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JAK2 phosphorylates STAT3 on Y705. 84 / 140
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"To examine whether STAT3 activation has any influence on HBV infection or on efficiency of HBV replication, we inhibited STAT3 phosphorylation with AG-490, a JAK-2 protein tyrosine kinase inhibitor that inhibits Y705 phosphorylation of STAT3."

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"Binding of leptin to its receptor in AGRP neurons activates JAK2 which phosphorylates STAT3 at Tyr705 (p-STAT3); p-STAT3 then migrates as a dimer to the nucleus, where it inhibits transcription of both Agrp and Npy ( xref )."

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"In response to cytokine stimulation, STAT3 is phosphorylated on tyrosine 705 by the JAK2 and TYK2 kinase, resulting in its dissociation from the cytoplasmic tail of cytokine receptors and dimerization[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"The activated IL-6/IL-6 receptor/gp130 complex now recruits Janus kinase 2 (JAK2), which directly phosphorylates STAT3 at tyrosine 705 (Tyr705)."

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"In response to external stimuli, STAT3 is phosphorylated by JAK2 at tyrosine 705 (Tyr705), leading to its translocation from the cytoplasm to the nucleus, which promotes the transcription of various hypertrophic factors, including natriuretic peptide type A (Nppa) and natriuretic peptide type B (Nppb) [18]."

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"These results indicate that JAK2 mediates the STAT3 Y705 phosphorylation in HPV18-containing primary keratinocytes."

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"Phosphorylation of STAT3 at tyrosine 705 (Y705) by JAK2 is one of the most important and well established cytoplasmic functions of JAK2 xref , xref ."

sparser
"In bone metastatic prostate cancer, IHC analyses have identified that the majority of cases studied are positive for STAT3, and kinome profiling have shown elevated activity of JAK2, which phosphorylates STAT3 at Tyr705 resulting in head to tail dimerization, translocation to the nucleus and binding to the promoters of target survival genes such as BCL-xL and survivin xref ."

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"The phosphorylation of STAT3 at Tyr705 is most commonly mediated by Janus kinases (JAKs), especially JAK2."

sparser
"Upon leptin binding to the ob-rb receptor, Janus kinase 2 phosphorylates signal transducer and activator of transcription 3 (STAT3) at Tyr-705, which results in STAT3 dimerization and migration to the nucleus where transcription initiation is affected ( xref )."

sparser
"To determine whether JAK2 directly phosphorylates STAT3, we performed an in vitro kinase assay using kinase active JAK2 protein with STAT3 protein we purified from Sf9 insect cells and found that JAK2 phosphorylates STAT3 at Y705 ( xref )."

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"Furthermore, treatment of JAK2 clinical medicine AZD1480 to ESCC cells showed similar tendency with Lico B. Thus, JAK2 downstream signaling proteins phosphorylation of STAT3 at Y705 and S727 as well as STAT3 target protein Mcl-1 expression was decreased with treatment of Lico B. Our results suggest that Lico B inhibits ESCC cell growth, arrests cell cycle and induces apoptosis, revealing the underlying mechanism involved in JAK2/STAT3 signaling pathways after Lico B treatment."

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"Results showed that JAK2, STAT3, and MYC mRNA and protein level, as well as JAK2/STAT3/MYC axis related markers (phosphorylated JAK2, p-JAK2: Y1007&Y1008; phosphorylated STAT3, p-STAT3: Tyr705; CCND1)[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"Following ligand-induced dimerization of IL-6 receptor, the associated protein tyrosine kinases JAK1 and JAK2 are activated and subsequently induces STAT3 tyrosine-705 phosphorylation, the active form[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"IL6 binding activates the phosphorylation of a JAK (mostly JAK2), which in turn will phosphorylate STAT3 on amino acid Y705 [XREF_BIBR]."

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"Our previous reports indicated that MUC16 could induce the G2-M transition of breast cancer cells by interacting with Janus kinase 2 (JAK2), which in turn enhances the phosphorylation of STAT3 (Y705) and Aurora Kinase A [9]."

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"JAK2 kinase mediates phosphorylation of STAT3 at Y705."

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"JAK2 phosphorylates STAT3 on its Y705 residue, resulting in the dimerization and nuclear translocation of STAT3 and activation of STAT3 transcriptional activity, leading to the expression of STAT3 target genes that promote cell proliferation, differentiation, survival, and migration [ xref , xref , xref ]."

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"JAK2 phosphorylates STAT3 at Y705 permitting subsequent p-STAT3 homodimerization through interaction of their phosphorylated Y705 site and SH2 domain to induce its nuclear translocation and transcriptional activity [XREF_BIBR, XREF_BIBR]."

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"Once activated by stimuli, EGFR activates Janus kinase 2 (JAK2) to phosphorylate STAT3 on residue Tyr705 [43,44] ."

