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IKK_complex phosphorylates CYLD. 43 / 43
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"IKK family kinases phosphorylate CYLD in the ATLL MT4 cell line."
32024820
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"Additionally, in primary adult T-cell leukemia/lymphoma (ATLL) samples and cell lines, increased IKK-induced CYLD phosphorylation was observed."
34177595
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"IKKɛ also phosphorylates and inactivates the tumor suppressor CYLD, preventing CYLD from deubiquitinating specific substrates in the NF-κB signaling pathway."
23333767
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"To examine if IKK directly phosphorylates CYLD, 0.25 mug of purified CYLD was incubated with purified IKKbeta, at a final concentration of 15 nM, in medium containing 1mM EGTA, 5 mM MgCl 2, 50 mug/uL leupeptin, 2.5 mM DTT, 1 mg/mL BSA, 6.5% glycerol, in 20 mM HEPES, pH 7.4, with or without 100 muM ATP in a final volume of 20 muL at 37degreesC for one hour."
24928390
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"Interestingly, TBK1 or IKKε specifically causes CYLD to shift to higher molecular bands, suggesting phosphorylation of CYLD by these kinases."
32215003
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"These results suggest that IKK phosphorylates CYLD at the PSD under basal condition."
24928390
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"These results suggest that IKK phosphorylates CYLD at the PSD under basal condition."
24928390
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"CYLD interacts with NEMO and is phosphorylated by IKK as a mechanism to inactivate CYLD DUB activity."
22435550
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"Therefore, IKK phosphorylates CYLD to inactivate its DUB function thus providing a window of NF-κB activation prior to signal-induced termination by A20."
21119682
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"Phosphorylation of the tumor suppressor CYLD by the breast cancer oncogene IKK."
24027431
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"The second possibility tested was phosphorylation of CYLD by IKK."
24928390
rlimsp
"As IKK-mediated phosphorylation of CYLD has been reported to negatively regulate CYLD enzymatic activity [34], we further explored whether IKK is also involved in regulating the degradation of CYLD by SCFβ-TRCP."
24961988
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"Reiley et al have shown that NEMO is required for the IKK complex to phosphorylate and inhibit the deubiquitinase CYLD [ xref ], a critical component of the necrotic machinery [ xref ], as can the TNF-inducible IKKε [ xref ]."
21232018
rlimsp
"Although this identified phospho-degron motif (GSIGHS) within CYLD is not consistent with the canonical β-TRCP degron (DSGXXS), it does resemble a reported non-canonical degron found within p53 that also contains IKK phosphorylation sites, and other known β-TRCP substrates including Mcl-1, PHLPP1 and Gli2 (Supplementary Figure 3A) [35-38]."
24961988
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"To investigate further if IKK kinases are involved in phosphorylating CYLD in MT4 cells, we examined the effect of MRT67307 (TBK1/IKKɛ inhibitor) and TPCA (IKKβ inhibitor)."
32024820
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"IKK, a Ca 2+ -independent kinase, known to phosphorylate CYLD at S-418 in non neuronal cells [XREF_BIBR], [XREF_BIBR], is present in the PSD fractions [XREF_BIBR]."
24928390
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"39 Interestingly, the phosphorylation of CYLD is mediated by IKK, 39 implying that CYLD may not be a major factor involved in feedback control of NF-kappaB activation."
19373246
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"The following are some well-known and extensively studied examples.Phosphorylation of CYLD by IKKε promotes cell transformation by increasing NF-κB activation [104], but the presence of the IKK inhibitors leads to apoptosis indicating that CYLD may be a novel therapeutic target for adult T-cell leukemia [31]."
32512887
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"Phosphorylation of the tumor suppressor CYLD by the breast cancer oncogene IKK. promotes cell transformation."
24027431
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"However, constitutive phosphorylation of CYLD by IKK inactivates CYLD in HTLV-1-induced leukemia to promote heightened NF-kappaB activation."
