IndraLab

Statements

AKT phosphorylates NOS3 on S1177. 188 / 191
1 4 | 107 73 3
sparser
"Akt directly phosphorylates eNOS at Ser 1177 resulting in increased eNOS activity and subsequent NO production."
18775359
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"This effect was apparently due to decreased eNOS interaction with its positive protein regulator HSP90 (but not increased interaction with caveolin-1), which decreases AKT activation and AKT mediated eNOS phosphorylation on Ser 1177, and reduces constitutive NOS activity."
21143412
reach
"Posttranslational regulation of eNOS involves multiple mechanisms, including the phosphorylation of eNOS by Akt at Ser1177."
18599867
sparser
"The serine/threonine kinase Akt (protein kinase B) directly phosphorylates eNOS at Ser1177 (human eNOS) and Ser1179 (bovine eNOS), which increases eNOS activity several-fold [12,13,31] ."
12559774
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"Recent studies suggest that Akt is activated by phosphorylation via activated PI3K and phosphorylates eNOS on serine 1177, thereby activating this enzyme."
25391146
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"In contrast, other groups found no change of eNOS expression in aging, but observed decreased Akt dependent phosphorylation of eNOS at Ser1177 with increasing age as a potential explanation for an impaired endothelial dysfunction in the elderly [XREF_BIBR]."
26184181
sparser
"Compared with 18 matched control, ET in 17 stable CAD patients significantly improved endothelial function, which may be closely related to shear-stress induced phosphorylation of eNOS at Ser 1177 residue by AKT [ xref ]."
| PMC
sparser
"And Dimmeler et al. demonstrated that Akt phosphorylated the Serine 1177 site of eNOS protein and that enhanced eNOS activity; they inhibited the PI3K/Akt pathway and that led to prevention of eNOS activation [ xref ], and Fulton et al. had similar findings [ xref ]."
24949428
reach
"In addition, inhibition of AKT activation by LY294002 (10 muM) inhibited eNOS Ser1177 phosphorylation by TRX (XREF_FIG)."
28179506
sparser
"Akt directly phosphorylates eNOS at Ser 1177 resulting in increased eNOS activity and subsequent NO production."
23306778
sparser
"Regarding the regulation of eNOS by PI3K/Akt signaling, it has previously been shown that activated Akt phosphorylates eNOS on Ser1177, enhancing both basal and stimulated eNOS enzyme activity, and thereby NO release [ xref , xref ]."
24708830
sparser
"Akt directly phosphorylates eNOS of the Ser1177 residue, resulting in increased eNOS activity and subsequent NO production [ xref , xref ]."
32187237
reach
"Phosphorylation of eNOS Ser1177 by PI3K and Akt cascade is a typical signal transduction pathway by which eNOS activity is enhanced."
25908301
reach
"This activation of endothelial Akt has been shown to stimulate phosphorylation of eNOS at serine residue 1177, known to be an important regulatory mechanism leading to eNOS activation."
22431312
sparser
"AKT phosphorylates eNOS at Ser1177, resulting in increased nitric oxide production and vasodilation."
26297582
reach
"PI3K signaling mediates Akt and PKB phosphorylates eNOS at serine 1177 residue XREF_BIBR - XREF_BIBR."
21203503
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"ENOS phosphorylation at serine 1177 is mediated by Akt XREF_BIBR, which has been extensively shown to be modulated by oxidative stress."
20479943
reach
"Insulin stimulates calmodulin and HSP90 binding to eNOS which facilitates insulin stimulated phosphorylation and activation of eNOS at Ser 1177 by Akt."
22684034
sparser
"Moreover, bradykinin has been associated with liberation of eNOS from inhibitory binding domains at the sarcolemma [17] and linked to eNOS serine 1177 phosphorylation by Akt [9] , leading to an inc[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12606259
sparser
"In isolated aorta, vasodilation involves NO release (Sahin et al., xref ) through a mechanism linked to the activation of endothelial NO synthase (eNOS) via Akt-dependent phosphorylation of eNOS at Ser 1177 (Lembo et al., xref ; Vecchione et al., xref )."
22679436
sparser
"The protein kinase Akt phosphorylates eNOS at serine 1177 in vivo , whereas other kinases such as members of the family AGC (protein kinase A, protein kinase G, protein kinase C) as well as AMP-activated kinase (AMPK) can phosphorylate eNOS at the same site in vitro and perhaps in vivo xref ."
33042519
reach
"In addition, B1R is capable of activating the PI3 and Akt pathway, which phosphorylates eNOS in serine 1177 [XREF_BIBR - XREF_BIBR]."
32306971
reach
"It has been reported that eNOS phosphorylation by PI3K and Akt pathway is required for efficient NO production [XREF_BIBR, XREF_BIBR], after phosphorylated at Ser 473 by PI3K, Akt in turn phosphorylates eNOS at Ser 1177 and thereby increases eNOS activity, and finally enhances NO production."
