IndraLab

Statements

ATF3 activates USP7. 3 / 4
| 3
reach
"Whereas USP7 indeed increased the Tip60 expression level and concurrently decreased the amount of ubiquitinated Tip60, ATF3 expression promoted the effects of USP7 on Tip60 expression (XREF_FIG, lanes 3 vs. lane 2, and lane 5 vs. lane 4) and ubiquitination (XREF_SUPPLEMENTARY, lane 3 vs. lane 2), suggesting that ATF3 might promote USP7 mediated deubiqutination of Tip60."
25865756
reach
"However, it was unlikely that ATF3 directly regulates the USP7 deubiquitinase activity as pre-incubation of ATF3 with the ubiquitinated Tip60 protein, but not with USP7, appeared to be required for the ATF3 deubiquitination promoting effect as revealed by the in vitro ubiuiqitination assays (XREF_SUPPLEMENTARY)."
25865756
reach
"As expected, recombinant USP7 catalyzed removal of ubiquitin moieties from Tip60 (XREF_FIG, lane 2 vs. lane 1), and this effect was largely enhanced by ATF3 (XREF_FIG, lane 3)."
25865756