proliferation, we tested TCC-Pan2 NCOA4 KD cells for rescue by holo-transferrin, demonstrating a partial rescue ( xref )."
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"Intriguingly, LCN2 levels were also significantly elevated in TCC-Pan2 NCOA4 KD proteomes (Supplementary Fig. S5E)."
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"After OI of TCC-Pan2 cells, peritoneal metastasis and ascites formation occurred in 66.6% of the host mice (Table xref )."
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"Therefore, we evaluated FPN expression by immunoblot (not detected in proteome) and demonstrated a decrease in expression in TCC-Pan2 and PANC-1 NCOA4 KD cells ( xref )."
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"The xenografted PCa models have been studied using colon cancer (HCT-116, LS174T, HT29, SW480, SW620, LoVo, RKO, Caco-2, KM12, MDST8, HUTU80) [ xref , xref , xref , xref , xref ], pancreatic cancer (Panc-1, TCC-Pan2, BxPC3, AsPC-1) [ xref , xref , xref ], gastric cancer (60As6, HSC-44PE, HSC-58, MKN45-P) [ xref , xref , xref , xref , xref , xref , xref , xref , xref , xref , xref , xref , xref , xref ], and ovarian cancer (OVCAR3, OVCAR4, OVCAR5, OVCAR8, CAOV3, OVSAHO, OV2944-HM-1, SKOV-3) [ xref , xref , xref , xref ] cell lines."
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"TCC-Pan2 cell line was characterized for growth rate, tumor markers, histology, and somatic mutations."
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"TCC-Pan2 cells were implanted orthotopically to produce PD."
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"TCC-Pan2 cells from these metastatic foci were expanded in vitro and then implanted orthotopically in mice."
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"Orthotopically implanted TCC-Pan2 cells caused tumor formation and PD with high frequency in mice."
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"TCC-Pan2 cell line and Pan2MmLuc PD model can serve as useful tools for monitoring the responses to antineoplastic agents and for studying PDAC biology."
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"Here, we describe the characterization of a human PDAC cell line, TCC-Pan2, and report the development of an orthotopic PD mouse model (Pan2MmLuc)."
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"In this study, we characterized a human PDAC cell line (TCC-Pan2) from the metastatic ascitic tumor of a Japanese patient."
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"TCC-Pan2 cells were morphologically spherical and floated freely, and adherent cells exhibited the typical morphologic features of epithelial cells (Fig. xref A)."
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"TCC-Pan2 cells secreted duke pancreatic monoclonal antigen type 2 (DUPAN-2; mean [SD], 93.2 [41.9] U/mL) and s-pancreas-1 antigen (SPan-1; 52.0 [25.2] U/mL), but we detected no carcinoembryonic antigen and carbohydrate antigen production."
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"In STR genotyping, the DNA extracted from TCC-Pan2 cell line did not correspond to that of any cells in the database of the Japanese Collection of Research Bioresources (JCRB; database of 2279 cells registered in the American Type Culture Collection, the Deutsche Sammlung von Mikroorganismen und Zellkulturen, and the JCRB)."
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"We performed NGS analyses on the ascitic tumor of the patient and on TCC-Pan2 and Pan2MmLuc cell lines by using the NCC oncopanel (v4) for 114 cancer-related genes (Supplementary Table 1). xref KRAS mutation was identified at codon 12 (Gly to Arg, G12R), and TP53 splicing mutation was observed in both alleles."
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"Pathological Characterization of the OI Model in Nude Mice Generated Using the Established TCC-Pan2 Cancer Cell Line."
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"When injected intrapancreatically, cultured TCC-Pan2 cells survived and showed high tumorigenicity (100%)."
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"The histology of the engrafted tumor of the TCC-Pan2 cell line revealed a poorly differentiated adenocarcinoma (Fig. xref B), resembling what was found in the original histological typing."
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"The tumor established using TCC-Pan2 cells exhibited a rich stroma and further showed stromal hyperplasia at the OI site (Fig. xref C)."
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"We confirmed the identity of the isolated cell line through STR analysis performed to compare specific regions of the DNA from the Pan2MmLuc subline and the TCC-Pan2 parental cell line."
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"Although PDAC frequently metastasizes to the regional lymph nodes, liver, and peritoneum in the early stages of the cancer, only a few cell lines have been reported to metastasize spontaneously in vivo into regional lymph nodes or distant organs such as the liver, lung, and peritoneum in immunodeficient mouse SC implantation models. xref , xref In this study, we characterized a previously established cell line, TCC-Pan2, derived from a PDAC (moderately/poorly differentiated adenocarcinoma), and it was found to exhibit the potential for spontaneous metastasis and PD after SC implantation or OI in nude mice."
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"TCC-Pan2 cells were positive for pancreatic tumor markers (DUPAN-2 and SPan-1) and were identified as being derived from PDAC, and the histology of the transplanted tumors resembled that of poorly differentiated adenocarcinoma."
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"Activation of the oncogene KRAS and inactivation of the tumor suppressor gene TP53 have been proposed to play a pivotal role in PDAC progression. xref , xref Pancreatic ductal adenocarcinoma cell lines have been considered to poorly represent the tumor of origin. xref However, mutation of KRAS and TP53 , gene amplification of KRAS , and homozygous deletion of CDKN2A were present in the original ascitic tumor and in TCC-Pan2 and metastatic Pan2MmLuc cell lines (Table xref )."
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"For the isolation of a PD cell line, TCC-Pan2 was selected because of its aggressiveness and because of metastasis development in the liver and lymph nodes and PD in the OI model."
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