25874925
sparser
"This requires HSP90 binding to eNOS which facilitates insulin-stimulated activation of eNOS mediated by phosphorylation of eNOS at Ser 1177 by Akt."
18775359
reach
"Agonists of histamine H1 receptors lead to eNOS activation through the increase in intracellular calcium, while estrogen receptors signaling involves protein kinase B (Akt)-mediated Ser 1177 phosphorylation of eNOS [43]."
| PMC
reach
"Akt directly phosphorylates eNOS at Ser 1177 resulting in increased eNOS activity and subsequent NO production."
18775359
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"Akt directly phosphorylates eNOS at Ser 1177, resulting in increased eNOS activity and NO production [XREF_BIBR]."
23573411
reach
"Erectile function of aged rats can lastingly be enhanced by long-term inhibition of phosphodiesterase (PDE) 5 with sildenafil by rescuing Akt dependent phosphorylation of eNOS (Ser 1177) in the penis."
19342700
sparser
"After the growth factors bind to their receptors, the phosphatidylinositol-3-OH-kinase (PtdIns (3) K) activates Akt, and Akt phosphorylates eNOS at serine 1177 [ xref ]."
26075871
reach
"Akt phosphorylates eNOS at the activating site Ser 1177."
22266027
reach
"It was shown that the production of NO increased in vascular endothelial cells as a consequence of insulin stimulated phosphorylation of eNOS at Ser1177 by Akt [XREF_BIBR]."
26132171
reach
"It has been shown that HDL induced eNOS activation is mediated by protein kinase Akt and PKB or MAPK, which phosphorylates eNOS at Ser 1177 [30,31]."
15979078
sparser
"For example, eNOS phosphorylation at Ser1177 by phosphatidylinositol 3-kinase (PI3-K)-dependent Akt plays a critical role in eNOS activation ( xref )."
28413728
reach
"In isolated aorta, vasodilation involves NO release through a mechanism linked to the activation of endothelial NO synthase (eNOS) via Akt dependent phosphorylation of eNOS at Ser 1177."
22679436
reach
"Upon stimulation, Akt and protein kinase B (PKB) phosphorylates eNOS at Ser 1177 (1179) and possibly Ser 615 (617), and consequently activates eNOS [XREF_BIBR]."
25460366
sparser
"In previous studies it has been shown that eNOS activation in response to HDL is mediated via Akt-dependent eNOS phosphorylation at Ser1177 [ xref ]."
23781332
sparser
"Akt could phosphorylate eNOS at Ser1177 and increase NOS activation, thereby leading to cardioprotection against ischemia/reperfusion ( Bellis et al., 2009 ; Changjun Yang and Murugesan Velayutham, 20[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
29705606
reach
"It attenuated phosphorylation of IRS-1 at S307 and effectively ameliorated the tyrosine phosphorylation of IRS-1, which activated PI3K, subsequently activated PI3K phosphorylates and activated downstream target Akt which directly phosphorylated eNOS at Ser1177, leading to increased production of NO(Hwang et al., 2011)."
35058775
reach
"The protein kinase Akt directly phosphorylated eNOS at Ser1177."
29632805
reach
"Previous studies have proposed that VEGF stimulated eNOS phosphorylation at Ser1177 is mediated by PKB XREF_BIBR, yet complete inhibition of PKB with the PI3K inhibitor, wortmannin only partially inhibited VEGF stimulated NO production XREF_BIBR."
17276402
reach
"It was also noted that the ERalpha, associated with the membrane at the caveolae level, activates the pathway PI3K and AKT, which, in turn, leads to phosphorylation on serine 1177 (S1177) of eNOS [XREF_BIBR, XREF_BIBR]."
29805728
sparser
"Akt phosphorylates eNOS at the activating site Ser 1177 ( xref )."
22266027
reach
"The activation of eNOS by statins, ROCK inhibitors, and Akt is most likely mediated through phosphorylation of eNOS at serine 1177 (serine 1179 in bovine eNOS), the major phosphorylation site by Akt."
19673606
reach
"The resulting increased Akt activation leads to enhanced eNOS phosphorylation at Ser 1177 and/or Ser 1179, thereby increasing the NO synthesizing activity."
30050658
sparser
"Aortic eNOS and iNOS protein expressions, Akt activity, and eNOS phosphorylation by Akt at serine 1177 were up-regulated in cirrhotic rats."
16012955
reach
"The activation of PI3K / Akt phosphorylates eNOS at serine 1177, leading to the elevation of NO production in endothelial cells [XREF_BIBR]."
