IndraLab

Statements

1,4-dithiothreitol inhibits KCNMA1. 6 / 6
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"SLO was reduced by 10 mM DTT 5 min at room temperature before dilution in Ca2+-free HBSS (200 U ml−1) and added on target cells for 10 min at 37 °C. Cells were then washed once in complete medium before adding biotin–phenol medium."
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"The observation that the decreased Slo channel current induced by patch excision and H 2 O 2 is restored by DTT implicates cysteine oxidation as the underlying mechanism."
11222629
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"SLO was reduced by 10 mM DTT 5 min at room temperature before dilution in Ca -free HBSS (200 U ml ) and added on target cells for 10 min at 37 °C. Cells were then washed once in complete medium before adding biotin–phenol medium."
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"Similarly, DTNB, MTSEA, and PCMB, cysteine specific reagents, decreased hSlo currents and their effects were reversed by DTT."
11222629
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"Our results show that DTT alone does not reverse the increased Slo channel current, but that MSRA applied concurrently with DTT partially reverses the increased current induced by Ch-T or spontaneous run-up, suggesting that oxidation of methionine to met (O) is involved."
11222629
eidos
"SLO was reduced by 10 mM DTT 5 min at room temperature before dilution in Ca2 + - free HBSS ( 200 U ml-1 ) and added on target cells for 10 min at 37 degreesC ."
34257406