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USP25 inhibits IFNB1. 19 / 20
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"(F) Effects of USP25 siRNA on SEV-induced activation of the IFN-β promoter."
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"Both USP21 and A20 inhibited SeV- and RIG-I-CARD-induced IFN-beta reporter activity in a dose dependent manner."
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"Interestingly, we found that overexpression of USP21 WT but not C221A mutant inhibited IFN-beta, NF-kappaB, and ISRE reporter activities in HEK293T cells in response to transfected poly (I : C), which was known to be mediated by MDA5 (unpublished data), suggesting that USP21 might also target MDA5."
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"Cellular ubiquitin specific proteases, USP21, USP3 and USP15, a subfamily of deubiqutinase, remove K63 linked polyubiquitin chains from RIG-I and block it to induce IFN-beta."
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"The results showed that USP25-WT remarkably inhibited SEV-induced activation of IFN-β (Figure 6A), IRF3 (Figure 6B), NF-κB (Figure 6C) and ISRE (Figure 6D) in a dose-dependent manner."
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"Reporter assays then indicated that knockdown of USP25 markedly potentiated SEV induced activation of the IFN-beta promoter (XREF_FIG)."
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"The results showed that USP25-WT remarkably inhibited SEV-induced activation of IFN-β ( Figure 6A ), IRF3 ( Figure 6B ), NF-κB ( Figure 6C ) and ISRE ( Figure 6D ) in a dose-dependent To further determine the levels at which USP25 negatively regulates type I IFN signaling, HEK-293T cells were transfected with DNA constructs encoding RIG-I, IPS-1, TRAF2, or TRAF6, together with IFN-β-Luc."
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"USP21 also inhibited RIG-I-CARD-induced IFN-beta reporter activity in WT, A20 -/-, ITCH -/-, and TAXBP1 -/- MEFs (unpublished data)."
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"In this screening, USP18 and USP21 significantly inhibited RIG-I-CARD-induced IFN-beta reporter activity, whereas other USPs had no effect or fewer effects (XREF_FIG)."
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"Fan et al., knowing that USP21 inhibits RIG-I-induced IFN-beta production, searched for its mechanism [XREF_BIBR]."
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"The results showed that USP25 significantly reduced SEV-induced IFN-β luciferase reporter activity (Figure 2A)."
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"Overexpression of RNF135 alone could not induce IFN-beta reporter activity, whereas once co-overexpressed with RIG-I, RNF135 robustly induced IFN-beta reporter activity, which was strongly inhibited by USP21 WT but not C222A mutant (XREF_FIG and not depicted)."
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"The results showed that USP25-WT remarkably inhibited SEV induced activation of IFN-beta (XREF_FIG), IRF3 (XREF_FIG), NF-kappaB (XREF_FIG) and ISRE (XREF_FIG) in a dose dependent manner."
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"Reporter assays then indicated that knockdown of USP25 markedly potentiated SEV-induced activation of the IFN-β promoter (Figure 2F)."
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"Furthermore, Knockdown of USP25 potentiated virus-induced induction of the IFN-β."
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"Empty vector (1 μg) or expression plasmid of USP25 (1 μg) were transfected into HEK-293T cells for 24 h and either untreated or treated with SEV for 16 h before real-time RT-PCR was performed.Figure 2: (A) USP25 inhibited SEV-induced activation of the IFN-β promoter in a dose-dependent manner."
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"Overexpression of USP25 resulted in reduced IFN-beta promoter activity and IFN-beta expression in SeV infected or poly (I : C)-treated cells, whereas gene silencing of USP25 enhanced IFN-beta promoter activity [XREF_BIBR]."
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"Furthermore, human USP21 inhibited SeV induced IFN-beta reporter activities in MEFs whereas mouse USP21 inhibited SeV induced IFN-beta reporter activities in HEK293T cells (XREF_FIG and not depicted), suggesting that USP21 function in antiviral signaling is not limited by cell type and is conserved between human and mouse."
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"We further found that overexpression of USP25 efficiently reduced SEV induced IFN-beta induction."
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