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"IL-6 triggers JAK2-dependent phosphorylation of STAT3 Y705, which facilitates STAT3 dimerization and translocation into nucleus to initiate the transcription of a wide spectrum of genes 8. Indeed, knockdown of STAT3 largely abolished Glut5 expression in the IL-6-treated DU145 and HSC-3 cells (Figure 2D)."

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"To this end, we began by testing the effect of HME on the activation status of JAK2 and SRC, two eminent STAT3 upstream kinases that phosphorylate STAT3′s tyrosine 705 to induce STAT3 activation [10]."

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"STAT3 is subsequently phosphorylated by Jak2 on Y705 on the C-terminus, which leads to the formation of STAT3 dimers via reciprocal interactions between the SH 2 domain and phosphorylated Y705."

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"However, acetylated STAT1 promotes STAT1 binding with STAT3 to form a heterodimer in the cytoplasm, which prevents STAT3 transport into the nucleus even though JAK2 phosphorylates STAT3 at Y705."

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"Upon cytokine receptor and gp130 receptor activation, STAT3 phosphorylation at Tyr 705 is mediated by JAK1 or JAK2."

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"The proximity of JAK2 and STAT3 promotes the phosphorylation of STAT3 at tyrosine-705 (Y705) initiating the translocation of STAT3 to the nucleus, where it functions as a transcription factor, initiating the gene expression of the genes involved in tumourigenesis (Figure 2) [38,39]."

sparser
"JAK2 phosphorylates STAT3 at the Y705 residue, which facilitates STAT3 dimerization, nuclear translocation, and activation of its transcriptional activity, thereby promoting the expression of target genes associated with cell proliferation, differentiation, survival, and migration [ xref , xref ]."

sparser
"STAT3 is phosphorylated by JAK2 on Y705 to allow for STAT3 dimerization and translocation to the nucleus to increase Pomc expression and inhibit Agrp expression [28,29] ."

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"In bone metastatic prostate cancer, IHC analyses have identified that the majority of cases studied are positive for STAT3, and kinome profiling have shown elevated activity of JAK2, which phosphorylates STAT3 at Tyr705 resulting in head to tail dimerization, translocation to the nucleus and binding to the promoters of target survival genes such as BCL-xL and survivin 59."

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"On the other hand, WP1066 inhibits JAK2-trigged phosphorylation of STAT3 (Tyr 705), and blocks its nuclear translocation, thus repressing transcription of pro-survival BCL-2, BCL-XL and MCL-1."

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"STAT3 is recruited to the LEPR-B and is phosphorylated by JAK2 on Tyr 705, allowing for STAT3 dimerisation and translocation to the nucleus to mediate gene transcription."

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"JAK2 phosphorylates STAT3 at Tyr 705 in response to leptin, which is required for STAT3 activation."

sparser
"Once secreted, IL-6 binds to its cognate receptor and activates a tyrosine kinase JAK2 which phosphorylates STAT3 at Y705, a modification that promotes STAT3 nuclear translocation, therefore forming a[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"These findings suggest that STAT3 interacting with DNMT1 is phosphorylated at Y705 by JAK2 in melanoma and breast cancer cells."

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"In agreement with our study, S-glutathionylation at Cys542 was found to impair JAK2-induced Tyr705 phosphorylation of STAT3 [ 94 ]."

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"Once activated by Jak2, Stat3 is phosphorylated on tyrosine 705 (Tyr705) and serine 727 (Ser727), and the phosphorylation of both amino acid species is required for full Stat3 activity ( Aggarwal et a[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"Gp130 activation then induces the phosphorylation of JAK kinase 1 (JAK1) and JAK2, which in turn phosphorylate STAT3 on tyrosine-705, triggering STAT3 homodimerization, nuclear translocation, DNA bind[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"As JAK2 directly phosphorylates STAT3 at Tyr705, we next examined the possible interactions of DLEC1 with STAT3 and JAK2 by reciprocal co-immunoprecipitation (co-IP) experiments with Flag or DLEC1, STAT3 and JAK2 antibodies in carcinoma (KYSE150, H1299) and immortalized cells (HEK293T)."

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"Non‐receptor tyrosine kinase JAK2 phosphorylated the STAT3 at Tyr705 to activate STAT3 activity, then causing STAT3 dimerization and translocation to the nucleus to induce transcription of target genes. [ xref ] Therefore, JAK2 was indispensable for STAT3 activation."

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"The JAK2 and c-Src kinase in turn phosphorylate STAT3 at tyrosine 705, leading to STAT3 dimer or formation and translocation into the nucleus [XREF_BIBR]."