28542422
sparser
"Akt phosphorylates eNOS on Ser1177 or Thr308, directly increasing the enzyme activity and also enhancing the response to other activators of eNOS [133,134] ."
24794552
reach
"A simple model has been proposed that activated Akt could phosphorylate eNOS at Ser 1177 to stimulate NO production."
30135105
reach
"Akt directly phosphorylates eNOS at Ser 1177 resulting in increased eNOS activity and subsequent NO production."
23306778
reach
"Although it is recognized that eNOS phosphorylation at Ser 1177 by the PI3K-Akt pathway plays a central role in the response to shear stress [XREF_BIBR, XREF_BIBR, XREF_BIBR], the contribution of eNOS translocation and the requirement of Ca 2+ in this physiological regulation are controversial."
24217770
reach
"With increasing disease severity, more malondialdehyde is bound to HDL particles and subsequent activation of protein kinase-betaII (PKC-betaII) results in pronounced phosphorylation of eNOS at its inhibitory site Thr 495 whereas the eNOS activating phosphorylation at Ser 1177, mediated by PI3K and Akt, is reduced."
27563480
reach
"ENOS is phosphorylated at serine 1177 (S1177) by Akt kinase and other kinases [XREF_BIBR - XREF_BIBR], discussed in detail below."
19647446
reach
"To confirm that H 2 S induced NO production was dependent on eNOS phosphorylation, pharmacological inhibition of Akt was used to prevent phosphorylation of eNOS at Ser 1177."
22194727
sparser
"This regulation may occur via phosphorylation of eNOS at the serine 1177 residue by AKT (Dimmeler et al., xref ; Fulton et al., xref )."
22144946
reach
"LSS stimulates Akt phosphorylation, which in turn phosphorylates eNOS at Ser 1177, leading to eNOS activation and NO production 54."
24876354
sparser
"We next investigated if SC79 could induce Akt-dependent phosphorylation of eNOS at the activating site S1177 in A549s."
| PMC
sparser
"Dimmeler et al. demonstrated that Akt phosphorylated the Serine 1177 site of eNOS protein and enhanced eNOS activity."
26539501
sparser
"In the aged rat penis Akt-dependent phosphorylation of Ser-1177 on eNOS is decreased, whereas phosphorylation of Thr-495 on eNOS is increased, in spite of increased eNOS protein expression ( xref )."
19342700
sparser
"Agonists of histamine H1 receptors lead to eNOS activation through the increase in intracellular calcium, while estrogen receptors signaling involves protein kinase B (Akt)-mediated Ser 1177 phosphorylation of eNOS [ xref ]."
| PMC
reach
"It has been shown that HDL induced eNOS activation is mediated by protein kinase Akt and PKB or MAPK, which phosphorylates eNOS at Ser 1177 [30,31]."
15979078
sparser
"Akt kinase phosphorylates eNOS at S1177 and activates it, as discussed below."
19647446
reach
"Compared with 18 matched control, ET in 17 stable CAD patients significantly improved endothelial function, which may be closely related to shear-stress induced phosphorylation of eNOS at Ser 1177 residue by AKT [85]."
| PMC
sparser
"Akt directly phosphorylates eNOS at Ser 1177 , resulting in increased eNOS activation and NO production."
22709426
reach
"Phosphorylation of eNOS on serine 1177 residue (S1177) by activated Akt is included as shown in Figure XREF_FIG."
29899706
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"Akt phosphorylation of ser1177 on eNOS is a major regulatory mechanism, enhancing the production of NO at any concentration of Ca2+."
23720270
sparser
"Akt induced eNOS phosphorylation at serine 1177 is an important mechanism to protect heart against IR injury ( xref ; xref )."
27445803
reach
"H 2 S enhances Akt activity, possibly through PTEN inhibition (Greiner 2014), triggering eNOS phosphorylation on Ser1177 and increased NO production."
26318600
reach
"AKT leads to phosphorylation of eNOS on serine 1177."
24400038
reach
"First, the PI3K pathway members, including its downstream molecule, Akt, are essential regulators; activated Akt directly phosphorylates ser1177 on eNOS, enhancing the binding activity of [Ca 2+]/calmodulin [XREF_BIBR]."
33562632
sparser
"Further, we showed that preserved vascular redox state during aging decreases age-related hypertension by the following mechanisms: (i) maintenance of NO-mediated relaxing responses, (ii) preservation of functional NO release, (iii) activated AKT-dependent eNOS Ser1177 phosphorylation, (iv) decreased eNOS-dependent generation of O 2 •− in arteries of Trx-Tg mice because of preservation of eNOS function during aging, (v) abrogation of eNOS glutathionylation in the TAS of Trx-Tg mice, and (vi) decreased arterial stiffness with improved vascular flow."
28179506
sparser
"AKT can directly phosphorylated eNOS at Ser1177 and activate the enzyme [ xref , xref , xref ]."
