IndraLab

Statements

USP17L2 is phosphorylated. 15 / 15
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sparser
"It was also shown that palbociclib did not directly interact with SNAIL1, but instead acted through the phosphorylation of deubiquitinating enzyme 3 (DUB3), which, in turn, downregulates SNAIL1, xref suggesting a new target for palbociclib."
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rlimsp
"These results establish that CDK4/6-mediated phosphorylation of DUB3 is important for DUB3 activity and SNAIL1 stability."
28067227
sparser
"In particular, it has been demonstrated that both these CDKs can bind and phosphorylate the deubiquitinase USP51 and DUB3, to control ZEB1 [ xref ] and SNAIL1 [ xref ] expression, respectively, thereby modulating the metastatic phenotype of cancer cells."
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sparser
"Interestingly, CDK1 also phosphorylated DUB3 at Ser41 in vitro and the treatment of Roscovitine could decrease the phosphorylation of DUB3 ( xref )."
28067227
sparser
"In agreement with the recent report that the deubiquitination activity of DUB3 relies on CDK4/6-mediated phosphorylation of DUB3 and this activity is inhibited by the CDK4/6 inhibitor ( xref ), we demonstrated that treatment of mice with the CDK4/6 inhibitor palbociclib largely sensitized DUB3-proficient prostate tumors to JQ1, but the effect of palbociclib was almost completely abolished by DUB3 knockdown."
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sparser
"However, S41A mutation completely abrogated the phosphorylation of DUB3 at this site, indicating the specificity of this antibody."
28067227
rlimsp
"To further identify the potential phosphorylation sites in DUB3, we generated MDA-MB-231 cells stably expressing FLAG-DUB3 to perform tandem affinity purification and mass spectrometry analysis of potential phosphorylation events in DUB3."
28067227
sparser
"We next tested CDK4/6-mediated phosphorylation of DUB3 in cells."
28067227
sparser
"In agreement with the recent report that the deubiquitination activity of DUB3 relies on CDK4/6-mediated phosphorylation of DUB3 and that this activity is inhibited by the CDK4/6 inhibitor ( Liu et al[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
30057199
sparser
"Furthermore, depletion of CDK4 or CDK6 in cells only partially decreased the phosphorylation of DUB3, while double knockdown of CDK4 and CDK6 almost completely abrogated DUB3 phosphorylation ( xref )."
28067227
sparser
"As shown in xref , WT DUB3 was phosphorylated in cells."
28067227
rlimsp
"Furthermore, depletion of CDK4 or CDK6 in cells only partially decreased the phosphorylation of DUB3, while double knockdown of CDK4 and CDK6 almost completely abrogated DUB3 phosphorylation (Fig. 5g)."
28067227
sparser
"These results establish that CDK4/6-mediated phosphorylation of DUB3 is important for DUB3 activity and SNAIL1 stability."
28067227
sparser
"CDK4/6 phosphorylates and activates DUB3."
28067227
sparser
"CDK4 also regulates BRD4 protein levels via phosphorylating SPOP and DUB3 that play a role in BRD4 ubiquitination and degradation ( xref ; xref ; xref ; xref )."
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