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CALM1 binds CALM. 74 / 74
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"NMR spectral assignments for the CaM/CaM1 complex were reported previously (BMRB accession number 51222)."
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"The NMR-derived structure of CaM/CaM1 reveals intermolecular contacts with the CaM1 residue, F575, that points toward the Ca -occupied N-lobe of CaM bound to CaM1 (Figure 4B)."
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"The ITC results indicate that CaM2 binds to the CaM C-lobe with higher affinity than the CaM N-lobe, in contrast to CaM1 that binds to the CaM N-lobe with higher affinity."
36070238
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"The PDE1 activation process involves Ca 2+ binding to the free CaM region, which results in the activation of the Ca 2+ -CAM and changes in the conformation of the PDE1 structure (Ca 2+ -CaM*-PDE1)."
33153226
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"These results imply that a single CaM may bind to CNGB1 in which the CaM N-lobe is bound to CaM1 and the CaM C-lobe is bound to CaM2."
36070238
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"It was shown that GAD activity is induced by two different mechanisms: (i) in intact plant tissue and neutral pH GAD activity is stimulated in a Ca 2+ -dependent manner by binding of calmodulin to the CAM-binding site ( xref ); (ii) after wounding of plant cells the vacuolar content is released and the cytosol is acidified which leads to a Ca 2+ -independent activation of GADs ( xref )."
28382046
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"NMR-Derived Structures of CaM Bound to the CaM1 Peptide."
36070238
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"The results of the native gel and fluorescence assays demonstrate that Fh CaM1 binds to the calmodulin binding domain of Fh PMCA."
22819963
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"The binding of CaM to the CaM1 peptide revealed a biphasic ITC isotherm in which two CaM1 molecules are bound to one CaM molecule at saturation ( xref )."
36070238
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"A recent NMR structure of CaM bound to a peptide fragment of CaM1 [ xref ] reveals that the Ca 2+ -bound CaM N-lobe binds stably to CaM1 ( xref B), but the Ca 2+ -bound CaM C-lobe binds to CaM1 with much lower affinity, which prevents determination of its structure [ xref ]."
36430626
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"Another lipid kinase, At DGK2, was shown to bind calmodulins CAM1 and CAM4 [31] ."
31295460
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"Binding of uranyl to the unphosphorylated CaM-WT and CaM1 peptides was characterized by conditional dissociation constants in the nanomolar range (K d  = 32±7 and 25±6 nM, respectively)."
22870263
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"We suggest that Ca 2+ -induced CaM N-lobe binding to CaM1 may help bridge the CLZ and CNBD to promote a Ca 2+ -induced reduction in cGMP binding to the CNG channel."
36070238
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"The CaM1 residue F575 makes extensive hydrophobic contacts with the Ca 2+ -bound CaM N-lobe ( xref B) and the mutation F575E can selectively disable the CaM N-lobe binding to CaM1 [ xref ]."
36430626
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"Current suppression occurs following Ca 2+ influx through the CNG channel, Ca 2+ then binds to calmodulin and the Ca‐CAM complex blocks the CNG channels, where it remains bound for some time."
39358841
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"The interaction between CaM1, CaM4, or CaM 7 and JAV1 depended on an increase in Ca levels at the time of injury, inducing phosphorylation of JAV1."
38611533
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"These same resonances are exchange broadened in CaM bound to the CNGB1 CaM1 peptide (Bej and Ames xref ) and the α-subunit of the retinal cyclic nucleotide-gated channel (CNGA2) (Contessa et al. xref ), but are not exchange broadened in free CaM (Bej and Ames xref ; Kainosho et al. xref )."
35986879
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"The binding of Ca -bound CaM to CaM1 was suggested to block CaM1 binding to the CLZ [7,11]."
36430626
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"The Arabidopsis genome contains seven distinct CaM genes, CaM1–CaM7, and intriguingly encodes only four protein isoforms ( McCormack et al., 2005 )."
