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IndraLab

Statements

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phosphosite cbn pc11 biopax bel_lc signor biogrid tas lincs_drug hprd trrust | geneways tees isi trips rlimsp medscan sparser reach
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BRAF phosphorylates MAP2K1. 60 / 62
21 | 1 6 2 22 10
biopax:inoh
No evidence text available
biopax:inoh
No evidence text available
biopax:inoh
No evidence text available
reach
"We observed that 65% of anti-BRAF autoantibodies activate phosphorylation of MEK1 by BRAF in vitro."
reach
"BRAF directly phosphorylates the dual-specificity kinases MEK1 and MEK2, which in turn phosphorylate and activate the mitogen activated protein kinases, ERK1 and ERK2."
sparser
"We found that not BRAF WT but BRAF V600E dependent phosphorylation of MEK1 was increased in the presence of AA ( xref )."
reach
"BRAF proteins, Ras regulated kinase, phosphorylate MEK1 and MEK2, which in turn phosphorylate MAPK-ERK 1/2."
rlimsp
"Inhibitors may act by prevention of activation (PoA), which involves interfering with phosphorylation of nonactivated MEK1 by the upstream kinase, B-RAF."
biopax:panther
No evidence text available
sparser
"We envisioned two models for how MEK1 binding to KSR1 potentiates the ability of KSR1 to promote BRAF phosphorylation of MEK1."
biopax:inoh
No evidence text available
trips
"In response to epidermal growth factor, nerve growth factor, and platelet-derived growth factor, B-Raf and Raf-1 are activated, phosphorylate recombinant kinase-inactive MEK-1, and activate wild-type MEK-1."
rlimsp
"Phosphorylation of MEK1 by BRAF was detected by Western blotting and quantified."
biopax:panther
No evidence text available
reach
"Under normal circumstances, BRAF phosphorylates the MAPKs MEK1 and MEK2, which activate downstream signaling."
sparser
"We found that not BRAF WT but BRAF V600E-dependent phosphorylation of MEK1 was increased in the presence of AA ( Figure S5 D)."
reach
"MEK1 and 2 are phosphorylated and activated by B-RAF and a P124L mutation in MEK1 was identified through a MEK1 random mutagenesis screen that confers cellular resistance to the MEK inhibitor, AZD6244."
sparser
"MEK1 phosphorylation by BRAF was tested in the presence of purified anti-BRAF autoantibodies from RA patients or control antibody."
sparser
"Phosphorylation of MEK1 by BRAF was detected by Western blotting and quantified."
sparser
"Activated BRAF proteins phosphorylates and activates MEK1 and 2 (MAPKKs), which in turn phosphorylate and activate ERK1 and 2 (MAPKs)."
sparser
"We confirmed that B-Raf activated by cAMP phosphorylates the sites in MEK-1 (serines 218 and 222) that have previously been shown to be required for MEK activation by Raf-1 ( Alessi et al. 1994 )."
biopax:inoh
No evidence text available
sparser
"Immunoprecipitated BRAF(V600E) phosphorylated MEK1 protein in vitro ( xref , lane 5), however, treatment with 17-DMAG clearly inactivated the kinase activity of BRAF(V600E) ( xref , lane 6), even though equivalent levels of BRAF(V600E) protein were utilized from treated and non-treated cell lysates ( xref , lanes 3 and 4)."
reach
"Phosphorylation of MEK1 by BRAF was detected by Western blotting and quantified."
sparser
"BRAF V600E phosphorylates and activates the kinases MEK1 and MEK2, which in turn phosphorylate and activate the kinases ERK1 and ERK2, stimulating the MAPK pathway to promote cancer xref ."
sparser
"MEK1 and MEK2 are normally phosphorylated by BRAF and go on to activate downstream components of the mitogen-activated protein kinase pathway."