19320971
reach
"On the other hand, phosphorylation of eNOS by Akt at Ser1177 increases the NO production."
21705677
signor
"Recently many investigators have shown that protein phosphorylation of enos by several serine/threonine kinases is a critical control step for no production by endothelial cells. Phosphorylation by amp kinase, akt (or protein kinase b), or protein kinase a on serine 1179 (bovine) or serine 1177 (human) of enos leads to enhanced activity of the enzyme and, thus, augmented production of no."
11729179
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"XREF_BIBR, XREF_BIBR Interestingly, whereas we now show that in response to CRP, Src activation leads to eNOS antagonism, the stimulation of eNOS by numerous agonists including HDL, estrogen and shear stress also entails Src activation, which preceeds PI3 kinase and Akt activation that promotes eNOS Ser1177 phosphorylation."
21940940
reach
"We found that purified Akt phosphorylated cardiac eNOS at Ser1177, resulting in activation of eNOS."
10469573
sparser
"Akt phosphorylates of NOS3 at the serine 1177 (S1177) which reduces dissociation of calmodulin at low Ca i levels, such that normal basal Ca i is sufficient to increase NO production."
25314136
sparser
"This is thought to occur via phosphorylation of eNOS at the serine 1177 residue by Akt[ xref , xref ], required for the VEGF induced endothelial cell migration[ xref ]."
28163831
reach
"The VEGFR2 has been shown to be activated by shear stress independently of the presence of its ligand VEGF and leads to activation of phosphoinositide 3-kinase (PI 3 K), the serine specific protein kinase Akt, which in turn causes phosphorylation of human eNOS at serine 1177."
27927026
sparser
"Activated Akt phosphorylates human eNOS at serine 1177 and subsequently increases NOS activity."
15082754
sparser
"Previous evidence indicated a crucial for endothelial nitric oxide synthase (eNOS) in diabetes ( xref ), whereas recent reports suggested that Akt signaling stimulates NO production via the Akt-dependent phosphorylation of eNOS at Ser1177 ( xref , xref , xref )."
26447102
sparser
"On the other hand, phosphorylation of eNOS by Akt at Ser1177 increases the NO production ( xref )."
21705677
sparser
"Previous research showed that protein kinase B (AKT) and AMP‐activated protein kinase (AMPK) could phosphorylate eNOS at the Ser1177 site in response to various stimuli. xref , xref In our study, p‐eNOS (Ser1177) expression dramatically increased with zingerone treatment after AB surgery in vivo and after PE challenge in vitro, thus leading to increased production of NO."
31293067
rlimsp
"AKT can directly phosphorylated eNOS at Ser1177 and activate the enzyme [1,28,29]."
19320971
reach
"Additionally, an abundance of evidence has shown that Akt can activate eNOS by directly phosphorylating eNOS at serine 1177 XREF_BIBR."
29104511
sparser
"The phosphorylation of Ser 1177 of eNOS by Akt is dependent on statin-mediated recruitment of Akt to the eNOS complex by hsp90 chaperone protein [ xref – xref ]."
16375664
reach
"Dimmeler et al. demonstrated that Akt phosphorylated the Serine 1177 site of eNOS protein and enhanced eNOS activity."
26539501
phosphoelm
No evidence text available
16043647
reach
"Shear stress, VEGF, insulin, estrogens, statins and leptin stimulate eNOS S1177 phosphorylation by Akt kinase activation [XREF_BIBR, XREF_BIBR, XREF_BIBR, XREF_BIBR]."
19647446
sparser
"There are also groups who report unchanged eNOS expression in aged vessels, but instead report decreases in Akt-dependent phosphorylation of eNOS Ser1177 ."
33670865
reach
"Protein kinase B (Akt) is then activated to phosphorylate eNOS at Ser1177, leading to NO production."
33758788
reach
"Activated Akt phosphorylates human eNOS at serine 1177 and subsequently increases NOS activity."
15082754
reach
"Activated p38 MAPK induced Akt-dependent phosphorylation of eNOS at Ser1177."
32046324
sparser
"Reduced eNOS mRNA and protein expression, decreased phosphorylation of eNOS at Ser-1177 by serine-threonine protein kinase Akt, increased dissociation of functional eNOS dimers into nonfunctional monomers, and reduced NOS activity have been noticed in CC of rat or mouse models of T2DM, all of which would lead to decreased NO production. xref xref xref xref xref xref xref Consistent with previous reports, we also found an impaired Akt-eNOS pathway in CC of T2DM rats, including reduced eNOS expression at both transcription and protein level, decreased phosphorylation of Akt at Thr308 and phosphorylation of eNOS at Ser-1177, as well as subsequent reduced total NOS activity and NO production."
