
IndraLab
Statements
Triton X-100 inhibits RELA. 1 / 1
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1
reach
"Cells were fixed with 4% paraformaldehyde for 30min, permeabilized in 0.2% Triton X-100 for 30min, washed with PBS, blocked with 1% BSA/0.05% Triton X-100 for 30min, and further incubated with rabbit polyclonal antibody against NF-kappaB p65 (1:100 dilution) in blocking buffer overnight."