IndraLab

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BRAF phosphorylates MAP2K1. 56 / 58
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"We observed that 65% of anti-BRAF autoantibodies activate phosphorylation of MEK1 by BRAF in vitro."
20955560
reach
"BRAF proteins, Ras regulated kinase, phosphorylate MEK1 and MEK2, which in turn phosphorylate MAPK-ERK 1/2."
16404419
rlimsp
"Phosphorylation of MEK1 by BRAF was detected by Western blotting and quantified."
20955560
sparser
"We found that not BRAF WT but BRAF V600E-dependent phosphorylation of MEK1 was increased in the presence of AA ( Figure S5 D)."
26145173
reach
"BRAF, also known as serine/threonine protein kinase B-Raf, can phosphorylate MAP2K1 and thereby activates the MAP kinase signal transduction pathway in living cells."
33240890
sparser
"Activated BRAF proteins phosphorylates and activates MEK1 and 2 (MAPKKs), which in turn phosphorylate and activate ERK1 and 2 (MAPKs)."
19383316
sparser
"We confirmed that B-Raf activated by cAMP phosphorylates the sites in MEK-1 (serines 218 and 222) that have previously been shown to be required for MEK activation by Raf-1 ( Alessi et al. 1994 )."
9094716
reach
"When BRAF is inhibited, ERK can still be activated through CRAF, and BRAF binds to and phosphorylates MEK1 and MEK2 more efficiently than CRAF [XREF_BIBR], especially in his V600E/K mutated form."
33507915
sparser
"In the context of melanoma, oncogenic BRAF V600E constitutively phosphorylates and activates the MEK1 and MEK2 kinases, which in turn phosphorylate and activate the ERK1 and ERK2 kinases, hyperactivating the canonical Mitogen Activated Protein Kinase Pathway (MAPK) to promote uncontrolled melanocytes growth and caner initiation and progression xref ."
31317143
rlimsp
"Autoantibodies to BRAF activate the phosphorylation of MEK1 by BRAF."
20955560
rlimsp
"MEK1 phosphorylation by BRAF was tested in the presence of purified anti-BRAF autoantibodies from RA patients or control antibody."
20955560
sparser
"BRAF(V600E) phosphorylates and activates the MEK1 and MEK2 kinases, which in turn phosphorylate and activate the ERK1 and ERK2 kinases, stimulating the mitogen-activated protein kinase (MAPK) pathway to promote cancer."
24717435
reach
"MEK1 and MEK2 are normally phosphorylated by BRAF and go on to activate downstream components of the mitogen activated protein kinase pathway."
25037456
sparser
"After RAS engagement, RAF protein kinases RAF1, B-RAF, and A-RAF are activated and phosphorylate their dual-specificity protein kinase substrates MEK1 and MEK2 (also known as MAP2K1 and MAP2K2) [ xref ]."
30974877
sparser
"Subsequent to auto-phosphorylation, fully activated BRAF phosphorylates MEK1 to relay downstream signal."
29992710
sparser
"We found that not BRAF WT but BRAF V600E dependent phosphorylation of MEK1 was increased in the presence of AA ( xref )."
26145173
sparser
"We envisioned two models for how MEK1 binding to KSR1 potentiates the ability of KSR1 to promote BRAF phosphorylation of MEK1."
29433126
reach
"Under normal circumstances, BRAF phosphorylates the MAPKs MEK1 and MEK2, which activate downstream signaling."
27058947
sparser
"Phosphorylation of MEK1 by BRAF was detected by Western blotting and quantified."
20955560
reach
"Phosphorylation of MEK1 by BRAF was detected by Western blotting and quantified."
20955560
reach
"Using the same experimental approach, we leveraged the ~ 350-bp CRAF fragment library to identify the core binding site of MEK1, a downstream kinase that is phosphorylated by the RAF paralogs ARAF, BRAF, and CRAF."
33410398
sparser
"Both B-Raf-WT and B-Raf-ΔN phosphorylate MEK1 robustly, and for both, the deletion of the distal tail segment resulted in substantially lower levels of MEK1 phosphorylation, while the binding of ΔDTS mutants to 14-3-3 is unaffected ( xref , xref , xref and xref )."
31604311
sparser
"The data obtained by the biosensor experiments suggest that BRAF-phosphorylated MEK1 along with the MEK1 -S218D/S222D KinCon reporters occupy an active and opened kinase conformation, which is in accordance with the shown structural MD simulation data."
33808483
sparser
"HMGCL, however, selectively promotes BRAF V600E dependent phosphorylation and activation of MEK1 by controlling intracellular levels of its product AA, which specifically promotes BRAF V600E (but not BRAF WT) binding to MEK1 and subsequent MEK1 phosphorylation in cancer cells."
26145173
sparser
"MEK1 and MEK2 are normally phosphorylated by BRAF and go on to activate downstream components of the mitogen-activated protein kinase pathway."
25037456
sparser
"Although both BRAF V600E or BRAF WT phosphorylates MBP, such phosphorylation was not affected in the presence of increasing concentrations of AA or 3-HB ( Figures 5 A, S5 E, and S5F), suggesting that[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
26145173
reach
"MEK1 and MEK2 are normally phosphorylated by BRAF and go on to activate downstream components of the mitogen activated protein kinase pathway."
