IndraLab

Statements

CHUK phosphorylates NPM1. 8 / 8
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"Although there is currently no evidence to show that NPM-ALK induces the activation of the IKK-NF-kappaB pathway, the phosphorylation of NPM1 may be mediated by IKKalpha activated through the complex signaling pathway caused by NPM-ALK."
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sparser
"IKKα efficiently phosphorylated NPM Thr199A (Thr199 was replaced by alanine [ xref ]) ( xref )."
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"To determine whether IKKalpha phosphorylates NPM, we used different NPM deletion and mutation forms as kinase substrates and found that IKKalpha phosphorylated the amino acid (aa) 119-195 region, which contains five serine sites, but a kinase-dead IKKalpha mutant (IKKalpha-KA) did not phosphorylate NPM (XREF_FIG; XREF_SUPPLEMENTARY)."
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"Together, these results indicate that IKKα specifically phosphorylates S125-NPM."
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"IKKalpha interacted with and phosphorylated NPM, which further prevented centrosome amplification and genomic instability and inflammatory signaling activated IKKalpha to regulate centrosome duplication."
24290756
sparser
"To determine whether IKKα phosphorylates NPM, we used different NPM deletion and mutation forms as kinase substrates and found that IKKα phosphorylated the amino acid (aa) 119–195 region, which contains five serine sites, but a kinase-dead IKKα mutant (IKKα-KA) did not phosphorylate NPM ( xref ; xref )."
24290756
sparser
"Although CKII siRNA slightly reduced p-S125-NPM level in WT MEFs, the p-S125-NPM level inIkkα −/− cells was lower than that in WT cells lacking CKII ( xref ), suggesting that IKKα induced the phosphorylation of S125-NPM independently of CKII."
24290756
sparser
"S125A and a delta form lacking the aa 119–195 region completely abolished NPM phosphorylation by IKKα ( xref ), suggesting that IKKα phosphorylates S125 of NPM."
24290756