sparser
"Although both BRAF V600E or BRAF WT phosphorylates MBP but such phosphorylation was not affected in the presence of increasing concentrations of acetoacetate or 3-HB ( xref and xref ), suggesting that acetoacetate may specifically affect BRAF V600E dependent phosphorylation of MEK1."
biopax:inoh
No evidence text available
biopax:inoh
No evidence text available
biopax:inoh
No evidence text available
biopax:inoh
No evidence text available
sparser
"Fur- thermore, NGF treatment increases B-Raf autokinase activity but this is inhibited by cAME Importantly, in a reconstitution assay, B-Raf can phosphorylate and activate MEK-1."
sparser
"Under normal circumstances, BRAF phosphorylates the MAPKs MEK1 and MEK2, which activate downstream signaling."
reach
"Autoantibodies to BRAF activate the phosphorylation of MEK1 by BRAF."
biopax:inoh
No evidence text available
biopax:panther
No evidence text available
sparser
"Recent studies have suggested that KSR1 and 2 possess catalytic activity and that KSR2 participates in the assembly of a MEK1/KSR2/B-Raf ternary complex that is responsible for promoting rabbit MEK1 phosphorylation by mouse B-Raf ( xref , xref )."
biopax:inoh
No evidence text available
biopax:inoh
No evidence text available
reach
"In response to epidermal growth factor, nerve growth factor, and platelet derived growth factor, B-Raf and Raf-1 are activated, phosphorylate recombinant kinase-inactive MEK-1, and activate wild-type MEK-1."
rlimsp
"We observed that 65% of anti-BRAF autoantibodies activate phosphorylation of MEK1 by BRAF in vitro."
sparser
"HMGCL, however, selectively promotes BRAF V600E dependent phosphorylation and activation of MEK1 by controlling intracellular levels of its product AA, which specifically promotes BRAF V600E (but not BRAF WT) binding to MEK1 and subsequent MEK1 phosphorylation in cancer cells."
sparser
"Activated B-raf can then phosphorylate the dual-specificity kinases MEK1 and MEK2, which in turn activate ERK."
rlimsp
"B-Raf and Raf-1 both efficiently phosphorylate MAP kinase kinase (MEK-1)."
reach
"MEK1 phosphorylation by BRAF was tested in the presence of purified anti-BRAF autoantibodies from RA patients or control antibody."
biopax:panther
No evidence text available
rlimsp
"Autoantibodies to BRAF activate the phosphorylation of MEK1 by BRAF."
rlimsp
"MEK1 phosphorylation by BRAF was tested in the presence of purified anti-BRAF autoantibodies from RA patients or control antibody."
sparser
"We observed that 65% of anti-BRAF autoantibodies activate phosphorylation of MEK1 by BRAF in vitro ."
sparser
"MEK1 and MEK2 are normally phosphorylated by BRAF and go on to activate downstream components of the mitogen-activated protein kinase pathway."
sparser
"BRAF directly phosphorylates the dual-specificity kinases MEK1 and MEK2, which in turn phosphorylate and activate the mitogen-activated protein kinases, ERK1 and ERK2."
sparser
"Although both BRAF V600E or BRAF WT phosphorylates MBP, such phosphorylation was not affected in the presence of increasing concentrations of AA or 3-HB ( Figures 5 A, S5 E, and S5F), suggesting that[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
biopax:inoh
No evidence text available
biopax:panther
No evidence text available
biopax:inoh
No evidence text available
sparser
"BRAF(V600E) phosphorylates and activates the MEK1 and MEK2 kinases, which in turn phosphorylate and activate the ERK1 and ERK2 kinases, stimulating the mitogen-activated protein kinase (MAPK) pathway to promote cancer."
sparser
"Autoantibodies to BRAF activate the phosphorylation of MEK1 by BRAF."
biopax:inoh
No evidence text available
reach
"MEK1 and MEK2 are normally phosphorylated by BRAF and go on to activate downstream components of the mitogen activated protein kinase pathway."
biopax:inoh
No evidence text available