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"Our results identify STAT3 as essential for mediating the acute effects of leptin not only on food intake but also on gonadotropin secretion and on hepatic glucose fluxes.Upon leptin receptor activati[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"In response to external stimuli, STAT3 is phosphorylated by JAK2 at tyrosine 705 (Tyr705), leading to its translocation from the cytoplasm to the nucleus, which promotes the transcription of various hypertrophic factors, including natriuretic peptide type A ( Nppa ) and natriuretic peptide type B ( Nppb ) [ xref ]."

sparser
"Gp130 activation induces the phosphorylation of JAK kinase 1 (JAK1) and JAK2, which in turn phosphorylate STAT3 at Y705, triggering its homodimerization, nuclear translocation, DNA binding and the transactivation of pro-inflammatory STAT3 target genes. xref "

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"Moreover, evidence has shown that phosphorylation of STAT3 at Tyr-705 by JAK2 activates its transcriptional activity, whereas phosphorylation of Ser-727 by ERK1/2 maximizes transcriptional ability of [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"Conditioned medium of ADSC activated the downstream JAK1 and JAK2 and TYK2 that lead to their target STAT3 Tyr 705 phosphorylation in rnCM and HL-1 cardiomyocytes."

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"Of note, JAK2 phosphorylates STAT3 at tyrosine 705, which in turn upregulates STAT3 gene expression [31,60]."

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"To determine whether JAK2 directly phosphorylates STAT3, we performed an in vitro kinase assay using kinase active JAK2 protein with STAT3 protein we purified from Sf9 insect cells and found that JAK2 phosphorylates STAT3 at Y705 (Fig. 5B)."

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"Phosphorylation of STAT3 at tyrosine 705 (Y705) by JAK2 is one of the most important and well established cytoplasmic functions of JAK2 XREF_BIBR, XREF_BIBR."

sparser
"Of note, JAK2 phosphorylates STAT3 at tyrosine 705, which in turn upregulates STAT3 gene expression [ xref , xref ]."

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"Upon IL6 or LIF ligand binding to cognate receptors, the associated Janus Kinase 2 (JAK2) phosphorylates STAT3 on tyrosine 705 thereby promoting stem gene expression."

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"JAK2 phosphorylates STAT3 at Y705 to activate its nuclear import and transcriptional activity xref , xref ."

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"Also, JAK2 phosphorylates STAT3 at Tyr705, inducing its dimerization and translocation to the nucleus where it regulates the expression of different proteins involved in cancer progression, such as cyclin D1, COX2, VEGF, and SOCS3, a negative regulator of leptin signaling [ xref , xref ]."

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"JAK1 and JAK2 phosphorylate STAT3 at Tyr705, resulting in the translocation of activated STAT3 dimers to the nucleus."

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"In response to stressful conditions, STAT3 is phosphorylated at tyrosine 705 by JAK2 and TYK2 kinase, causing the dimerization of STAT3 and its translocation to the nucleus."

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"The Janus family of tyrosine kinase 2 (JAK2), an upstream kinase, mediates STAT3 phosphorylation at Tyr 705 site."

sparser
"However, acetylated STAT1 promotes STAT1 binding with STAT3 to form a heterodimer in the cytoplasm, which prevents STAT3 transport into the nucleus even though JAK2 phosphorylates STAT3 at Y705."

sparser
"Finally, the JAK2 kinase may induce phosphorylation of Tyr705 on the STAT3 that bound with the receptor, and then the activated STAT3 would enter the nucleus in a form of a dimer to bind specifically with the DNA sequences to trigger the expression of downstream target genes such as cyclin D1 , c-myc , c-Jun , bcl , bcl-xL , and mcl-I . These genes were reported to modulate the cell cycle and inhibit the cell apoptosis, which may be participated in the protective effects of vascular endothelial barrier function [ xref – xref ]."

sparser
"As JAK2 directly phosphorylates STAT3 at Tyr705, we next examined the possible interactions of DLEC1 with STAT3 and JAK2 by reciprocal co-immunoprecipitation (co-IP) experiments with Flag or DLEC1, STAT3 and JAK2 antibodies in carcinoma (KYSE150, H1299) and immortalized cells (HEK293T)."

sparser
"It also contains constitutively activated STAT3 phosphorylated by JAK2 kinase at the site of Tyr705 ( xref ) and activated telomerase."

sparser
"JAK1 and JAK2 phosphorylate STAT3 at Tyr705, resulting in the translocation of activated STAT3 dimers to the nucleus."

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"The phosphorylation of STAT3 at Tyr 705 is most commonly mediated by Janus kinases (Jaks), especially Jak2 XREF_BIBR."

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"JAK2 phosphorylates STAT3 at Y705 to activate its nuclear import and transcriptional activity XREF_BIBR, XREF_BIBR."

sparser
"Finally, the JAK2 kinase may induce phosphorylation of Tyr705 on the STAT3 that bound with the receptor, and then the activated STAT3 would enter the nucleus in a form of a dimer to bind specifically with the DNA sequences to trigger the expression of downstream target genes such as cyclin D1, c-myc, c-Jun, bcl, bcl-xL, and mcl-I. These genes were reported to modulate the cell cycle and inhibit the cell apoptosis, which may participate in the protective effects of vascular endothelial barrier function [35–37]."