21419340
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"By contrast, the low cytosolic Ca 2+ level in the light-activated photoreceptor should cause Ca 2+ dissociation from the CaM N-lobe, which is predicted to disable CaM N-lobe binding to CaM1 and, therefore, allow for CaM1 and CaM2 to move far apart when the channel switches to the highest open probability state ( xref A, right panel)."
36430626
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"By contrast, the low cytosolic Ca level in the light-activated photoreceptor should cause Ca dissociation from the CaM N-lobe, which is predicted to disable CaM N-lobe binding to CaM1 and, therefore, allow for CaM1 and CaM2 to move far apart when the channel switches to the highest open probability state (Figure 4A, right panel)."
36430626
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"Among seven calmodulins (CaM1–CaM7), JAV1 strongly interacts with CaM1, CaM4, and CaM7 ( A, A, and S2B)."
29625034
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"The CaM1, CaM4, CaM5, CaM6 and CaM7 interacts with MPK8 ( xref )."
30909616
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"The CaM interaction with CaM1 is not considered here because the CaM1 peptide has relatively low affinity binding to the CaM N-lobe (K = 5 μM [53]), which may be outside the physiological range of cellular-free CaM concentration (100 nM [60])."
36430626
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"The CaM interaction with CaM1 is not considered here because the CaM1 peptide has relatively low affinity binding to the CaM N-lobe (K d = 5 μM [ xref ]), which may be outside the physiological range of cellular-free CaM concentration (100 nM [ xref ])."
36430626
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"Our binding studies reveal that CaM1 binds to the Ca 2+ -bound CaM N-lobe with at least fivefold higher affinity than it binds to the CaM C-lobe."
36070238
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"We present separate NMR structures of Ca 2+ -saturated CaM bound to CaM1 and CaM2 that define key intermolecular contacts: CaM1 residue F575 interacts with the CaM N-lobe while CaM2 residues L1129, L1132, and L1136 each make close contact with the CaM C-lobe."
36070238
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"Thus, a single CaM can bind to both sites in CNGB1 in which the CaM N-lobe binds to CaM1 and the CaM C-lobe binds to CaM2."
36070238
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"In this study, we present NMR structures of CaM bound to CaM1 and CaM2 and provide an analysis of CaM binding to CaM1 and CaM2."
36070238
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"Our results suggest that a single CaM could be bound to both sites in CNGB1 in which the calcified CaM N-lobe binds preferentially to CaM1 and the calcified CaM C-lobe binds constitutively to CaM2."
36070238
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"CaM binding to CaM1 and CaM2 peptides was carried out on a VP-ITC calorimeter (Micro-Cal) at 27 °C as described previously."
36070238
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"Pf1 (10 mg/mL) was added to N-labeled CaM (0.4 mM) bound to unlabeled CaM1 (0.8 mM) to produce weak alignment."
36070238
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"The interaction of CAM1 with CaM depends on the Ca2+ occupancy of its Ca(2+)-binding sites."
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"Peptide fragments of CaM1 (residues 565–589) and CaM2 (residues 1120–1147) were shown previously to bind to Ca -bound CaM, but the binding of CaM1 and CaM2 to Ca -free CaM has not been reported."
36070238
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"Research shows that there are eight members of CBP60 protein family in Arabidopsis, including CBP60a g and systemic acquired resistance deficient 1 ( SARD1 ); SARD1 does not have a CaM-binding domain (CBD) that could bind to CAM [ xref ]."
38673934
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"By contrast, the addition of CaM1 to Ca -bound CaM caused much larger NMR spectral changes, which indicates that CaM1 binds to Ca -bound CaM with at least 100-fold higher affinity compared to that of apoCaM (see ITC binding studies below)."
36070238
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"Our binding studies reveal that CaM1 binds to the Ca 2+ -bound CaM N-lobe with at least fivefold higher affinity than it binds to the CaM C-lobe."
36070238
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"We present separate NMR structures of Ca 2+ -saturated CaM bound to CaM1 and CaM2 that define key intermolecular contacts: CaM1 residue F575 interacts with the CaM N-lobe while CaM2 residues L1129, L1132, and L1136 each make close contact with the CaM C-lobe."