30226217
reach
"Akt directly phosphorylates eNOS at Ser 1177, resulting in increased eNOS activation and NO production."
22709426
rlimsp
"We demonstrated that high-fat diet intake led to a noteworthy decrease in eNOS phosphorylation at Ser1177 residue through the Akt pathway, which seems to be compensated by upregulation of phosphorylation at Ser615, but without an increment in nitric oxide production."
24105250
sparser
"The phosphorylation of eNOS at Ser1177 by Akt is dependent on statin-mediated recruitment of Akt to eNOS complex by heat shock protein 90 (hsp90) chaperone protein."
16410222
sparser
"Besler et al. showed that HDL isolated from patients with CAD (CAD-HDL) fails to stimulate Akt-dependent phosphorylation of eNOS ser1177 but instead enhances inhibitory phosphorylation of eNOS Thr495 [94] ."
33924941
sparser
"The most widely studied phosphorylation pathway involves the serine/threonine Akt (also known as protein kinase B), which can phosphorylate eNOS on Ser 1177 , resulting in activation of the enzyme and increased production of NO [ xref , xref ]."
21671887
reach
"We tested the hypothesis that impaired nitric oxide (NO)-mediated, endothelium dependent dilation in aged soleus muscle feed arteries (SFA) is due to an age related decline in the potential for PI3-kinase (PI3K)/protein kinase B (Akt)-dependent phosphorylation of endothelial NO synthase (eNOS) on serine residue 1177 (p-eNOS (ser1177))."
23563601
sparser
"As a hallmark of eNOS activation, phosphorylation of eNOS at Ser1177 induced by activated protein kinase B (PKB/Akt) is pivotal for NO production."
33993653
reach
"The vascular endothelial growth factor (VEGF) contributes to activation of eNOS by Ca2 +/calmodulin and also stimulates the protein kinase Akt, which directly phosphorylates eNOS on Ser1177 and increases enzyme activity."
15375304
reach
"Akt can directly phosphorylate eNOS on S1177 and promote its function."
34319011
reach
"PI3K signaling mediates Akt and PKB phosphorylates eNOS at serine 1177 residue XREF_BIBR - XREF_BIBR."
21203503
reach
"The serine/threonine kinase Akt (protein kinase B) directly phosphorylates eNOS at Ser1177 (human eNOS) and Ser1179 (bovine eNOS), which increases eNOS activity several-fold [12,13,31]."
12559774
reach
"Furthermore, over-expression of dominant negative Akt [Akt (DN)] significantly reduced LA induced eNOS phosphorylation (XREF_FIG), whereas an AMPK inhibitor, dorsomorphin, reduced LA induced phosphorylation of AMPKalpha but not eNOS (XREF_FIG), strongly supporting that LA induced eNOS phosphorylation at Ser1177 is mediated by Akt."
25460366
reach
"Akt directly phosphorylates eNOS at Ser 1177 resulting in increased eNOS activity and subsequent NO production."
22684034
reach
"The endothelial dependent vasodilator effect of insulin involves Akt mediated phosphorylation of eNOS on residue Ser 1177, resulting in enhanced NO production."
18835939
reach
"Because NO bioavailability promotes EPC functions and maturation, previous studies by many others showed that the phosphorylation of eNOS Ser 1177 by PI3K-Akt is a key molecular event leading to eNOS derived NO."
18599796
sparser
"PLC-γ associated intracellular Ca 2+ flux is associated with calmodulin-mediated activation of eNOS ( xref ), but eNOS activation may also be directly mediated by AKT phosphorylation of eNOS on S 1177 in Ca 2+ -independent manner ( xref )."
20084057
reach
"The most widely studied phosphorylation pathway involves the serine/threonine Akt (also known as protein kinase B), which can phosphorylate eNOS on Ser 1177, resulting in activation of the enzyme and increased production of NO [XREF_BIBR, XREF_BIBR]."
21671887
reach
"There are also groups who report unchanged eNOS expression in aged vessels, but instead report decreases in Akt dependent phosphorylation of eNOS Ser1177."
33670865
reach
"Upon stimulation, Akt and protein kinase B (PKB) phosphorylates eNOS at Ser 1177 (1179) and possibly Ser 615 (617), and consequently activates eNOS [XREF_BIBR]."
25460366
reach
"Aortic eNOS and iNOS protein expressions, Akt activity, and eNOS phosphorylation by Akt at serine 1177 were up-regulated in cirrhotic rats."
16012955
reach
"Phosphorylation of eNOS serine residue 1177 (S1177, equivalent to murine S1176 and bovine S1179) by Akt strongly boosts its activity and NO production."
32903816
reach
"In our palmitate induced model of oxidative stress, we demonstrated diminished NO production via reduced Akt dependent phosphorylation of eNOS at Ser 1177, which supports the findings of previous reports; however, the damaging effect of palmitate was attenuated when HUVECs were coincubated with EMPs."