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"The phosphorylation of STAT3 at Tyr 705 is most commonly mediated by Jaks, especially Jak2 XREF_BIBR."

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"Therefore, we validated that C21orf58 modulated the cell growth by activating STAT3 signaling.Non‐receptor tyrosine kinase JAK2 phosphorylated the STAT3 at Tyr705 to activate STAT3 activity, then causing STAT3 dimerization and translocation to the nucleus to induce transcription of target genes."

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"STAT3 phosphorylation at Tyr705 is catalyzed by Jak1 or Jak2 kinases and both are activated by binding to CK2 [XREF_BIBR]."

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"Gp130 activation induces the phosphorylation of JAK kinase 1 (JAK1) and JAK2, which in turn phosphorylate STAT3 at Y705, triggering its homodimerization, nuclear translocation, DNA binding and the transactivation of pro inflammatory STAT3 target genes."

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"JAK2 phosphorylates STAT3 at Tyr 705 in response to leptin, which is required for STAT3 activation."

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"Upon receptor activation, STAT3 phosphorylation (at tyrosine 705) is mediated by Janus kinases, JAK1 or JAK2."

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"STAT3 is activated when the signal transduction protein janus kinase 2 (JAK2) is stimulated by any of a variety of cytokines (IL-6, IL-11) and growth factors (EGF, TGF-alpha, PDGF and HGF) to phosphorylate the STAT3 tyrosine 705 residue [XREF_BIBR, XREF_BIBR]."

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"In response to the cytokine stimulation, STAT3 is phosphorylated on tyrosine 705 by the JAK2/TYK2 kinase, results in its dissociation from the cytoplasmic tail of cytokine receptors and to form a dime[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"S glutathionylation of C328 and C542 within the DNA binding and linker domains, respectively, were shown to block JAK2 mediated phosphorylation of recombinant STAT3 Y705 likely due to steric or conformational reasons."

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"Moreover, evidence has shown that phosphorylation of STAT3 at Tyr 705 by JAK2 activates its transcriptional activity, whereas phosphorylation of Ser 727 by ERK1/2 maximizes transcriptional ability of [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"JAK2, an upstream kinase, recruits STAT3 to its phosphorylation site and mediates STAT3 phosphorylation at Tyr-705, which results in dimerization, nuclear translocation of STAT3, and ultimately initia[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"Upon leptin binding to the ob-rb receptor, Janus kinase 2 phosphorylates signal transducer and activator of transcription 3 (STAT3) at Tyr 705, which results in STAT3 dimerization and migration to the nucleus where transcription initiation is affected."

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"JAK2 is the most commonly reported kinase to phosphorylate STAT3 at tyrosine 705 in breast cancer cell lines."

sparser
"The JAK2 and c-Src kinase in turn phosphorylate STAT3 at tyrosine 705, leading to STAT3 dimer or formation and translocation into the nucleus [ xref ]."

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"These findings suggest that STAT3 interacting with DNMT1 is phosphorylated at Y705 by JAK2 in melanoma and breast cancer cells."

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"NO can restrain the phosphorylation of STAT3 (Tyr 705 ) mediated by JAK2 to inhibit the transactivation of STAT3 and the expression of down-stream gene expressions in microglia inflammation via S-nitr[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"32, 33 In the pathogenesis of cancer, elevated IL-6 and IL-27 could stimulate the activation of JAK1 and JAK2 enzymes, which subsequently mediate tyrosine phosphorylation of STAT3 at Tyr705."

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"JAK2 phosphorylates STAT3 at the Y705 residue, which facilitates STAT3 dimerization, nuclear translocation, and activation of its transcriptional activity, thereby promoting the expression of target genes associated with cell proliferation, differentiation, survival, and migration [73, 74]."

sparser
"JAK2 phosphorylates STAT3 at Y705 permitting subsequent p-STAT3 homodimerization through interaction of their phosphorylated Y705 site and SH2 domain to induce its nuclear translocation and transcriptional activity [ xref , xref ]."

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"The phosphorylation of STAT3 at Tyr705 and Ser727 is mediated by the receptor-associated tyrosine kinase JAK2, and both molecules are dephosphorylated by AUY-922 [32]."

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"It is currently well characterized that the inflammatory induction of hepcidin expression is mainly mediated by IL-6, which binds to the IL-6 receptor and gp130 and then recruits Janus kinase 2 (JAK2) to phosphorylate STAT3 at tyrosine residue 705 [XREF_BIBR, XREF_BIBR]."