23994118
reach
"Fur- thermore, NGF treatment increases B-Raf autokinase activity but this is inhibited by cAME Importantly, in a reconstitution assay, B-Raf can phosphorylate and activate MEK-1."
7835430
reach
"BRAF directly phosphorylates the dual-specificity kinases MEK1 and MEK2, which in turn phosphorylate and activate the mitogen activated protein kinases, ERK1 and ERK2."
23477830
rlimsp
"Inhibitors may act by prevention of activation (PoA), which involves interfering with phosphorylation of nonactivated MEK1 by the upstream kinase, B-RAF."
18393446
reach
"MEK1 and 2 are phosphorylated and activated by B-RAF and a P124L mutation in MEK1 was identified through a MEK1 random mutagenesis screen that confers cellular resistance to the MEK inhibitor, AZD6244."
21593776
sparser
"Immunoprecipitated BRAF(V600E) phosphorylated MEK1 protein in vitro ( xref , lane 5), however, treatment with 17-DMAG clearly inactivated the kinase activity of BRAF(V600E) ( xref , lane 6), even though equivalent levels of BRAF(V600E) protein were utilized from treated and non-treated cell lysates ( xref , lanes 3 and 4)."
18676857
sparser
"However, in substrate phosphorylation assays, trametiglue not only inhibited MEK1 and MEK2, but also upstream kinases, including inhibition of both BRAF and CRAF phosphorylation of MEK1 as substrate ( xref , bottom)."
32927473
sparser
"MEK1 and MEK2 are normally phosphorylated by BRAF and go on to activate downstream components of the mitogen-activated protein kinase pathway."
23994118
sparser
"Although both BRAF V600E or BRAF WT phosphorylates MBP but such phosphorylation was not affected in the presence of increasing concentrations of acetoacetate or 3-HB ( xref and xref ), suggesting that acetoacetate may specifically affect BRAF V600E dependent phosphorylation of MEK1."
26145173
sparser
"Fur- thermore, NGF treatment increases B-Raf autokinase activity but this is inhibited by cAME Importantly, in a reconstitution assay, B-Raf can phosphorylate and activate MEK-1."
7835430
sparser
"Under normal circumstances, BRAF phosphorylates the MAPKs MEK1 and MEK2, which activate downstream signaling."
27058947
sparser
"Recent studies have suggested that KSR1 and 2 possess catalytic activity and that KSR2 participates in the assembly of a MEK1/KSR2/B-Raf ternary complex that is responsible for promoting rabbit MEK1 phosphorylation by mouse B-Raf ( xref , xref )."
28440493
reach
"In response to epidermal growth factor, nerve growth factor, and platelet derived growth factor, B-Raf and Raf-1 are activated, phosphorylate recombinant kinase-inactive MEK-1, and activate wild-type MEK-1."
7935374
rlimsp
"We observed that 65% of anti-BRAF autoantibodies activate phosphorylation of MEK1 by BRAF in vitro."
20955560
rlimsp
"B-Raf and Raf-1 both efficiently phosphorylate MAP kinase kinase (MEK-1)."
8090790
reach
"MEK1 phosphorylation by BRAF was tested in the presence of purified anti-BRAF autoantibodies from RA patients or control antibody."
20955560
sparser
"We observed that 65% of anti-BRAF autoantibodies activate phosphorylation of MEK1 by BRAF in vitro ."
20955560
biopax:panther
No evidence text available
sparser
"Autoantibodies to BRAF activate the phosphorylation of MEK1 by BRAF."
20955560
sparser
"BRAF, also known as serine/threonine-protein kinase B-Raf, can phosphorylate MAP2K1 and thereby activates the MAP kinase signal transduction pathway in living cells."
33240890
sparser
"When BRAF is inhibited, ERK can still be activated through CRAF, and BRAF binds to and phosphorylates MEK1 and MEK2 more efficiently than CRAF [ xref ], especially in his V600E/K mutated form."
33507915
trips
"In response to epidermal growth factor, nerve growth factor, and platelet-derived growth factor, B-Raf and Raf-1 are activated, phosphorylate recombinant kinase-inactive MEK-1, and activate wild-type MEK-1."
7935374
sparser
"MEK1 phosphorylation by BRAF was tested in the presence of purified anti-BRAF autoantibodies from RA patients or control antibody."
20955560
reach
"Activated BRAF proteins phosphorylates and activates MEK1 and 2 (MAPKKs), which in turn phosphorylate and activate ERK1 and 2 (MAPKs)."
19383316
sparser
"BRAF V600E phosphorylates and activates the kinases MEK1 and MEK2, which in turn phosphorylate and activate the kinases ERK1 and ERK2, stimulating the MAPK pathway to promote cancer xref ."
24717435
sparser
"BRAF phosphorylates MEK1 and/or MEK2, which in turn activates ERK1 and/or ERK2."
31723831
reach
"Autoantibodies to BRAF activate the phosphorylation of MEK1 by BRAF."
20955560
sparser
"Activated B-raf can then phosphorylate the dual-specificity kinases MEK1 and MEK2, which in turn activate ERK."
16996249
sparser
"BRAF directly phosphorylates the dual-specificity kinases MEK1 and MEK2, which in turn phosphorylate and activate the mitogen-activated protein kinases, ERK1 and ERK2."
23477830
sparser
"After interacting with GTP-bound RAS proteins, B-Raf phosphorylates the proteins MEK1 and MEK2, activating molecules that lead to cell proliferation."
35554338