28687612
reach
"Akt phosphorylates eNOS at Ser 1177 in the C-terminal reductase domain, which increases electron flow and augments calcium-CaM sensitivity of the enzyme."
26286023
sparser
"AKT kinase phosphorylates eNOS at Ser1177 and activates it, leading to the production of nitric oxide (NO)."
26297582
sparser
"Akt directly phosphorylates eNOS at Ser 1177 resulting in increased eNOS activity and subsequent NO production ( xref )."
22684034
reach
"Akt could phosphorylate eNOS at Ser1177 and increase NOS activation, thereby leading to cardioprotection against ischemia and reperfusion (Bellis et al., 2009; Changjun Yang and Murugesan Velayutham, [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
29705606
reach
"In summary, the present series of experiments demonstrated that dietary salt intake directly promoted a complex endothelial cell signaling cascade that induced Akt mediated phosphorylation of NOS3 at S1176 and augmented NO production."
18981321
rlimsp
"Long-term high-fat consumption leads to downregulation of Akt phosphorylation of eNOS at Ser1177 and upregulation of Sirtuin-1 expression in rat cavernous tissue."
24105250
sparser
"A simple model has been proposed that activated Akt could phosphorylate eNOS at Ser 1177 to stimulate NO production ( xref )."
30135105
reach
"Our observations may account for the formerly reported phenomenon that active Akt rescued phosphorylation of eNOS at Ser1177 in the presence of constitutively active RhoA without restoring eNOS activi[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17651694
reach
"Protein kinases including PI3K, Akt, AMPK, CaMKII, PKA and cyclic GMP dependent protein kinase are reported to phosphorylate eNOS Ser1177 in response to different stimuli [XREF_BIBR]."
25908301
reach
"In the AKT/eNOS axis, AKT mediates eNOS phosphorylation at Ser1177, leading to an elevation in eNOS activity."
34253242
sparser
"Phosphorylation of eNOS on serine 1177 residue (S1177) by activated Akt is included as shown inFigure xref ."
29899706
sparser
"First, the PI3K pathway members, including its downstream molecule, Akt, are essential regulators; activated Akt directly phosphorylates ser1177 on eNOS, enhancing the binding activity of [Ca 2+ ]/calmodulin [ xref ]."
33562632
sparser
"Activated AMPK and Akt phosphorylate eNOS-Ser1177 activation site, which in turn stimulates NO production."
32879422
sparser
"Phosphorylation of eNOS at serine-1177 by Akt (protein kinase B) activates the enzyme, which itself is active when phosphorylated."
31581464
sparser
"Insulin, leptin, vascular endothelial growth factor (VEGF), estrogens, statins, and shear stress increase eNOS S1176 phosphorylation by Akt kinase [ xref – xref ]."
23291238
phosphoelm
No evidence text available
12791703
sparser
"It is known that eNOS is activated by the Akt dependent phosphorylation of eNOS-ser 1177 whereas PKCδ has been shown to be responsible for phosphorylation of the eNOS-thr 495 inhibitory site xref , xref ."
23166614
reach
"Phosphorylation of eNOS by Akt and protein kinase B at serine 1177 increases eNOS activity, while Hsp90 promotes increased eNOS activity via direct interaction with the enzyme."
21388435
reach
"Activation of VEGFR2 in endothelial cells (by VEGF binding) results in the Akt dependent phosphorylation of eNOS at Ser 1177 XREF_BIBR, XREF_BIBR, while VEGF treatment of BAECs resulted in the phosphorylation of eNOS at Ser 617 and Ser 635 (equivalent to human Ser 615 and Ser 633) XREF_BIBR."
28433660
sparser
"Phosphorylation of eNOS at Ser 1177 by AKT leads to increased NO production. xref , xref Thus the concentration-dependent decrease in eNOS phosphorylation seen in our study would be expected to lead to reduced NO production."
21139021
reach
"Also, we did not confirm mediatory role of activated AKT or ERK1/2 on estradiol induced eNOS phosphorylation on Ser 1177."
20233295
reach
"Agonist induced phosphorylation of eNOS on serine 1177 was attenuated by Akt inhibition, whereas association of eNOS with actin was not."
21741389
reach
"Regarding the regulation of eNOS by PI3K and Akt signaling, it has previously been shown that activated Akt phosphorylates eNOS on Ser1177, enhancing both basal and stimulated eNOS enzyme activity, and thereby NO release [XREF_BIBR, XREF_BIBR]."
24708830
sparser
"Phosphorylation of eNOS at its Ser 1177 activation site by the serine/threonine-kinase Akt and consequently increased NO release have been identified as the main signaling pathway."