22435550
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"IkappaB kinase (IKK), with regulatory subunit IKKgamma, phosphorylates CYLD and results in the loss of its deubiquitinating function towards TRAF2."
23797609
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"Interestingly, phosphorylation of CYLD by IKK inhibits its DUB activity ( xref )."
30524423
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"Activation of IKK in isolated PSDs also promoted phosphorylation of CYLD and an increase in endogenous deubiquitinase activity for K63 linked polyubiquitins."
24928390
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"Regarding the latter possibility, a recent study suggests that CYLD phosphorylation can also be mediated by the IKK related kinase IKKepsilon and contributes to IKKepsilon induced tumorigenesis [XREF_BIBR]."
21824392
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"Hutti et al. have reported that IKKε could also phosphorylate CYLD to inhibit its activity ."
32024820
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"Addition of ATP induced phosphorylation of CYLD at S-418 ( xref ), indicating that IKK can directly phosphorylate CYLD."
24928390
sparser
"However, constitutive phosphorylation of CYLD by IKK inactivates CYLD in HTLV-1-induced leukemia to promote heightened NF-κB activation ( xref )."
22435550
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"IkappaB kinase (IKK), with regulatory subunit IKKgamma, phosphorylates CYLD and results in the loss of its deubiquitinating function towards TRAF2."
23797609
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"Furthermore, the IKK complex that phosphorylates CYLD is also necessary for the induction of NF-κB-dependent transcription."
35109788
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"In HTLV-1 infected cells CYLD is constitutively phosphorylated by IKK, which impairs its function thus allowing persistent NF-kappaB activation [XREF_BIBR, XREF_BIBR]."
25341660
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"CYLD interacts with NEMO and is phosphorylated by IKK as a mechanism to inactivate CYLD DUB activity ( xref )."
22435550
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"We now report that phosphorylation of CYLD by IKK family kinases in HTLV-1 transformed T cells inhibits RIPK1 from activating the cell death pathway and inhibiting these kinases reactivates CYLD and RIPK1-dependent tumor cell death."
32024820
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"Therefore, in order to determine which IKK family member (s) can most efficiently phosphorylate CYLD, we cotransfected Myc-CYLD with individual IKK family members fused to GST."
19481526
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"Addition of ATP induced phosphorylation of CYLD at S-418 (XREF_FIG), indicating that IKK can directly phosphorylate CYLD."
24928390
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"In HTLV-1 infected cells CYLD is constitutively phosphorylated by IKK, which impairs its function thus allowing persistent NF-κB activation [ xref , xref ]."
25341660
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"Activation of IKK in isolated PSDs also promoted phosphorylation of CYLD and an increase in endogenous deubiquitinase activity specific for K63 linked polyubiquitins."
24928390
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"To ensure that IKK could directly phosphorylate CYLD rather than via sequential phosphorylation through other PSD-associated kinases, purified CYLD and purified IKKβ (catalytic subunit) were co-incubated in the presence or the absence of ATP."
24928390
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"The second possibility tested was phosphorylation of CYLD by IKK."
24928390
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"Seminal work by Sun and colleagues showed that CYLD was phosphorylated by IKKβ and this inhibited its catalytic activity ."
32024820
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"Both of the IKK sub-families can phosphorylate CYLD, and the combination of MRT67307 and TPCA have a marked effect in reducing CYLD phosphorylation and triggering cell death."
32024820
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"To ensure that IKK could directly phosphorylate CYLD rather than via sequential phosphorylation through other PSD associated kinases, purified CYLD and purified IKKbeta (catalytic subunit) were co-incubated in the presence or the absence of ATP."
24928390
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"These observations indicate that endogenous IKK activity in isolated PSDs mediates CYLD phosphorylation in the absence of Ca 2+."
24928390
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"Interestingly, the CYLD phosphorylation requires IkappaB kinase gamma (IKKgamma) and can be induced by IKK catalytic subunits."
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