36070238
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"The CNGB1 mutation F575E abolishes CaM1 binding to the CaM N-lobe while L1132E and L1136E each abolish CaM2 binding to the CaM C-lobe."
36070238
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"Removal of CaM2 did not affect CaM modulation, indicating that binding of CaM to CaM1 in the unstructured N terminus is important for modulating the channel’s activity ( xref )."
37011209
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"In this study, our binding data suggest that one CaM is bound to CNGB1 in which the CaM N-lobe binds to CaM1 and the CaM C-lobe binds separately to CaM2 ( xref )."
36070238
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"Our results suggest that a single CaM could be bound to both sites in CNGB1 in which the calcified CaM N-lobe binds preferentially to CaM1 and the calcified CaM C-lobe binds constitutively to CaM2."
36070238
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"To test this hypothesis, ITC experiments were performed to monitor CaM binding to CaM1 and CaM2 (Figure 2)."
36070238
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"The structure of the CaM N-lobe bound to CaM1 looks similar to the NMR structure of the CaM N-lobe bound to the CaM-binding site within the olfactory CNGA2 ( xref and RMSD = 1.1 Å). xref However, an important structural difference is that F575 in CaM1 (replaced by L76 in CNGA2) causes the C-terminal end of CaM1 to point downward and alters the orientation of CaM1 relative to CNGA2."
36070238
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"Biosensor experiments were performed to determine rate and equilibrium constants of the C-CAM/calmodulin interaction."
8576129
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"By contrast, the addition of CaM1 to Ca 2+ -bound CaM caused much larger NMR spectral changes, xref which indicates that CaM1 binds to Ca 2+ -bound CaM with at least 100-fold higher affinity compared to that of apoCaM (see ITC binding studies below)."
36070238
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"The binding of CaM to the CaM1 peptide revealed a biphasic ITC isotherm in which two CaM1 molecules are bound to one CaM molecule at saturation (Figure 3A)."
36070238
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"Thus, the ITC results indicate two separate CaM1 molecules bind to CaM to explain the 2:1 binding stoichiometry."
36070238
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"NMR-Derived Structures of CaM Bound to the CaM1 Peptide.."
36070238
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"It is reported that GAD is activated in one of two ways: (i) in intact plant tissue and neutral pH; GAD activity is stimulated in a Ca 2+ -dependent manner by the binding of calmodulin (CAM) to the CAM-binding site, or (ii) after wounding of plant cells, the vacuolar content is released and the cytosol is acidified, leading to a Ca 2+ -independent activation of GAD ( xref ; xref )."
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"The ITC isotherm of CaM1 binding to the CaM N-lobe exhibited an endothermic heat signal that did not saturate under the ITC conditions (Figure 7A)."
36070238
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"As a result, the NMR data could only define the structure of the CaM N-lobe bound to the CaM1 peptide."
36070238
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"Three-dimensional structures of the CaM N-lobe bound to the CaM1 peptide were calculated on the basis of the observed NOESY data ( xref ) that served as restraints for molecular docking using HADDOCK xref , xref as described in the xref section."
36070238
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"The top-ranked docked structure of the CaM N-lobe bound to CaM1 from the cluster with the lowest HADDOCK score is shown in xref , and structural statistics are provided in xref ."
36070238
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"The structure of the CaM N-lobe (cyan in xref ) bound to CaM1 (magenta helix in xref ) looks similar to the NMR structure of the CaM N-lobe bound to the olfactory CNGA2 peptide, xref and both structures overlay with an RMSD of 1.1 Å ( xref - xref )."
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"Therefore, the F575E mutation weakens the CaM N-lobe binding to CaM1 by over 10-fold and is predicted to disable CaM N-lobe binding to CNGB1 in the full-length CNG channel under physiological conditions."