27463705
sparser
"In conclusion, our data demonstrate that, following application of eNOS agonists, eNOS binds to the globular form of β-actin – an event which is dependent on the integrity of the Ser1177 residue – and[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
21741389
sparser
"Akt directly phosphorylates eNOS at Ser 1177 , resulting in increased eNOS activity and NO production [ xref ]."
23573411
sparser
"Our data support previous reports and also specifically implicate mitochondrial superoxide in Akt-dependent eNOS phosphorylation at serine 1177."
20479943
sparser
"The phosphorylation of eNOS at Ser1177 by AKT increases the production of NO ( xref ), which results in vasodilation."
33995040
reach
"Akt activation downstream of PI3K causes eNOS phosphorylation at S1177 and upregulates NO production in A549, primary nasal epithelial cells, primary human type II AECs, H441 distal lung epithelial cells, and primary human macrophages."
| PMC
reach
"This effect is mediated primarily by Akt dependent phosphorylation of eNOS at serine 1177 XREF_BIBR, XREF_BIBR."
20479943
reach
"AKT kinase phosphorylates eNOS at Ser1177 and activates it, leading to the production of nitric oxide (NO)."
26297582
reach
"Akt phosphorylates eNOS at serine 1177, which directly increases activity and also enhances the response to other activators [XREF_BIBR - XREF_BIBR]."
20186491
sparser
"The data in the present study suggest a novel mechanism of endogenous H 2 S signaling: up-regulation of NO production via Akt-dependent phosphorylation of eNOS at Ser1177, although the mechanism by which H 2 S activates Akt is unknown."
22194727
sparser
"The PI3K/Akt/eNOS pathway plays an important role in the regulation of NO production and it has been shown that activated Akt phosphorylates eNOS at Ser1177, stimulates eNOS activity and induces NO release. xref , xref Furthermore, the activity of eNOS is influenced not only by the phosphorylation of eNOS‐Ser1177 but also by the phosphorylation of eNOS‐Thr495 and thus we also assessed the phosphorylation eNOS‐Thr495."
30770623
reach
"Independent from its antioxidant activity, HO-1 interacted with endothelial nitric oxide synthase (eNOS), promoting the interaction of eNOS and Akt, thus enhancing eNOS phosphorylation at Ser1177 by Akt, subsequently increasing nitric oxide production [9]."
34312365
reach
"Akt, a serine/threonine kinase, also appears to have a central role in Ang 1-7 signaling, and phosphorylation of eNOS at Ser1177 by Akt results in a twofold increase in eNOS catalytic activity [XREF_BIBR]."
25874965
sparser
"Phosphorylation of eNOS is a key regulator for its activity and regulates NO production in endothelial cells and vascular tone.( xref )Akt can phosphorylate eNOS-Ser1177 which leads to increase eNOS activity."
22798709
sparser
"AKT phosphorylates eNOS at Ser1177, resulting in increased nitric oxide production and vasodilation."
28427390
reach
"Protein kinase B (Akt) or AMP-activated protein kinase (AMPK) have been previously identified as the kinases responsible for the classical HDL-induced eNOS phosphorylation and activation at Ser1177 [15]."
31817387
reach
"Statins induce Akt mediated phosphorylation of eNOS at Ser1177, which results in nitric oxide production, but with no accompanying change in the total level of eNOS protein."
22117815
reach
"The PI3K and AKT signaling pathway is known to regulate eNOS activity, and activation of AKT has been shown to stimulate phosphorylation of eNOS at Ser1177 [XREF_BIBR]."
24804268
reach
"Akt phosphorylates eNOS at Ser 1177 and induces NO synthesis XREF_BIBR, XREF_BIBR."
19374334
reach
"After the growth factors bind to their receptors, the phosphatidylinositol-3-OH-kinase (PtdIns (3) K) activates Akt, and Akt phosphorylates eNOS at serine 1177 [XREF_BIBR]."
26075871
reach
"GPCR is also coupled to downstream kinases like PI3K and Akt, which induces eNOS phosphorylation at Ser 1177 and eNOS activation [XREF_BIBR]."
24180383
reach
"Previous evidence indicated a crucial for endothelial nitric oxide synthase (eNOS) in diabetes, whereas recent reports suggested that Akt signaling stimulates NO production via the Akt dependent phosphorylation of eNOS at Ser1177."
26447102
reach
"Akt kinase phosphorylates eNOS at S1177 and activates it, as discussed below."
19647446
sparser
"It was shown that the production of NO increased in vascular endothelial cells as a consequence of insulin-stimulated phosphorylation of eNOS at Ser1177 by Akt [ xref ]."
26132171
reach
"The results of immunoblotting and immunolabeling indicated that rhVEGF-C activates eNOS via the PI3K-Akt pathway, which directly phosphorylates eNOS to form p-eNOS (Ser1177)."
