IndraLab

Statements

1,4-dithiothreitol inhibits KCNMA1. 6 / 6
| 1 5
reach
"SLO was reduced by 10 mM DTT 5 min at room temperature before dilution in Ca -free HBSS (200 U ml ) and added on target cells for 10 min at 37 °C. Cells were then washed once in complete medium before adding biotin–phenol medium."
34257406
reach
"Our results show that DTT alone does not reverse the increased Slo channel current, but that MSRA applied concurrently with DTT partially reverses the increased current induced by Ch-T or spontaneous run-up, suggesting that oxidation of methionine to met (O) is involved."
11222629
reach
"The observation that the decreased Slo channel current induced by patch excision and H 2 O 2 is restored by DTT implicates cysteine oxidation as the underlying mechanism."
11222629
eidos
"SLO was reduced by 10 mM DTT 5 min at room temperature before dilution in Ca2 + - free HBSS ( 200 U ml-1 ) and added on target cells for 10 min at 37 degreesC ."
34257406
reach
"It has been proved that DTT inhibits the activity of the plasmalemmal BK channel, which means that under reduced conditions the potassium current does not flow through the channel [65]."
36290615
reach
"Similarly, DTNB, MTSEA, and PCMB, cysteine specific reagents, decreased hSlo currents and their effects were reversed by DTT."
11222629