36070238
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"In this study, our binding data suggest that one CaM is bound to CNGB1 in which the CaM N-lobe binds to CaM1 and the CaM C-lobe binds separately to CaM2 (Figure 3)."
36070238
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"They also promote synapse formation and stability by modulating CAM activity or interacting with CAMs (Soler-Llavina et al. xref ; Yang et al. xref )."
41201670
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"The NMR-derived structure of CaM/CaM1 reveals intermolecular contacts with the CaM1 residue, F575, that points toward the Ca 2+ -occupied N-lobe of CaM bound to CaM1 ( xref )."
36070238
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"The ITC isotherm of CaM1 F575E binding to the CaM N-lobe exhibited an endothermic heat signal that did not saturate under the ITC conditions ( xref )."
36070238
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"A nonstoichiometric exothermic heat signal was also detected for the CaM N-lobe binding to both CaM1 WT and CaM1 F575E , which is not associated with peptide binding but instead is likely an artifact caused by the sample heterogeneity of the N-lobe."
36070238
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"We also present NMR structures of CaM bound to CaM1 (Figure 4B) and CaM2 (Figure 4D)."
36070238
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"Therefore, the F575E mutation weakens the CaM N-lobe binding to CaM1 by over 10-fold and is predicted to disable CaM N-lobe binding to CNGB1 in the full-length CNG channel under physiological conditions."
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"This structural difference may explain why the CaM N-lobe binds to CaM1 with about fivefold lower affinity than it binds to CNGA2."
36070238
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"Arabidopsis calmodulin 7 (CAM7) interacts with the CAM-binding domain and the isoleucine-glutamine (IQ) domain of CNGC2 and CNGC4 which negatively regulates CNGC2/4 channel activity and flg22-induced PTI (Tian et al. xref )."
40522602
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"These data indicate that the effect of calmodulin binding to RYR CaM1 may be regulated by the phosphorylation state of the Ser residue localized within the sequence Arg-His-Arg-Val(Ile)-Ser-Leu."
7711031
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"To determine if Ca 2+ was necessary for binding of CaM or CaM1,2,3,4 to TCF4B in vitro , we altered the Ca 2+ concentration in our protein lysates and performed additional co-immunoprecipitation experiments."
28289282
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"These results suggest that in vitro binding of TCF4B and CaM or CaM1,2,3,4 can occur independently of Ca 2+ binding, but that in vivo CaM-dependent prevention of pyramidal cell aggregation by TCF4B gain of function requires Ca 2+ , because the Ca 2+ insensitive CaM1,2,3,4 was ineffective at rescuing cellular aggregation."
28289282
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"At high cytosolic Ca 2+ levels in dark-adapted photoreceptors, xref we propose that a single Ca 2+ -saturated CaM binds to CNGB1 (highlighted red in xref ) in which the CaM N-lobe binds to the CaM1 site and the CaM C-lobe binds to CaM2 ( xref )."
36070238
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"The endothermic binding has much lower affinity with K d equal to 240 ± 30 μ M. The exothermic binding can be assigned as CaM1 binding to the CaM N-lobe because CaM1 binding to an isolated CaM N-lobe fragment (residues 1–80) is exothermic (Δ H = −2.5 ± 0.2 kcal/mol) with K d of 5.7 ± 1 μ M ( xref )."
36070238
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"The endothermic binding was verified as CaM1 binding to the CaM C-lobe because CaM1 binding to an isolated C-lobe fragment (residues 80–149) is endothermic (Δ H = +2.0 ± 0.3 kcal/mol) with K d of 29 ± 5 μ M ( xref )."
36070238
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"Thus, the ITC results indicate two separate CaM1 molecules bind to CaM to explain the 2:1 binding stoichiometry."
36070238
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"Efforts in our laboratories along this line have recently demonstrated that liver cell-CAM can bind calmodulin in a specific manner (Blikstad, unpublished observations) [27]."
2917610
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"Also, CaM1 binds to the CaM N-lobe with at least fivefold higher affinity compared to the CaM1 binding to the C-lobe."
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