29632805
sparser
"Activated Akt phosphorylates eNOS at Ser 1177 , a major activation site ( xref ; xref ; xref ), which is enhanced by its interaction with heat shock protein 90 ( xref ; xref ; xref )."
20045558
sparser
"It has been reported that eNOS phosphorylation by PI3K/Akt pathway is required for efficient NO production [ xref , xref ], after phosphorylated at Ser-473 by PI3K, Akt in turn phosphorylates eNOS at Ser-1177 and thereby increases eNOS activity, and finally enhances NO production."
25874925
reach
"PECAM-1 has no mechanosensing properties, but plays an important role in the delayed Ca 2+ -independent eNOS activation in response to shear, which seems to involve Akt dependent phosphorylation of eNOS by serine 1177, as demonstrated in PECAM-1-deficient mice."
27927026
reach
"Long-term high-fat consumption leads to downregulation of Akt phosphorylation of eNOS at Ser1177 and upregulation of Sirtuin-1 expression in rat cavernous tissue."
24105250
reach
"Akt phosphorylates eNOS on Ser1177 or Thr308, directly increasing the enzyme activity and also enhancing the response to other activators of eNOS [133,134]."
24794552
sparser
"Importantly, activated AMPK and Akt phosphorylate the eNOS-Ser1177 activation site, which in turn stimulates NO production."
32887916
reach
"Phosphorylation of eNOS at Ser 1177 by AKT leads to increased NO production."
21139021
sparser
"The protein kinase Akt directly phosphorylated eNOS at Ser1177."
29632805
sparser
"On estrogen stimulation, PI3K rapidly phosphorylates the downstream serine/threonine kinase Akt, activated Akt in turn phosphorylates serine 1177 of NOS-3 in endothelial cells xref , xref ."
23209733
reach
"Given the effects of wortmannin and Ad.alpha1DN, we propose that VEGF stimulates both PKB and AMPK mediated phosphorylation of eNOS at Ser1177 under normoxic conditions."
17276402
reach
"We next investigated if SC79 could induce Akt-dependent phosphorylation of eNOS at the activating site S1177 in A549s."
| PMC
reach
"ROCK negatively regulates the function of endothelial nitric oxide synthase eNOS (NOS3) by inhibiting AKT phosphorylation of eNOS on Ser 1177 leading to decreased NO production."
32285223
reach
"AKT phosphorylates eNOS at Ser1177, resulting in increased nitric oxide production and vasodilation."
28427390
sparser
"VEGF is one of the most potent pro-angiogenic factors which mediates the phosphorylation of eNOS at the serine 1177 residue by AKT and a consequent increase in NO production [ xref , xref , xref ]."
33321789
reach
"Injection of EGCG plus eriodictyol significantly increased Akt activity (XREF_FIG), enhanced phosphorylation of eNOS at Ser1177 (XREF_FIG), phosphorylation of PKCdelta at Ser662 (corresponding to human PKCdelta Ser664) (XREF_FIG) and ASM activity (XREF_FIG)."
25824377
reach
"The data in the present study suggest a novel mechanism of endogenous H 2 S signaling : up-regulation of NO production via Akt dependent phosphorylation of eNOS at Ser1177, although the mechanism by which H 2 S activates Akt is unknown."
22194727
sparser
"Akt, which is activated by phosphorylation via activated PI3K, phosphorylates eNOS on serine 1177 (p-eNOS), thereby activating this enzyme [ xref , xref ]."
32192513
reach
"This uncoupling effect is mediated primarily by Akt dependent phosphorylation of eNOS at serine 1177 [XREF_BIBR, XREF_BIBR], which alters the kinetics of electron transfer within the enzyme, leading to increased production of superoxide, particularly at low levels of calcium [XREF_BIBR]."
22654724
phosphoelm
No evidence text available
12446767
reach
"AKT phosphorylates eNOS at Ser1177, resulting in increased nitric oxide production and vasodilation."
26297582
sparser
"The phosphorylation of eNOS at Ser1177 (p-eNOS S1177 ) by AKT activates this enzyme."
33741019
phosphoelm
No evidence text available
10376603
sparser
"Besler et al. showed that HDL isolated from patients with CAD (CAD-HDL) fails to stimulate Akt-dependent phosphorylation of eNOS ser1177 but instead enhances inhibitory phosphorylation of eNOS Thr495 [ xref ]."
33924941
sparser
"VEGF has been reported to stimulate endothelial cell migration by activating Akt which in turn phosphorylates Ser1177 residue of eNOS [ xref , xref ]."
20569463
reach
"IRS1 activates the PI3K and Akt, which, in turn, phosphorylates eNOS at Ser1177, catalyzing the conversion of L-arginine to L-citrulline and NO [XREF_BIBR]."
28750629